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Citations for Covaris :
101 - 144 of 144 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from young fresh leaves and then randomly sheared to fragments with an average size of 20 kb using g-TUBE (Covaris). The sheared DNA was used to construct the PacBio SMRT library following the standard SMRT bell construction protocol (PacBio) ...
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bioRxiv - Microbiology 2021Quote: 2 μg of extracted DNA libraries were resuspended in purified water and sheared to approximately 550 bp fragments using a S220 focussed-ultrasonicator (Covaris), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... The chromatin preparation was then sonicated to fragments enriched in the size range of 150 to 500 bp using S220 Focused Ultrasonicator (Covaris) and centrifuged at 14000 rpm for 10 min at 4 °C to pellet insoluble material ...
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bioRxiv - Microbiology 2022Quote: ... was resuspended in 50 µL distilled water and sheared to approximately 550 bp fragments using a S220 focussed-ultrasonicator (Covaris), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... genomic DNA was adjusted to 10 ng/μL in 150 μL volume and sheared to approximately 10 kb fragments using g-TUBES (Covaris) following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... post-RCA DNA was debranched and fragmented to 6-8 kb-sized fragments using the above-mentioned protocol with the modification of the g-TUBE (Covaris) being centrifuged at 13200 rpm (16363 RCF ...
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bioRxiv - Plant Biology 2022Quote: ... The genomic DNA was sheared into ~20 kb DNA fragments with a g-TUBE Prep Station (Covaris, Woburn, MA, USA) and a HiFi SMRTbell library was constructed with the SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was sonicated to a mean fragment size of 350 bp using a Covaris E220 instrument (Covaris, Woburn, MA) and libraries were generated using the xGen Prism library prep kit for NGS (Integrated DNA Technologies ...
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bioRxiv - Genomics 2023Quote: The genomic DNA was adjusted to 10 ng/uL in 150 µL and sheared to approximately 10 kilobase fragments using g-TUBES (Covaris) following the manufacturer’s instructios. ...
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bioRxiv - Plant Biology 2023Quote: ... Young leaves from a single 3-week-old plant were collected for high-molecular-weight DNA extraction followed by shearing to fragments with an average size of 20 kb using g-TUBE (Covaris). The sheared DNA was then used to construct a PacBio SMRT library following the standard SMRT bell construction protocol ...
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bioRxiv - Molecular Biology 2022Quote: Chromatin was extracted using truChIP Chromatin Shearing Kit with formaldehyde and prepared according to manufacturer’s Low Cell protocol (Covaris PN010179 Rev O, Dec 2020). 11.1% formaldehyde was made fresh from 16% concentrated formaldehyde ...
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bioRxiv - Cancer Biology 2021Quote: ... One µg of genomic DNA was sheared to a mean fragment length of about 220 bp using focused acoustic energy (Covaris E220). The size distribution of the fragmented sample was determined by using the Caliper LabChip GX system ...
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bioRxiv - Microbiology 2019Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro-TUBE according to manufacturer’s recommended settings (Covaris, Woburn, MA). Sheared DNA was purified with 1x SPRIselect beads according to manufacturer’s instructions (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 cycles per burst for 30 min at 4° C to obtain 100-500 base pair chromatin fragments (Covaris, Woburn, CA). Nuclear sheared lysates were clarified by centrifugation at 15,000 x g for 10 min at 4° C ...
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bioRxiv - Genetics 2021Quote: ... SF/CamEiJ and SKIVE/EiJ mice was sheared into fragments of approximately 200 bp with the Covaris E220 system (Covaris, USA). The sheared DNA fragments were then purified for each of the 16 mice strains using AMPure XP Beads (Beckman ...
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bioRxiv - Microbiology 2021Quote: ... and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro- TUBE according to the manufacturer’s recommended settings (Covaris, Woburn, MA). HIV-1 insertion site libraries were prepared and sequenced using methods described previously (44) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg of DNA per sample was sheared to the average fragment size of 160 bp using a Covaris S220 sonicator (Covaris, #500217). DNA quality was validated using the TapeStation 4200 system (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... Extracted chromatin was sheared in SDS lysis buffer (200 μl 1× 109 trophozoites and 5×108 gametocytes) to obtain a fragment size of 100-150bp using an M220 focused-ultrasonicator (Covaris Inc.) using the following settings ...
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bioRxiv - Microbiology 2020Quote: ... and 500 ng of input DNA was sheared into ∼550 bp fragments using a LE220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Library preparation was performed using the TruSeq PCR-free Library Prep Kit (Illumina) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Samples with DNA fragment lengths longer than 500 base pairs (bp) were sonicated on a Covaris M220 (Covaris, Wobum, MA, USA) for 45 seconds at 50 W peak power and a duty factor of 20% ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was sheared to an average fragment size of 400 bp using a Covaris M220 ultrasonicator (Covaris, Woburn, MA, USA). Libraries were prepared using a protocol largely based on (Rowan et al. ...
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bioRxiv - Genetics 2019Quote: ... One µg of DNA from each was fragmented to 250-bp fragments by adjusting the treatment time to 85 sec using a Covaris S220 sonicator (Covaris Inc.). Libraries were prepared using a Nextera DNA Sample Prep Kit and Bioo Scientific NEXTflex DNA Barcodes ...
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bioRxiv - Cancer Biology 2023Quote: ... We utilized 20-50ng of cfDNA and 100ng of matched germline DNA (gDNA) that was sonicated to reduce the fragment size to 180-220bp (Covaris M220). Libraries were sequenced on the Illumina HiSeq2500 platform by the Next Generation Sequencing Facility at the University of Trento (Italy) ...
