Labshake search
Citations for Covaris :
1 - 50 of 51 citations for 6 Chloro 9 2 C methyl β D riboFuranosyl 9H purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... Samples in each batch were pulverized in four groups of 10 or 11 samples (each sample weighing between 6-9 mg) using a CP02 cryoPREP automated dry pulverizer (Covaris 500001) and resuspended in 1 mL of ice-cold PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... 5.0, bursts per second: 200, duration: 120 s, mode: frequency sweeping, power: 23 W, temperature: 5.5–6°C; Covaris Inc., Woburn, MA). Subsequently ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... little skate genomic DNA (6.5–9 µg in 150 µl volume) was transferred to a Covaris g-Tube (Covaris, #520079) and sheared in a tabletop centrifuge (Eppendorf ...
-
bioRxiv - Cell Biology 2023Quote: ... The microTUBE AFA Fiber Crimp-Cap 6×16mm (Covaris, SKU: 520052) was used with the sonicator ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were suspended in an optimized solvent extraction mixture of 9:1 (methanol: chloroform) as described elsewhere in detail75 and extracted with a focused ultrasound system (Covaris E220, Woburn, USA). The extraction solutions were evaporated to dryness for 4 hours in a vacuum concentrator and afterwards pellets resuspended with 45 µL in a 1 mM TSP containing deuterated phosphate buffer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... 8°C) and Covaris microTUBE Snap-Cap tubes (Covaris 520045). Sequencing libraries were produced using the KAPA HyperPrep Kit (Roche 07137923001 ...
-
bioRxiv - Genomics 2023Quote: ... genomic DNA was disrupted randomly with 6 kb-20 kb fragments by g-TUBE (Covaris, Woburn, MA, USA) and sequenced by the PacBio Sequel platform ...
-
bioRxiv - Genomics 2021Quote: ... 120 μL hydrated DNA was transferred to a Covaris microtube (AFA fiber pre-slit snap cap, 6×16 mm) and sheared using a probe sonicator (Covaris S220 focused ultrasonicator instrument ...
-
bioRxiv - Microbiology 2022Quote: ... post-RCA DNA was debranched and fragmented to 6-8 kb-sized fragments using the above-mentioned protocol with the modification of the g-TUBE (Covaris) being centrifuged at 13200 rpm (16363 RCF ...
-
bioRxiv - Cancer Biology 2023Quote: ... material was adjusted to a final volume 100μL and sonicated in microTUBE AFA Fiber screw cap tubes (6 mm × 16 mm, Covaris, 520096) using the Covaris S220 Focused-ultrasonicator at a maximum power for 15 seconds ...
-
bioRxiv - Genetics 2021Quote: ... and 2 μg DNA was Fragmented by g-TUBE (Covaris). DNA repair was performed using NEBNext FFPE DNA Repair Mix (M6630L ...
-
bioRxiv - Genomics 2020Quote: ... and then split over 2 Covaris microTubes (Covaris, Part #520045). Cells were then sonicated ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The DNA Hi-C libraries were sheared into 300 bp using a Covaris S220 apparatus (Covaris) and the biotin-labeled fragments were selectively captured by Dynabeads Myone Streptavidin C1 ...
-
bioRxiv - Genetics 2021Quote: ... Both cell lysate and debris were collected and subsequently transferred to an AFA fiber pre-slit snap-cap [6 x 15mm] microtube (Covaris, Woburn, MA) for additional cellular lysis and DNA shearing.
-
bioRxiv - Genetics 2021Quote: ... DNA (2 μg) was sheared in a S220 Focused-ultrasonicator (Covaris), DNA fragments were purified using the DNA Clean &Concentrator™-5 kit (Zymo Research) ...
-
bioRxiv - Genomics 2024Quote: Approximately 4.4 μg of HMW DNA was processed with DNA shearing through 6 passes of centrifugation in a g-tube (Covaris Part No. 520079) at 2,000 x g for 2 min ...
