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Citations for Covaris :
1 - 50 of 119 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... the pellet was resuspended to 350 μl of 1 M buffer B and sonicated (Covaris S220 sonicator ...
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bioRxiv - Developmental Biology 2019Quote: ... in 1× fixing buffer for 5 min at room temperature according to the vendor’s manual (Covaris). Crosslinked cells were then resuspended in 1× shearing buffer and sonicated with Covaris E220 for 780 sec using the following settings ...
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bioRxiv - Cell Biology 2023Quote: ... The microTUBE AFA Fiber Crimp-Cap 6×16mm (Covaris, SKU: 520052) was used with the sonicator ...
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bioRxiv - Genetics 2019Quote: ... Cells were resuspended in 1 ml shearing buffer (10 mM Tris pH 8.0, 5 mM EDTA, 0.1% SDS) and sonicated (Covaris) with the following settings (Peak Incident Power = 100 W ...
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bioRxiv - Immunology 2021Quote: ... B cell cultures were harvested at d2 and chromatin extracts were prepared using the truChIP Chromatin Shearing Reagent Kit (Covaris). 100μg of sonicated DNA protein complexes were used for immunoprecipitations with anti-IRF5 (Abcam #ab21689) ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2023Quote: ... Sonicate the chromatin until sufficiently sheared (130µL, 5% duty cycle, 75W peak incident power, 200 cycles per burst, 7°C, for 5 minutes using Covaris Focus-Ultrasonicator M220). The immunoprecipitation step included ...
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bioRxiv - Genomics 2023Quote: ... genomic DNA was disrupted randomly with 6 kb-20 kb fragments by g-TUBE (Covaris, Woburn, MA, USA) and sequenced by the PacBio Sequel platform ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina, PT0017_Covaris_20ng_Illumina ...
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bioRxiv - Genomics 2020Quote: ... Library preparation followed ultrasonic fragmentation (Covaris: 50 s, 5% duty factor) using the Illumina TruSeq DNA PCR-Free Sample Preparation Kit with an insert size of 350 bp ...
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bioRxiv - Genomics 2021Quote: ... 120 μL hydrated DNA was transferred to a Covaris microtube (AFA fiber pre-slit snap cap, 6×16 mm) and sheared using a probe sonicator (Covaris S220 focused ultrasonicator instrument ...
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bioRxiv - Microbiology 2022Quote: ... post-RCA DNA was debranched and fragmented to 6-8 kb-sized fragments using the above-mentioned protocol with the modification of the g-TUBE (Covaris) being centrifuged at 13200 rpm (16363 RCF ...
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bioRxiv - Cancer Biology 2023Quote: ... material was adjusted to a final volume 100μL and sonicated in microTUBE AFA Fiber screw cap tubes (6 mm × 16 mm, Covaris, 520096) using the Covaris S220 Focused-ultrasonicator at a maximum power for 15 seconds ...
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bioRxiv - Genomics 2023Quote: ... Samples in each batch were pulverized in four groups of 10 or 11 samples (each sample weighing between 6-9 mg) using a CP02 cryoPREP automated dry pulverizer (Covaris 500001) and resuspended in 1 mL of ice-cold PBS ...
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bioRxiv - Genetics 2021Quote: ... Both cell lysate and debris were collected and subsequently transferred to an AFA fiber pre-slit snap-cap [6 x 15mm] microtube (Covaris, Woburn, MA) for additional cellular lysis and DNA shearing.
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bioRxiv - Neuroscience 2019Quote: ... 5.0, bursts per second: 200, duration: 120 s, mode: frequency sweeping, power: 23 W, temperature: 5.5–6°C; Covaris Inc., Woburn, MA). Subsequently ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5-25 µg of DNA was sheared using a Covaris g-TUBE (Covaris) and gel size-selected for 1.5 kb ...
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bioRxiv - Genomics 2024Quote: Approximately 4.4 μg of HMW DNA was processed with DNA shearing through 6 passes of centrifugation in a g-tube (Covaris Part No. 520079) at 2,000 x g for 2 min ...
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bioRxiv - Genomics 2019Quote: ... Approximately 5 µg of genomic DNA was fragmented with a focused ultrasonicator system (Covaris). Fragmented DNA was used to prepare Illumina paired-end (PE ...
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bioRxiv - Developmental Biology 2020Quote: ... All samples yielding at least 5 ng of cDNA were sheared by sonication (Covaris, S220/E220 focused-ultrasonicator ...
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bioRxiv - Genomics 2022Quote: ... 5-10 mg of snap-frozen tissue was loaded in tissueTUBE-TT05 (Covaris 520071) and cryopulverized using the Covaris CP02 cryoPREP Automated Dry Pulverizer following manufacturer’s procedure ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were sonicated using a Covaris® S220 focused-ultrasonicator in microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16 mm tubes (Covaris®, Woburn, MA, USA) with the following program parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... input DNA was sheared to an average size of 5-10 kb using gTubes (Covaris) and concentrated using 0.6V Ampure beads (Pacific Biosciences) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were sonicated and lysed for 5 minutes using the LE220-Plus focused-ultrasonicator (COVARIS) using 350 power peak intensity (PIP) ...
