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Citations for Novus Biologicals :
1401 - 1450 of 1453 citations for 2R 5S 5 4 amino 5 fluoro 2 oxopyrimidin 1 2H yl 1 3 oxathiolan 2 yl methyl butyrate WXC04778 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Primary antibodies against the following target proteins were then used to probe for NLRP3 (rabbit; 1:1000; NBP1-77080, Novus Biologicals), ASC (mouse ...
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bioRxiv - Physiology 2024Quote: ... and GluN2B subunits of NMDAR were detected using goat polyclonal primary antibodies anti-NMDAR1 (1:200, ref. NB100-41105; Novus Biologicals), mouse monoclonal anti-NMDAe1 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were simultaneously incubated with 100 μM A-769662 (or DMSO) and 1 mg/mL β1-integrin antibody clone K20 (Novus Biologicals) for the indicated amount of time (2-5 min ...
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bioRxiv - Immunology 2022Quote: ... primary MDMs from 3 different donors were pre-incubated with 20 μg/ml anti-CD169 antibody (HSn 7D2, Novus Biologicals) or IgG1k (P3.6.2.8.1 ...
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bioRxiv - Immunology 2022Quote: ... HMGB-1 was determined in the cell-free TA supernatants and in the supernatants of Calu-3 cultures using immunoassay following the manufacturer’s instructions (Novus Biologicals). Adenosine 5′-triphosphate (ATP ...
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bioRxiv - Developmental Biology 2021Quote: ... The frozen sections or fixed cells were treated with the following primary antibodies: anti-BrdU (rat, NB500-169, Novus Biologicals, 1:2000); anti-βIII-tubulin (mouse ...
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bioRxiv - Molecular Biology 2020Quote: ... then the PVDF membrane was incubated together with HRP-conjugated secondary antibody (HRP-linked anti-rabbit IgG antibody, 1:3000, cat. no. NB710-57836; Novus Biologicals, LLC) at room temperature for 1.5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were stained with primary chicken polyclonal antibody against the 160 kDa fragment of neurofilament (1:500) (Novus Biologicals; NB300-222) and the secondary antibody of AlexaFluor 488 goat anti-chicken IgY (1:400 ...
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bioRxiv - Cell Biology 2022Quote: ... These membranes were blocked in 6% nonfat milk for 1.5 h at room temperature and then probed with antibodies against survivin (cat. no. NB500-201, Novus Biologicals; 1:200), cyclin D1 (cat ...
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bioRxiv - Immunology 2021Quote: ... IHC on mouse bones was performed on deparaffinized and rehydrated sections with unconjugated primary antibodies against F4/80 (clone Cl:A3-1; Novus Biological, CO, USA), osteocalcin (polyclonal ...
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bioRxiv - Physiology 2024Quote: ... Aortic sections were incubated with goat anti-α-smooth muscle actin (1:500 dilution; Novus Biologicals, Centennial, CO, USA; Cat#: NB300-978), rabbit anti-smooth muscle myosin heavy chain 11 (1:500 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... and stable polyclonal cell lines were selected with 1 μg/ml puromycin and maintained in their respective media with tetracycline-free 10% FBS (Novus Biologicals #S10350) and 1 μg/ml puromycin ...
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bioRxiv - Immunology 2023Quote: ... γ-H2AX staining was performed in PBT buffer in a 1:10,000 dilution (gamma H2AX Ser139, Rabbit, Novus Biologicals, Englewood, CO, USA) and incubated overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... membrane transfer and immunoblotting were performed as previously described (50) using the following antibodies: anti-53BP1 (1:2000, Novus Biologicals #NBP2-54753SS), anti-ATM (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Blots were incubated overnight at 4°C with following primary antibodies: LC3B (Novus Biologicals, cat. no. NB600-1384), uroplakin Ia (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained overnight at 4°C using Lamp2 mouse monoclonal antibody (Novus Biologicals, Cat# NBP2-22217, RRID:AB_2722697)(1/200) ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 4×106 cells were used per IP with 3.65 µg of antibodies targeting HIF2α (Novus Biologicals, NB100122) or normal rabbit IgG (Cell Signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 time for 5 mins and 3 times for 2 hours in PBS supplemented with 0.3% Triton X-100 (PBS-T) and then incubated in primary antibody solution in PBS-T (goat anti-fluorescein (Novus Biologicals NB600-493 ...
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were incubated overnight at 4°C in blocking buffer with rabbit anti-human type VI collagen and rabbit anti-human type XII collagen primary antibodies (each 1:200, Novus Biologicals, Littleton, CO). Microcarriers were washed with PBS and incubated in goat anti-rabbit Fluorescein-conjugated secondary antibody (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... IHC was conducted using rabbit polyclonal antibodies raised against the SARS-CoV nucleocapsid protein (clone NB100-56576 [1:100], Novus Biologicals, Littleton, CO) and biotinylated anti-rabbit IgG ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry (IHC) was conducted on consecutive paraffin-embedded sections using the primary antibodies against HIF1α (rabbit polyclonal, Novus Biological, NB100-479, 1:500), SF3B1 (rabbit monoclonal ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Sections were then blocked with normal goat serum and incubated at room temperature with a primary antibody against CA-IX (1:500, Novus Biologicals, Cambridge, UK) or HIF1α (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154; anti-POLR1A, Novus Biologicals, NBP2-56122 ...
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bioRxiv - Cell Biology 2022Quote: ... Rat monoclonal anti-MNR were used (1/500; Chevrier et al, 2016) for Microtubule displacement assays while anti MNR (Novus Biological NBP1-90929) for expansion microscopy ...