-
bioRxiv - Plant Biology 2021Quote: ... The samples were sonicated using a Covaris® S220 focused-ultrasonicator in microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16 mm tubes (Covaris®, Woburn, MA, USA) with the following program parameters ...
-
bioRxiv - Genomics 2022Quote: ... High molecular weight DNA (0.5-2 ug) was sheared using g-tubes (Covaris) and size selected using the 0.75% DF 3-10 Kbp Marker S1-Improved Recovery cassette definition on the Blue Pippin (Sage Science) ...
-
bioRxiv - Genomics 2022Quote: ... Chromatin was recovered after a centrifuge step (13,400 rcf, 20 min, 4°C) and was subjected to sonication on a S220 focused-ultrasonicator (Covaris) (180s ON – 30s OFF – 180s ON ...
-
bioRxiv - Genomics 2023Quote: ... Hi-C template DNA was sheared to a size of 300-500 bp using microTUBE snap-caps (Covaris, 520045) in a Covaris M220 sonicator and MyOne Streptavidin T1 beads (Life Technologies ...
-
bioRxiv - Genomics 2023Quote: ... Purified DNA was sheared using a Covaris S-2 system (Covaris, Inc. Woburn, MA). Sheared DNA fragments were purified with Agencourt AMPure XP beads ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 × 106 BMDMs were sonicated using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) at a high power setting (peak power ...
-
bioRxiv - Genomics 2021Quote: ... and 0.5 ng of all-C hydroxymethylated pUC19 plasmid DNA (Wisegene) and sonicated to ∼300 bp fragments using a M220 focused ultrasonicator (Covaris), with the following parameters ...
-
bioRxiv - Molecular Biology 2022Quote: ... of a 100mg/mL stock in DMSO was sonicated into 30uL PBS at 4°C using an S220 Focused-Ultrasonicator (Covaris) with sonication at peak power = 140 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10mM hydroxyflutamide for 3 or 16 hours and nuclear fractions were sonicated using a waterbath sonicator at 4°C (Covaris). Supernatants were precleared using Protein G DynaBeads (Thermo ...
-
bioRxiv - Cancer Biology 2021Quote: ... 200 cycles per burst for 30 min at 4° C to obtain 100-500 base pair chromatin fragments (Covaris, Woburn, CA). Nuclear sheared lysates were clarified by centrifugation at 15,000 x g for 10 min at 4° C ...
-
bioRxiv - Microbiology 2019Quote: ... 2-5 µg of genomic DNA was sheared with a Covaris M220 Focused-ultrasonicator (Covaris) into 400-600bp fragments ...
-
bioRxiv - Developmental Biology 2022Quote: ... a minimum of 630 ng up to 2 ug of library were sonicated by Covaris E220 ultrasonicator at the following conditions ...
-
bioRxiv - Molecular Biology 2022Quote: ... genomic DNA (2 µg) was fragmented to an average of 500 bp by sonication (Covaris) and ligation of full-length barcoded Illumina adapters was performed using the TruSeq DNA PCR-free kit (Illumina ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissue samples were powderized with 2 pulses (at maximum intensity) using a CryoPrepTM system (Covaris). Equal amounts of powder were transferred to a pre-cooled 2 mL tube ...
-
bioRxiv - Physiology 2021Quote: ... The resuspended chromatin was sonicated into fragments (200 to 500 bps long) at 4 C using an ultrasonic crusher (Covaris, Massachusetts, USA). The sheared chromatin was immunoprecipitated with anti-HA antibodies (Sigma-Aldrich ...
-
bioRxiv - Genomics 2023Quote: ... Sonicate the chromatin until sufficiently sheared (130µL, 5% duty cycle, 75W peak incident power, 200 cycles per burst, 7°C, for 5 minutes using Covaris Focus-Ultrasonicator M220). The immunoprecipitation step included ...
-
bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 2 ml shearing buffer and sonicated using a Focused Ultrasonicator M220 (Covaris). Sonication was performed in two rounds using two different sonication conditions ...
