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Citations for Avanti Polar Lipids :
851 - 894 of 894 citations for 4 1 tert butoxycarbonyl piperidin 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... A volume of 800 μL of chloroform:methanol (2:1 v/v) containing 15 μL internal standards (ISTDs, EquiSPLASH, Avanti Polar Lipids) were homogenized using the bead beating device (Precellys ...
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bioRxiv - Cell Biology 2021Quote: ... The final lipid extracts in chloroform:methanol (1:2, v:v) were spiked with internal phospholipid standards or Ceramide/Sphingoid Internal Standard Mixture I (Avanti Polar Lipids, USA) and 1% NH4OH (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of purified hZnT7 or hZnT7ΔHis-loop was added to 5 mg egg yolk phosphatidylcholine (egg PC, Avanti Polar Lipids) in 1 mL of PBS containing 0.8% sodium cholate (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Ceramidase activity was assessed by incubating 1.8 μM of purified SIDT1 and SIDT2 proteins with 40 μM ceramide (d18:1/18:0) (Cat# 860518P, Avanti Polar Lipids) at room temperature for 30 minutes ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: Hexadecanal (16:0 aldehyde) was purchased as 1 mg/ml (4.16 mM) in methylene chloride solution (Cat. No. 857458M, Avanti Polar Lipids), aliquoted into 25 μl ...
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bioRxiv - Immunology 2021Quote: ... Briefly, freshly isolated PBMCs (1 × 106 cell/ml, 5 ml/well) were treated with 100 ng/ml αGalCer (KRN7000, Avanti Polar Lipids) and cultured for 13 days ...
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bioRxiv - Biophysics 2021Quote: ... functional ChRmine at high density into phospholipid vesicles consisting of a 10:1 mixture of chicken egg yolk phosphatidylcholine (egg PC; Avanti Polar Lipids) and the adjuvant lipid A (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots were subjected to acidic Bligh and Dyer lipid extractions in the presence of internal lipid standards added from a master mix containing 40 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Biochemistry 2021Quote: ... A proteoliposome antigen was prepared by reconstituting purified functional OxlT at high density into phospholipid vesicles consisting of a 10:1 mixture of egg phosphatidylcholine (PC) (Avanti Polar Lipids) and adjuvant lipid A (Sigma ...
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bioRxiv - Physiology 2021Quote: ... 0.02-0.05 g of solid tissue or 150 μL of serum homogenized in 400μL 2:1 methanol:chloroform with 5nM/L PC 9:0-9:0 (Avanti Polar Lipids, Alabaster, Alabama) as an internal standard ...
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bioRxiv - Biochemistry 2022Quote: ... Aliquots corresponding to 1500-2000 pmol total lipid were subjected to acidic Bligh and Dyer extractions performed in the presence of internal lipid standards from a master mix containing 40 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Cell Biology 2022Quote: ... The used master mix of lipid internal standards contained 50 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was separated from cell debris and supplemented with NP-40 (EDM Biosciences) to a final concentration of 1% and DHPC (Avanti Polar lipids) to a final concentration of 30 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... a proteoliposome antigen was prepared by reconstituting purified hZnT7 at high density into phospholipid vesicles consisting of a 10:1 mixture of chicken egg yolk phosphatidylcholine (egg PC; Avanti Polar Lipids) and adjuvant lipid A (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... lipid mixtures were created by combining DSPC and DSPE-PEG2000 at a 9:1 molar ratio (Avanti Polar Lipids, Alabaster, AL, USA) in a solution that also included propylene glycol at a concentration of 15% (v/v) ...
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bioRxiv - Plant Biology 2024Quote: ... D-glucosyl-ß-1,1′-N-palmitoyl-D-erythro-sphingosine (d18:1/16:0 - GluCer or Glucosylceramide) were purchased from Avanti Polar Lipids. Sphingolipids were dissolved in ethanol (100%) ...
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bioRxiv - Cell Biology 2024Quote: ... Lipids were extracted by adding 990 µl of chloroform/methanol 17:1 (v/v) and internal standards (127 pmol ceramide 12:0 and 124 pmol sphingomyelin 12:0; Avanti Polar Lipids), followed by shaking at 900 rpm/min in a ThermoMixer (Eppendorf ...
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bioRxiv - Molecular Biology 2024Quote: ... Internal standard mixture (SPLASH® LIPIDOMIX® and Cer 18:1;O2/17:0, both from Avanti Polar Lipids, Alabaster, AL, USA) were added to the samples ...
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bioRxiv - Cell Biology 2023Quote: ... An equal volume of 2 μM Nitrobenzoxadiazole (NBD)-labeled lipids (Avanti Polar lipids: NBD-PC (18:1-06:0 NBD PC, 810132C); NBD-lyso-PC (12:0 lyso NBD PC ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL of snail serum was spiked with a known volume of SPLASH Lipidomix II mass spectrometry standard (Avanti Polar Lipids, #330709) and then extracted according to the method described above ...
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bioRxiv - Evolutionary Biology 2022Quote: ... lipid 1 in Table S1) was not commercially available during this project and therefore was synthesized de novo by Avanti Polar Lipids based on a previously reported procedure [60] with slight modifications starting with commercially available (R)-Solketal ...
