Labshake search
Citations for Covaris :
1 - 50 of 57 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... (4) DNA was sheared to a size of 300-500bp using Covaris LE220 machine (Covaris) at the conditions ...
-
bioRxiv - Cell Biology 2019Quote: ... DNA (3 μg) was sonicated at intensity 4 for 200 cycles per burst for 55 s (Covaris S2), and DNA fragments were end-repaired ...
-
bioRxiv - Genomics 2022Quote: ... Chromatin was recovered after a centrifuge step (13,400 rcf, 20 min, 4°C) and was subjected to sonication on a S220 focused-ultrasonicator (Covaris) (180s ON – 30s OFF – 180s ON ...
-
bioRxiv - Immunology 2021Quote: Ten million naïve or LPS-stimulated (0.1mg/mL for 4 hours) human peripheral blood-derived macrophages were cross-linked and processed using truChIP kit (Covaris Inc), as described in detail before(Mehta et al. ...
-
bioRxiv - Molecular Biology 2022Quote: ... of a 100mg/mL stock in DMSO was sonicated into 30uL PBS at 4°C using an S220 Focused-Ultrasonicator (Covaris) with sonication at peak power = 140 ...
-
bioRxiv - Genomics 2022Quote: ... the resulting 3C templates were purified using AMPure XP beads (Agencourt) and 4 µg of each template was sheared on a Covaris M220 focused-ultrasonicator (Covaris) to achieve 300 bp DNA fragments ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10mM hydroxyflutamide for 3 or 16 hours and nuclear fractions were sonicated using a waterbath sonicator at 4°C (Covaris). Supernatants were precleared using Protein G DynaBeads (Thermo ...
-
bioRxiv - Cancer Biology 2021Quote: ... 200 cycles per burst for 30 min at 4° C to obtain 100-500 base pair chromatin fragments (Covaris, Woburn, CA). Nuclear sheared lysates were clarified by centrifugation at 15,000 x g for 10 min at 4° C ...
-
bioRxiv - Physiology 2021Quote: ... The resuspended chromatin was sonicated into fragments (200 to 500 bps long) at 4 C using an ultrasonic crusher (Covaris, Massachusetts, USA). The sheared chromatin was immunoprecipitated with anti-HA antibodies (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: Myocardium proteins were extracted from cryo-fractured tissue using an automated tissue pulverizer (cyroPREP Tissue Disruption System, Covaris model CP02) prior to the addition of urea lysis buffer and sonication.
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... 5 µg of sperm DNA from 4 CT and 4 Cort F0 mice were fragmented by centrifugation for 60 s at 7200 rpm using g-TUBES (Covaris, Woburn, MA, USA) to obtain 8 kb-long fragments ...
-
bioRxiv - Genomics 2024Quote: Approximately 4.4 μg of HMW DNA was processed with DNA shearing through 6 passes of centrifugation in a g-tube (Covaris Part No. 520079) at 2,000 x g for 2 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell lysate was sonicated by Covaris S220 (Covaris ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were placed into microTUBE-15 (Covaris) microtubes with TPP buffer (Covaris ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were placed into microTUBE-15 (Covaris) microtubes with TPP buffer (Covaris ...
-
bioRxiv - Neuroscience 2019Quote: ... Lysate from cells or tissue was fixed in 1% formaldehyde and cross-linked cell lysates were sheared by Covaris in 1% SDS lysis buffer to generate chromatin fragments with an average length of 300bp ...
-
bioRxiv - Cell Biology 2022Quote: ... The cell mixture was lysed and sonicated by Covaris to obtain an average chromatin size of 200-700 bp ...
-
bioRxiv - Microbiology 2023Quote: ... Cell lysis was performed with a Tissue Homogenizer (Covaris). Subsequent tryptic digestion ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were sonicated using S220 Focused-ultrasonicator (Covaris, MA, USA) and cell lysates were centrifuged at 14,000 rpm for 15 min at 4 °C ...
-
bioRxiv - Genomics 2021Quote: ... Fixed cells were lysed and chromatin was sonicated by Covaris with the following parameters ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were lysed and sonicated with the S2 sonicator (Covaris) to shear the chromatin into 100-300 bp fragments following the program stated in the protocol ...
-
bioRxiv - Immunology 2023Quote: ... Cells were lysed and chromatin was sonicated (Covaris E220 sonicator) to a size range of 200–500 bp ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell lysates were sonicated in a 96 microTUBE Rack (Covaris, #500282) using a Covaris E220 for 22 cycles with the following settings ...
-
bioRxiv - Immunology 2024Quote: ... Using the truChIP High Cell Chromatin Shearing kit (Covaris, United States), cells were prepared for sonication according to the Covaris protocol ...
-
bioRxiv - Microbiology 2020Quote: ... high molecular weight tick cell DNA was fragmented using G-Tubes (Covaris), followed by library preparation (LibMinION_1 ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were fixed and harvested using the truChIP chromatin shearing kit (Covaris) and IP was conducted according to Richard Myers lab ChIP-seq protocol ...
