Labshake search
Citations for Covaris :
1 - 50 of 67 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then washed three times in PBS and chromatin was isolated and sheared to 200-700 bp using the truChIP Chromatin Shearing Kit (Covaris, 520154) and a Covaris E220evolution focused-ultrasonicator following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were purified and fragmented using a sonicator (Covaris), and libraries were prepared according to the manufacturer’s instructions (NEBNext Ultra II DNA Library Prep ...
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bioRxiv - Cancer Biology 2023Quote: PCR products were fragmented using the Covaris M220 system (Covaris) to generate 3′-end cDNA fragments ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 times 30 seconds ON/OFF sonication was carried out at high intensity (Covaris S2 Focus Ultrasonicator) to disrupt the chromatin and release nuclear proteins ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed two times with PBS and then sonicated for 40 min (S220 focused- ultrasonicator, Covaris, Woburn, MA). After removal of cell debris ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (120 ng/µl) was sheared two times (back and forth) with a gTUBE (Covaris; P/N 520079) in a Eppendorf™ Centrifuge 5424 at 4800 rpm (soft ...
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bioRxiv - Plant Biology 2023Quote: ... and chromatin sonicated using the Diagenode Bioruptor (set to high intensity, 3 times 7 cyles (30sec ON / 30 sec OFF) or the S220 Focused-ultrasonicator (Covaris) for 20 min at peak power 110 W ...
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bioRxiv - Genomics 2020Quote: ... PCR products were then sheared by ultra-sonication (Covaris LE220-plus series, MA, USA) and prepared for sequencing using the SureSelectXT Library Preparation kit (Agilent ...
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bioRxiv - Genetics 2019Quote: ... One µg of DNA from each was fragmented to 250-bp fragments by adjusting the treatment time to 85 sec using a Covaris S220 sonicator (Covaris Inc.). Libraries were prepared using a Nextera DNA Sample Prep Kit and Bioo Scientific NEXTflex DNA Barcodes ...
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bioRxiv - Microbiology 2020Quote: ... One hundred nanograms of gDNA was sheared to <300-500> bp in size using a Covaris S220 ultrasonicator (shearing conditions: duty Cycle 10%, Cycles/burst 200 and treatment time 55 sec) (Covaris, MA). The size and the quality of the resulting DNA fragments were analyzed with an Agilent DNA High Sensitivity chip ...
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bioRxiv - Molecular Biology 2023Quote: ... The gRNA was fragmented into approximately 200 nt long fragments using an S220 Focused-ultrasonicator (Duty Cycle 10%; Intensity 5; Peak Incident Power 175 Watts; Cycles per Burst 200; Processing time 200 s; Covaris, Woburn, MA, USA). The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA samples were diluted to 75 ng in 15 µl of PCR certified water for subsequent shearing by sonication using the LE220-plus Focused-Ultrasonicator (Covaris) for 120 s at RT (duty factor 30% ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA samples were diluted to 75 ng in 15 µl of PCR certified water and sheared by sonication with a target of 200–250bp using the LE220-plus Focused-Ultrasonicator (Covaris) with the following settings ...
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bioRxiv - Genetics 2020Quote: The TruChIP tissue shearing kit (Covaris, Woburn, MA) was used to process 80 mg of mouse liver per sample ...
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bioRxiv - Genomics 2022Quote: ... and the Covaris truChIP Chromatin shearing kit (Covaris; 520154), according to the manufacturers’ instructions ...
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bioRxiv - Genomics 2021Quote: ... we used truChIP Chromatin Shearing Kit (Covaris Inc., MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Chromatin was prepared using truChIP Chromatin Shearing Tissue Kit (Covaris) according to the manufacture’s protocol with modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were prepared using truChIP Chromatin Shearing Reagent Kit (Covaris).
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bioRxiv - Genomics 2020Quote: A minimum of 200-800ng of the pooled PCR products in 55μl were then sheared by ultra-sonication (Covaris LE220-plus series, MA, USA) to generate a target fragment size of 250-750bp using the following parameters ...
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bioRxiv - Genomics 2020Quote: ... Chromatin shearing was performed using truChIP Chromatin Shearing Reagent Kit (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Using the truChIP High Cell Chromatin Shearing kit (Covaris, United States), cells were prepared for sonication according to the Covaris protocol ...
