Labshake search
Citations for Covaris :
201 - 250 of 305 citations for Mouse Proto oncogene Mas MAS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: A total of 60 ng of genomic DNA from each sample was sheared to an average size of 350 bp using a Covaris M220 Focused-ultrasonicator (Covaris, Woburn, MA) with Micro AFA fiber tubes (Covaris ...
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of genomic DNA was fragmented by acoustic shearing to a target value of 250 bp using a Covaris S220 (Covaris, Woburn, MA), according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2019Quote: ... 5.0, bursts per second: 200, duration: 120 s, mode: frequency sweeping, power: 23 W, temperature: 5.5–6°C; Covaris Inc., Woburn, MA). Subsequently ...
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bioRxiv - Genomics 2020Quote: ... The DNA was sheared to a mean fragment length of 20-30 kb with Covaris g-TUBEs (Covaris Inc., Woburn, MA, USA). The length of the DNA was measured with a TapeStation (4200 ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... around 30 brains in 110µl lysis buffer in one microtube-130 AFA Fiber Pre-Slit Snap-Cap (Catalog #520045, Covaris, Inc. Woburn, MA) were sonicated on a Covaris ME220 sonicator (Catalog # 500295 ...
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bioRxiv - Genomics 2020Quote: ... two micrograms of high molecular weight genomic DNA from each sample was sheared using g0-tube to ∼8 Kb (Covaris, Woburn, MA). These sheared gDNA samples were size selected to include 5 Kb-9 Kb fragments with Blue Pippin (Sage Science ...
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bioRxiv - Cell Biology 2021Quote: ... Each was brought to 5% SDS by volume (~1 ml total final volume) and subjected to complete lysis and DNA shearing using a Covaris Model S220 Focused Acoustic device (Covaris, Woburn MA) in 1ml glass Covaris vials ...
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bioRxiv - Neuroscience 2020Quote: ... Aggregates were then diluted to a concentration of 100 µg/mL with 1x HBSS buffer followed by three 10s pulses of sonication using a 1/8” probe equipped QS5 Sonicator (Covaris, Woburn, MA). For cell culture experiments ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (5-10 µg) samples were sheared to an average size of 20 kbp via G-tube (Covaris; Woburn, MA, USA), end repaired and ligated to hairpin barcoded adapters prior to sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated genomic DNA was mechanically sheared to an average size of 250 bases using a Covaris instrument LE220 (Covaris Inc., Woburn, MA). The fragmented DNA was enzymatically repaired and end-modified with adenosine (NEBNext® Ultra™ II End Repair/dA-Tailing Module ...
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bioRxiv - Neuroscience 2019Quote: ... One microgram of DNA was dissolved in 130 μl of 10 mM Tris–HCl (pH 8.0) and sheared with an S220 focused ultrasonicator (Covaris, Woburn, MA, USA) to yield 500-bp fragments ...
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bioRxiv - Genomics 2019Quote: ... with the following changes: 1 μg of gDNA was sheared to 70-90bp fragments using the Covaris S220 (Covaris Inc., Woburn, MA) and 150bp fragment libraries were constructed according to standard SOLiD Fragment library protocols (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... DNA was sheared to 200 bp to 850 bp (average size = 400 bp) using the Covaris S2 instrument (Covaris, Woburn, MA, USA). DNA was then concentrated to a volume of 50 µL using the DNA Clean & Concentrator kit (Cat ...
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bioRxiv - Genetics 2022Quote: ... Three microgram of HMW gDNA was sheared to an average size distribution of ~8 kb using the Covaris g-TUBE (Covaris, MA, U.S.A.) in an Eppendorf 5810 R centrifuge at 5,000 rpm for a total of 2 minutes ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA from each sample was sheared to an average size of 350 bp using a Covaris M220 Focused-ultrasonicator (Covaris, Woburn, MA) with Micro AFA fiber tubes (Covaris ...
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bioRxiv - Genomics 2023Quote: ... of extracted genomic DNA (gDNA) were fragmented to a median size of ∼300 base pairs using a Covaris ME220 ultrasonic shearing system (Covaris, Woburn, MA). For samples with a DNA integrity number (DIN ...
