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Citations for Novus Biologicals :
1501 - 1520 of 1520 citations for Anti CD25 SAP mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Proteins were eluted using Laemmli buffer and analyzed by western blot using Goat anti-Human IgG Fc fragment Secondary Antibody [HRP] (Novus biological NBP1-75006) or anti-BMP6 antibody (R&D systems ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were permeabilized using PBS containing 0.1% saponin for 30 min and blocked using PBS with 1% BSA and 1% goat serum for 1 h before labeling with 1:100 dilution of rabbit polyclonal anti-TGN-46 antibody (Novus biologicals, NBP1-49643). After primary antibody labeling ...
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bioRxiv - Molecular Biology 2024Quote: ... 250ug of lysate was precleared with 20 μl of packed Protein A Sepharose CL-4B resin (Cytivia, 17078001) for 2 hours at 4 °C and then incubated overnight with 3ug of anti-NPM1 antibody (Novus Biologicals, NB600-1030) at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... After 1 h blocking with 3% BSA in tween-tris buffered solution (TTBS) the proteins were detected with the antibody anti-CXCR7/RDC-1 (1:500) (Novus Biologicals, Centennial, USA) labeled with IRDyes and was incubated for 90min at room temperature in 3% BSA in TTBS ...
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bioRxiv - Neuroscience 2021Quote: ... 3 x 5 minutes and then processed according to the technique of immunofluorescence labeling with overnight incubation at 4 °C with the polyclonal anti-Iba1 1:250 (# NB100-1028, Novus Biologicals, Littleton, Colorado, USA), monoclonal anti-GFAP 1:500 (# MAB3402X ...
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bioRxiv - Immunology 2020Quote: ... The EVs pellet suspended in RIPA was used to perform western blot to check the expression of ESAT-6 (anti-ESAT-6 antibody (#ab26246, AbcamInc, USA) and CD63 (#NBP2-42225SS, Novus Biologicals, Centennial, CO, USA).
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bioRxiv - Cell Biology 2022Quote: ... Sections were then incubated in the following primary antisera overnight at 4°C in 1 X PBS + 1% tween + 20% Blocking One solution: rabbit anti-Aldh1a3 (Novus Biologicals; Littleton, CO, USA). Sections were then washed twice with PBS and incubated for 2 h at room temperature with species specific Cy2 and Cy3 conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... 60 µm thick vibratome sections were then immunostained with antibodies against Choline Acetyl-Transferase (ChAT) (anti-ChAT, Novus Biologicals, goat, NBP1-30052, 1:10) and HA (anti-HA ...
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bioRxiv - Immunology 2024Quote: ... and then stained with anti-Rb antibodies and their corresponding secondary IgG (#MA5-11387 Thermo Fisher Scientific, Waltham, MA, #NB100-56598 Novus Biologicals, Centennial, CO).
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bioRxiv - Developmental Biology 2021Quote: ... permeabilized gonads were subsequently incubated with primary antibodies overnight at 4°C [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), rabbit anti-V5 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 5% powdered milk + PBS-T with primary antibodies [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), mouse anti-V5 (1:3000 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody binding to the column was performed using 75 mg of either a mock antibody (donkey anti-goat IgG) as a control or ANP32A antibody (Novus Biologicals, NBP1-97576, clone RJ1). After antibody immobilization ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10 μg of total proteins were loaded for each sample on the Invitrogen 4-12% Bis-Tris gel and the following antibodies were used for immunoblot detection: rabbit anti-Synj1 (Novus Biologicals, NBP1-87842, 1:1000), rabbit anti-GFAP (Abclonal ...
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bioRxiv - Physiology 2023Quote: ... Incubation with primary antibody was followed by 3 washes and membranes were incubated 2 hours with goat anti-Human IgG (Novus biological, NBP1-75006, 1/10000), goat anti-rabbit IgG (Cell signaling ...
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bioRxiv - Neuroscience 2023Quote: ... Sections (50 µm thickness) were cut via freezing microtome and stained with anti-c-fos primary antibody (Cell Signaling Technology 2250T or Novus Biologicals NBP2-50037SS), and nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Neuroscience 2020Quote: ... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 800 - 1000 µg protein in cytoplasmic subcellular fractions of SH-SY5Y cells were incubated with either 10 µg anti-ATM antibody (Novus Biologicals, Littleton, Colorado, USA, NB100-220) or 10 µg of mouse IgG1 isotype control antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: Primary antibodies and the optimal dilutions and concentrations used are as follows: goat polyclonal anti-spike S2 (Novus Biologicals, AF10774-SP, 1:20, 10 µg/mL), mouse monoclonal anti-dsRNA (SCICONS ...
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bioRxiv - Immunology 2019Quote: ... Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (R&D AF771 goat anti-IL-1R, 1:50; Novus Biologicals NB600-408 rabbit anticollagen 1, 1:50). The next morning ...
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bioRxiv - Biophysics 2023Quote: ... The blot was blocked in 5% BSA in TBST and subsequently incubated with primary antibody (anti-mDia1, #20624-1-AP, Thermo Fischer Scientific, 1:1000, overnight at 4° C; GAPDH, #NB300-221, Novus Biologicals, 1:40.000, 1-2 hrs at RT). Secondary antibody staining was performed for 1 hour at RT in blocking solution (Donkey-Anti-Rabbit-HRP ...