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Citations for Novus Biologicals :
1351 - 1389 of 1389 citations for N1 N2 Dimesitylethane 1 2 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... membrane transfer and immunoblotting were performed as previously described (50) using the following antibodies: anti-53BP1 (1:2000, Novus Biologicals #NBP2-54753SS), anti-ATM (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated for 30 min at room temperature in blocking buffer (3% BSA in PBS) and then overnight at 4°C with anti Myo1b antibody (1:200, Novus Biologicals NBP1-87739) in blocking ...
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were incubated overnight at 4°C in blocking buffer with rabbit anti-human type VI collagen and rabbit anti-human type XII collagen primary antibodies (each 1:200, Novus Biologicals, Littleton, CO). Microcarriers were washed with PBS and incubated in goat anti-rabbit Fluorescein-conjugated secondary antibody (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... IHC was conducted using rabbit polyclonal antibodies raised against the SARS-CoV nucleocapsid protein (clone NB100-56576 [1:100], Novus Biologicals, Littleton, CO) and biotinylated anti-rabbit IgG ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry (IHC) was conducted on consecutive paraffin-embedded sections using the primary antibodies against HIF1α (rabbit polyclonal, Novus Biological, NB100-479, 1:500), SF3B1 (rabbit monoclonal ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Sections were then blocked with normal goat serum and incubated at room temperature with a primary antibody against CA-IX (1:500, Novus Biologicals, Cambridge, UK) or HIF1α (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154; anti-POLR1A, Novus Biologicals, NBP2-56122 ...
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bioRxiv - Cell Biology 2022Quote: ... Rat monoclonal anti-MNR were used (1/500; Chevrier et al, 2016) for Microtubule displacement assays while anti MNR (Novus Biological NBP1-90929) for expansion microscopy ...
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bioRxiv - Developmental Biology 2021Quote: ... and non-specific staining was blocked using 10% fetal bovine serum/neonatal goat serum for 1 hr prior 90 min incubation with either biotinylated anti-laminin antibody (Novus Biologicals, Colorado, USA) or unconjugated primary antibody against IBA1 (FUJIFILM Wako Chemicals ...
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bioRxiv - Immunology 2020Quote: ... a standard curve of absorbance value was obtained using 1-500 pg/ml concentration of recombinant ESAT-6 (NBP1-99045; Novus Biologicals, Centennial, CO).
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: anti-CD11b (1:2000; Novus Biologicals, Littleton, CO), anti-TNFα (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... primary antibody at a concentration of 1 in 200 in blocking buffer at 4°C overnight or a rabbit anti-53BP1 primary antibody (Novus Biologicals NB100-304) at 1 in 500 in blocking buffer at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 µL of smFISH probes (from 100 ng/µL smFISH probe stock) ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were incubated with primary antibodies rabbit polyclonal anti-caspase 3 and rat monoclonal anti-CD31/PECAM-1 antibody (Novus Biologicals, Centennial, CO) in 1% normal goat serum ...
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bioRxiv - Genomics 2024Quote: ... We performed flow cytometry (MACSquant VYB) to confirm that >90% of the PGP1 hiPSCs are positive for TRA-1-60 (Novus Biologicals NB100-730F488). hiPSCs were plated using StemFlex media (Thermo Scientific A3349401 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by overnight incubation (PMSNs) or 96 h (DRGs) at 4°C with the mouse monoclonal anti-CGRP (1:200, Novus Biologicals, Cat# NB036540), chicken polyclonal to Neurofilament heavy polypeptide NF200 (1:250 ...
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bioRxiv - Immunology 2024Quote: ... Detection of bound bat IgGs was done by incubation for 1 hour at room temperature with mouse anti-bat immunoglobulin (L-chain specific, clone BT1-4F10, Novus Biologicals, CO, USA) diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech ...
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bioRxiv - Immunology 2024Quote: ... Detection of bound bat F(ab’)2 fragments was done by incubation for 1 hour at room temperature with mouse anti-bat immunoglobulin (L-chain specific, clone BT1-4F10, Novus Biologicals, CO, USA) diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331; anti-SRRM2, Thermo Fisher PA5-59559; anti-SC35, Novus Biologicals NB100-1774 ...