-
bioRxiv - Systems Biology 2023Quote: ... The IFNg-treated cells were sonicated in AFA-tube TPX strips (Covaris), using a Covaris LE220R-Plus for 300 s at 325 peak power with a duty factor of 50 % ...
-
bioRxiv - Genetics 2023Quote: The resuspended cells were transferred to 1 ml milliTUBE AFA Fiber (Covaris) and sonicated on M220 Focused-ultrasonicator (Covaris ...
-
bioRxiv - Genomics 2020Quote: ... The pelleted cells were resuspended in 130 μl of the sonication buffer (Covaris, 10 mM Tris-HCl ...
-
bioRxiv - Genomics 2021Quote: ... cells were resuspended in nuclei lysis buffer and sonicated using an AFA sonicator (Covaris). Immunoprecipitation was performed using a H3K27ac antibody (Abcam ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell suspensions were sonicated for 3 minutes in 1 ml Covaris tubes (Covaris, 520130) using Covaris S220 with the following settings ...
-
bioRxiv - Genomics 2021Quote: ... Cells were fixed in 1% formaldehyde and sonicated using the truChIP Chromatin Shearing Kit (Covaris) on the ME220 Focused-Ultrasonicator (Covaris ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were sonicated and lysed for 5 minutes using the LE220-Plus focused-ultrasonicator (COVARIS) using 350 power peak intensity (PIP) ...
-
bioRxiv - Genetics 2020Quote: The isolated genomic DNAs (~200 ngs/25K cell) were sonicated into 150 bp fragments (Covaris) and the entire reaction product was mixed with 20 μL of Streptavidin magnetic beads (Invitrogen 65001 ...
-
ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Cells were then lysed using lysis buffer solutions and sonicated with the M220 ultrasonicator (Covaris) in 1 mL tube with an AFA Fiber and the following conditions (cycles/burst = 200 ...
-
bioRxiv - Neuroscience 2021Quote: ... The cells were then prepared for sonication using the truChIP chromatin shearing kit (Covaris Cat# 520154). Briefly ...
-
bioRxiv - Genetics 2022Quote: ... Cells were de-frozen on ice and chromatin was prepared following the TruChIP protocol from Covaris, as described in (Cohen et al ...
-
bioRxiv - Genetics 2022Quote: ... Nuclei were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). Sonicated chromatin was transferred to 1.5mL microcentrifuge tubes and Triton-X 100 was added (1% final v/v ...
-
bioRxiv - Neuroscience 2021Quote: ... ILPFC samples were fixed in 1% formaldehyde and cross-linked cell lysates were sheared by Covaris in 1% SDS lysis buffer to generate chromatin fragments with an average length of 300bp by using Peak Power ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). Lysates were transferred to tubes and Triton X-100 was added (final 1% ...
-
bioRxiv - Genomics 2020Quote: ... Lysed cells were then diluted in 900mL TE buffer and sonicated using focused beam ultrasound sonicator (COVARIS). Sonicated samples were repeated for extended periods of time (upto 1.5 hours ...
-
bioRxiv - Genetics 2022Quote: ... The cells were incubated on ice for 10 minutes and sonicated with an S220 Focused-Ultrasonicator (Covaris), using the software SonoLab 7.2 with the following program 10 repeats of treatment with a Peak Power= 200 ...
-
bioRxiv - Cell Biology 2020Quote: ... Sonication of cell lysis was done by a ME220 Focused-ultrasonicator using truChIP Chromatin Shearing Tissue Kit (Covaris). Chromatin immunoprecipitation (ChIP ...
-
bioRxiv - Genomics 2019Quote: ... Genomic DNA from FACS-sorted cells was randomly sheared to 100-500 bp in a microtube (Covaris; 520045) using a Covaris S220 device (400 sec ...
-
bioRxiv - Genomics 2021Quote: One microgram of genomic DNA from the lymphoblastoid Coriell-NA12878 cell line was sheared using g-Tube (Covaris) to obtain high molecular weight fragments ranging from 50kb to 160 kb ...
-
bioRxiv - Genetics 2022Quote: ... Cell nuclei were resuspended in 2 mL 0.4% SDS shearing buffer for sonication with a Covaris S220X sonicator (Covaris) for 7 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were washed two times with PBS and then sonicated for 40 min (S220 focused- ultrasonicator, Covaris, Woburn, MA). After removal of cell debris ...
-
bioRxiv - Immunology 2020Quote: ... cells were washed twice in ice-cold PBS and lysed using the enclosed lysis buffer and sonicated by Covaris S2 (intensity ...
-
bioRxiv - Cell Biology 2020Quote: ... Chromatin of cell lysis was sheared using truChIP™ Chromatin Shearing Kit according its manual by ME220 Focused-ultrasonicator (Covaris). The sheared chromatin was then incubated with anti-ARID1A antibody and purified by using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore ...
-
bioRxiv - Immunology 2021Quote: ... B cell cultures were harvested at d2 and chromatin extracts were prepared using the truChIP Chromatin Shearing Reagent Kit (Covaris). 100μg of sonicated DNA protein complexes were used for immunoprecipitations with anti-IRF5 (Abcam #ab21689) ...