Labshake search
Citations for Covaris :
51 - 100 of 136 citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... a minimum of 630 ng up to 2 ug of library were sonicated by Covaris E220 ultrasonicator at the following conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA (2 µg) was fragmented to an average of 500 bp by sonication (Covaris) and ligation of full-length barcoded Illumina adapters was performed using the TruSeq DNA PCR-free kit (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue samples were powderized with 2 pulses (at maximum intensity) using a CryoPrepTM system (Covaris). Equal amounts of powder were transferred to a pre-cooled 2 mL tube ...
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bioRxiv - Genetics 2019Quote: ... Cells were resuspended in 1 ml shearing buffer (10 mM Tris pH 8.0, 5 mM EDTA, 0.1% SDS) and sonicated (Covaris) with the following settings (Peak Incident Power = 100 W ...
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 2 ml shearing buffer and sonicated using a Focused Ultrasonicator M220 (Covaris). Sonication was performed in two rounds using two different sonication conditions ...
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bioRxiv - Genomics 2022Quote: ... 1-2 micrograms of high molecular weight DNA was sheared using g-tube (Covaris, Woburn, MA) to 5-9 Kbp at a 7000 RPM and size selected using the 0.75% DF 3-10kb Marker S1-Improved Recovery cassette definition on the Blue Pippin (Sage Science) ...
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bioRxiv - Microbiology 2022Quote: ... 2 µg of genomic DNA was fragmented using an ultra-focused sonicator (Covaris M220, Covaris, Inc.) in microTUBE-50 AFA Fiber Screw-Cap tubes ...
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bioRxiv - Microbiology 2022Quote: ... 2 µg of genomic DNA was fragmented using an ultra-focused sonicator (Covaris M220, Covaris, Inc.) in microTUBE-50 AFA Fiber Screw-Cap tubes ...
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bioRxiv - Microbiology 2024Quote: ... DNA was fragmented into 2–8 kb pieces using focused ultrasonication (Covaris E220 with miniTUBE red). DNA Fragments were subsequently cloned into a PCR-linearized pZE21 expression vector (primers 1 and 2 ...
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bioRxiv - Genetics 2020Quote: The TruChIP tissue shearing kit (Covaris, Woburn, MA) was used to process 80 mg of mouse liver per sample ...
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bioRxiv - Immunology 2023Quote: ... In brief: Blood plasma was transferred to 2 mL AFA glass tubes (Covaris Inc, Woburn, Massachusetts, USA) and mixed with ultra-pure water ...
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bioRxiv - Genomics 2019Quote: ... DNA was then resuspended in 10 mM T ris-HCl pH 8 and 5 ug aliquots were sonicated by Covaris S2 to fragment sizes in the range of 450-550 bp ...
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bioRxiv - Neuroscience 2023Quote: ... (100 µL per well) and sonicated for 5 min using the Covaris FPPE protocol using the R230 Focused Ultrasonication Device from Covaris (peak power ...
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bioRxiv - Genomics 2021Quote: Illumina libraries were generated by the Norwegian Sequencing Centre using the following protocol: 1 µg of genomic DNA was sheared using 96 microTUBE-50 AFA Fiber plates (Covaris Inc., Woburn, MA, USA) on a Covaris E220 system (Covaris Inc. ...
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bioRxiv - Genomics 2022Quote: ... and the Covaris truChIP Chromatin shearing kit (Covaris; 520154), according to the manufacturers’ instructions ...
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bioRxiv - Genomics 2021Quote: ... 2 µg of each gDNA were sheared using a Covaris M220 Focused-ultrasonicator (Covaris, Inc. Woburn, MA, USA), end-repaired ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA (2□μg) was diluted in 200 μL of elution buffer and fragmented using g-TUBE (Covaris) by centrifuging at 2400×g for 2□min ...
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bioRxiv - Microbiology 2020Quote: ... Extracted chromatin was sheared in SDS lysis buffer (200 μl 1× 109 trophozoites and 5×108 gametocytes) to obtain a fragment size of 100-150bp using an M220 focused-ultrasonicator (Covaris Inc.) using the following settings ...
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bioRxiv - Microbiology 2021Quote: ... DNA was sheared to an average length of approximately 5-10 kb (genomic DNA) using g-TUBEs (Covaris, Woburn, MA, USA) and SMRTbell template sequencing libraries were prepared using sheared DNA ...
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bioRxiv - Microbiology 2023Quote: ... were subjected to soni cation using the Covaris S220 (Power = 140, Duty = 5, Cycles/Burst = 200) for 18 minutes with microTUBE 500 AFA tubes (Covaris 520185) and each sample was incubated with 5 µg anti-FLAG M2 antibody (Millipore A2220 ...
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bioRxiv - Genetics 2022Quote: ... Cell nuclei were resuspended in 2 mL 0.4% SDS shearing buffer for sonication with a Covaris S220X sonicator (Covaris) for 7 min ...
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bioRxiv - Genetics 2019Quote: ... one frozen 2 mm core was pulverized in the pulverization bag using the Covaris CryoPrep system (Covaris, Woburn, MA) once at intensity 3 and once at intensity 4 ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... 50 mg of deep-frozen feces sample were transferred into 2 mL AFA glass tubes (Covaris Inc, Woburn, USA) and mixed with 400 μL of ultrapure methanol and 800 μL of MTBE (solvent grade) ...
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bioRxiv - Genomics 2021Quote: ... we used truChIP Chromatin Shearing Kit (Covaris Inc., MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Chromatin was prepared using truChIP Chromatin Shearing Tissue Kit (Covaris) according to the manufacture’s protocol with modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were prepared using truChIP Chromatin Shearing Reagent Kit (Covaris).
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (5-10 µg) samples were sheared to an average size of 20 kbp via G-tube (Covaris; Woburn, MA, USA), end repaired and ligated to hairpin barcoded adapters prior to sequencing ...
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bioRxiv - Genomics 2024Quote: ... a dilution of 5 µg HMW DNA in 120 µL elution buffer was transferred to a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation with 1,990 x g for 2 min ...
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bioRxiv - Genetics 2024Quote: ... of purified DNA were fragmented to ∼350bp using the Covaris S2 system (10% duty cycle, intensity 5 for 2x 45 sec in Covaris AFA tubes) followed by a concentration step with Zymo columns (DNA Clean & Concentrator) ...
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bioRxiv - Genomics 2020Quote: ... A total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
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bioRxiv - Microbiology 2022Quote: ... ∼2 μg of genomic DNA was fragmented to a size of ∼300 base-pairs using a Covaris Focused ultrasonicator (Covaris), followed by end repair using the NEBNext End Repair module (NEB) ...
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bioRxiv - Genomics 2020Quote: ... Chromatin shearing was performed using truChIP Chromatin Shearing Reagent Kit (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Using the truChIP High Cell Chromatin Shearing kit (Covaris, United States), cells were prepared for sonication according to the Covaris protocol ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The gRNA was fragmented into approximately 200 nt long fragments using an S220 Focused-ultrasonicator (Duty Cycle 10%; Intensity 5; Peak Incident Power 175 Watts; Cycles per Burst 200; Processing time 200 s; Covaris, Woburn, MA, USA). The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: DNA shearing was first performed from 5 µg HMW DNA in 120 µL elution buffer using a g-tube (Covaris Part No. 520079) with 6 passes of centrifugation at 1,990 x g for 2 min ...
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bioRxiv - Genomics 2020Quote: ... Shearing of chromatin was performed using truChIP Chromatin Shearing Reagent Kit (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin was prepared with truChIP™ Chromatin Shearing Reagent Kit (Covaris 520154) and sheared with Covaris S220 according to the kit manual ...
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bioRxiv - Cancer Biology 2021Quote: ... Shearing of chromatin was performed using truChIP Chromatin Shearing Reagent Kit (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Shearing of chromatin was performed using truChIP Chromatin Shearing Reagent Kit (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Chromatin sonication was performed by using the truChIP Chromatin Shearing Kit (Covaris) with Focused-Ultrasonicator M220 (Corvaris ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were fixed and harvested using the truChIP chromatin shearing kit (Covaris) and IP was conducted according to Richard Myers lab ChIP-seq protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and for the clinical samples the truXTRAC FFPE kit was used (Covaris). DNase treatment was performed using the Turbo DNase kit (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... and processed for ChIP using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 50 mg of deep-frozen feces sample were transferred into 2 ml adaptive focused acoustics (AFA) glass tubes (Covaris Inc, Woburn, USA) and mixed with 400 μl of ultrapure methanol and 800 μL of Methyl ter-butyl ether (MTBE ...
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bioRxiv - Genomics 2022Quote: ... For the paired end library a total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
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bioRxiv - Genomics 2019Quote: ... 5 µg of the genomic DNA extracted from adipose or lung tissues of randomly selected mice (n = 2) were sheared with an S2 ultrasonicator (Covaris, Woburn, MA, USA) in 10 mM Tris buffer (pH 8.0 ...
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bioRxiv - Genetics 2020Quote: 2 μg of lymphoma DNA was thawed fragmented in 49 μl of Nuclease Free Water (NFW) for 2 minutes at 7,000 revolutions per minute (rpm) using g-TUBE (Covaris®, Woburn, MA, USA) following manufacturer recommendations ...
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bioRxiv - Cancer Biology 2020Quote: Chromatin was prepared with the truChIP™ Chromatin Shearing Kit (Covaris, Brighton, UK) according to manufacturer’s instructions ...