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1 - 50 of 1458 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020

• SNRK regulates TGFβ levels in atria to control cardiac fibrosis

  Karthikeyan Thirugnanam, et al., bioRxiv - Cell Biology 2024

  Quote: ... Smad 2/3 (Novus Biologicals, Cat# AF3797, 1:200 dilution), GAPDH (Santa Cruz ...
• Enteropathogenic Escherichia coli (EPEC) Infection Induces Diarrhea, Intestinal Damage, Metabolic Alterations and Increased Intestinal Permeability in a Murine Model

  Solanka E. Ledwaba, et al., bioRxiv - Microbiology 2020

  Quote: ... 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600; rabbit anti-claudin-1, 1:1000, Novus biological, NBP1-77036) and secondary antibodies (Cy3-conjugated AffiniPure donkey anti-rabbit ...
• The hepatokine FGL1 regulates hepcidin and iron metabolism during the recovery from hemorrhage-induced anemia in mice

  Ugo Sardo, et al., bioRxiv - Physiology 2023

  Quote: ... Incubation with primary antibody was followed by 3 washes and membranes were incubated 2 hours with goat anti-Human IgG (Novus biological, NBP1-75006, 1/10000), goat anti-rabbit IgG (Cell signaling ...
• Stabilization of GTSE1 by cyclin D1-CDK4/6 promotes cell proliferation: relevance in cancer prognosis

  Nelson García-Vázquez, et al., bioRxiv - Biochemistry 2024

  Quote: ... LC-3 (1:5,000, Novus Biological NB100–2220), p21 (1:1,000 ...
• Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation

  Allison K. Meyers, et al., bioRxiv - Immunology 2021

  Quote: ... LC-3 (Novus Biologicals, no. NB100-2220; 1:500), P62 (Novus Biologicals ...
• Met/HGFR triggers detrimental reactive microglia in TBI

  Rida Rehman, et al., bioRxiv - Neuroscience 2021

  Quote: ... Mouse anti-Integrin beta 3 (1:50, Novus biologicals), Guinea pig anti-Iba1 (1:500 ...
• Disruption of augmin-mediated microtubule nucleation in neural stem cells causes p53-dependent apoptosis and aborts brain development

  Ricardo Viais, et al., bioRxiv - Cell Biology 2020

  Quote: ... cleaved caspase-3 (Novus Biologicals #MAB835, dilution 1:500), γ-tubulin (ExBio ...
• TFAP2C and HNRNPK control mTOR cell metabolism and prion propagation

  Stefano Sellitto, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-Caspase 3 1:1000 (Novus Biologicals, NB100-56708), anti-GPX4 1:1000 (ab125066) ...
• 3D microarchitecture of the human tuberculous granuloma

  Gordon Wells, et al., bioRxiv - Microbiology 2020

  Quote: ... 3-mercaptopyruvate sulfurtransferase (MPST; NBP1-82617, Novus Biologicals, 1:100), CD68 (M0814-CD68-KP1,DAKO,1:3000) ...
• Condensate formation of the human RNA-binding protein SMAUG1 is controlled by its intrinsically disordered regions and interactions with 14-3-3 proteins

  John Fehilly, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-14-3-3ζ (Novus Biologicals, NBP2-24593SS, 1:1000). Following primary antibody incubation ...
• Cerebral Organoids In Primary Progressive Multiple Sclerosis Reveal Stem Cell Disruption And Failure To Produce Oligodendrocytes

  Nicolas Daviaud, et al., bioRxiv - Neuroscience 2022

  Quote: ... Slides were then incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: rabbit anti-cleaved caspase 3 (1:200, Novus Biologicals), rat anti-CTIP2 (1:500 ...
• Mimicking the breast metastatic microenvironment: characterization of a novel syngeneic model of HER2⁺ breast cancer

  Aaron G. Baugh, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-AE-1/AE-3 (1:200 dilution; Novus Biologicals, cat. NBP2-29429). Slides were then incubated with HRP-conjugated anti-rabbit secondary antibody (Leica Microsystems ...
• Shared and divergent phase separation and aggregation properties of brain-expressed ubiquilins

