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Citations for Avanti Polar Lipids :
801 - 850 of 867 citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... including 18:1 Cy5 Cardiolipin (1,1’,2,2’-tetraoleoyl cardiolipin-N-(Cyanine 5)) were purchased from Avanti Polar Lipids and prepared as previously described.19,30 Charge-reducing reagents ...
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bioRxiv - Microbiology 2021Quote: ... by extrusion using a Mini Extruder fitted with a polycarbonate membrane with 1 µm pore size (Avanti Polar Lipids) in binding buffer without magnesium (50 mM HEPES/KOH ...
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bioRxiv - Systems Biology 2020Quote: ... and 50 μL of the internal standard C12 ceramide (d18:1/12:0) (Avanti Polar Lipids, Alabaster, AL, USA) to samples with continuous shaking overnight (12 hours ...
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bioRxiv - Biochemistry 2021Quote: ... The microsomes were solubilized with 2% (w/v) CHAPS (Dojindo) and 1% (w/v) soybean lecithin (Avanti Polar Lipids) in 1 M NaCl ...
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bioRxiv - Neuroscience 2023Quote: A concentration of either 1 mg/mL Total Ganglioside Extract or 1mg/mL POPS was used (Avanti Polar Lipids). In brief ...
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bioRxiv - Cell Biology 2024Quote: A 50 μL methanol solution containing 20 pmol each of C15:0/18:1 PA (Avanti Polar Lipids, #330721) and C12:0/13:0 PE (Avanti Polar Lipids ...
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bioRxiv - Microbiology 2020Quote: ... Total lipid was then separated by 2D-HPTLC spiked with 1 μg PA(C17:0/C17:0) (Avanti Polar lipids) using chloroform/methanol/28% NH4OH ...
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bioRxiv - Biophysics 2020Quote: ... functional MgtE at high density in phospholipid vesicles consisting of a 10:1 mixture of egg phosphatidylcholine (Avanti Polar Lipids) and the adjuvant lipid A (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... Cer d18:1/C17:0 [4.5 ng/μl], GluCer d18:1/C12:0 [8.3 ng/μl] and GM1 [87 ng/μl], Avanti Polar Lipids) was added ...
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bioRxiv - Biochemistry 2020Quote: ... All lipid internal standards used are listed in Table 1 internal standards (all from Avanti Polar Lipids, Alabaster, AL, USA).
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bioRxiv - Immunology 2024Quote: ... 4 µL of a 1 mg/mL suspension of unilamellar vesicles made from DOPC (Avanti Polar Lipids, Alabaster, AL, USA) were deposited on freshly cleaved mica (∼10 mm diameter ...
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bioRxiv - Neuroscience 2024Quote: Mature microglia were exposed to 10 μM GalCer (C8 Galactosyl(β) Ceramide (d18:1/8:0)) (Avanti Polar Lipids, 860538P) for 16 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5 mol% PE-DGS-NTA(Ni2+) and 1 mol% LissRhodamine B-PE or Atto488-PE (all Avanti Polar Lipids, USA) were produced by extrusion ...
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bioRxiv - Biochemistry 2024Quote: A 2 mM stock solution of DHE in absolute ethanol was prepared from 1 mg solid (Avanti Polar Lipids, #810253). Dansyl-PE (1 mL ...
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bioRxiv - Biochemistry 2024Quote: Liposomes were prepared from a defined lipid mixture (egg PC:egg PE:wheat germ PI, 2:2:1 mol%) using a mini-extruder (Avanti Polar Lipids) as described previously (43) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (18:1 NBD PE) were purchased from Avanti Polar Lipids as powder and dissolved in chloroform to a final concentration of 100mg/ml (DOPC & DOPG ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was extruded through polycarbonate membranes with 1 or 0.2 µm pore size using a mini-extruder (Avanti Polar Lipids) to create large or small unilamellar vesicles (LUV/SUV) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide fractions were pooled then incubated at a molar ratio of 1:1.1 (excess lipid) with MPB-PE (Avanti Polar Lipids) for 3- 12 hours at 25C with end-over-end mixing ...
