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Citations for Covaris :
1 - 50 of 52 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... microTUBE-15 beads strips (520159, Covaris) were used in a total volume of 15 µl ...
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bioRxiv - Systems Biology 2023Quote: ... The IFNg-treated cells were sonicated in AFA-tube TPX strips (Covaris), using a Covaris LE220R-Plus for 300 s at 325 peak power with a duty factor of 50 % ...
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bioRxiv - Genomics 2020Quote: ... Samples were sheared on the Covaris® LE220plus using the 8 microTUBE strip V1 (PM# 520053; Covaris, Woburn, MA) with the base pair (bp ...
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bioRxiv - Genetics 2021Quote: ... Quenching buffer and fixing buffer were made following manufacturer’s instructions (Covaris 520237). Hippocampi (25-40mg ...
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bioRxiv - Developmental Biology 2020Quote: ... microtubes with TPP buffer (Covaris) and lysed using a Covaris S220 Focused-ultrasonicator instrument with 125 W power over 180 seconds at 10% max peak power ...
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bioRxiv - Neuroscience 2022Quote: ... microtubes with TPP buffer (Covaris) and lysed using a Covaris S220 Focused-ultrasonicator instrument with 125 W power over 180 seconds ...
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bioRxiv - Microbiology 2022Quote: ... before 175 µl B1 buffer (Covaris) were added ...
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bioRxiv - Genomics 2023Quote: ProteinA magnetic Dynabeads (40 μl per sample) were blocked in Chromatin IP buffer (Shearing Buffer D3 diluted with 2X Covaris truChIP IP Buffer and supplemented with 1X Protease Inhibitor Cocktail ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking was stopped by Quenching Buffer E (Covaris). After washing ...
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bioRxiv - Genetics 2021Quote: ... and shearing buffer were made following manufacturer’s instructions (Covaris 520237). Pulverized tissue was thawed on ice and 400ul of lysis buffer was added to each tissue bag to resuspended the tissue with pipetting ...
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bioRxiv - Genetics 2021Quote: ... ChIP dilution buffer was made following manufacturer’s instructions (Covaris 520237). 160ul of ChIP dilution buffer was added to new a 1.5 microcentrifuge tube ...
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bioRxiv - Genomics 2021Quote: ... Beads were then washed thrice with Shearing Buffer D3 (Covaris), once with High Salt Washing Buffer (50mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... After dilution with one volume of immunoprecipitation dilution buffer (Covaris), sonicated samples were pre-cleared with 3 µL/mL of protein G magnetic beads (Ademtech ...
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bioRxiv - Physiology 2021Quote: ... followed by second fixation with 1% formaldehyde in Fixing Buffer A (Covaris) for 5 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... the nuclear pellet was resuspended in cold 0.25% SDS Shearing Buffer (Covaris). Chromatin was sheared in 1 ml AFA milliTUBEs (Covaris ...
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bioRxiv - Genomics 2021Quote: ... The samples were sonicated in 1X TE buffer using the Covaris S2 (Covaris) with the standard protocol for 50μL and 200bp insert size.
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bioRxiv - Genomics 2020Quote: ... The pelleted cells were resuspended in 130 μl of the sonication buffer (Covaris, 10 mM Tris-HCl ...
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bioRxiv - Genomics 2021Quote: 100ng of gDNA was sonicated in 1X TE buffer using the Covaris S2 (Covaris) with the standard protocol for 50μL and 200bp insert size.
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bioRxiv - Genomics 2021Quote: ... cells were resuspended in nuclei lysis buffer and sonicated using an AFA sonicator (Covaris). Immunoprecipitation was performed using a H3K27ac antibody (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... chromatin was dissolved into ChIP dilution buffer and sonication was conducted by S2 sonicator (Covaris). Chromatin and antibody were mixed and rotated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... together with 110 µl Tissue Lysis Buffer and 10 µl proteinase K solution (both Covaris). The lysate was processed with a M220 Focused ultrasonicator (Covaris ...
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bioRxiv - Molecular Biology 2021Quote: ... the pellet was resuspended to 350 μl of 1 M buffer B and sonicated (Covaris S220 sonicator ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Cells were then lysed using lysis buffer solutions and sonicated with the M220 ultrasonicator (Covaris) in 1 mL tube with an AFA Fiber and the following conditions (cycles/burst = 200 ...
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bioRxiv - Molecular Biology 2020Quote: ... The epidermis was homogenised in 8 M urea buffer with an ultrasonicator (S220X, Covaris, Brighton, UK) with 175 W peak power for 8 min ...
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bioRxiv - Developmental Biology 2019Quote: ... in 1× fixing buffer for 5 min at room temperature according to the vendor’s manual (Covaris). Crosslinked cells were then resuspended in 1× shearing buffer and sonicated with Covaris E220 for 780 sec using the following settings ...
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 2 ml shearing buffer and sonicated using a Focused Ultrasonicator M220 (Covaris). Sonication was performed in two rounds using two different sonication conditions ...
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bioRxiv - Cell Biology 2023Quote: ... were resuspended in 1 mL of buffer A2 and aliquoted into 1.5 mL tubes (Covaris®). Cells were sonicated for 8 min (Power ...