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bioRxiv - Biochemistry 2023Quote: ... prior to denaturing intact protein mass spectrometry analysis the protein (2 mg mL-1) was incubated with 200 µM sphinganine (Avanti Polar Lipids), 200 µM fumonisin B1 (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... DMG-PEG (MW 2000) (DMG-PEG2000) (Cat. No. 880151P) and 18:1 PA (Cat. No. 840875P) were purchased from Avanti Polar Lipids. Cholesterol (Cat ...
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bioRxiv - Microbiology 2024Quote: ... the final lipid solution (20 mg ml-1) was extruded at least ten times through a Mini Extruder (Avanti Polar Lipids, USA) equipped with polycarbonate membranes of 0.1 μm pore size ...
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant (S2) was mixed with 1/8 volume of 10% NP-40 and 300 mM DHPC (reconstituted in lysis buffer; Avanti Polar Lipids 850306P) and incubated for 10 min on ice ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of 100 µM Palmitate-2,2-D2 (Eurisotop: DLM-1153-0)/ 1 x 10E6 cells For lipids (SPLASH LIPIDOMIX, Avanti Polar Lipids, 330707-1EA) 10 μl/sample) ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... 18:1 (Δ9-Cis) PC (DOPC) and 16:0 DNP Cap PE lipids were purchased from Avanti Polar Lipids (850375C and 810528C, respectively). DNP-Peg12-NHS ester was purchased from BroadPharm (BP-22397) ...
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bioRxiv - Microbiology 2023Quote: ... lyso sphingomyelin (LSM) d18:1 (d9) and palmitoyl-L-Carnitine (CAR) 16:0 (d3) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Arachidonic acid (AA ...
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bioRxiv - Biochemistry 2023Quote: ... for which 20 µL of mouse serum was combined with 180 µL of chloroform:methanol extraction solvent (v:v=1:2) containing internal standards at the final concentrations: 5nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5nM D3-Deoxysphinganine (Avanti Polar Lipids Inc. ...
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bioRxiv - Biophysics 2024Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (18:1 Liss Rhod PE) in chloroform form were purchased from Avanti polar lipids (USA). The A375 cell line was purchased from ATCC (USA).
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bioRxiv - Biophysics 2020Quote: ... as well as the in the sn-1 chain per-deuterated versions POPC-d31 and POPG-d31 were purchased as powder from Avanti Polar Lipids (Alabaster, AL), Cholesterol (Chol ...
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bioRxiv - Neuroscience 2024Quote: ... Each cell pellet was resuspended in 500 µL of resuspension buffer (0.05 M HCl, 49% Methanol, 1% SPLASH Lipidomic Mass Spec Standard [Avanti Polar Lipids, Cat. Nu. 330707]), and 1 mL methyl-tert-butyl-ether was added ...
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bioRxiv - Pathology 2023Quote: ... exact mass peaks corresponding to H+ adducts from standards of C18:1 (Δ-cis) carnitine oleoyl L-carnitine (Avanti Polar Lipids, Inc., Alabaster, AL) and 16:0-18:1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (Avanti Polar Lipids ...
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bioRxiv - Immunology 2023Quote: ... PE-D31 16:0/18:1 and TG-D5 19:0/12:0/19:0 (Product # 860374, 850367, 8609040 and 830456 from Avanti Polar lipids, Alabama, USA) were added ...
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bioRxiv - Neuroscience 2023Quote: ... and Acyl-CoA (18:0, 870718P, Avanti Polar Lipids Inc.; 16:0, 870716P, Avanti Polar Lipids Inc.; 24:1, 870725P, Avanti Polar Lipids Inc.) for reaction were water-bath sonicated prior to the reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of a customised isotope labelled internal standard lipid mixture and 1 μL of a d5-TG Internal Standard Mixture I (Avanti Polar Lipids, Alabaster, AL, USA) were added ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Fifty microlitres of each sample was combined with 100 μL ceramide 17:0 (1 μM in MeOH) and 25 μL dihydroceramide 12:0 (2 μM in MeOH) (Avanti Polar Lipids, Alabaster, AL, USA) as internal standards ...
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bioRxiv - Biophysics 2023Quote: ... Small unilamellar vesicles are prepared by drying a mixture of 93% POPC and 7% 18:1 biotinyl-PE (Avanti Polar Lipids, catalog #850457C and 870282C) under compressed nitrogen gas followed by overnight drying under vacuum ...
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bioRxiv - Cell Biology 2023Quote: ... and irreversibly bound fractions were each suspended in 300 µL of 1 M KOH in 95% CH3OH containing 2 µL of 1 µM N-(2’-(R)-hydroxypalmitoyl(d9)) D-erythro-sphingosine (Avanti Polar Lipids, Alabaster, AL, USA) as an internal standard and incubated at 60 °C for 1 h to release ω-OH ceramides from protein-bound ceramides ...
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Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... 20 μg of the soluble or membrane proteomic fraction obtained from mammalian tissues or cells was incubated with 100 μM lyso-PS substrate (C17:1 lyso-PS; Avanti Polar Lipids, catalog no: 858141) in DPBS to a final volume of 100 μL at 37 °C with constant shaking (Eppendorf ...
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bioRxiv - Physiology 2024Quote: ... The homogenate was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Physiology 2024Quote: ... The homogenate was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Physiology 2024Quote: ... Plasma was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Molecular Biology 2022Quote: ... USA) was added to all test samples before lipid extraction (10 μL of a 1:10 dilution of Avanti polar lipids to 10 μL of neat plasma) for normalisation of raw peak areas and to correct for differences in extraction(11).