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Citations for Covaris :
1 - 50 of 138 citations for 7 Chloro 2 4 dimethyl 1 8 naphthyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... DNA was fragmented into 2–8 kb pieces using focused ultrasonication (Covaris E220 with miniTUBE red). DNA Fragments were subsequently cloned into a PCR-linearized pZE21 expression vector (primers 1 and 2 ...
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bioRxiv - Genetics 2022Quote: ... 8 kb was done using g-TUBE (Covaris) following manufacturer protocols ...
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bioRxiv - Genomics 2020Quote: ... 8 μg of gDNA was sheared using g-Tubes (Covaris) and concentrated with the AMPure PB magnetic beads.
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bioRxiv - Evolutionary Biology 2023Quote: ... 8°C) and Covaris microTUBE Snap-Cap tubes (Covaris 520045). Sequencing libraries were produced using the KAPA HyperPrep Kit (Roche 07137923001 ...
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bioRxiv - Neuroscience 2020Quote: ... Aggregates were then diluted to a concentration of 100 µg/mL with 1x HBSS buffer followed by three 10s pulses of sonication using a 1/8” probe equipped QS5 Sonicator (Covaris, Woburn, MA). For cell culture experiments ...
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bioRxiv - Genetics 2020Quote: ... using a Covaris S220 instrument with SonoLab software version 7 (Covaris, Inc., USA), to recover fragmented DNA between 150-200 bp ...
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bioRxiv - Microbiology 2020Quote: ... then fragmented at 8 kbp with g-TUBEs (Covaris, Woburn, MA) to homogenized DNA fragments lengths increasing accuracy of input DNA molarity calculation to maximize yields ...
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bioRxiv - Genomics 2020Quote: ... 8 kb and 20 kb fragmentation was done using g-TUBE (Covaris) following manufacturer protocols ...
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bioRxiv - Genomics 2022Quote: ... 1-2 micrograms of high molecular weight DNA was sheared using g-tube (Covaris, Woburn, MA) to 5-9 Kbp at a 7000 RPM and size selected using the 0.75% DF 3-10kb Marker S1-Improved Recovery cassette definition on the Blue Pippin (Sage Science) ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA was sheared to ~8 KB using g-tubes (Covaris, Woburn, MA, USA) and size-selected with the Blue Pippin system (Sage Science ...
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bioRxiv - Microbiology 2023Quote: ... 8 µg genomic DNA was sheared using g-tubes from Covaris (Woburn, MA, USA) according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The epidermis was homogenised in 8 M urea buffer with an ultrasonicator (S220X, Covaris, Brighton, UK) with 175 W peak power for 8 min ...
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bioRxiv - Plant Biology 2023Quote: ... and chromatin sonicated using the Diagenode Bioruptor (set to high intensity, 3 times 7 cyles (30sec ON / 30 sec OFF) or the S220 Focused-ultrasonicator (Covaris) for 20 min at peak power 110 W ...
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bioRxiv - Genomics 2020Quote: ... A total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
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bioRxiv - Genomics 2023Quote: ... Sonicate the chromatin until sufficiently sheared (130µL, 5% duty cycle, 75W peak incident power, 200 cycles per burst, 7°C, for 5 minutes using Covaris Focus-Ultrasonicator M220). The immunoprecipitation step included ...
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bioRxiv - Genomics 2022Quote: ... For the paired end library a total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
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bioRxiv - Immunology 2021Quote: ... in immunoprecipitation buffer (0.1% Triton X-100, 0.1 M Tris-HCl pH 8, 0.5 mM EDTA, and 0.15 M NaCl in 1Å~ Covaris D3 buffer) at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Samples were sheared on the Covaris® LE220plus using the 8 microTUBE strip V1 (PM# 520053; Covaris, Woburn, MA) with the base pair (bp ...
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bioRxiv - Bioengineering 2021Quote: ... All samples were stored in low TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8) and were fragmented by Covaris ultrasonicator to have a mean size of 150 bp except cfDNA ...
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bioRxiv - Genomics 2019Quote: ... for preparation of 15 kbp libraries 8 µg genomic DNA and 1.4 µg plasmid DNA was sheared using g-tubes™ from Covaris, Woburn ...
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bioRxiv - Plant Biology 2019Quote: ... 6 μg genomic DNA in a total of 150 μl nuclease free water was sheared into ~8 kb with g-TUBEs (520079, Covaris) by following manufacturer’s instruction.
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bioRxiv - Genomics 2019Quote: ... DNA was then resuspended in 10 mM T ris-HCl pH 8 and 5 ug aliquots were sonicated by Covaris S2 to fragment sizes in the range of 450-550 bp ...
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bioRxiv - Microbiology 2022Quote: ... post-RCA DNA was debranched and fragmented to 6-8 kb-sized fragments using the above-mentioned protocol with the modification of the g-TUBE (Covaris) being centrifuged at 13200 rpm (16363 RCF ...
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bioRxiv - Genomics 2024Quote: 120 μl of DNA sample with around 8-10 μg DNA was transferred to a g-tube (Covaris Part No. 520079). The sample was then proceeded to 6 passes of centrifugation with 2,000 xg of 2 min ...
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bioRxiv - Genomics 2020Quote: ... two micrograms of high molecular weight genomic DNA from each sample was sheared using g0-tube to ∼8 Kb (Covaris, Woburn, MA). These sheared gDNA samples were size selected to include 5 Kb-9 Kb fragments with Blue Pippin (Sage Science ...
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bioRxiv - Genomics 2019Quote: ... and genomic DNA from GM12878 lymphoblast cell line (Coriell Institute) were first sheared to an average fragment size of 8 kb using g-tubes (Covaris Cat. 520079). The fragmented DNA was PCR amplified to generate unmethylated DNA using the first steps of low input ligation kit SQK-LWP001 (ONT) ...
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bioRxiv - Genetics 2022Quote: ... Three microgram of HMW gDNA was sheared to an average size distribution of ~8 kb using the Covaris g-TUBE (Covaris, MA, U.S.A.) in an Eppendorf 5810 R centrifuge at 5,000 rpm for a total of 2 minutes ...
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bioRxiv - Genetics 2021Quote: ... and 2 μg DNA was Fragmented by g-TUBE (Covaris). DNA repair was performed using NEBNext FFPE DNA Repair Mix (M6630L ...
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bioRxiv - Genomics 2020Quote: ... and then split over 2 Covaris microTubes (Covaris, Part #520045). Cells were then sonicated ...
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bioRxiv - Genetics 2022Quote: ... CA, USA. Three microgram of HMW gDNA was sheared to an average size distribution of ~8 kb using the Covaris g-TUBE (Covaris, MA, U.S.A.) in an Eppendorf 5810 R centrifuge at 5,000 rpm for a total of 2 minutes ...
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bioRxiv - Genetics 2021Quote: ... DNA (2 μg) was sheared in a S220 Focused-ultrasonicator (Covaris), DNA fragments were purified using the DNA Clean &Concentrator™-5 kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2023Quote: ... (4) DNA was sheared to a size of 300-500bp using Covaris LE220 machine (Covaris) at the conditions ...
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bioRxiv - Genomics 2022Quote: ... High molecular weight DNA (0.5-2 ug) was sheared using g-tubes (Covaris) and size selected using the 0.75% DF 3-10 Kbp Marker S1-Improved Recovery cassette definition on the Blue Pippin (Sage Science) ...
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bioRxiv - Cell Biology 2019Quote: ... DNA (3 μg) was sonicated at intensity 4 for 200 cycles per burst for 55 s (Covaris S2), and DNA fragments were end-repaired ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA was sheared using a Covaris S-2 system (Covaris, Inc. Woburn, MA). Sheared DNA fragments were purified with Agencourt AMPure XP beads ...
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bioRxiv - Cell Biology 2023Quote: ... 2 × 106 BMDMs were sonicated using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) at a high power setting (peak power ...
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bioRxiv - Genomics 2022Quote: ... Chromatin was recovered after a centrifuge step (13,400 rcf, 20 min, 4°C) and was subjected to sonication on a S220 focused-ultrasonicator (Covaris) (180s ON – 30s OFF – 180s ON ...
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bioRxiv - Microbiology 2019Quote: ... 2-5 µg of genomic DNA was sheared with a Covaris M220 Focused-ultrasonicator (Covaris) into 400-600bp fragments ...
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bioRxiv - Developmental Biology 2022Quote: ... a minimum of 630 ng up to 2 ug of library were sonicated by Covaris E220 ultrasonicator at the following conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA (2 µg) was fragmented to an average of 500 bp by sonication (Covaris) and ligation of full-length barcoded Illumina adapters was performed using the TruSeq DNA PCR-free kit (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue samples were powderized with 2 pulses (at maximum intensity) using a CryoPrepTM system (Covaris). Equal amounts of powder were transferred to a pre-cooled 2 mL tube ...
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bioRxiv - Immunology 2021Quote: Ten million naïve or LPS-stimulated (0.1mg/mL for 4 hours) human peripheral blood-derived macrophages were cross-linked and processed using truChIP kit (Covaris Inc), as described in detail before(Mehta et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... of a 100mg/mL stock in DMSO was sonicated into 30uL PBS at 4°C using an S220 Focused-Ultrasonicator (Covaris) with sonication at peak power = 140 ...
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bioRxiv - Genomics 2022Quote: ... the resulting 3C templates were purified using AMPure XP beads (Agencourt) and 4 µg of each template was sheared on a Covaris M220 focused-ultrasonicator (Covaris) to achieve 300 bp DNA fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... 10mM hydroxyflutamide for 3 or 16 hours and nuclear fractions were sonicated using a waterbath sonicator at 4°C (Covaris). Supernatants were precleared using Protein G DynaBeads (Thermo ...
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 2 ml shearing buffer and sonicated using a Focused Ultrasonicator M220 (Covaris). Sonication was performed in two rounds using two different sonication conditions ...
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bioRxiv - Microbiology 2022Quote: ... 2 µg of genomic DNA was fragmented using an ultra-focused sonicator (Covaris M220, Covaris, Inc.) in microTUBE-50 AFA Fiber Screw-Cap tubes ...
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bioRxiv - Microbiology 2022Quote: ... 2 µg of genomic DNA was fragmented using an ultra-focused sonicator (Covaris M220, Covaris, Inc.) in microTUBE-50 AFA Fiber Screw-Cap tubes ...
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bioRxiv - Cell Biology 2020Quote: ... 1:50 cOmplete) by a Focused-ultrasonicator (Covaris M220, 1 ml) to yield chromatin fragments with an average size of 600 bp.
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bioRxiv - Cancer Biology 2021Quote: ... 200 cycles per burst for 30 min at 4° C to obtain 100-500 base pair chromatin fragments (Covaris, Woburn, CA). Nuclear sheared lysates were clarified by centrifugation at 15,000 x g for 10 min at 4° C ...