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Citations for Covaris :
51 - 84 of 84 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1 µg of gDNA was fragmented by nebulization in g-tubes (Covaris, Woburn, MA, USA) to approximately 600 bp and subjected to end repair ...
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bioRxiv - Developmental Biology 2019Quote: ... in 1× fixing buffer for 5 min at room temperature according to the vendor’s manual (Covaris). Crosslinked cells were then resuspended in 1× shearing buffer and sonicated with Covaris E220 for 780 sec using the following settings ...
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bioRxiv - Neuroscience 2021Quote: ... ILPFC samples were fixed in 1% formaldehyde and cross-linked cell lysates were sheared by Covaris in 1% SDS lysis buffer to generate chromatin fragments with an average length of 300bp by using Peak Power ...
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bioRxiv - Genomics 2022Quote: ... 1-2 micrograms of high molecular weight DNA was sheared using g-tube (Covaris, Woburn, MA) to 5-9 Kbp at a 7000 RPM and size selected using the 0.75% DF 3-10kb Marker S1-Improved Recovery cassette definition on the Blue Pippin (Sage Science) ...
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bioRxiv - Cell Biology 2023Quote: ... were resuspended in 1 mL of buffer A2 and aliquoted into 1.5 mL tubes (Covaris®). Cells were sonicated for 8 min (Power ...
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bioRxiv - Cell Biology 2020Quote: ... and 1 μg DNA was sonicated to ~300-1300 bp fragments using the E220 Focused-ultrasonicator (Covaris). The sonicated DNA was purified with a PCR purification kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% Triton X-100 and 20 mM Tris-HCl) with sonication using the Covaris S220 instrument (Covaris). Lysates were cleared by centrifugation at 14,000 x g for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of genomic DNA was sheared into approximatively 10 kb fragments using g-tubes (Covaris, England). A Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... approximately 1 μg of genomic DNA was acoustically sheared to ∼200 bp using a M220 Focused-ultrasonicator (Covaris). Sheared DNA was end repaired ...
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bioRxiv - Microbiology 2019Quote: ... Crosslinked nuclei were then resuspended in Covaris shearing buffer (0.1% SDS, 10 mM Tris pH 8.1, 1 mM EDTA) and sonicated using an M220 ultrasonicator (Covaris) and the following conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... nuclei were resuspended in 1 mL of shearing buffer and transferred to a milliTube with AFA fiber (Covaris). Chromatin was sheared using a Covaris S220 for 5 min (peak power ...
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bioRxiv - Genetics 2019Quote: ... Cells were resuspended in 1 ml shearing buffer (10 mM Tris pH 8.0, 5 mM EDTA, 0.1% SDS) and sonicated (Covaris) with the following settings (Peak Incident Power = 100 W ...
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bioRxiv - Genomics 2021Quote: 200 ng of genomic DNA was spiked with 1% unmethylated Lambda gDNA and fragmented to 400 bp (Covaris S220 ...
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bioRxiv - Neuroscience 2019Quote: ... Lysate from cells or tissue was fixed in 1% formaldehyde and cross-linked cell lysates were sheared by Covaris in 1% SDS lysis buffer to generate chromatin fragments with an average length of 300bp ...
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bioRxiv - Developmental Biology 2019Quote: ... The suspension was transferred to a milliTUBE 1 ml AFA fiber and sonicated on a E220 focused ultrasonicator (Covaris) using the following settings ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg DNA from each genotype was fragmented to ~500 bp size using the Covaris S2 (Covaris, MA, USA). The fragmented DNA was purified using 0.6X AMPure® XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... A total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... 1 µg of DNA from the unedited iPSC line was fragmented to approximately 350 bp using Covaris shearing (E220, Covaris). DNA fragments were end repaired and A-tailed using Ultra II reagents ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was fragmented by sonication on an E220 Covaris focused sonication machine using 1 ml glass AFA tubes (Covaris 520135) and the following parameters ...
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bioRxiv - Systems Biology 2023Quote: ... Chromatin was fragmented by sonication on an E220 Covaris focused sonication machine using 1 ml glass AFA tubes (Covaris 520135) and the following parameters ...
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bioRxiv - Plant Biology 2023Quote: ... and 1% proteinase inhibitor cocktail) with 160 U/mL RNase inhibitor and the nuclei were disrupted by sonication (Covaris S200). The sonicated samples were centrifuged at 13,800g for 5 minutes at 4°C to pellet debris ...
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bioRxiv - Microbiology 2019Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro-TUBE according to manufacturer’s recommended settings (Covaris, Woburn, MA). Sheared DNA was purified with 1x SPRIselect beads according to manufacturer’s instructions (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro- TUBE according to the manufacturer’s recommended settings (Covaris, Woburn, MA). HIV-1 insertion site libraries were prepared and sequenced using methods described previously (44) ...
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bioRxiv - Cancer Biology 2023Quote: ... were harvested and crosslinked with 1% formaldehyde using the TruChIP chromatin shearing kit as per the manufacturer’s instructions (Covaris, Cat # 520154). Chromatin was sheared using Covaris M220 Focused-ultrasonicator resulting in ∼ 200 bp fragments and diluted 1:1 with Covaris IP dilution buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... SDS was added to the final concentration of 1% and released chromatin solution was then transferred to microtubes with AFA fiber (Covaris, 520045) and sonicated for 14 cycles of 130 second each with peak incident power 70 and duty factor 20 in a Covaris ME220 focused ultrasonicator ...
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bioRxiv - Neuroscience 2020Quote: ... Aggregates were then diluted to a concentration of 100 µg/mL with 1x HBSS buffer followed by three 10s pulses of sonication using a 1/8” probe equipped QS5 Sonicator (Covaris, Woburn, MA). For cell culture experiments ...
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bioRxiv - Genomics 2019Quote: ... with the following changes: 1 μg of gDNA was sheared to 70-90bp fragments using the Covaris S220 (Covaris Inc., Woburn, MA) and 150bp fragment libraries were constructed according to standard SOLiD Fragment library protocols (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... For the paired end library a total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
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bioRxiv - Genomics 2022Quote: ... 1 µg of gDNA in 50 µl 1X low-EDTA TE buffer was sheared with a Covaris E220 sonicator (Covaris, Inc., Woburn, MA) to an average of 200 base pairs ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were suspended in an optimized solvent extraction mixture of 9:1 (methanol: chloroform) as described elsewhere in detail75 and extracted with a focused ultrasound system (Covaris E220, Woburn, USA). The extraction solutions were evaporated to dryness for 4 hours in a vacuum concentrator and afterwards pellets resuspended with 45 µL in a 1 mM TSP containing deuterated phosphate buffer ...
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bioRxiv - Molecular Biology 2023Quote: Control genomic DNA and amplification products were ethanol-precipitated and ∼1 mg of DNA was sheared into ∼350-bp fragments using a Covaris M220 focused acoustic shearer (Covaris, Woburn, MA, USA) following the manufacturer protocol ...
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bioRxiv - Genomics 2021Quote: Illumina libraries were generated by the Norwegian Sequencing Centre using the following protocol: 1 µg of genomic DNA was sheared using 96 microTUBE-50 AFA Fiber plates (Covaris Inc., Woburn, MA, USA) on a Covaris E220 system (Covaris Inc. ...