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bioRxiv - Plant Biology 2023Quote: ... genomic DNA was fragmented to an average size of 500 bp using an M220 Focused-ultrasonicator M220 (Covaris Inc., Woburn, MA. USA). A paired-end library with insert sizes ranging from 450 to 550 bp was constructed using the TruSeq DNA PCR-Free Library Prep kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... tumour tissue and organoid DNA were fragmented to 200 base pair (bp) length using a Covaris E Series instrument (Covaris, MA, USA), while cfDNA was not further fragmented ...
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bioRxiv - Neuroscience 2024Quote: ... was taken to the Duke University Center for Genomic and Computational Biology (GCB) and sheared to achieve 175 bp-long fragments using Covaris S220 (Covaris, Woburn, MA). DNA quality was assessed using Agilent 4200 TapeStation ...
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bioRxiv - Genomics 2022Quote: ... 1 µg of gDNA in 50 µl 1X low-EDTA TE buffer was sheared with a Covaris E220 sonicator (Covaris, Inc., Woburn, MA) to an average of 200 base pairs ...
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bioRxiv - Genomics 2019Quote: ... we prepared the library using 10 μg genomic DNA that was sheared to a size of 20kb fragments by g-tubes (Covaris, Inc., Woburn, MA) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2021Quote: ... SMRTbell libraries were constructed from genomic DNA samples sheared to between 10 and 20 kb using the G-tubes protocol (Covaris; Woburn, MA, USA), end repaired ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Neuroscience 2019Quote: ... and positive INTACT-isolated sample 400 ng of gDNA was brought up to 50 µl volume with 1X low-EDTA TE buffer and sheared with a Covaris E220 sonicator (Covaris, Inc., Woburn, MA) to an average 200 base pair size using the following settings ...
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bioRxiv - Plant Biology 2021Quote: A total amount of 5.2 µg genomic DNA spiked with 26 ng lambda DNA was fragmented by sonication to lengths of 200-400 bp with a Covaris S220 (Covaris, Woburn, MA, USA), followed by end-repair and adenylation ...
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bioRxiv - Pathology 2021Quote: Frozen tissue samples from heart and skeletal muscle were transferred in a prechilled tubes and cryo-pulverized in a Covaris CP02 Automated Dry Pulverizer (Covaris Inc, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA (10 ng to 500 ng) was sheared into fragments of approximately 300-400 bp in a Covaris E210 system (96 well format, Covaris, Inc. Woburn, MA) followed by purification of the fragmented DNA using AMPure XP beads ...
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bioRxiv - Genomics 2019Quote: ... 100-ng of genomic DNA from each sample was sheared to fragment sizes ranging from 300∼500-kb by the Covaris S2 Focused Ultrasonicator (Covaris, Inc., Woburn, MA). Library construction including end-repairing ...
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bioRxiv - Genomics 2019Quote: ... 5 µg of the genomic DNA extracted from adipose or lung tissues of randomly selected mice (n = 2) were sheared with an S2 ultrasonicator (Covaris, Woburn, MA, USA) in 10 mM Tris buffer (pH 8.0 ...
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bioRxiv - Genomics 2019Quote: ... Extracted genomic DNA was fragmented using a Covaris S220 with the manufacturer’s recommended settings for generating ∼350 base pair length fragments (Covaris, Inc., Woburn, MA 01801). Double sided size selection was performed using KAPA Pure Beads to retain only fragments within the 250-450 base pair range using the manufacturer’s protocol and eluted in 40 μl of TE buffer (Roche Sequencing Solutions ...
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bioRxiv - Genetics 2020Quote: ... a SMRTbell library was constructed from a genomic DNA sample sheared to an average size of ~10 kb using the G-tubes protocol (Covaris, Woburn, MA, USA). Single strands were removed ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: A total of 1 – 10 ng genomic DNA was fragmented by shearing to an average fragment size of 300 bp using an Adaptive Focused Acoustics sonicator (Covaris, Woburn, MA, USA). After purification ...
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bioRxiv - Genomics 2021Quote: DNA (100 ng) was sheared into fragments of approximately 300-400 bp in a Covaris E210 system (96 well format, Covaris, Inc. Woburn, MA) followed by purification of the fragmented DNA using AMPure XP beads ...
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bioRxiv - Genomics 2021Quote: ... STK005 SMRTbell libraries were constructed from genomic DNA samples sheared to between 10 and 20 kb using the G-tubes protocol (Covaris; Woburn, MA, USA), end repaired ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA was fragmented to 100-600 bp with peak size of 350-400 bp using an LE220 Ultrasonicator (Covaris, Woburn, MA, USA). For FS PCR-free libraries ...
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bioRxiv - Genetics 2020Quote: 2 μg of lymphoma DNA was thawed fragmented in 49 μl of Nuclease Free Water (NFW) for 2 minutes at 7,000 revolutions per minute (rpm) using g-TUBE (Covaris®, Woburn, MA, USA) following manufacturer recommendations ...
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bioRxiv - Evolutionary Biology 2022Quote: ... genomic DNA of a female migratory locust was isolated and sheared to an average size of 20 kb using a g-TUBE device (Covaris, Woburn, MA, USA). The sheared DNA was purified and end-repaired using polishing enzymes ...
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bioRxiv - Plant Biology 2022Quote: ... The diluted Hi-C samples were fragmented to a mean size of 300 bp by sonication using a Covaris M220 sonication system (Covaris, Woburn, MA, USA) in a milliTUBE 1 ml AFA Fibre (Covaris) ...
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bioRxiv - Genomics 2023Quote: ... 400 ng of gDNA was brought up to 50 μL volume with 1X low-EDTA TE buffer and sheared with a Covaris E220 sonicator (Covaris, Inc., Woburn, MA) to an average 200 base pare size using the following settings ...
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bioRxiv - Molecular Biology 2023Quote: ... The gRNA was fragmented into approximately 200 nt long fragments using an S220 Focused-ultrasonicator (Duty Cycle 10%; Intensity 5; Peak Incident Power 175 Watts; Cycles per Burst 200; Processing time 200 s; Covaris, Woburn, MA, USA). The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: Control genomic DNA and amplification products were ethanol-precipitated and ∼1 mg of DNA was sheared into ∼350-bp fragments using a Covaris M220 focused acoustic shearer (Covaris, Woburn, MA, USA) following the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... was mechanically fragmented to a target size between 350 and 400 base pairs (bp) on the Covaris LE220 focused ultrasonicator (Covaris, Woburn, MA, USA). The sheared gDNA underwent size selection using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 5 µg of sperm DNA from 4 CT and 4 Cort F0 mice were fragmented by centrifugation for 60 s at 7200 rpm using g-TUBES (Covaris, Woburn, MA, USA) to obtain 8 kb-long fragments ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was fragmented to an average size of 500 bp using a DNA shearing system (M220 focused ultrasonicator; Covaris Inc., MA. USA). A paired-end library was constructed using a TruSeq DNA PCR-Free Library Prep kit (Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or 100 ng genomic DNA spiked with 0.5 ng lambda DNA (for whole genome bisulfite sequencing - WGBS) were fragmented by sonication to 200-300 bp with Covaris S220 (Covaris Inc., Woburn, MA, USA). For WGBS ...
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bioRxiv - Genomics 2020Quote: A minimum of 200-800ng of the pooled PCR products in 55μl were then sheared by ultra-sonication (Covaris LE220-plus series, MA, USA) to generate a target fragment size of 250-750bp using the following parameters ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For each sample the DNA of pooled female and male flies was extracted using a high salt extraction protocol [58] and sheared with a Covaris S2 device (Covaris, Inc. Woburn, MA, USA). Libraries were prepared using the TruSeq DNA PCR-Free protocol (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... DNA-Seq libraries for phylogenic analysis were prepared based on BrAD-seq (42) with the following modifications: After DNA fragmentation with Covaris E220 (Covaris, Inc. Woburn, MA, USA), the fragmented DNA was end-repaired ...
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bioRxiv - Microbiology 2021Quote: ... or 15 kb (for stationary phase samples and bacteroids isolated from dnf mutant nodules and the matched wild-type A17 sample) using g-TUBEs (Covaris Inc., Woburn, MA, USA). Sheared DNA was treated with exonuclease to remove single-stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio ...