Labshake search
Citations for Covaris :
151 - 200 of 213 citations for PCR Tubes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... for preparation of 15 kbp libraries 8 µg genomic DNA and 1.4 µg plasmid DNA was sheared using g-tubes™ from Covaris, Woburn ...
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bioRxiv - Plant Biology 2019Quote: ... 6 μg genomic DNA in a total of 150 μl nuclease free water was sheared into ~8 kb with g-TUBEs (520079, Covaris) by following manufacturer’s instruction.
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bioRxiv - Evolutionary Biology 2022Quote: ... little skate genomic DNA (6.5–9 µg in 150 µl volume) was transferred to a Covaris g-Tube (Covaris, #520079) and sheared in a tabletop centrifuge (Eppendorf ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA samples (3 μg) were sheared to an average size of 10 kb via G-tube (Covaris, Woburn, MA) before library preparation ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was washed twice and aliquots of 3.7 × 104 cells suspended in 20 µL of 100 mM ammonium bicarbonate (MilliporeSigma) buffer were loaded into individual wells on three 96-well AFA-Tube TPX plates (COVARIS). Cells were sonicated and lysed for 5 minutes using the LE220-Plus focused-ultrasonicator (COVARIS ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from young fresh leaves and then randomly sheared to fragments with an average size of 20 kb using g-TUBE (Covaris). The sheared DNA was used to construct the PacBio SMRT library following the standard SMRT bell construction protocol (PacBio) ...
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bioRxiv - Plant Biology 2021Quote: A HiFi library was prepared according to the manual “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” with an initial DNA fragmentation by g-Tubes (Covaris) and final library size binning into defined fractions by SageELF (Sage Science) ...
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bioRxiv - Microbiology 2020Quote: ... 15 amplification cycles were used and input DNA (mock community phage genomes) was sheared at 10kbp (using Covaris g-TUBE). Influence of initial shearing length was also tested at 15kbp with a DNA input of 80 ng/μl ...
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bioRxiv - Cancer Biology 2021Quote: ... and the pellet was resuspended in 1 mL shearing buffer and transferred to a 1 mL AFA tube (Covaris #520130).
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bioRxiv - Genomics 2020Quote: ... Nuclear lysates were quickly spun down for 10 seconds at 2000g and supernatant transferred to sonication tubes (Covaris; product 520130) for shearing ...
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bioRxiv - Molecular Biology 2020Quote: ... two micrograms of high molecular weight genomic DNA from each sample (n=12 per pool) was sheared using a g0-tube to ~10kb (Covaris). These sheared gDNA samples were then used as input for SMRTbell preparation using the Template Preparation Kit 1.0 ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was fragmented by sonication on an E220 Covaris focused sonication machine using 1 ml glass AFA tubes (Covaris 520135) and the following parameters ...
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bioRxiv - Genomics 2021Quote: ... a total of 50 μg genomic DNA was sheared to ~20 kb targeted size by using Covaris g-TUBEs (Covaris). The sheared genomic DNA was examined by Agilent 2100 Bioanalyzer DNA12000 Chip (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... Pellets were resuspended in 1 ml of fresh shearing buffer and transferred into a cold 1 ml AFA tube (Covaris). Sonication conditions were as follows ...
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bioRxiv - Genomics 2022Quote: ... genomic DNA was adjusted to 10 ng/μL in 150 μL volume and sheared to approximately 10 kb fragments using g-TUBES (Covaris) following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... post-RCA DNA was debranched and fragmented to 6-8 kb-sized fragments using the above-mentioned protocol with the modification of the g-TUBE (Covaris) being centrifuged at 13200 rpm (16363 RCF ...
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bioRxiv - Plant Biology 2022Quote: ... The genomic DNA was sheared into ~20 kb DNA fragments with a g-TUBE Prep Station (Covaris, Woburn, MA, USA) and a HiFi SMRTbell library was constructed with the SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Systems Biology 2023Quote: ... Chromatin was fragmented by sonication on an E220 Covaris focused sonication machine using 1 ml glass AFA tubes (Covaris 520135) and the following parameters ...
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bioRxiv - Genomics 2023Quote: The genomic DNA was adjusted to 10 ng/uL in 150 µL and sheared to approximately 10 kilobase fragments using g-TUBES (Covaris) following the manufacturer’s instructios. ...
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bioRxiv - Cancer Biology 2023Quote: ... material was adjusted to a final volume 100μL and sonicated in microTUBE AFA Fiber screw cap tubes (6 mm × 16 mm, Covaris, 520096) using the Covaris S220 Focused-ultrasonicator at a maximum power for 15 seconds ...
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bioRxiv - Plant Biology 2023Quote: ... Young leaves from a single 3-week-old plant were collected for high-molecular-weight DNA extraction followed by shearing to fragments with an average size of 20 kb using g-TUBE (Covaris). The sheared DNA was then used to construct a PacBio SMRT library following the standard SMRT bell construction protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PacBio-HiFi libraries were prepared according to the manual “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” with an initial DNA fragmentation by g-Tubes (Covaris) and final library size selection on BluePippin (Sage Science) ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA (1µg) was first fragmented using a Covaris M220 focused ultrasonicator in micro-TUBE 50 AFA Fiber screw cap (Covaris, 520166) for a target insert size of 200 bp under the following conditions ...
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bioRxiv - Genomics 2023Quote: ... The DNA was sheared to approximately 25 kb using a Covaris® G-tube (#520079, Covaris, LLC, Woburn, MA, USA). The sequencing library was prepared using the ligation sequencing DNA v14 method (SQK-LSK114 ...
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bioRxiv - Microbiology 2019Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro-TUBE according to manufacturer’s recommended settings (Covaris, Woburn, MA). Sheared DNA was purified with 1x SPRIselect beads according to manufacturer’s instructions (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro- TUBE according to the manufacturer’s recommended settings (Covaris, Woburn, MA). HIV-1 insertion site libraries were prepared and sequenced using methods described previously (44) ...
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bioRxiv - Microbiology 2021Quote: ... DNA was sheared to an average length of approximately 5-10 kb (genomic DNA) using g-TUBEs (Covaris, Woburn, MA, USA) and SMRTbell template sequencing libraries were prepared using sheared DNA ...
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bioRxiv - Genomics 2021Quote: 15-20 μg of gDNA was diluted in 300 μl with 1x EB and was sheared using g-TUBE (Covaris, 520079) at 3500 - 4600 rpm in the Eppendorf 5424 (Eppendorf ...
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bioRxiv - Microbiology 2023Quote: ... were subjected to soni cation using the Covaris S220 (Power = 140, Duty = 5, Cycles/Burst = 200) for 18 minutes with microTUBE 500 AFA tubes (Covaris 520185) and each sample was incubated with 5 µg anti-FLAG M2 antibody (Millipore A2220 ...
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bioRxiv - Genetics 2023Quote: ... The soluble fraction was isolated by centrifugation at 2,000 rpm for 3min then transferred to sonication tubes (Covaris milliTUBE 1ml, 520135) and samples were sonicated to produce sheared chromatin with a size range of 200-1,000bp using a Covaris sonicator ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Half of the DNA was then sheared into 20 kb fragments with a COVARIS g-TUBE (Covaris, Inc., Woburn, MA, USA), centrifuging at 4,200 rpm for 1 minute to increase DNA throughput ...
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bioRxiv - Genomics 2024Quote: 120 μl of DNA sample with around 8-10 μg DNA was transferred to a g-tube (Covaris Part No. 520079). The sample was then proceeded to 6 passes of centrifugation with 2,000 xg of 2 min ...
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bioRxiv - Genomics 2020Quote: ... The DNA was sheared to a mean fragment length of 20-30 kb with Covaris g-TUBEs (Covaris Inc., Woburn, MA, USA). The length of the DNA was measured with a TapeStation (4200 ...
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bioRxiv - Genomics 2020Quote: ... two micrograms of high molecular weight genomic DNA from each sample was sheared using g0-tube to ∼8 Kb (Covaris, Woburn, MA). These sheared gDNA samples were size selected to include 5 Kb-9 Kb fragments with Blue Pippin (Sage Science ...
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bioRxiv - Neuroscience 2020Quote: ... 50 mg of deep-frozen feces sample were transferred into 2 ml adaptive focused acoustics (AFA) glass tubes (Covaris Inc, Woburn, USA) and mixed with 400 μl of ultrapure methanol and 800 μL of Methyl ter-butyl ether (MTBE ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (5-10 µg) samples were sheared to an average size of 20 kbp via G-tube (Covaris; Woburn, MA, USA), end repaired and ligated to hairpin barcoded adapters prior to sequencing ...
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bioRxiv - Genomics 2019Quote: ... and genomic DNA from GM12878 lymphoblast cell line (Coriell Institute) were first sheared to an average fragment size of 8 kb using g-tubes (Covaris Cat. 520079). The fragmented DNA was PCR amplified to generate unmethylated DNA using the first steps of low input ligation kit SQK-LWP001 (ONT) ...
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bioRxiv - Genomics 2022Quote: ... DNA ligation products were sonicated using Covaris M220 focused-ultrasonicator (20% duty factor, 50 peak incident power, 200 cycles per burst, 65 seconds) in 130ul tubes (Covaris cat. #520077). After shearing ...
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bioRxiv - Genetics 2022Quote: ... Three microgram of HMW gDNA was sheared to an average size distribution of ~8 kb using the Covaris g-TUBE (Covaris, MA, U.S.A.) in an Eppendorf 5810 R centrifuge at 5,000 rpm for a total of 2 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Three flow cells were loaded with libraries constructed from DNA sheared to 20 Kbp using a G-tube (Covaris, Woburn, Massachusetts, USA). Reads were deposited in the ENA under project number PRJEB37025 ...
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bioRxiv - Genomics 2023Quote: ... The starting material for each region (2500mg for large regions and 500mg for small regions) was sealed inside of a Covaris Tissue TUBE (Covaris 520021, 520023) and thoroughly pulverized using the chilled hammer and metallic block ...
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bioRxiv - Genetics 2024Quote: ... of purified DNA were fragmented to ∼350bp using the Covaris S2 system (10% duty cycle, intensity 5 for 2x 45 sec in Covaris AFA tubes) followed by a concentration step with Zymo columns (DNA Clean & Concentrator) ...
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bioRxiv - Genomics 2024Quote: ... a dilution of 5 µg HMW DNA in 120 µL elution buffer was transferred to a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation with 1,990 x g for 2 min ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were purified and fragmented using a sonicator (Covaris), and libraries were prepared according to the manufacturer’s instructions (NEBNext Ultra II DNA Library Prep ...
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bioRxiv - Cancer Biology 2023Quote: PCR products were fragmented using the Covaris M220 system (Covaris) to generate 3′-end cDNA fragments ...
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bioRxiv - Genomics 2019Quote: ... we prepared the library using 10 μg genomic DNA that was sheared to a size of 20kb fragments by g-tubes (Covaris, Inc., Woburn, MA) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2021Quote: ... SMRTbell libraries were constructed from genomic DNA samples sheared to between 10 and 20 kb using the G-tubes protocol (Covaris; Woburn, MA, USA), end repaired ...
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bioRxiv - Pathology 2021Quote: Frozen tissue samples from heart and skeletal muscle were transferred in a prechilled tubes and cryo-pulverized in a Covaris CP02 Automated Dry Pulverizer (Covaris Inc, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... a SMRTbell library was constructed from a genomic DNA sample sheared to an average size of ~10 kb using the G-tubes protocol (Covaris, Woburn, MA, USA). Single strands were removed ...
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bioRxiv - Genomics 2021Quote: ... STK005 SMRTbell libraries were constructed from genomic DNA samples sheared to between 10 and 20 kb using the G-tubes protocol (Covaris; Woburn, MA, USA), end repaired ...