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bioRxiv - Genomics 2020Quote: ... Shearing of chromatin was performed using truChIP Chromatin Shearing Reagent Kit (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin was prepared with truChIP™ Chromatin Shearing Reagent Kit (Covaris 520154) and sheared with Covaris S220 according to the kit manual ...
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bioRxiv - Cancer Biology 2021Quote: ... Shearing of chromatin was performed using truChIP Chromatin Shearing Reagent Kit (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Shearing of chromatin was performed using truChIP Chromatin Shearing Reagent Kit (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Chromatin sonication was performed by using the truChIP Chromatin Shearing Kit (Covaris) with Focused-Ultrasonicator M220 (Corvaris ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were fixed and harvested using the truChIP chromatin shearing kit (Covaris) and IP was conducted according to Richard Myers lab ChIP-seq protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and for the clinical samples the truXTRAC FFPE kit was used (Covaris). DNase treatment was performed using the Turbo DNase kit (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... and processed for ChIP using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Chromatin was prepared with the truChIP™ Chromatin Shearing Kit (Covaris, Brighton, UK) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... for 15min and prepared for sonication with the truChIP Chromatin Shearing Kit (Covaris), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting cDNA was purified and fragmented using a Covaris fragmentation kit (Covaris, Inc.), profiled using an Agilent Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... for 15 min and prepared for sonication with the truChIP Chromatin Shearing Kit (Covaris), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin for ChIP was prepared and sheared using the truChIP Chromatin Shearing Kit (Covaris). Cells (∼5 million ESCs or iNs per biological replicate ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatin shearing was performed using the truChIP Chromatin Shearing kit (Covaris, cat. no. 520154) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cells were fixed in 1% formaldehyde and sonicated using the truChIP Chromatin Shearing Kit (Covaris) on the ME220 Focused-Ultrasonicator (Covaris ...
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bioRxiv - Cancer Biology 2022Quote: ... with RNAse treatment and from the FFPE sample with a turXTRAC FFPE DNA kit (Covaris). Library construction and sequencing were performed at the Beijing Genomics Institute (BGI) ...
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bioRxiv - Genetics 2021Quote: Chromatin preparation and shearing was performed using a Covaris truCHIP chromatin shearing tissue kit (Covaris 520237). Quenching buffer and fixing buffer were made following manufacturer’s instructions (Covaris 520237) ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were then prepared for sonication using the truChIP chromatin shearing kit (Covaris Cat# 520154). Briefly ...
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bioRxiv - Genomics 2019Quote: Sheared fixed chromatin was prepared exactly as described in the truChIP(tm) Chromatin Shearing Kit (Covaris) using 2 × 106 macrophage cells per AFA tube ...
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bioRxiv - Systems Biology 2023Quote: FFPE curls were treated with the truXTRAC FFPE total NA kit from Covaris (Key Resources Table) to extract DNA prior to library preparation ...
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bioRxiv - Cell Biology 2020Quote: ... hESCs were fixed with methanol-free formaldehyde provided by truChIP™ Chromatin Shearing Kit (Covaris, PN 520154). Chromatin of cell lysis was sheared using truChIP™ Chromatin Shearing Kit according its manual by ME220 Focused-ultrasonicator (Covaris) ...
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bioRxiv - Cell Biology 2020Quote: ... hESCs were fixed with methanol-free formaldehyde provided by truChIP™ Chromatin Shearing Kit (Covaris, PN 520154). Sonication of cell lysis was done by a ME220 Focused-ultrasonicator using truChIP Chromatin Shearing Tissue Kit (Covaris) ...
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bioRxiv - Cell Biology 2020Quote: ... Sonication of cell lysis was done by a ME220 Focused-ultrasonicator using truChIP Chromatin Shearing Tissue Kit (Covaris). Chromatin immunoprecipitation (ChIP ...
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bioRxiv - Developmental Biology 2022Quote: 50 AC explants per experimental condition were processed using the truCHIP Chromatin Shearing Tissue Kit with Formaldehyde (Covaris). Caps were fixed with 1% methanol-free formaldehyde-PBS (Covaris ...
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bioRxiv - Genomics 2021Quote: Chromatin was extracted and sheared from ~120 mg human hippocampus using truChIP Chromatin Shearing Kit (Covaris Inc., MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... ChIP was carried out in undifferentiated hESCs according to manuals of truChIP™ Chromatin Shearing Kit (Covaris, PN 520154) and EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... ChIP was carried out in undifferentiated hESCs according to manuals of truChIP™ Chromatin Shearing Kit (Covaris, PN 520154) and EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore ...