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Citations for Covaris :
101 - 145 of 145 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1 µg of gDNA was fragmented by nebulization in g-tubes (Covaris, Woburn, MA, USA) to approximately 600 bp and subjected to end repair ...
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bioRxiv - Genomics 2020Quote: ... Lysed cells were then diluted in 900mL TE buffer and sonicated using focused beam ultrasound sonicator (COVARIS). Sonicated samples were repeated for extended periods of time (upto 1.5 hours ...
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bioRxiv - Genetics 2022Quote: ... The cells were incubated on ice for 10 minutes and sonicated with an S220 Focused-Ultrasonicator (Covaris), using the software SonoLab 7.2 with the following program 10 repeats of treatment with a Peak Power= 200 ...
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bioRxiv - Developmental Biology 2019Quote: ... in 1× fixing buffer for 5 min at room temperature according to the vendor’s manual (Covaris). Crosslinked cells were then resuspended in 1× shearing buffer and sonicated with Covaris E220 for 780 sec using the following settings ...
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bioRxiv - Cell Biology 2023Quote: ... were resuspended in 1 mL of buffer A2 and aliquoted into 1.5 mL tubes (Covaris®). Cells were sonicated for 8 min (Power ...
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bioRxiv - Cell Biology 2020Quote: ... Sonication of cell lysis was done by a ME220 Focused-ultrasonicator using truChIP Chromatin Shearing Tissue Kit (Covaris). Chromatin immunoprecipitation (ChIP ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA from FACS-sorted cells was randomly sheared to 100-500 bp in a microtube (Covaris; 520045) using a Covaris S220 device (400 sec ...
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bioRxiv - Genomics 2021Quote: One microgram of genomic DNA from the lymphoblastoid Coriell-NA12878 cell line was sheared using g-Tube (Covaris) to obtain high molecular weight fragments ranging from 50kb to 160 kb ...
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bioRxiv - Cell Biology 2020Quote: ... and 1 μg DNA was sonicated to ~300-1300 bp fragments using the E220 Focused-ultrasonicator (Covaris). The sonicated DNA was purified with a PCR purification kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% Triton X-100 and 20 mM Tris-HCl) with sonication using the Covaris S220 instrument (Covaris). Lysates were cleared by centrifugation at 14,000 x g for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of genomic DNA was sheared into approximatively 10 kb fragments using g-tubes (Covaris, England). A Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed two times with PBS and then sonicated for 40 min (S220 focused- ultrasonicator, Covaris, Woburn, MA). After removal of cell debris ...
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bioRxiv - Immunology 2020Quote: ... cells were washed twice in ice-cold PBS and lysed using the enclosed lysis buffer and sonicated by Covaris S2 (intensity ...
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bioRxiv - Genomics 2020Quote: ... approximately 1 μg of genomic DNA was acoustically sheared to ∼200 bp using a M220 Focused-ultrasonicator (Covaris). Sheared DNA was end repaired ...
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bioRxiv - Microbiology 2019Quote: ... Crosslinked nuclei were then resuspended in Covaris shearing buffer (0.1% SDS, 10 mM Tris pH 8.1, 1 mM EDTA) and sonicated using an M220 ultrasonicator (Covaris) and the following conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... nuclei were resuspended in 1 mL of shearing buffer and transferred to a milliTube with AFA fiber (Covaris). Chromatin was sheared using a Covaris S220 for 5 min (peak power ...
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bioRxiv - Genetics 2019Quote: ... Cells were resuspended in 1 ml shearing buffer (10 mM Tris pH 8.0, 5 mM EDTA, 0.1% SDS) and sonicated (Covaris) with the following settings (Peak Incident Power = 100 W ...
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bioRxiv - Genomics 2021Quote: 200 ng of genomic DNA was spiked with 1% unmethylated Lambda gDNA and fragmented to 400 bp (Covaris S220 ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin of cell lysis was sheared using truChIP™ Chromatin Shearing Kit according its manual by ME220 Focused-ultrasonicator (Covaris). The sheared chromatin was then incubated with anti-ARID1A antibody and purified by using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore ...
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bioRxiv - Immunology 2021Quote: ... B cell cultures were harvested at d2 and chromatin extracts were prepared using the truChIP Chromatin Shearing Reagent Kit (Covaris). 100μg of sonicated DNA protein complexes were used for immunoprecipitations with anti-IRF5 (Abcam #ab21689) ...
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bioRxiv - Molecular Biology 2023Quote: About 20.106 of Hela S3 cells per sample were prepared for sonication following the True-ChIP chromatin shearing kit protocol for High Cell concentration from Covaris. Cells were cross-linked in 1% methanol-free formaldehyde during 5 min before cell lysis and nuclei preparation ...
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bioRxiv - Genomics 2023Quote: ... 2-3x107 cells were collected and immediately processed for fixation using reagents provided in the truChIP Chromatin Shearing Kit with Formaldehyde (Covaris). After one wash with PBS ...
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bioRxiv - Genomics 2022Quote: High-molecular-weight DNA was isolated from frozen cell pellets using a modified Gentra Puregene method and sheared using gTUBE (Covaris, Inc.) to 20 kb mode size ...
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bioRxiv - Developmental Biology 2019Quote: ... The suspension was transferred to a milliTUBE 1 ml AFA fiber and sonicated on a E220 focused ultrasonicator (Covaris) using the following settings ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg DNA from each genotype was fragmented to ~500 bp size using the Covaris S2 (Covaris, MA, USA). The fragmented DNA was purified using 0.6X AMPure® XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... the DNA from UVB-irradiated cells was sheared to an average length of 300 bp by sonication with a Covaris E220 sonicator (Covaris; Woburn, MA) under the following conditions ...
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bioRxiv - Genomics 2019Quote: ... and genomic DNA from GM12878 lymphoblast cell line (Coriell Institute) were first sheared to an average fragment size of 8 kb using g-tubes (Covaris Cat. 520079). The fragmented DNA was PCR amplified to generate unmethylated DNA using the first steps of low input ligation kit SQK-LWP001 (ONT) ...
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bioRxiv - Plant Biology 2023Quote: ... Three flow cells were loaded with libraries constructed from DNA sheared to 20 Kbp using a G-tube (Covaris, Woburn, Massachusetts, USA). Reads were deposited in the ENA under project number PRJEB37025 ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... 1 µg of DNA from the unedited iPSC line was fragmented to approximately 350 bp using Covaris shearing (E220, Covaris). DNA fragments were end repaired and A-tailed using Ultra II reagents ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was fragmented by sonication on an E220 Covaris focused sonication machine using 1 ml glass AFA tubes (Covaris 520135) and the following parameters ...
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bioRxiv - Systems Biology 2023Quote: ... Chromatin was fragmented by sonication on an E220 Covaris focused sonication machine using 1 ml glass AFA tubes (Covaris 520135) and the following parameters ...
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bioRxiv - Plant Biology 2023Quote: ... and 1% proteinase inhibitor cocktail) with 160 U/mL RNase inhibitor and the nuclei were disrupted by sonication (Covaris S200). The sonicated samples were centrifuged at 13,800g for 5 minutes at 4°C to pellet debris ...
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bioRxiv - Microbiology 2019Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro-TUBE according to manufacturer’s recommended settings (Covaris, Woburn, MA). Sheared DNA was purified with 1x SPRIselect beads according to manufacturer’s instructions (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro- TUBE according to the manufacturer’s recommended settings (Covaris, Woburn, MA). HIV-1 insertion site libraries were prepared and sequenced using methods described previously (44) ...
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bioRxiv - Cancer Biology 2023Quote: ... were harvested and crosslinked with 1% formaldehyde using the TruChIP chromatin shearing kit as per the manufacturer’s instructions (Covaris, Cat # 520154). Chromatin was sheared using Covaris M220 Focused-ultrasonicator resulting in ∼ 200 bp fragments and diluted 1:1 with Covaris IP dilution buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... SDS was added to the final concentration of 1% and released chromatin solution was then transferred to microtubes with AFA fiber (Covaris, 520045) and sonicated for 14 cycles of 130 second each with peak incident power 70 and duty factor 20 in a Covaris ME220 focused ultrasonicator ...
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bioRxiv - Molecular Biology 2022Quote: Chromatin was extracted using truChIP Chromatin Shearing Kit with formaldehyde and prepared according to manufacturer’s Low Cell protocol (Covaris PN010179 Rev O, Dec 2020). 11.1% formaldehyde was made fresh from 16% concentrated formaldehyde ...
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bioRxiv - Neuroscience 2020Quote: ... Aggregates were then diluted to a concentration of 100 µg/mL with 1x HBSS buffer followed by three 10s pulses of sonication using a 1/8” probe equipped QS5 Sonicator (Covaris, Woburn, MA). For cell culture experiments ...
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bioRxiv - Genomics 2019Quote: ... with the following changes: 1 μg of gDNA was sheared to 70-90bp fragments using the Covaris S220 (Covaris Inc., Woburn, MA) and 150bp fragment libraries were constructed according to standard SOLiD Fragment library protocols (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... 1 µg of gDNA in 50 µl 1X low-EDTA TE buffer was sheared with a Covaris E220 sonicator (Covaris, Inc., Woburn, MA) to an average of 200 base pairs ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were suspended in an optimized solvent extraction mixture of 9:1 (methanol: chloroform) as described elsewhere in detail75 and extracted with a focused ultrasound system (Covaris E220, Woburn, USA). The extraction solutions were evaporated to dryness for 4 hours in a vacuum concentrator and afterwards pellets resuspended with 45 µL in a 1 mM TSP containing deuterated phosphate buffer ...
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bioRxiv - Molecular Biology 2023Quote: Control genomic DNA and amplification products were ethanol-precipitated and ∼1 mg of DNA was sheared into ∼350-bp fragments using a Covaris M220 focused acoustic shearer (Covaris, Woburn, MA, USA) following the manufacturer protocol ...
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bioRxiv - Genomics 2021Quote: Illumina libraries were generated by the Norwegian Sequencing Centre using the following protocol: 1 µg of genomic DNA was sheared using 96 microTUBE-50 AFA Fiber plates (Covaris Inc., Woburn, MA, USA) on a Covaris E220 system (Covaris Inc. ...