  Julia E. Gerson, et al., bioRxiv - Neuroscience 2020

  Quote: ... Membranes were then probed overnight at 4℃ in anti-Ubiquilin-2 (Novus Biologicals, cat. #NBP2-25164; 1:2000), anti-Ubiquilin-1 (Novus biologicals ...
• RbAp46/48^LIN-53 and HAT-1 are required for initial CENP-A^HCP-3 deposition and de novo centromere formation in Caenorhabditis elegans embryos

  Zhongyang Lin, Karen Wing Yee Yuen, bioRxiv - Cell Biology 2020

  Quote: ... using rabbit (Rb)-anti-HCP-3 (1:1000; Novus biologicals 29540002), Rb-anti-KNL-2 (1:500 ...
• Inflammation-mediated Upregulation of VCAM-1 but not KIM-1 during Acute Kidney Injury to Chronic Kidney Disease Transition

  Isabel Melchinger, et al., bioRxiv - Pathology 2022

  Quote: ... Membrane was blocked with 5% non-fat milk in TBST for 2 hrs and probed overnight at 4°C with the primary antibody against TIM-1/KIM-1/HAVCR (#AF1817, Novus Biologicals). After washing the membrane with TBST ...
• Skp1 proteins are structural components of the synaptonemal complex in C. elegans

  Joshua Blundon, et al., bioRxiv - Cell Biology 2023

  Quote: ... and anti-HIM-3 (rabbit polyclonal, 1:200 or 1:100; Novus Biologicals #53470002) or anti HTP-3 (chicken polyclonal ...
• Myosin-1b interacts with UNC45A and controls intestinal epithelial morphogenesis

  Céline Revenu, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Sections were incubated for 30 min at room temperature in blocking buffer (3% BSA in PBS) and then overnight at 4°C with anti Myo1b antibody (1:200, Novus Biologicals NBP1-87739) in blocking ...
• Python nidoviruses, more than respiratory pathogens

  Eva Dervas, et al., bioRxiv - Pathology 2020

  Quote: ... followed by a 15 min incubation with DAPI (4′, 6-diamidino-2-phenylindole, Novus Biologicals; 1:10,000 in PBS). Sections were washed twice with distilled water ...
• Differentiation of Peritubular Myoid-Like Cells from Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... 3 Beta- And Steroid Delta-Isomerase 1 (HSD3β, Novus Biologicals, NB110-7844) 1:200 ...
• A Novel Organoid Model of In Vitro Spermatogenesis Using Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... 3 Beta- And Steroid Delta-Isomerase 1 (HSD3β, Novus Biologicals, NB110-7844) 1:200 ...
• Microfluidic bioprinting for the in vitro generation of novel biomimetic human testicular tissues

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... 3 Beta- And Steroid Delta-Isomerase 1 (HSD3β, Novus Biologicals, NB110-7844) 1:200 ...
• Human TRMT2A methylates tRNA and contributes to translation fidelity

  Monika Witzenberger, et al., bioRxiv - Biochemistry 2023

  Quote: ... Primary antibody used: mouse anti-BirA (1:10; Novus Biologicals, #5B11c3-3). Secondary antibody used ...
• HDAC1 SUMOylation promotes Argonaute directed transcriptional silencing in C. elegans

  Heesun Kim, et al., bioRxiv - Molecular Biology 2020

  Quote: ... anti-HPL-2 (1:1000) (Novus Biologicals, 38630002), anti-LIN-53 (1:1000 ...
• Microfluidic organotypic device to test intestinal mucosal barrier permeability ex vivo

  Amanda E Cherwin, et al., bioRxiv - Bioengineering 2023

  Quote: ... 1:500 and anti-MUC-2 (Novus Biologicals) at 3 μg/mL ...
• GASOTRANSMITTER MODULATION OF HYPOGLOSSAL MOTONEURON ACTIVITY

  Brigitte Browe, et al., bioRxiv - Neuroscience 2022

  Quote: ... HO-2 (1:200, Novus Biologicals; # NBP1-52849) and CSE (1:250 ...
• Misaligned chromosomes that satisfy the spindle assembly checkpoint are a strong predictor of micronuclei formation in dividing cancer cells

  Ana Margarida Gomes, et al., bioRxiv - Cell Biology 2022

  Quote: ... rabbit anti-Septin-2 (1:500; Novus Biologicals), rabbit anti-CEP90/PIBF1 (1:1000 ...
• SARS-CoV-2 Omicron Envelope T9I adaptation confers resistance to autophagy

  Susanne Klute, et al., bioRxiv - Microbiology 2024

  Quote: ... the cells were stained with the following primary antibodies diluted in DPBS with 1% FBS for 2 h at 4 °C: Monoclonal mouse anti-StrepII-tag (1:200; Novus Biologicals, #NBP2-43735), monoclonal rabbit anti-LAMP1 (1:200 ...
• Morphometric Analysis of the Thymic Epithelial Cell (TEC) Network Using Integrated and Orthogonal Digital Pathology Approaches

  Maria K. Lagou, et al., bioRxiv - Immunology 2024

  Quote: ... Nuclear counterstaining was performed for 5min with 4′,6-diamidino-2-phenylindole (DAPI, dilution 1:1000 in PBST 0,05%; Novus Biologicals), or with Deep Red Anthraquinone 5 (DRAQ5 ...
• Disrupting ACE2 Dimerization Mitigates the Infection by SARS-COV-2

  Jiaqi Zhu, Yue Su, Young Tang, bioRxiv - Molecular Biology 2022

  Quote: ... The membrane was blocked by 5% skim milk and incubated with ACE-2 Antibody (1:2,000, Novus Biologicals, CO, USA), 6xHN Polyclonal Antibody (1:2000 ...
• Nose-only Exposure to Cherry and Tobacco Flavored E-cigarettes Induced Lung Inflammation in Mice in a Sex-dependent Manner

  Thomas Lamb, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... Ly6B.2 (Novus Biological Cat # NBP2-13077, 1:250), CD4 (Invitrogen Cat # 25-0041-82 ...
• Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure

  Sarah R. Barger, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1 μg/mL rabbit α-LEM-2 (Novus Biologicals), 1 μg/mL rabbit α-CHMP-7 (Shankar et al. ...
• The distance between the plasma membrane and the actomyosin cortex acts as a nanogate to control cell surface mechanics

  Sergio Lembo, et al., bioRxiv - Biophysics 2023

  Quote: ... The blot was blocked in 5% BSA in TBST and subsequently incubated with primary antibody (anti-mDia1, #20624-1-AP, Thermo Fischer Scientific, 1:1000, overnight at 4° C; GAPDH, #NB300-221, Novus Biologicals, 1:40.000, 1-2 hrs at RT). Secondary antibody staining was performed for 1 hour at RT in blocking solution (Donkey-Anti-Rabbit-HRP ...
• APOE4 alters the lipid droplet proteome and modulates droplet dynamics

  Cassi M. Friday, et al., bioRxiv - Physiology 2024

  Quote: ... The membranes were incubated overnight at 4° Celsius in 1:1000 PLIN-2 primary antibody solution (Novus Biologicals, Centennial, CO, USA). After incubation ...
• CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression

  Désirée Schatton, et al., bioRxiv - Cell Biology 2021

  Quote: ... 300-500 µg of protein were diluted in 250 µl IP buffer and incubated for 3h in head-to-toe agitation at 4°C with 0.5 µg of the specific antibody: rabbit polyclonal rabbit anti-CLUH [#NB100-93305 (1) and #NB100-93306 (2) from Novus Biologicals] ...
• SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta

  Lissenya B. Argueta, et al., bioRxiv - Cell Biology 2021

  Quote: ... for 1 hour at room temperature and then incubated overnight at 4°C in a humid chamber with primary antibodies (SARS-CoV-2-N, GeneTex GTX635679, at 1:100; CD163,Novus Biologicals NBP2-48846, 1:250) diluted in 1% BSA/0.1% Triton-X PBS (PBST) ...
• Cross-species proteomic and microRNA comparison of extracellular vesicles in human milk, cow’s milk, and infant formula products: moving towards next generation infant formula products

  Natalie P. Turner, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Rabbit polyclonal (Novus Biologicals (Invitro technologies), Melbourne ...
• Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9

  Chao Wang, et al., bioRxiv - Immunology 2024

  Quote: ... and mouse anti-pan-cytokeratin (Novus Biologicals, AE-1/AE-3, Cat# NBP2-29429). Finally ...
• Damage-induced IL-18 stimulates thymic NK Cells limiting endogenous tissue regeneration

  David Granadier, et al., bioRxiv - Immunology 2024

  Quote: ... The resulting supernatant was quantified using cytokine specific ELISA kits (IL-1β Invitrogen #88-7013-22; IL-18 Thermo Fisher #BMS618-3; GzmB R&D #DY1865; Perforin Novus Biologicals # NBP3-00452 ...
• Shared and divergent phase separation and aggregation properties of brain-expressed ubiquilins