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bioRxiv - Systems Biology 2023Quote: ... the extracted genomic DNA was digested into 400–550 bp fragments by using the M220 Focused-ultrasonicator (Covaris Ltd, Brighton, UK). The sequencing library was constructed using the Nextera XT DNA Library LT kit (Illumina ...
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bioRxiv - Genomics 2023Quote: ... We quantified the purified DNA with a Qubit instrument (Thermo Fischer Scientific) to shear 330 ng of DNA into fragments of ∼ 300 bp with a Covaris sonicator (Covaris Inc.) and ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Half of the DNA was then sheared into 20 kb fragments with a COVARIS g-TUBE (Covaris, Inc., Woburn, MA, USA), centrifuging at 4,200 rpm for 1 minute to increase DNA throughput ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was sheared to an average fragment size of 350 bp with a Covaris M220 Focused-ultrasonicator (Covaris, Woburn, Massachusetts, USA). We assessed the DNA fragment size distribution using a High Sensitivity DNA ScreenTape on a 4150 TapeStation System (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was resuspended in water and sheared into 3-5 kb fragments using the S220 Focused-ultrasonicator (Covaris Inc., MA, USA).
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bioRxiv - Genomics 2020Quote: ... The DNA was sheared to a mean fragment length of 20-30 kb with Covaris g-TUBEs (Covaris Inc., Woburn, MA, USA). The length of the DNA was measured with a TapeStation (4200 ...
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bioRxiv - Genomics 2019Quote: ... and genomic DNA from GM12878 lymphoblast cell line (Coriell Institute) were first sheared to an average fragment size of 8 kb using g-tubes (Covaris Cat. 520079). The fragmented DNA was PCR amplified to generate unmethylated DNA using the first steps of low input ligation kit SQK-LWP001 (ONT) ...
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bioRxiv - Genomics 2019Quote: ... with the following changes: 1 μg of gDNA was sheared to 70-90bp fragments using the Covaris S220 (Covaris Inc., Woburn, MA) and 150bp fragment libraries were constructed according to standard SOLiD Fragment library protocols (Applied Biosystems ...
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bioRxiv - Physiology 2021Quote: ... The resuspended chromatin was sonicated into fragments (200 to 500 bps long) at 4 C using an ultrasonic crusher (Covaris, Massachusetts, USA). The sheared chromatin was immunoprecipitated with anti-HA antibodies (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... For the paired end library a total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
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bioRxiv - Neuroscience 2024Quote: ... was taken to the Duke University Center for Genomic and Computational Biology (GCB) and sheared to achieve 175 bp-long fragments using Covaris S220 (Covaris, Woburn, MA). DNA quality was assessed using Agilent 4200 TapeStation ...
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bioRxiv - Genomics 2019Quote: ... we prepared the library using 10 μg genomic DNA that was sheared to a size of 20kb fragments by g-tubes (Covaris, Inc., Woburn, MA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... DNA (10 ng to 500 ng) was sheared into fragments of approximately 300-400 bp in a Covaris E210 system (96 well format, Covaris, Inc. Woburn, MA) followed by purification of the fragmented DNA using AMPure XP beads ...
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bioRxiv - Genomics 2019Quote: ... 100-ng of genomic DNA from each sample was sheared to fragment sizes ranging from 300∼500-kb by the Covaris S2 Focused Ultrasonicator (Covaris, Inc., Woburn, MA). Library construction including end-repairing ...
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bioRxiv - Genomics 2019Quote: ... Extracted genomic DNA was fragmented using a Covaris S220 with the manufacturer’s recommended settings for generating ∼350 base pair length fragments (Covaris, Inc., Woburn, MA 01801). Double sided size selection was performed using KAPA Pure Beads to retain only fragments within the 250-450 base pair range using the manufacturer’s protocol and eluted in 40 μl of TE buffer (Roche Sequencing Solutions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: A total of 1 – 10 ng genomic DNA was fragmented by shearing to an average fragment size of 300 bp using an Adaptive Focused Acoustics sonicator (Covaris, Woburn, MA, USA). After purification ...
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bioRxiv - Genomics 2021Quote: DNA (100 ng) was sheared into fragments of approximately 300-400 bp in a Covaris E210 system (96 well format, Covaris, Inc. Woburn, MA) followed by purification of the fragmented DNA using AMPure XP beads ...
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bioRxiv - Molecular Biology 2023Quote: Control genomic DNA and amplification products were ethanol-precipitated and ∼1 mg of DNA was sheared into ∼350-bp fragments using a Covaris M220 focused acoustic shearer (Covaris, Woburn, MA, USA) following the manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA was fragmented into fragments ranging from 400 to 550 base pairs (bp) through the utilization of the M220 focused-ultrasonicator (Covaris Ltd. Brighton, UK). The sequencing library was prepared with the TruSeq DNA Library LT kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... 2 µg of DNA for each pool was sheared to an average fragment size of 340 base pairs using a Covaris S220 focused-ultrasonicator (Covaris, Inc., Woburn, Massachusetts, USA). The samples were then cleaned using DNAClean XP (Beckman Coulter Life Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplified DNA fragments (100 ng) were sheared to the average length of 250 bp by using an ultrasonicator (Covaris focused-ultrasonicator S220; Covaris) and purified by using magnetic beads (Agencourt AMPure XP ...