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bioRxiv - Microbiology 2019Quote: ... 2-5 µg of genomic DNA was sheared with a Covaris M220 Focused-ultrasonicator (Covaris) into 400-600bp fragments ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg of DNA was sheared to 10 kbp using a g-Tube (Covaris, USA), and a SMRTbell library was made with the SMRTbell template prep kit 1.0 (Pacific Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... approximately 50 µL of gDNA (2.5 – 5 µg) was sheared with a sonicator (Covaris S2) in a 130 µL cuvette with the following parameters ...
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bioRxiv - Genomics 2020Quote: ... 5 ug genomic DNA was sheared to ∼15 kb fragments by a g-TUBE (#520079, Covaris) centrifugation (2,000 Xg ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µg of genomic DNA was fragmented using a Covaris E220 (Covaris, Inc. Woburn, MA, USA) with the following settings ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS ...
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bioRxiv - Microbiology 2022Quote: ... DNA was sheared (plasmid: ∼0.5–2kb; gDNA: 5–10 kb) using g-TUBEs (Covaris, Woburn, Massachusetts, USA) and sheared DNA was used to prepare SMRTbell template sequencing libraries ...
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bioRxiv - Systems Biology 2023Quote: DNA (5 µg) was fragmented by sonication to 200 bp with the M220 Focused-Ultrasonicator (Covaris, USA). End-repair ...
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bioRxiv - Cell Biology 2020Quote: ... 1:50 cOmplete) by a Focused-ultrasonicator (Covaris M220, 1 ml) to yield chromatin fragments with an average size of 600 bp.
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bioRxiv - Microbiology 2020Quote: 800 ng of genomic DNA isolated from week 2 and week 5 samples was sonicated using an E220 focused ultrasonicator (Covaris) to produce 400 bp fragments which were purified using Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2019Quote: ... DNA was then resuspended in 10 mM T ris-HCl pH 8 and 5 ug aliquots were sonicated by Covaris S2 to fragment sizes in the range of 450-550 bp ...
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bioRxiv - Neuroscience 2023Quote: ... (100 µL per well) and sonicated for 5 min using the Covaris FPPE protocol using the R230 Focused Ultrasonication Device from Covaris (peak power ...
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bioRxiv - Microbiology 2020Quote: ... Extracted chromatin was sheared in SDS lysis buffer (200 μl 1× 109 trophozoites and 5×108 gametocytes) to obtain a fragment size of 100-150bp using an M220 focused-ultrasonicator (Covaris Inc.) using the following settings ...
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bioRxiv - Microbiology 2021Quote: ... DNA was sheared to an average length of approximately 5-10 kb (genomic DNA) using g-TUBEs (Covaris, Woburn, MA, USA) and SMRTbell template sequencing libraries were prepared using sheared DNA ...
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bioRxiv - Microbiology 2023Quote: ... were subjected to soni cation using the Covaris S220 (Power = 140, Duty = 5, Cycles/Burst = 200) for 18 minutes with microTUBE 500 AFA tubes (Covaris 520185) and each sample was incubated with 5 µg anti-FLAG M2 antibody (Millipore A2220 ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was resuspended in water and sheared into 3-5 kb fragments using the S220 Focused-ultrasonicator (Covaris Inc., MA, USA).
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (5-10 µg) samples were sheared to an average size of 20 kbp via G-tube (Covaris; Woburn, MA, USA), end repaired and ligated to hairpin barcoded adapters prior to sequencing ...
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bioRxiv - Genomics 2024Quote: ... a dilution of 5 µg HMW DNA in 120 µL elution buffer was transferred to a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation with 1,990 x g for 2 min ...
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bioRxiv - Genetics 2024Quote: ... of purified DNA were fragmented to ∼350bp using the Covaris S2 system (10% duty cycle, intensity 5 for 2x 45 sec in Covaris AFA tubes) followed by a concentration step with Zymo columns (DNA Clean & Concentrator) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were resuspended in 1 mL LB3 and transferred to a 1 ml milliTUBE (Covaris). Chromatin was sheared to a range of 300- 600 base-pair fragments using a Covaris E220 evolution Focused ultrasonicator with the following parameters ...
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bioRxiv - Genomics 2024Quote: ... nuclei were resuspended in 1 mL LB3 and transferred to a 1 mL milliTUBE (Covaris) for shearing ...
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bioRxiv - Molecular Biology 2023Quote: ... The gRNA was fragmented into approximately 200 nt long fragments using an S220 Focused-ultrasonicator (Duty Cycle 10%; Intensity 5; Peak Incident Power 175 Watts; Cycles per Burst 200; Processing time 200 s; Covaris, Woburn, MA, USA). The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: DNA shearing was first performed from 5 µg HMW DNA in 120 µL elution buffer using a g-tube (Covaris Part No. 520079) with 6 passes of centrifugation at 1,990 x g for 2 min ...
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bioRxiv - Genomics 2022Quote: ... using 1 ml AFA tubes (Covaris). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... Exactly 1 ml nuclei suspension was transferred to a 1 ml milliTUBE with AFA fiber (Covaris, #520130) and sonicated using a Covaris S220 device for a time of 15-20 min and the following settings ...