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Citations for Covaris :
51 - 75 of 75 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Cells were fixed in 1% formaldehyde and sonicated using the truChIP Chromatin Shearing Kit (Covaris) on the ME220 Focused-Ultrasonicator (Covaris ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were sonicated and lysed for 5 minutes using the LE220-Plus focused-ultrasonicator (COVARIS) using 350 power peak intensity (PIP) ...
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bioRxiv - Genetics 2020Quote: The isolated genomic DNAs (~200 ngs/25K cell) were sonicated into 150 bp fragments (Covaris) and the entire reaction product was mixed with 20 μL of Streptavidin magnetic beads (Invitrogen 65001 ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Cells were then lysed using lysis buffer solutions and sonicated with the M220 ultrasonicator (Covaris) in 1 mL tube with an AFA Fiber and the following conditions (cycles/burst = 200 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were then prepared for sonication using the truChIP chromatin shearing kit (Covaris Cat# 520154). Briefly ...
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bioRxiv - Genetics 2022Quote: ... Cells were de-frozen on ice and chromatin was prepared following the TruChIP protocol from Covaris, as described in (Cohen et al ...
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bioRxiv - Genetics 2022Quote: ... Nuclei were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). Sonicated chromatin was transferred to 1.5mL microcentrifuge tubes and Triton-X 100 was added (1% final v/v ...
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bioRxiv - Neuroscience 2021Quote: ... ILPFC samples were fixed in 1% formaldehyde and cross-linked cell lysates were sheared by Covaris in 1% SDS lysis buffer to generate chromatin fragments with an average length of 300bp by using Peak Power ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). Lysates were transferred to tubes and Triton X-100 was added (final 1% ...
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bioRxiv - Genomics 2020Quote: ... Lysed cells were then diluted in 900mL TE buffer and sonicated using focused beam ultrasound sonicator (COVARIS). Sonicated samples were repeated for extended periods of time (upto 1.5 hours ...
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bioRxiv - Genetics 2022Quote: ... The cells were incubated on ice for 10 minutes and sonicated with an S220 Focused-Ultrasonicator (Covaris), using the software SonoLab 7.2 with the following program 10 repeats of treatment with a Peak Power= 200 ...
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bioRxiv - Cell Biology 2020Quote: ... Sonication of cell lysis was done by a ME220 Focused-ultrasonicator using truChIP Chromatin Shearing Tissue Kit (Covaris). Chromatin immunoprecipitation (ChIP ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA from FACS-sorted cells was randomly sheared to 100-500 bp in a microtube (Covaris; 520045) using a Covaris S220 device (400 sec ...
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bioRxiv - Genomics 2021Quote: One microgram of genomic DNA from the lymphoblastoid Coriell-NA12878 cell line was sheared using g-Tube (Covaris) to obtain high molecular weight fragments ranging from 50kb to 160 kb ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed two times with PBS and then sonicated for 40 min (S220 focused- ultrasonicator, Covaris, Woburn, MA). After removal of cell debris ...
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bioRxiv - Immunology 2020Quote: ... cells were washed twice in ice-cold PBS and lysed using the enclosed lysis buffer and sonicated by Covaris S2 (intensity ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin of cell lysis was sheared using truChIP™ Chromatin Shearing Kit according its manual by ME220 Focused-ultrasonicator (Covaris). The sheared chromatin was then incubated with anti-ARID1A antibody and purified by using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore ...
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bioRxiv - Immunology 2021Quote: ... B cell cultures were harvested at d2 and chromatin extracts were prepared using the truChIP Chromatin Shearing Reagent Kit (Covaris). 100μg of sonicated DNA protein complexes were used for immunoprecipitations with anti-IRF5 (Abcam #ab21689) ...
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bioRxiv - Molecular Biology 2023Quote: About 20.106 of Hela S3 cells per sample were prepared for sonication following the True-ChIP chromatin shearing kit protocol for High Cell concentration from Covaris. Cells were cross-linked in 1% methanol-free formaldehyde during 5 min before cell lysis and nuclei preparation ...
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bioRxiv - Genomics 2023Quote: ... 2-3x107 cells were collected and immediately processed for fixation using reagents provided in the truChIP Chromatin Shearing Kit with Formaldehyde (Covaris). After one wash with PBS ...
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bioRxiv - Genomics 2022Quote: High-molecular-weight DNA was isolated from frozen cell pellets using a modified Gentra Puregene method and sheared using gTUBE (Covaris, Inc.) to 20 kb mode size ...
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bioRxiv - Genomics 2020Quote: ... the DNA from UVB-irradiated cells was sheared to an average length of 300 bp by sonication with a Covaris E220 sonicator (Covaris; Woburn, MA) under the following conditions ...
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bioRxiv - Genomics 2019Quote: ... and genomic DNA from GM12878 lymphoblast cell line (Coriell Institute) were first sheared to an average fragment size of 8 kb using g-tubes (Covaris Cat. 520079). The fragmented DNA was PCR amplified to generate unmethylated DNA using the first steps of low input ligation kit SQK-LWP001 (ONT) ...
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bioRxiv - Plant Biology 2023Quote: ... Three flow cells were loaded with libraries constructed from DNA sheared to 20 Kbp using a G-tube (Covaris, Woburn, Massachusetts, USA). Reads were deposited in the ENA under project number PRJEB37025 ...
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bioRxiv - Molecular Biology 2022Quote: Chromatin was extracted using truChIP Chromatin Shearing Kit with formaldehyde and prepared according to manufacturer’s Low Cell protocol (Covaris PN010179 Rev O, Dec 2020). 11.1% formaldehyde was made fresh from 16% concentrated formaldehyde ...