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bioRxiv - Genomics 2020Quote: ... Lysed cells were then diluted in 900mL TE buffer and sonicated using focused beam ultrasound sonicator (COVARIS). Sonicated samples were repeated for extended periods of time (upto 1.5 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin fragmentation was subsequently performed in RIPA buffer for 30 min using the S220 Focused-ultrasonicator (Covaris) in the Freq sweeping mode ...
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bioRxiv - Genomics 2024Quote: ... setosum DNA in 120 μl elution buffer was transferred to a g-tube (Covaris Part No. 520079). The sample was then proceeded to 6 passes of centrifugation with 2,000 xg for 2 min ...
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bioRxiv - Genomics 2019Quote: ... 100 ng of DNA in 130 μL of elution buffer was sheared on a S2 focused ultrasonicator (Covaris) for 65 seconds using intensity = 4 ...
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bioRxiv - Microbiology 2019Quote: ... Crosslinked nuclei were then resuspended in Covaris shearing buffer (0.1% SDS, 10 mM Tris pH 8.1, 1 mM EDTA) and sonicated using an M220 ultrasonicator (Covaris) and the following conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... nuclei were resuspended in 1 mL of shearing buffer and transferred to a milliTube with AFA fiber (Covaris). Chromatin was sheared using a Covaris S220 for 5 min (peak power ...
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bioRxiv - Genetics 2019Quote: ... Cells were resuspended in 1 ml shearing buffer (10 mM Tris pH 8.0, 5 mM EDTA, 0.1% SDS) and sonicated (Covaris) with the following settings (Peak Incident Power = 100 W ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA (2□μg) was diluted in 200 μL of elution buffer and fragmented using g-TUBE (Covaris) by centrifuging at 2400×g for 2□min ...
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bioRxiv - Immunology 2021Quote: ... in immunoprecipitation buffer (0.1% Triton X-100, 0.1 M Tris-HCl pH 8, 0.5 mM EDTA, and 0.15 M NaCl in 1Å~ Covaris D3 buffer) at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... for histone marks and SDS shearing buffer (10 mM Tris pH8, EDTA 1mM, SDS 0.15% and protease inhibitors) for transcription factor and sonicated (Covaris settings ...
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bioRxiv - Genetics 2022Quote: ... Cell nuclei were resuspended in 2 mL 0.4% SDS shearing buffer for sonication with a Covaris S220X sonicator (Covaris) for 7 min ...
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bioRxiv - Bioengineering 2021Quote: ... All samples were stored in low TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8) and were fragmented by Covaris ultrasonicator to have a mean size of 150 bp except cfDNA ...
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bioRxiv - Immunology 2020Quote: ... cells were washed twice in ice-cold PBS and lysed using the enclosed lysis buffer and sonicated by Covaris S2 (intensity ...
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bioRxiv - Genomics 2022Quote: ... for histone marks and SDS shearing buffer (10 mM Tris pH8, EDTA 1mM, SDS 0.15% and protease inhibitors) for transcription factor and sonicated (Covaris settings ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was washed twice and aliquots of 3.7 × 104 cells suspended in 20 µL of 100 mM ammonium bicarbonate (MilliporeSigma) buffer were loaded into individual wells on three 96-well AFA-Tube TPX plates (COVARIS). Cells were sonicated and lysed for 5 minutes using the LE220-Plus focused-ultrasonicator (COVARIS ...
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bioRxiv - Cancer Biology 2021Quote: ... and the pellet was resuspended in 1 mL shearing buffer and transferred to a 1 mL AFA tube (Covaris #520130).
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bioRxiv - Genomics 2022Quote: ... Pellets were resuspended in 1 ml of fresh shearing buffer and transferred into a cold 1 ml AFA tube (Covaris). Sonication conditions were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Ductal organoid samples were then resuspended in 1X S-Trap lysis buffer and homogenized by sonication using a LE220-Plus focused ultrasonicator (Covaris) at 300 peak power ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... around 30 brains in 110µl lysis buffer in one microtube-130 AFA Fiber Pre-Slit Snap-Cap (Catalog #520045, Covaris, Inc. Woburn, MA) were sonicated on a Covaris ME220 sonicator (Catalog # 500295 ...
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bioRxiv - Neuroscience 2020Quote: ... Aggregates were then diluted to a concentration of 100 µg/mL with 1x HBSS buffer followed by three 10s pulses of sonication using a 1/8” probe equipped QS5 Sonicator (Covaris, Woburn, MA). For cell culture experiments ...
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bioRxiv - Genomics 2024Quote: ... a dilution of 5 µg HMW DNA in 120 µL elution buffer was transferred to a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation with 1,990 x g for 2 min ...
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bioRxiv - Genomics 2022Quote: ... 1 µg of gDNA in 50 µl 1X low-EDTA TE buffer was sheared with a Covaris E220 sonicator (Covaris, Inc., Woburn, MA) to an average of 200 base pairs ...
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bioRxiv - Neuroscience 2019Quote: ... and positive INTACT-isolated sample 400 ng of gDNA was brought up to 50 µl volume with 1X low-EDTA TE buffer and sheared with a Covaris E220 sonicator (Covaris, Inc., Woburn, MA) to an average 200 base pair size using the following settings ...