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bioRxiv - Cell Biology 2022Quote: ... and 5% 18:1 (0.33 mM final) PI phosphates (PI(3,5)P2 or PI(4,5)P2) (Avanti Polar lipids, Alabaster, AL) were prepared according to (Banerjee and Kane ...
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bioRxiv - Physiology 2021Quote: ... A volume of 800 μL of chloroform:methanol (2:1 v/v) containing 15 μL internal standards (ISTDs, EquiSPLASH, Avanti Polar Lipids) were homogenized using the bead beating device (Precellys ...
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bioRxiv - Cell Biology 2021Quote: ... The final lipid extracts in chloroform:methanol (1:2, v:v) were spiked with internal phospholipid standards or Ceramide/Sphingoid Internal Standard Mixture I (Avanti Polar Lipids, USA) and 1% NH4OH (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of purified hZnT7 or hZnT7ΔHis-loop was added to 5 mg egg yolk phosphatidylcholine (egg PC, Avanti Polar Lipids) in 1 mL of PBS containing 0.8% sodium cholate (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Ceramidase activity was assessed by incubating 1.8 μM of purified SIDT1 and SIDT2 proteins with 40 μM ceramide (d18:1/18:0) (Cat# 860518P, Avanti Polar Lipids) at room temperature for 30 minutes ...
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Transmembrane domains of H1 and H3 hemagglutinin contribute to membrane fusion in a different mannerbioRxiv - Biophysics 2024Quote: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) 18:1 (C6 NBD PE) used in FLIM was supplied from Avanti Polar Lipids. Acrylamide and Triton X-100 were from Sigma.
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: Hexadecanal (16:0 aldehyde) was purchased as 1 mg/ml (4.16 mM) in methylene chloride solution (Cat. No. 857458M, Avanti Polar Lipids), aliquoted into 25 μl ...
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bioRxiv - Immunology 2021Quote: ... Briefly, freshly isolated PBMCs (1 × 106 cell/ml, 5 ml/well) were treated with 100 ng/ml αGalCer (KRN7000, Avanti Polar Lipids) and cultured for 13 days ...
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bioRxiv - Biophysics 2020Quote: ... 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoinositol (PI(18:0/20:4)) and 1-stearoyl-2-linoleoyl-sn-glycerol (DG(18:0/18:2)) were purchased from Avanti Polar Lipids.
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bioRxiv - Biophysics 2021Quote: ... functional ChRmine at high density into phospholipid vesicles consisting of a 10:1 mixture of chicken egg yolk phosphatidylcholine (egg PC; Avanti Polar Lipids) and the adjuvant lipid A (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots were subjected to acidic Bligh and Dyer lipid extractions in the presence of internal lipid standards added from a master mix containing 40 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Biochemistry 2021Quote: ... A proteoliposome antigen was prepared by reconstituting purified functional OxlT at high density into phospholipid vesicles consisting of a 10:1 mixture of egg phosphatidylcholine (PC) (Avanti Polar Lipids) and adjuvant lipid A (Sigma ...
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bioRxiv - Physiology 2021Quote: ... 0.02-0.05 g of solid tissue or 150 μL of serum homogenized in 400μL 2:1 methanol:chloroform with 5nM/L PC 9:0-9:0 (Avanti Polar Lipids, Alabaster, Alabama) as an internal standard ...
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bioRxiv - Biochemistry 2022Quote: ... Aliquots corresponding to 1500-2000 pmol total lipid were subjected to acidic Bligh and Dyer extractions performed in the presence of internal lipid standards from a master mix containing 40 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Cell Biology 2022Quote: ... The used master mix of lipid internal standards contained 50 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was separated from cell debris and supplemented with NP-40 (EDM Biosciences) to a final concentration of 1% and DHPC (Avanti Polar lipids) to a final concentration of 30 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... a proteoliposome antigen was prepared by reconstituting purified hZnT7 at high density into phospholipid vesicles consisting of a 10:1 mixture of chicken egg yolk phosphatidylcholine (egg PC; Avanti Polar Lipids) and adjuvant lipid A (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... lipid mixtures were created by combining DSPC and DSPE-PEG2000 at a 9:1 molar ratio (Avanti Polar Lipids, Alabaster, AL, USA) in a solution that also included propylene glycol at a concentration of 15% (v/v) ...
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bioRxiv - Plant Biology 2024Quote: ... D-glucosyl-ß-1,1′-N-palmitoyl-D-erythro-sphingosine (d18:1/16:0 - GluCer or Glucosylceramide) were purchased from Avanti Polar Lipids. Sphingolipids were dissolved in ethanol (100%) ...
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bioRxiv - Cell Biology 2024Quote: ... Lipids were extracted by adding 990 µl of chloroform/methanol 17:1 (v/v) and internal standards (127 pmol ceramide 12:0 and 124 pmol sphingomyelin 12:0; Avanti Polar Lipids), followed by shaking at 900 rpm/min in a ThermoMixer (Eppendorf ...
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bioRxiv - Molecular Biology 2024Quote: ... Internal standard mixture (SPLASH® LIPIDOMIX® and Cer 18:1;O2/17:0, both from Avanti Polar Lipids, Alabaster, AL, USA) were added to the samples ...
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bioRxiv - Cell Biology 2023Quote: ... An equal volume of 2 μM Nitrobenzoxadiazole (NBD)-labeled lipids (Avanti Polar lipids: NBD-PC (18:1-06:0 NBD PC, 810132C); NBD-lyso-PC (12:0 lyso NBD PC ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL of snail serum was spiked with a known volume of SPLASH Lipidomix II mass spectrometry standard (Avanti Polar Lipids, #330709) and then extracted according to the method described above ...
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bioRxiv - Evolutionary Biology 2022Quote: ... lipid 1 in Table S1) was not commercially available during this project and therefore was synthesized de novo by Avanti Polar Lipids based on a previously reported procedure [60] with slight modifications starting with commercially available (R)-Solketal ...
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bioRxiv - Biochemistry 2023Quote: ... prior to denaturing intact protein mass spectrometry analysis the protein (2 mg mL-1) was incubated with 200 µM sphinganine (Avanti Polar Lipids), 200 µM fumonisin B1 (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... DMG-PEG (MW 2000) (DMG-PEG2000) (Cat. No. 880151P) and 18:1 PA (Cat. No. 840875P) were purchased from Avanti Polar Lipids. Cholesterol (Cat ...
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bioRxiv - Microbiology 2024Quote: ... the final lipid solution (20 mg ml-1) was extruded at least ten times through a Mini Extruder (Avanti Polar Lipids, USA) equipped with polycarbonate membranes of 0.1 μm pore size ...
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bioRxiv - Bioengineering 2024Quote: ... and 6-((2-hexyldecanoyl)oxy)-N-(6-((2-hexyldecanoyl)oxy)hexyl)-N-(4-hydroxybutyl)hexan-1-aminium (ALC-0315)were purchased from Avanti Polar Lipids, Inc ...
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant (S2) was mixed with 1/8 volume of 10% NP-40 and 300 mM DHPC (reconstituted in lysis buffer; Avanti Polar Lipids 850306P) and incubated for 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... or phosphatidylcholine and phosphatidylserine (PS) in a 4:1 molar ratio (PC:PS) were prepared by extrusion using 100 nm polycarbonate membranes (Avanti Polar Lipids, Alabaster, AL), kept a 4°C and used within 7 days ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of 100 µM Palmitate-2,2-D2 (Eurisotop: DLM-1153-0)/ 1 x 10E6 cells For lipids (SPLASH LIPIDOMIX, Avanti Polar Lipids, 330707-1EA) 10 μl/sample) ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... 18:1 (Δ9-Cis) PC (DOPC) and 16:0 DNP Cap PE lipids were purchased from Avanti Polar Lipids (850375C and 810528C, respectively). DNP-Peg12-NHS ester was purchased from BroadPharm (BP-22397) ...