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bioRxiv - Developmental Biology 2021Quote: ... and non-specific staining was blocked using 10% fetal bovine serum/neonatal goat serum for 1 hr prior 90 min incubation with either biotinylated anti-laminin antibody (Novus Biologicals, Colorado, USA) or unconjugated primary antibody against IBA1 (FUJIFILM Wako Chemicals ...
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bioRxiv - Immunology 2020Quote: ... a standard curve of absorbance value was obtained using 1-500 pg/ml concentration of recombinant ESAT-6 (NBP1-99045; Novus Biologicals, Centennial, CO).
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bioRxiv - Molecular Biology 2024Quote: ... Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 µL of smFISH probes (from 100 ng/µL smFISH probe stock) ...
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bioRxiv - Genomics 2024Quote: ... We performed flow cytometry (MACSquant VYB) to confirm that >90% of the PGP1 hiPSCs are positive for TRA-1-60 (Novus Biologicals NB100-730F488). hiPSCs were plated using StemFlex media (Thermo Scientific A3349401 ...
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bioRxiv - Immunology 2024Quote: ... Detection of bound bat IgGs was done by incubation for 1 hour at room temperature with mouse anti-bat immunoglobulin (L-chain specific, clone BT1-4F10, Novus Biologicals, CO, USA) diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech ...
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bioRxiv - Immunology 2024Quote: ... Detection of bound bat F(ab’)2 fragments was done by incubation for 1 hour at room temperature with mouse anti-bat immunoglobulin (L-chain specific, clone BT1-4F10, Novus Biologicals, CO, USA) diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331; anti-SRRM2, Thermo Fisher PA5-59559; anti-SC35, Novus Biologicals NB100-1774 ...
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bioRxiv - Cell Biology 2024Quote: ... then washed with PBS and incubated for 1 h with a secondary donkey-anti rabbit antibodies conjugated to DyLight 488nm (Novus Biologicals, Toronto, ON). Cells were then washed three times with PBS and mounted using DAKO fluorescence mounting media (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated overnight at 4°C with the following primary antibodies: anti-human CD63 (Novus Biologicals® H5C6), anti-eGFP (BIO-RAD 81/4745-1051) ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were incubated overnight at 4°C with the primary antibody anti-FII (Novus biologicals, Centennial, CO, USA) or Beta-actin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated overnight at 4°C with an anti-ACADV polyclonal antibody (Novus Biologicals, Centennial, CO, USA) and an anti-SDHA monoclonal antibody (Cell Signaling Technology ...
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bioRxiv - Genetics 2023Quote: ... Isolated nuclei were first stained for 40 minutes at 4°C with Cardiac Troponin T (Novus Biological NB120-10214AF488) at a 1:100 dilution in 0.01% BSA ...
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bioRxiv - Immunology 2024Quote: ... The resulting supernatant was quantified using cytokine specific ELISA kits (IL-1β Invitrogen #88-7013-22; IL-18 Thermo Fisher #BMS618-3; GzmB R&D #DY1865; Perforin Novus Biologicals # NBP3-00452 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed with TBS-T for 10 min before incubating for 1 h at room temperature with a secondary antibody conjugated to goat anti-mouse HRP (NB7539; Novus Biologicals, Centennial, CO, USA) with a titre of 1:10,000 in BLOTTO ...
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively). Cells were incubated with the primary antibody solution overnight at 4 °C then washed with PBSx3 ...
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bioRxiv - Cell Biology 2024Quote: ... 60 µm thick vibratome sections were then immunostained with antibodies against Choline Acetyl-Transferase (ChAT) (anti-ChAT, Novus Biologicals, goat, NBP1-30052, 1:10) and HA (anti-HA ...
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K; GDF-15: R&D Systems, MGD150; Adiponectin: Crystal Chem, 80569; FABP4: Novus biologicals, NBP2-82410 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1.5 h before they were incubated overnight at 4°C with antibodies to survivin (cat. no. NB500-201, Novus Biologicals), collagen (cat ...
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bioRxiv - Bioengineering 2024Quote: Mouse flat mounts were blocked and permeabilized overnight at 4°C in RBB before being incubated with rabbit anti-RbPMS (1.6 mg/mL; NBP2-20112, Novus Biologicals) diluted 1:400 in RBB overnight at 4°C ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617, Novus Biologicals, Centennial, CO, USA; 1:1,000 dilution); mouse anti-α-actinin antibody (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2020Quote: ... Blocking was for 30 minutes with PBST/1%BSA at 4 °C after which antibody staining was performed (anti-cyclin A2-Alexa Fluor 555, Abcam ab217731; anti-53BP1, Novus Biologicals NB100-94 self-conjugated to Alexa Fluor 647 ...
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bioRxiv - Cancer Biology 2021Quote: Liver metastatic tissue was sliced at 10 μm thickness with a microtome and stained overnight at 4 °C with an anti-Ki67 antibody (Novus Biologicals). The mean numbers of positive cells at 400⍰ magnification were compared between the two groups ...
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bioRxiv - Cancer Biology 2024Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with antibodies against IκBζ (Cell Signaling, Cat. 9244; Novus Biologicals, Cat ...
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bioRxiv - Neuroscience 2020Quote: ... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Primary antibodies and the optimal dilutions and concentrations used are as follows: goat polyclonal anti-spike S2 (Novus Biologicals, AF10774-SP, 1:20, 10 µg/mL), mouse monoclonal anti-dsRNA (SCICONS ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were extensively washed up to eight times in blocking solution and the following antibodies were applied in blocking buffer for immunolabeling: Chicken polyclonal MAP2 (Novus Biologicals NB300-213, diluted 1:1000), Rabbit polyclonal Munc13-1 (Synaptic Systems AB_126103 ...