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Citations for Avanti Polar Lipids :
51 - 100 of 562 citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... This solution is then passed through a 100nm Nucleopore Track-Etched Membranes in an extruder (Avanti Polar Lipids), and sonicated.
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bioRxiv - Biophysics 2022Quote: ... the mixture was extruded 15 times through a 100 nm membrane using the Mini Extruder (Avanti Polar Lipids). This yielded a liposome stock solution of 100 nm liposomes with 4.0 mM lipid in 10 mM Tris pH 7 ...
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bioRxiv - Pathology 2021Quote: ... The liposomal suspension was homogenized to 100 nm unilamellar vesicles with an extruder and appropriate membranes (Avanti Polar Lipids), yielding a clear suspension.
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bioRxiv - Cell Biology 2021Quote: ... The lipid mixture was extruded through polycarbonate membranes of 100 nm size cut-off (Avanti Polar Lipids, AL, USA), a minimum of 21 times to form liposomes ...
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bioRxiv - Biophysics 2023Quote: ... the lipid vesicles were extruded through a 100 nm pore-sized polycarbonate membrane filter (Avanti Polar Lipids, Alabaster, AL). Flow channels on the surface of a biotin-PEG coated microchannel were injected with 1 mg/mL concentrated lipid solution and incubated for 45 minutes to deposit a bilayer (Fig ...
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bioRxiv - Biophysics 2024Quote: ... and then extruded 17 times through a polycarbonate membrane with 100 nm pores using a miniextruder (Avanti Polar Lipids) at the same temperature ...
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bioRxiv - Microbiology 2022Quote: ... 10 mM Hepes pH 7.5 and extrusion through 400 nm pore-size membranes using a mini-extruder (Avanti Polar Lipids). The lipid concentration of liposome preparations was estimated by comparing the level of rhodamine fluorescence in the liposome sample relative to samples of rehydrated lipids of known concentration ...
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bioRxiv - Biophysics 2023Quote: ... The suspension was extruded through polycarbonate membranes with a 50 nm pore diameter using Avanti Mini Extruder (Avanti Polar Lipids) to obtain unilamellar liposomes ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The formed SUVs were then extruded first through a 0.4 μm, then a 0.1 μm membrane (Whatman, Nucleopore, UK) using the Mini Extruder from Avanti Polar Lipids Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... The final proteoliposomes solution was then extruded by passing 11 times through a 400-nm membrane filter (Avanti Polar Lipids). These final proteoliposomes were kept at 4°C and used immediately ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 pmol cardiolipin (14:1/14:1/14:1/15:1, Avanti Polar Lipids), and 50 pmol monolysocardiolipin (16:0/16:0/16:0 ...
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bioRxiv - Cell Biology 2020Quote: ... The PC-PIP2 suspension was then extruded through polycarbonate membranes with a pore size of 100 nm according to manufacturer’s instructions (mini-extruder, Avanti Polar Lipids) and resulting PC-PIP2 large unilamellar vesicles LUVs (40 mg/mL ...
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bioRxiv - Biophysics 2022Quote: ... The lipid mixture was then extruded through a 100-nm polycarbonate membrane 21 times using a mini-extruder (Avanti Polar Lipids). Lipid vesicles were used immediately following extrusion.
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bioRxiv - Cell Biology 2021Quote: ... The lipid suspension was extruded through polycarbonate membranes (0.1 μm) using the Avanti Mini-Extruder (Avanti Polar Lipids, Alabaster, AL, USA).
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bioRxiv - Cell Biology 2021Quote: ... Scraped lysates were precleared by centrifugation at 2000 g for 10 min at 4°C and the microsomal membranes were solubilized by incubation in the presence of 30 mM 1,2-diheptanoyl-sn-glycero-phosphocholine (DHPC, Avanti Polar Lipids) for 5 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The large unilamellar phospholipid vesicles of ~100nm diameter were prepared by extruding rehydrated phospholipid suspensions through two stacked 0.1 μm polycarbonate membranes (Avanti Polar Lipids).
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bioRxiv - Biophysics 2019Quote: ... The liposome solution was sonicated in a bath sonicator for 30 s and extruded through a 0.1 µm membrane (Whatman) using a mini-extruder (Avanti Polar lipids). Extra-vesicular ANTS were removed with a 10 ml desalting column (PD-10 ...
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bioRxiv - Immunology 2020Quote: ... large unilamellar vesicles were prepared by extrusion (> 11 passes) through a 0.1 µm membrane at room temperature using a mini-extruder (Avanti Polar Lipids). Liposomes were used immediately after preparation.
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bioRxiv - Biochemistry 2022Quote: ... The rehydrated lipid solutions were extruded 21 times through a 100 nm diameter pore size polycarbonate membrane (Avanti Polar Lipids Inc.). The extruded solutions were stored at 4°C and consumed within a week after preparation.
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bioRxiv - Cell Biology 2023Quote: ... Liposomes were passed through polycarbonate membrane with a pore size of 100 nm using the MiniExtruder (Avanti Polar Lipids, AL, USA) at 65°C ...
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bioRxiv - Biophysics 2023Quote: ... The solutions were then extruded at least 21 times through a 200 nm cut off polycarbonate membrane filter (Avanti Polar Lipids). Vesicles were then filtered using PD-10 desalting columns (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... The resulting vesicles were extruded ten times through a 100 nm polycarbonate membrane using a commercial mini-extruder (Avanti Polar Lipids).
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bioRxiv - Biophysics 2024Quote: ... The resulting multilamellar vesicle solutions were extruded through a 100 nm diameter polycarbonate membrane in a Mini extruder (Avanti Polar Lipids). The LUVs were prepared in 50 µM NaCl solution ...
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bioRxiv - Biochemistry 2023Quote: ... and 22:0 (1:1:1) (Avanti Polar Lipids, Inc) was dissolved in ethanol/dodecane (98:2 ...
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bioRxiv - Biochemistry 2023Quote: ... and 16:0 (1:1:1) (Avanti Polar Lipids, Inc) was dissolved in ethanol ...
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bioRxiv - Cancer Biology 2020Quote: ... coli total lipid extract for the reconstitution of proteins was from Avanti Polar Lipids, Inc ...
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bioRxiv - Biochemistry 2022Quote: ... 25 pmol CL 57:4 (14:1/14:1/14:1/15:1, Avanti Polar Lipids) and 25 pmol MLCL (16:0/16:0/16:0 ...
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bioRxiv - Immunology 2021Quote: ... The resulting large multilamellar vesicles were freeze-thawed for seven cycles and subsequently extruded through a polycarbonate membrane with 100 nm pores using a Mini-Extruder (Avanti Polar Lipids) to produce large unilamellar vesicles (LUVs) ...
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bioRxiv - Cell Biology 2022Quote: ... aLDs were extruded 11 times through polycarbonate filters (Nuclepore Track-Etch Membrane, Whatman) with a pore diameter of 100 nm using a mini-extruder (Avanti Polar Lipids). The size was verified by dynamic light scattering measurements on a DynaPro (Protein Solutions) ...
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bioRxiv - Immunology 2021Quote: ... The lipid film was hydrolyzed with PBS buffer and sized to 1,000 nm in a mini-extruder equipped with a 1,000 nm pore polycarbonate membrane (Avanti Polar Lipids, Alabama, AL). The diameter of liposomes was checked by dynamic light scattering (DLS) ...
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bioRxiv - Microbiology 2019Quote: ... The sample was then extruded 11 times (65 °C) through polycarbonate membrane (pore size of 200 nm) with a syringe extrusion device (Avanti Polar Lipids) after which the sample was quickly cooled down and stored in a refrigerator ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting LUV solution was passed more than 20 times through a polycarbonate filter membrane with a pore size of 100 nm using the extruder from Avanti Polar Lipids, Inc ...
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bioRxiv - Biophysics 2019Quote: ... Nuclepore track-etch membrane (50 nm) from Whatman and filter support disks 10 mm from Whatman were purchased from Avanti Polar Lipids Inc (Alabaster ...
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bioRxiv - Biophysics 2021Quote: ... lipids were formed by fusion of small uni-lamellar vesicles (SUV) that were prepared by lipid extrusion using a membrane with 100 nm pore size (cat. no. 610000, Avanti Polar Lipids). SLBs were formed by addition of 10 μl of SUV mix (at 4 mM lipid concentration ...
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bioRxiv - Biophysics 2020Quote: ... Uni-lamellar vesicles were formed via ten cycles of freeze-thawing followed by extrusion ten times) through a polycarbonate membrane with pores sizes of 100 nm using a mini-extruder (Avanti Polar Lipids) kept 50 °C on a hotplate ...
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bioRxiv - Biochemistry 2022Quote: ... Vesicles were extruded through two nucleopore polycarbonate membranes with a pore size of 200 nm using a mini-extruder (Avanti Polar Lipids), yielding large unilamellar vesicles (LUVs).
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bioRxiv - Biophysics 2022Quote: ... the liposome solution was passed 11 times through a polycarbonate membrane with a 400 nm pore size in a mini extruder (Avanti Polar Lipids). Total lipid concentration was diluted with buffer to 0.16 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Unilamellar liposomes were produced by seven freeze-thaw cycles (30 sec in liquid nitrogen followed by 5 min in a 37°C water bath) and extrusion (at least 21 times) through a polycarbonate filter with 100 nm pore size (polycarbonate membranes from Avanti Polar Lipids). The liposomes were then stored on ice.
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bioRxiv - Bioengineering 2022Quote: ... smegmatis and then co-extruded nine times through a 100 nm pore size polycarbonate porous membrane using a mini extruder (Avanti Polar Lipids). Excess OMVs and soluble compounds were removed from the supernatant after centrifugation at 15000 g for 10 minutes at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Small unilamellar vesicles at 1mg/mL were formed by 31 extrusion cycles using polycarbonate membranes of 50nm pore size (Avanti Polar Lipids). This solution was used directly to form the supported lipid bilayer.
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bioRxiv - Biochemistry 2019Quote: ... The resulting large multilamellar vesicles containing RJbinder or RJcontrol were freeze-thawed for seven cycles and subsequently extruded through a polycarbonate membrane with 100 nm pores using a Mini-Extruder (Avanti Polar Lipids) to produce large unilamellar vesicles.
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bioRxiv - Cell Biology 2020Quote: ... Liposomes were then extruded 21 times through a polycarbonate membrane with a 50◻nm pore size (Mini-Extruder, Avanti Polar Lipids). Extruded liposomes were centrifuged at 18,000 g for 5 min to remove insoluble material and stored at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The large unilamellar liposome vesicles were made by extruding the suspension through a 400nm polycarbonate membrane filter using a mini extruder (Avanti Polar Lipids). To reconstitute proteoliposomes ...
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bioRxiv - Biochemistry 2022Quote: ... The liposomes were homogenized by extruding 11 times through a 50 nm polycarbonate filter (Nuclepore Track-Etched Membranes, Whatman, Maidstone, UK) using a Mini Extruder instrument (Avanti Polar Lipids). QCM-D experiments were conducted with an X4 instrument equipped with a flow chamber (AWSensors ...
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bioRxiv - Biophysics 2023Quote: ... The lipid suspension containing multilamellar vesicles was extruded 21 times through a polycarbonate membrane with a 100 nm pore diameter using a mini-extruder from Avanti Polar Lipids.
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bioRxiv - Biochemistry 2023Quote: ... The vesicle suspension was further processed by five freeze-thawing cycles (90 s in liquid nitrogen followed by 90 s in a 60°C water bath) and extrusion (21 times) through a stack of polycarbonate membranes (pore size 200 nm) using a mini-extruder (Avanti Polar Lipids). The vesicles made from PC:PE were unstable (lipids felt out of solution after short term storage) ...
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bioRxiv - Microbiology 2023Quote: ... this solution was passed 21 times through an extrusion membrane (Whatman Nuclepore) with pore size of 2 μm on a mini-extruder (Avanti Polar Lipids) to get a clear solution comprising of LUVs ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting liposomes were extruded through a 50 nm pore diameter polycarbonate membrane (Cytiva Life Sciences) using a Mini-Extruder (Avanti Polar Lipids) with 23 passes.
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bioRxiv - Biophysics 2023Quote: ... and mixture was extruded through 100 nm pore membrane 11 times at 45 °C using the LiposoFast instrument (Avanti polar lipids, Inc.)
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bioRxiv - Microbiology 2022Quote: ... 20:1/20:1, and 22:1/22:1 (Özbalci et al., 2013)) and 5 pmol LPC (17:1, Avanti Polar Lipids). The PE P standard mix consisted of 16.5 pmol PE P-Mix 1 (16:0p/15:0 ...