Labshake search
Citations for Covaris :
451 - 500 of 558 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... Hi-C template DNA was sheared to a size of 300-500 bp using microTUBE snap-caps (Covaris, 520045) in a Covaris M220 sonicator and MyOne Streptavidin T1 beads (Life Technologies ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... USA) with DNA fragmented to an approximate size of 300 bp with a Covaris M220 sonicator (Covaris,Woburn, USA). Libraries were dual-indexed ...
-
bioRxiv - Genomics 2023Quote: ... ∼2.5ug of DNA in a volume of 130 µL was sheared to ∼800bp using a S220 focused ultrasonicator (Covaris) and the following parameters ...
-
bioRxiv - Plant Biology 2022Quote: ... The isolated DNA was fragmented by sonication to 200-300 bp using a Covaris S220 (Covaris, Woburn, MA, USA), followed by end repair and A-ligation ...
-
bioRxiv - Plant Biology 2023Quote: ... HiFi libraries were then prepared according to the manual “Procedure & Checklist - Preparing HiFi SMRTbellR Libraries using SMRTbell Express Template Prep Kit 2.0” with an initial DNA fragmentation by g-Tubes (Covaris) and final library size selection on BluePippin (Sage Science) ...
-
bioRxiv - Genetics 2023Quote: ... The chromatin DNA was sheared into fragments of between 200 and 1,200 base pairs in length by sonication (Covaris). The chromatin DNA-protein complexes were incubated with an anti-MeCP2 antibody (Cell Signaling Technology ...
-
bioRxiv - Genomics 2024Quote: ... DNA was sonicated to a 100–800 bp size range using a Covaris E220 sonicator (Covaris, Woburn, MA, USA), end-repaired and 3′-adenylated ...
-
bioRxiv - Molecular Biology 2024Quote: ... The DNA was then sheared to 200-300 bp with a Covaris M220 focused ultrasonicator (130 uL tube [Covaris part number 520045] ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3µg of genomic DNA was diluted in RNase-Free water and transferred to a AFA Fiber Crimp-Cap (PN520052, Covaris) microtube ...
-
bioRxiv - Genomics 2020Quote: ... A total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
-
bioRxiv - Genomics 2020Quote: ... Eight μg of DNA was mechanically sheared to an average size of 20-30 kb using a g-Tube device (Covaris). The SMRTbell was produced using the SMRTbell Express Template Prep Kit ...
-
bioRxiv - Genetics 2020Quote: Purified human genomic DNA was sheared into ∼150-200 base-pair fragments using the S220 Focused-Ultrasonicator (Covaris, Woburn, Massachusetts) according to the instructions provided by the manufacturer ...
-
Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... 1 µg of DNA from the unedited iPSC line was fragmented to approximately 350 bp using Covaris shearing (E220, Covaris). DNA fragments were end repaired and A-tailed using Ultra II reagents ...
-
bioRxiv - Plant Biology 2019Quote: ... 6 μg genomic DNA in a total of 150 μl nuclease free water was sheared into ~8 kb with g-TUBEs (520079, Covaris) by following manufacturer’s instruction.
-
bioRxiv - Plant Biology 2020Quote: ... 3 μg of DNA was sheared to 350 bp average size using an S220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Barcoded ...
-
bioRxiv - Microbiology 2019Quote: ... For Illumina sequencing 1ug of genomic DNA was sheared into 300–500 base pair (bp) fragments by focused ultrasonication (Covaris Adaptive Focused Acoustics technology ...
-
bioRxiv - Genomics 2021Quote: Metagenomic libraries were prepared by shearing 100 ng of DNA to 550 bp long products using the Covaris LE220 (Covaris) and size selected with SPRI beads (Beckman Coulter) ...
-
bioRxiv - Genomics 2020Quote: We fragmented MALBAC amplified DNA (>1ng/μL, 50μL) using Covaris M220 Focused Ultrasonicator in microTUBE-50 AFA Fiber Screw-Cap (Covaris, USA) to a target size of 350bp using a treatment time of 150s ...
-
bioRxiv - Molecular Biology 2020Quote: ... The resulting double-stranded cDNA/DNA mix was sheared to an average fragment size of 200 bp using a M220 focused ultrasonicator (Covaris). Sheared product was purified using the ZR-96 DNA Clean & Concentrator-5 kit (Zymo) ...
-
bioRxiv - Genomics 2021Quote: ... and 0.5 ng of all-C hydroxymethylated pUC19 plasmid DNA (Wisegene) and sonicated to ∼300 bp fragments using a M220 focused ultrasonicator (Covaris), with the following parameters ...
-
bioRxiv - Microbiology 2021Quote: 40 ng of each plasmid DNA was sheared into approximately 350 base pair in length by sonication using a Covaris Sonicator LE220 (Covaris). Fragmented DNA was uniquely index tagged with NEBNext Multiplex Oligos for Illumina (New England Bio-Labs ...
-
bioRxiv - Cancer Biology 2021Quote: ... 150-300 ng of DNA was fragmented to approximately 200 bp using a focal acoustic device (Covaris S2, Sage Sciences). Libraries and hybridization capture were performed following the SureSelectXT HS recommended protocol with SureSelect Human All Exon V6+UTR baits (Agilent) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... little skate genomic DNA (6.5–9 µg in 150 µl volume) was transferred to a Covaris g-Tube (Covaris, #520079) and sheared in a tabletop centrifuge (Eppendorf ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA samples (3 μg) were sheared to an average size of 10 kb via G-tube (Covaris, Woburn, MA) before library preparation ...
-
bioRxiv - Microbiology 2022Quote: ... The DNA was then sheared to an average length of 400 bp using a Covaris E220 Focused-ultrasonicator (Covaris, Inc.) and fragments size-selected with AMPure XP beads to achieve a size distribution of 200-600 bp ...
-
bioRxiv - Microbiology 2020Quote: 800 ng of genomic DNA isolated from week 2 and week 5 samples was sonicated using an E220 focused ultrasonicator (Covaris) to produce 400 bp fragments which were purified using Agencourt AMPure XP beads (Beckman Coulter) ...
-
bioRxiv - Plant Biology 2021Quote: A HiFi library was prepared according to the manual “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” with an initial DNA fragmentation by g-Tubes (Covaris) and final library size binning into defined fractions by SageELF (Sage Science) ...
-
bioRxiv - Microbiology 2020Quote: ... 15 amplification cycles were used and input DNA (mock community phage genomes) was sheared at 10kbp (using Covaris g-TUBE). Influence of initial shearing length was also tested at 15kbp with a DNA input of 80 ng/μl ...
-
bioRxiv - Microbiology 2021Quote: ... Extracted DNA was diluted to 500 ng and sheared to 200-300 bp using an E220 focused ultrasonicator (Covaris, U.K.). NEBNext Ultra DNA Library prep kit for Illumina was used for adaptor ligation and to PCR enrichment following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... extracted DNA (see above) was fragmented to an average insert size of ∼350 bp using acoustic sonication (Covaris model S220). Adapter-ligated fragment libraries were generated using the Kapa Biosystems HyperPrep PCR-free library preparation workflow ...
-
bioRxiv - Genomics 2019Quote: ... DNA was then resuspended in 10 mM T ris-HCl pH 8 and 5 ug aliquots were sonicated by Covaris S2 to fragment sizes in the range of 450-550 bp ...
-
bioRxiv - Microbiology 2021Quote: 2 μg of extracted DNA libraries were resuspended in purified water and sheared to approximately 550 bp fragments using a S220 focussed-ultrasonicator (Covaris), according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... two micrograms of high molecular weight genomic DNA from each sample (n=12 per pool) was sheared using a g0-tube to ~10kb (Covaris). These sheared gDNA samples were then used as input for SMRTbell preparation using the Template Preparation Kit 1.0 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Agilent SureSelectXT Human All Exon V6+COSMIC or Agilent Human All Exon 50Mb library preparation protocols were used with inputs of 100-3000ng sheared genomic DNA (Covaris). Library construction was performed using the Agilent Technologies SureSelectXT Reagent Kit ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 2-3 µg of DNA was sheared to 400 bp or 800 bp using a Covaris LE220 pulse focused sonicator (Covaris) and size selected using a Pippin Prep (Sage Science) ...