-
bioRxiv - Genomics 2022Quote: ... 1-2 micrograms of high molecular weight DNA was sheared using g-tube (Covaris, Woburn, MA) to 5-9 Kbp at a 7000 RPM and size selected using the 0.75% DF 3-10kb Marker S1-Improved Recovery cassette definition on the Blue Pippin (Sage Science) ...
-
bioRxiv - Microbiology 2022Quote: ... 2 µg of genomic DNA was fragmented using an ultra-focused sonicator (Covaris M220, Covaris, Inc.) in microTUBE-50 AFA Fiber Screw-Cap tubes ...
-
bioRxiv - Microbiology 2022Quote: ... 2 µg of genomic DNA was fragmented using an ultra-focused sonicator (Covaris M220, Covaris, Inc.) in microTUBE-50 AFA Fiber Screw-Cap tubes ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was fragmented into 2–8 kb pieces using focused ultrasonication (Covaris E220 with miniTUBE red). DNA Fragments were subsequently cloned into a PCR-linearized pZE21 expression vector (primers 1 and 2 ...
-
bioRxiv - Plant Biology 2022Quote: ... The diluted Hi-C samples were fragmented to a mean size of 300 bp by sonication using a Covaris M220 sonication system (Covaris, Woburn, MA, USA) in a milliTUBE 1 ml AFA Fibre (Covaris) ...
-
bioRxiv - Immunology 2023Quote: ... In brief: Blood plasma was transferred to 2 mL AFA glass tubes (Covaris Inc, Woburn, Massachusetts, USA) and mixed with ultra-pure water ...
-
bioRxiv - Genomics 2021Quote: ... 2 µg of each gDNA were sheared using a Covaris M220 Focused-ultrasonicator (Covaris, Inc. Woburn, MA, USA), end-repaired ...
-
bioRxiv - Genetics 2022Quote: ... genomic DNA (2□μg) was diluted in 200 μL of elution buffer and fragmented using g-TUBE (Covaris) by centrifuging at 2400×g for 2□min ...
-
bioRxiv - Genetics 2022Quote: ... Cell nuclei were resuspended in 2 mL 0.4% SDS shearing buffer for sonication with a Covaris S220X sonicator (Covaris) for 7 min ...
-
bioRxiv - Genetics 2019Quote: ... one frozen 2 mm core was pulverized in the pulverization bag using the Covaris CryoPrep system (Covaris, Woburn, MA) once at intensity 3 and once at intensity 4 ...
-
DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... 50 mg of deep-frozen feces sample were transferred into 2 mL AFA glass tubes (Covaris Inc, Woburn, USA) and mixed with 400 μL of ultrapure methanol and 800 μL of MTBE (solvent grade) ...
-
bioRxiv - Genomics 2020Quote: ... A total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
-
bioRxiv - Microbiology 2020Quote: 800 ng of genomic DNA isolated from week 2 and week 5 samples was sonicated using an E220 focused ultrasonicator (Covaris) to produce 400 bp fragments which were purified using Agencourt AMPure XP beads (Beckman Coulter) ...
-
bioRxiv - Microbiology 2022Quote: ... ∼2 μg of genomic DNA was fragmented to a size of ∼300 base-pairs using a Covaris Focused ultrasonicator (Covaris), followed by end repair using the NEBNext End Repair module (NEB) ...
-
bioRxiv - Neuroscience 2020Quote: ... 50 mg of deep-frozen feces sample were transferred into 2 ml adaptive focused acoustics (AFA) glass tubes (Covaris Inc, Woburn, USA) and mixed with 400 μl of ultrapure methanol and 800 μL of Methyl ter-butyl ether (MTBE ...
-
bioRxiv - Genomics 2022Quote: ... For the paired end library a total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
-
bioRxiv - Genomics 2019Quote: ... 5 µg of the genomic DNA extracted from adipose or lung tissues of randomly selected mice (n = 2) were sheared with an S2 ultrasonicator (Covaris, Woburn, MA, USA) in 10 mM Tris buffer (pH 8.0 ...