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1301 - 1350 of 2352 citations since 2019

• A modular platform for on-demand vaccine self-assembly enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens

  Kevin B. Weyant, et al., bioRxiv - Synthetic Biology 2021

  Quote: ... and 1-oleoyl-2-[12-biotinyl(aminododecanoyl)]-sn-glycero-3-phosphocholine (18:1-12:0 biotin PC) powder was purchased from Avanti Polar Lipids. The biotin-GD2 ...
• Label-free tracking and mass measurement of single proteins on lipid bilayers

  Eric D. B. Foley, et al., bioRxiv - Biophysics 2021

  Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC; 850375P) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) (DOPS; 840035P) were purchased from Avanti Polar Lipids. GTP (NU-1012 ...
• Towards the NMR solution Structure and the Dynamics of the C-terminal Region of APOL1 and its G1, G2 Variants with a Membrane Mimetic

  Sethu M. Madhavan, et al., bioRxiv - Biophysics 2021

  Quote: ... Initial detergent screening for NMR was done using dodecylphosphocholine (DPC, Avanti Polar Lipids, Alabaster, AL), sodium dodecyl sulfate (SDS ...
• Molecular Determinants of Complexin Clamping in Reconstituted Single-Vesicle Fusion

  Manindra Bera, et al., bioRxiv - Biophysics 2021

  Quote: ... All the lipids used in this study were purchased from Avanti Polar Lipids (Alabaster, AL). ATTO647N-DOPE was purchased from ATTO-TEC ...
• Investigating the conformational dynamics of SARS-CoV-2 NSP6 protein with emphasis on non-transmembrane 91-112 & 231-290 regions

  Amit Kumar, et al., bioRxiv - Biophysics 2021

  Quote: ... following which the lipids were extruded 25 times through the mini extruder (Avanti Polar Lipids, Inc. USA) through cut off filter of 100nm polycarbonate membrane to prepare uniform Large unilamellar vesicles (LUV).
• Strategies for the site-specific decoration of DNA origami nanostructures with functionally intact proteins

  Joschka Hellmeier, et al., bioRxiv - Biophysics 2021

  Quote: ... and 2% 1,2-dioleoyl-sn-glycero-3-[N(5-amino-1-carboxypentyl) iminodiacetic acid]succinyl[nickel salt] (Ni-DOGS NTA) (Avanti Polar Lipids) were used and SLBs were formed as described above ...
• Strategies for the site-specific decoration of DNA origami nanostructures with functionally intact proteins

  Joschka Hellmeier, et al., bioRxiv - Biophysics 2021

  Quote: ... vesicles containing 100% 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) (Avanti Polar Lipids) were prepared at a total lipid concentration of 0.5mg ml-1 as described26 in 10x Dulbecco’s phosphate-buffered saline (PBS ...
• Towards the NMR solution Structure and the Dynamics of the C-terminal Region of APOL1 and its G1, G2 Variants with a Membrane Mimetic

  Sethu M. Madhavan, et al., bioRxiv - Biophysics 2021

  Quote: ... micelles and also using 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC)/1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bicelles (Avanti Polar Lipids, Alabaster, AL). Proteins bound to the Ni2+ column were washed with 40 column volumes (CV ...
• Hydration layer of only few molecules controls lipid mobility in biomimetic membranes

  Madhurima Chattopadhyay, et al., bioRxiv - Biophysics 2021

  Quote: ... 23-(dipyrrome-theneboron difluoride)-24-norcholesterol (TopFluor cholesterol) were purchased from Avanti Polar Lipids, Alabaster AL. ...
• Preparation of Asymmetric Vesicles with Trapped CsCl Avoids Osmotic Imbalance, Non-Physiological External Solutions, and Minimizes Leakage

  Ming-Hao Li, Daniel P. Raleigh, Erwin London, bioRxiv - Biophysics 2021

  Quote: ... and 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) were purchased from Avanti Polar Lipids (Alabaster, AL). Lipids were dissolved in chloroform and stored at −20°C ...
• Membrane surfaces regulate assembly of a ribonucleoprotein condensate

  Wilton T. Snead, et al., bioRxiv - Biophysics 2021

  Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (DOPE cap-biotin) were purchased from Avanti Polar Lipids.
• Single-virus content mixing assay reveals cholesterol-enhanced influenza membrane fusion efficiency

  Katherine N. Liu, Steven G. Boxer, bioRxiv - Biophysics 2021

  Quote: ... and cholesterol (CH) were purchased from Avanti Polar Lipids (Alabaster, AL). Texas Red-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE) ...
• Super-resolution imaging of highly curved membrane structures in giant vesicles encapsulating molecular condensates

  Ziliang Zhao, et al., bioRxiv - Biophysics 2021

  Quote: ... 2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) were purchased from Avanti Polar Lipids and cholesterol from Sigma-Aldrich ...
• 3D single molecule localization microscopy reveals the topography of the immunological synapse at isotropic precision below 15 nm

  Lukas Velas, et al., bioRxiv - Biophysics 2021

  Quote: ... and 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-arboxypentyl)iminodiacetic acid)succinyl] (nickel salt) (Ni-NTA-DGS) were from Avanti Polar Lipids, Inc ...
• Dual effects of presynaptic membrane mimetics on α-synuclein amyloid aggregation

  Yuxi Lin, et al., bioRxiv - Biophysics 2021

  Quote: ... and 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) were obtained from Avanti Polar Lipids Inc ...
• Single-Molecule Manipulation of Macromolecules on Membranes Using High-Resolution Optical Tweezers

  Yukun Wang, Huaizhou Jin, Yongli Zhang, bioRxiv - Biophysics 2021

  Quote: ... All lipids were purchased from Avanti Polar Lipids, Inc ...
• Structural anatomy of C1 domain interactions with DAG and other agonists

  Sachin S. Katti, et al., bioRxiv - Biophysics 2021

  Quote: Chloroform solutions of long-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and short-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) (Avanti Polar Lipids), or their deuterated versions d54-DMPC (Avanti Polar Lipids ...
• Structural anatomy of C1 domain interactions with DAG and other agonists

  Sachin S. Katti, et al., bioRxiv - Biophysics 2021

  Quote: ... The DAG chemical shifts were obtained using the 13C-1H HSQC spectrum of the 0.4 mM [13C,C1-C3]-racemic DAG (custom synthesized by Avanti Polar Lipids) in 10 mM d38-dodecylphosphocholine (DPC ...
• Structural anatomy of C1 domain interactions with DAG and other agonists

  Sachin S. Katti, et al., bioRxiv - Biophysics 2021

  Quote: ... and 14-doxyl PC were obtained from Avanti Polar Lipids. The lipid concentrations of final bicelle preparations were measured using the phosphate determination assay41.
• Structural anatomy of C1 domain interactions with DAG and other agonists

  Sachin S. Katti, et al., bioRxiv - Biophysics 2021

  Quote: ... or their deuterated versions d54-DMPC (Avanti Polar Lipids) and d40-DHPC (Cambridge Isotope Laboratories) ...
• Time evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA

  A. Gallud, et al., bioRxiv - Biophysics 2021

  Quote: ... and 1,2-distearoyl-sn- glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] (DSPE-PEG2000- biotin) lipids were from Avanti Polar Lipids, 1,2-dimyristoyl-sn-glycero-3- phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DMPE-PEG2000 ...
• Colicin E1 opens its hinge to plug TolC

  S. Jimmy Budiardjo, et al., bioRxiv - Biophysics 2021

  Quote: POPC (Avanti Polar Lipids) in chloroform was dried under a stream of nitrogen and freeze dried to remove residual chloroform ...
• Ion-dependent structure, dynamics, and allosteric coupling in a non-selective cation channel

  Adam Lewis, et al., bioRxiv - Biophysics 2021

  Quote: ... d54-DMPC (Avanti Polar Lipids) was used for methyl labeled samples ...
• Hypoxia-directed tumor targeting of CRISPR/Cas9 and HSV-TK suicide gene therapy using lipid nanoparticles

  Alicia Davis, Kevin V. Morris, Galina Shevchenko, bioRxiv - Cancer Biology 2021

  Quote: ... and 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (PEG-DMG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). 2,2-dilinoleyl-4-(2-dimethylaminoethyl)-[1,3]-dioxolane (DLin-KC2-DMA ...
• mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cells

  Shanna Dewaele, et al., bioRxiv - Cancer Biology 2021

  Quote: ... All other lipids were purchased from Avanti Polar Lipids, Inc ...
• Transport among protocells via tunneling nanotubes

  Ingrid Jin Schanke, et al., bioRxiv - Biophysics 2021

  Quote: ... coli polar extract (Avanti Polar Lipids, USA) (50:50 wt %) ...
• Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble using a Polarized Vortex Point Spread Function

  Tianben Ding, Matthew D. Lew, bioRxiv - Biophysics 2021

  Quote: ... A 25-passage extrusion (Avanti Polar Lipids, 610000) was performed on a hot plate (95°C ...
• Single-molecule Dynamic In-Solution Inhibition Assay: A Method for Full Kinetic Profiling of Drug Candidate Binding to GPCRs in Native Membranes