-
bioRxiv - Genomics 2021Quote: ... A total amount of 0.4µg DNA per sample was fragmented to an average size of ∼350bp with hydrodynamic shearing system (Covaris, Massachusetts, USA) and subjected to DNA library preparation with Illumina TruSeq DNA sample preparation kit (15026486 ...
-
bioRxiv - Genomics 2021Quote: ... We generated the short-read data for the even-year genome by shearing 1ug of DNA (pink even-year male described above) with a COVARIS LE220 (Covaris) using the following configuration in a 96 microTUBE plate (Covaris) ...
-
bioRxiv - Genomics 2021Quote: ... a total of 50 μg genomic DNA was sheared to ~20 kb targeted size by using Covaris g-TUBEs (Covaris). The sheared genomic DNA was examined by Agilent 2100 Bioanalyzer DNA12000 Chip (Agilent Technologies ...
-
bioRxiv - Genetics 2020Quote: ... DNA sequencing libraries were prepared for sequencing on the Illumina HiSeq X by the HudsonAlpha Genomic Services Lab by Covaris shearing ...
-
bioRxiv - Cancer Biology 2020Quote: ... 200 ng of genomic DNA was fragmented to 150-200 bp pairs using ultrasonication on the LE220 Focused-ultrasonicator (Covaris). Library preparation was performed using the SureSelect XT Library Prep Kit (Agilent Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... 250 ng DNA was sonicated to a 100–1,000 bp size range using the E220 Covaris Focused-Ultrasonicator (Covaris, Inc.). The fragments were end-repaired ...
-
bioRxiv - Microbiology 2022Quote: ... ∼2 μg of genomic DNA was fragmented to a size of ∼300 base-pairs using a Covaris Focused ultrasonicator (Covaris), followed by end repair using the NEBNext End Repair module (NEB) ...
-
bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was sonicated to a mean fragment size of 350 bp using a Covaris E220 instrument (Covaris, Woburn, MA) and libraries were generated using the xGen Prism library prep kit for NGS (Integrated DNA Technologies ...
-
bioRxiv - Cell Biology 2023Quote: ... 1.0 μg of genomic DNA from proband’s blood is fragmented to an average length of 140 bp using focused acoustic energy (Covaris E210). DNA fragments were subjected to exome capturing using the IDF xGen capture probe panel with an additional “spike-in” of ∼2,500 region which are total 620 kb of RefGene coding regions ...
-
Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The DNA samples were sonicated to a set size of 250 bp with an M220 Focused-ultra sonicator (Covaris, Woburn). Sequencing libraries were generated with NEBNext® UltraTM II DNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Plant Biology 2023Quote: ... Young leaves from a single 3-week-old plant were collected for high-molecular-weight DNA extraction followed by shearing to fragments with an average size of 20 kb using g-TUBE (Covaris). The sheared DNA was then used to construct a PacBio SMRT library following the standard SMRT bell construction protocol ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... PacBio-HiFi libraries were prepared according to the manual “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” with an initial DNA fragmentation by g-Tubes (Covaris) and final library size selection on BluePippin (Sage Science) ...
-
bioRxiv - Genomics 2023Quote: ... The DNA was sheared to approximately 25 kb using a Covaris® G-tube (#520079, Covaris, LLC, Woburn, MA, USA). The sequencing library was prepared using the ligation sequencing DNA v14 method (SQK-LSK114 ...
-
bioRxiv - Cancer Biology 2021Quote: ... One µg of genomic DNA was sheared to a mean fragment length of about 220 bp using focused acoustic energy (Covaris E220). The size distribution of the fragmented sample was determined by using the Caliper LabChip GX system ...
-
bioRxiv - Microbiology 2019Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro-TUBE according to manufacturer’s recommended settings (Covaris, Woburn, MA). Sheared DNA was purified with 1x SPRIselect beads according to manufacturer’s instructions (Beckman Coulter ...