  Julia E. Gerson, et al., bioRxiv - Neuroscience 2020

  Quote: ... anti-Ubiquilin-2 (Novus Biologicals, cat. #NBP2-25164; 1:2000), anti-Ubiquilin-1 (Novus biologicals ...
• A Cytoskeletal Function for PBRM1 Reading Methylated Microtubules

  Menuka Karki, et al., bioRxiv - Cell Biology 2020

  Quote: ... and ARID2 (Novus biologicals, # NBP-2-57220, 1:250 IF). Secondary antibodies (1:5,000 ...
• OTUD1 deubiquitylase regulates NF-κB- and KEAP1-mediated inflammatory responses and reactive oxygen species-associated cell death pathways

  Daisuke Oikawa, et al., bioRxiv - Cell Biology 2021

  Quote: ... and OTUD1 (NBP1-90484, Novus Biologicals; 3 μg) were used.
• Single-nucleus and spatial transcriptomics of archival pancreatic cancer reveals multi-compartment reprogramming after neoadjuvant treatment

  William L. Hwang, et al., bioRxiv - Genomics 2020

  Quote: ... 1:40 anti-panCK-Alexa Fluor 532 (clone AE-1/AE-3; Novus Biologicals, cat. no. NBP2-33200AF532), 1:40 anti-CD45-Alexa Fluor 594 (clone 2B11 + PD7/26 ...
• Enhanced Complement Expression in the Tumor Microenvironment Following Neoadjuvant Therapy: Implications for Immunomodulation and Survival in Pancreatic Ductal Adenocarcinoma

  Xiaofei Zhang, et al., bioRxiv - Genomics 2023

  Quote: ... 1:20 anti-panCK-Alexa Fluor 532 (clone AE-1/AE-3; Novus Biologicals, cat. no. NBP2-33200AF532), and 1:100 anti-αSMA-Alexa Fluor 647 (clone 1A4 ...
• Neuroretinal-derived caveolin-1 promotes endotoxin-induced inflammation in the murine retina

  Jami M. Gurley, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit polyclonal anti-TRAF3 (TNF Receptor-Associated Factor 3; 1:500; Novus Biologicals, #NBP-88639), and rabbit polyclonal anti-Gαt (Transducin ...
• Genetic control of neuronal activity enhances axonal growth only on permissive substrates

  Francina Mesquida-Veny, et al., bioRxiv - Neuroscience 2022

  Quote: ... followed by 3 O/N of primary antibody incubation (SCG-10 (1:1000, Novus Biologicals) in 2% BSA ...
• Intra-axonal translation of Khsrp mRNA slows axon regeneration by destabilizing localized mRNAs

  Priyanka Patel, et al., bioRxiv - Neuroscience 2020

  Quote: ... rabbit anti-Stathmin-2/SCG10 (1:500; Novus Biologicals, NBP1-49461). Stathmin-2/SCG10 immunofluorescence was used to detect regenerating mouse sciatic nerve axons (Shin et al. ...
• The microbiota promotes social behavior by modulating microglial remodeling of forebrain neurons

  Joseph J. Bruckner, et al., bioRxiv - Neuroscience 2022

  Quote: ... and rabbit anti-mCherry (1:100; Novus Biologicals Cat #2-25157). The following secondary antibodies were used ...
• Whole-brain mapping of monosynaptic inputs to midbrain cholinergic neurons

  Icnelia Huerta-Ocampo, et al., bioRxiv - Neuroscience 2020

  Quote: ... and rabbit tryptophan hydroxylase 2 (1:500, Novus Biological, NB100-7455), respectively ...
• Synaptopodin is required for efficient intercellular spread by bacterial pathogens

  Jenna M. Vickery, et al., bioRxiv - Microbiology 2024

  Quote: ... or 1:50 goat anti-Arp 2 (Novus Biologicals, NB100-1037) overnight at 4°C ...
• MicroRNA-22, Specificity protein-1 and Cystathionine β-synthase in early onset Preeclampsia: significance in trophoblast invasion

  Pallavi Arora, et al., bioRxiv - Developmental Biology 2023

  Quote: ... TIMP-2 (Novus Biologicals) at a dilution of 1:200 ...