  Tim Kaminski, Vladimir P. Zhdanov, Fredrik Höök, bioRxiv - Biophysics 2021

  Quote: ... The supernatant was then supplemented with protease inhibitor and extruded through a 100-nm pore filter using an Avanti Mini Extruder (Avanti Polar Lipids), seven times ...
• Allosteric modulation of the adenosine A_2A receptor by cholesterol

  Shuya Kate Huang, et al., bioRxiv - Biophysics 2021

  Quote: ... The concentrations of 3β-F-cholesterol and F7-cholesterol (Avanti Polar Lipids) were estimated via integration of 19F NMR resonances of the cholesterol analog in relation to a reference compound (fluoroacetate in the case of 3β-F-cholesterol and trifluoroacetate in the case of F7-cholesterol) ...
• Correlation of mitochondrial TOM core complex stop-and-go and open-closed channel dynamics

  Shuo Wang, et al., bioRxiv - Biophysics 2021

  Quote: ... and covered with a lipid/oil solution containing 9.5 mg/ml 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC, Avanti Polar Lipids, USA) and 1:1 (v:v ...
• Transport among protocells via tunneling nanotubes

  Ingrid Jin Schanke, et al., bioRxiv - Biophysics 2021

  Quote: ... 1 wt % of lipid conjugated fluorophore 16:0 Rhod Liss PE (Avanti Polar Lipids, USA) was added into the lipid mixture ...
• Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family

  Karthik Ramanadane, et al., bioRxiv - Biophysics 2021

  Quote: ... POPG (Avanti Polar Lipids) at a weight ratio of 3:1 ...
• Lipid Tails Modulate Antimicrobial Peptide Membrane Incorporation and Activity

  Lawrence R. Walker, Michael T. Marty, bioRxiv - Biophysics 2021

  Quote: ... The sample was extruded (Avanti Polar Lipids, Alabaster, AL) using a 100 nm polycarbonate Track-Etched filter (GE Healthcare) ...
• Lipid Tails Modulate Antimicrobial Peptide Membrane Incorporation and Activity

  Lawrence R. Walker, Michael T. Marty, bioRxiv - Biophysics 2021

  Quote: ... and 1-stearoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (SOPG) lipids were purchased from Avanti Polar Lipids (Alabaster, AL). Indolicidin ...
• An Immunogold Single Extracellular Vesicular RNA and Protein (^AuSERP) Biochip to Predict Responses to Immunotherapy in Non-Small Cell Lung Cancer Patients

  Luong T. H. Nguyen, et al., bioRxiv - Immunology 2021

  Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, Avanti Polar Lipids), and Bis(1,2-distearoyl-sn-glycero-3-phosphoethanolamine)-N-[(polyethylene glycol)-2000] (Bis-DSPE PEG2000 ...
• An Immunogold Single Extracellular Vesicular RNA and Protein (^AuSERP) Biochip to Predict Responses to Immunotherapy in Non-Small Cell Lung Cancer Patients

  Luong T. H. Nguyen, et al., bioRxiv - Immunology 2021

  Quote: ... and Bis(1,2-distearoyl-sn-glycero-3-phosphoethanolamine)-N-[(polyethylene glycol)-2000] (Bis-DSPE PEG2000, Avanti Polar Lipids) (50:30:18:2 mole ratio in 200 proof ethanol) ...
• An Immunogold Single Extracellular Vesicular RNA and Protein (^AuSERP) Biochip to Predict Responses to Immunotherapy in Non-Small Cell Lung Cancer Patients

  Luong T. H. Nguyen, et al., bioRxiv - Immunology 2021

  Quote: ... an aqueous solution of the PD-L1 ssDNA oligo was mixed with a lipid formulation of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE, Avanti Polar Lipids), linoleic acid (LA ...
• An Immunogold Single Extracellular Vesicular RNA and Protein (^AuSERP) Biochip to Predict Responses to Immunotherapy in Non-Small Cell Lung Cancer Patients

  Luong T. H. Nguyen, et al., bioRxiv - Immunology 2021

  Quote: ... which was made by replacing Bis-DSPE PEG2000 with DSPE-PEG(2000) Biotin (Avanti Polar Lipids) in the lipid formulation of CLN-MB fabrication ...
• An Immunogold Single Extracellular Vesicular RNA and Protein (^AuSERP) Biochip to Predict Responses to Immunotherapy in Non-Small Cell Lung Cancer Patients

  Luong T. H. Nguyen, et al., bioRxiv - Immunology 2021

  Quote: ... an aqueous solution of MBs in PBS was vigorously mixed with a lipid formulation of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP, Avanti Polar Lipids, AL, USA), Cholesterol (Sigma-Aldrich) ...
• An Immunogold Single Extracellular Vesicular RNA and Protein (^AuSERP) Biochip to Predict Responses to Immunotherapy in Non-Small Cell Lung Cancer Patients

  Luong T. H. Nguyen, et al., bioRxiv - Immunology 2021

  Quote: ... 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG 2000, Avanti Polar Lipids) (50:48:2 mole ratio in 200 proof ethanol) ...
• Small proline-rich proteins (SPRRs) are epidermally produced antimicrobial proteins that defend the cutaneous barrier by direct bacterial membrane disruption

  Chenlu Zhang, et al., bioRxiv - Immunology 2021

  Quote: ... Lipids were then thawed and passed through a 100 nm pore membrane using a mini-extruder kit (Avanti Polar Lipids #610000) and purified on a PD-10 column to remove excess dye ...
• Selective Display of a Chemoattractant Agonist on Cancer Cells Activates the Formyl Peptide Receptor 1 on Immune Cells

  Eden L. Sikorski, et al., bioRxiv - Immunology 2021

  Quote: ... The resulting large multilamellar vesicles were freeze-thawed for seven cycles and subsequently extruded through a polycarbonate membrane with 100 nm pores using a Mini-Extruder (Avanti Polar Lipids) to produce large unilamellar vesicles (LUVs) ...
• A Plasmodium falciparum lysophospholipase regulates fatty acid acquisition for membrane biogenesis to enable schizogonic asexual division

  Pradeep Kumar Sheokand, et al., bioRxiv - Microbiology 2021

  Quote: ... Then each lipid content was normalized according to the parasite cell number and a C21:0 internal standard (Avanti Polar lipids). All analyses were performed in triplicate or more (n=3) ...
• IgG surface mobility promotes antibody dependent cellular phagocytosis by Syk and Arp2/3 mediated reorganization of Fcγ receptors in macrophages

  Seongwan Jo, et al., bioRxiv - Immunology 2021

  Quote: ... Small unilamellar vesicles (SUVs) were prepared by mixing DSPE-PEG(2000)-Biotin (880129 Avanti Polar Lipids) and DPPC (850355 ...
• The co-stimulatory activity of Tim-3 requires Akt and MAPK signaling and immune synapse recruitment

  Shunsuke Kataoka, et al., bioRxiv - Immunology 2021

  Quote: ... and 0.2% biotin-CAP-PE (Avanti Polar Lipids) were deposited on glass coverslips cleaned with piranha solution (50:50 mixture of 30% H2O2 and 96% H2SO4) ...
• Maternal diet-induced obesity during pregnancy alters lipid supply to fetuses and changes the cardiac tissue lipidome in a sex-dependent manner

  Lucas C. Pantaleão, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Lipid standards were purchased from Avanti Polar lipids (Alabaster ...
• Probing neuropeptide volume transmission in vivo by a novel all-optical approach

  Hejian Xiong, et al., bioRxiv - Neuroscience 2021

  Quote: ... Afterwards the suspension was extruded through 200 and 100 nm polycarbonate membranes (Whatman, USA) for 21 passages each using a Mini Extruder (Avanti Polar Lipids, USA). Free calcein or SST was removed by size-exclusion chromatography with Sephacryl® S-1000 column (Cat ...
• Probing neuropeptide volume transmission in vivo by a novel all-optical approach

  Hejian Xiong, et al., bioRxiv - Neuroscience 2021

  Quote: 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC, 63-89-8, >99%) and cholesterol (ovine wool, 57-88-5, >98%) were purchased from Avanti Polar Lipids, Inc ...
• A novel mutation (E83Q) unlocks the pathogenicity of human alpha-synuclein fibrils and recapitulates its pathological diversity

  Senthil T. Kumar, et al., bioRxiv - Neuroscience 2021

  Quote: ... was purchased from Avanti Polar Lipids, Inc ...
• SARS-CoV-2 Fusion Peptide has a Greater Membrane Perturbating Effect than SARS-CoV with Highly Specific Dependence on Ca²⁺

  Alex L. Lai, Jack H. Freed, bioRxiv - Molecular Biology 2021

  Quote: ... 10PC and 14PC and the head group spin label dipalmitoylphospatidyl-tempo-choline (DPPTC) were purchased from Avanti Polar Lipids (Alabaster, AL) or synthesized in our laboratory according to previous protocols ...