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Citations for Covaris :
51 - 78 of 78 citations for 8 4 Chlorophenylthio guanosine 3' 5' cyclic Monophosphate triethylammonium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... and chromatin sonicated using the Diagenode Bioruptor (set to high intensity, 3 times 7 cyles (30sec ON / 30 sec OFF) or the S220 Focused-ultrasonicator (Covaris) for 20 min at peak power 110 W ...
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bioRxiv - Genomics 2020Quote: ... 5 ug genomic DNA was sheared to ∼15 kb fragments by a g-TUBE (#520079, Covaris) centrifugation (2,000 Xg ...
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bioRxiv - Developmental Biology 2019Quote: ... in 1× fixing buffer for 5 min at room temperature according to the vendor’s manual (Covaris). Crosslinked cells were then resuspended in 1× shearing buffer and sonicated with Covaris E220 for 780 sec using the following settings ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µg of genomic DNA was fragmented using a Covaris E220 (Covaris, Inc. Woburn, MA, USA) with the following settings ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS ...
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bioRxiv - Microbiology 2022Quote: ... DNA was sheared (plasmid: ∼0.5–2kb; gDNA: 5–10 kb) using g-TUBEs (Covaris, Woburn, Massachusetts, USA) and sheared DNA was used to prepare SMRTbell template sequencing libraries ...
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bioRxiv - Systems Biology 2023Quote: DNA (5 µg) was fragmented by sonication to 200 bp with the M220 Focused-Ultrasonicator (Covaris, USA). End-repair ...
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bioRxiv - Genomics 2022Quote: ... Chromatin was recovered after a centrifuge step (13,400 rcf, 20 min, 4°C) and was subjected to sonication on a S220 focused-ultrasonicator (Covaris) (180s ON – 30s OFF – 180s ON ...
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bioRxiv - Genetics 2019Quote: ... Cells were resuspended in 1 ml shearing buffer (10 mM Tris pH 8.0, 5 mM EDTA, 0.1% SDS) and sonicated (Covaris) with the following settings (Peak Incident Power = 100 W ...
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bioRxiv - Immunology 2021Quote: Ten million naïve or LPS-stimulated (0.1mg/mL for 4 hours) human peripheral blood-derived macrophages were cross-linked and processed using truChIP kit (Covaris Inc), as described in detail before(Mehta et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... of a 100mg/mL stock in DMSO was sonicated into 30uL PBS at 4°C using an S220 Focused-Ultrasonicator (Covaris) with sonication at peak power = 140 ...
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bioRxiv - Genomics 2022Quote: ... the resulting 3C templates were purified using AMPure XP beads (Agencourt) and 4 µg of each template was sheared on a Covaris M220 focused-ultrasonicator (Covaris) to achieve 300 bp DNA fragments ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 cycles per burst for 30 min at 4° C to obtain 100-500 base pair chromatin fragments (Covaris, Woburn, CA). Nuclear sheared lysates were clarified by centrifugation at 15,000 x g for 10 min at 4° C ...
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bioRxiv - Physiology 2021Quote: ... The resuspended chromatin was sonicated into fragments (200 to 500 bps long) at 4 C using an ultrasonic crusher (Covaris, Massachusetts, USA). The sheared chromatin was immunoprecipitated with anti-HA antibodies (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: 800 ng of genomic DNA isolated from week 2 and week 5 samples was sonicated using an E220 focused ultrasonicator (Covaris) to produce 400 bp fragments which were purified using Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Neuroscience 2023Quote: ... (100 µL per well) and sonicated for 5 min using the Covaris FPPE protocol using the R230 Focused Ultrasonication Device from Covaris (peak power ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 5 µg of sperm DNA from 4 CT and 4 Cort F0 mice were fragmented by centrifugation for 60 s at 7200 rpm using g-TUBES (Covaris, Woburn, MA, USA) to obtain 8 kb-long fragments ...
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bioRxiv - Genomics 2024Quote: Approximately 4.4 μg of HMW DNA was processed with DNA shearing through 6 passes of centrifugation in a g-tube (Covaris Part No. 520079) at 2,000 x g for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... Extracted chromatin was sheared in SDS lysis buffer (200 μl 1× 109 trophozoites and 5×108 gametocytes) to obtain a fragment size of 100-150bp using an M220 focused-ultrasonicator (Covaris Inc.) using the following settings ...
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bioRxiv - Microbiology 2021Quote: ... DNA was sheared to an average length of approximately 5-10 kb (genomic DNA) using g-TUBEs (Covaris, Woburn, MA, USA) and SMRTbell template sequencing libraries were prepared using sheared DNA ...
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bioRxiv - Microbiology 2023Quote: ... were subjected to soni cation using the Covaris S220 (Power = 140, Duty = 5, Cycles/Burst = 200) for 18 minutes with microTUBE 500 AFA tubes (Covaris 520185) and each sample was incubated with 5 µg anti-FLAG M2 antibody (Millipore A2220 ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (5-10 µg) samples were sheared to an average size of 20 kbp via G-tube (Covaris; Woburn, MA, USA), end repaired and ligated to hairpin barcoded adapters prior to sequencing ...
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bioRxiv - Genomics 2024Quote: ... a dilution of 5 µg HMW DNA in 120 µL elution buffer was transferred to a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation with 1,990 x g for 2 min ...
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bioRxiv - Genetics 2024Quote: ... of purified DNA were fragmented to ∼350bp using the Covaris S2 system (10% duty cycle, intensity 5 for 2x 45 sec in Covaris AFA tubes) followed by a concentration step with Zymo columns (DNA Clean & Concentrator) ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The gRNA was fragmented into approximately 200 nt long fragments using an S220 Focused-ultrasonicator (Duty Cycle 10%; Intensity 5; Peak Incident Power 175 Watts; Cycles per Burst 200; Processing time 200 s; Covaris, Woburn, MA, USA). The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: DNA shearing was first performed from 5 µg HMW DNA in 120 µL elution buffer using a g-tube (Covaris Part No. 520079) with 6 passes of centrifugation at 1,990 x g for 2 min ...