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bioRxiv - Cell Biology 2024Quote: ... then washed with PBS and incubated for 1 h with a secondary donkey-anti rabbit antibodies conjugated to DyLight 488nm (Novus Biologicals, Toronto, ON). Cells were then washed three times with PBS and mounted using DAKO fluorescence mounting media (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed with TBS-T for 10 min before incubating for 1 h at room temperature with a secondary antibody conjugated to goat anti-mouse HRP (NB7539; Novus Biologicals, Centennial, CO, USA) with a titre of 1:10,000 in BLOTTO ...
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bioRxiv - Neuroscience 2021Quote: ... 3 x 5 minutes and then processed according to the technique of immunofluorescence labeling with overnight incubation at 4 °C with the polyclonal anti-Iba1 1:250 (# NB100-1028, Novus Biologicals, Littleton, Colorado, USA), monoclonal anti-GFAP 1:500 (# MAB3402X ...
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively). Cells were incubated with the primary antibody solution overnight at 4 °C then washed with PBSx3 ...
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bioRxiv - Cell Biology 2024Quote: ... 60 µm thick vibratome sections were then immunostained with antibodies against Choline Acetyl-Transferase (ChAT) (anti-ChAT, Novus Biologicals, goat, NBP1-30052, 1:10) and HA (anti-HA ...
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K; GDF-15: R&D Systems, MGD150; Adiponectin: Crystal Chem, 80569; FABP4: Novus biologicals, NBP2-82410 ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 minutes prior to being incubated o/n and at 4 °C with specific antibodies against TNF-α (MACS miltenyi biotec, #130-091-649), Vamp-3 (Abcam #Ab5789, 1:200 final dilution, the ER marker PDI (Novus Biologicals #NBP2-02082), the Golgi cis/trans marker GM130 (BD transduction laboratories #610822) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently incubated for 48 hours at 4°C with primary antibodies: rabbit-anti neurokinin B (1:1000, Novus Biologicals, catalog #NB300-201), guinea pig anti-cFos (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... and then treated with 4 N HCl for 20 min at 25°C before immunostaining with anti-BrdU (1:200; Novus Biologicals NB500-169, RRID:AB_10002608) primary antibodies and Alexa568-conjugated secondary antibodies (1:300) ...
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bioRxiv - Developmental Biology 2021Quote: ... permeabilized gonads were subsequently incubated with primary antibodies overnight at 4°C [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), rabbit anti-V5 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 5% powdered milk + PBS-T with primary antibodies [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), mouse anti-V5 (1:3000 ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617, Novus Biologicals, Centennial, CO, USA; 1:1,000 dilution); mouse anti-α-actinin antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10 μg of total proteins were loaded for each sample on the Invitrogen 4-12% Bis-Tris gel and the following antibodies were used for immunoblot detection: rabbit anti-Synj1 (Novus Biologicals, NBP1-87842, 1:1000), rabbit anti-GFAP (Abclonal ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for 1 hour and incubated overnight at 4°C with the NPC1 primary antibody (rabbit polyclonal, Novus Biologicals, UK, NB 400-148).
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were blocked with 5% non-fat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: heparanase (1:200 dilution; Novus Biologicals, Cat# NBP303846, RRID: AB_2927437) diluted in 5% non-fat milk ...
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bioRxiv - Cell Biology 2024Quote: ... for 15 min and blocked with 4% BSA in PBS for 1 h and overnight incubation of α-SMA antibody (Novus biologicals, Cat#NB300-978) diluted (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were incubated in 5% normal donkey serum in PBST overnight at 4°C: IBA1 (Novus Biologicals #NB100-1028 in goat, 1:1000); 6E10 (BioLegend #803001 in mouse ...
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bioRxiv - Neuroscience 2020Quote: ... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Primary antibodies and the optimal dilutions and concentrations used are as follows: goat polyclonal anti-spike S2 (Novus Biologicals, AF10774-SP, 1:20, 10 µg/mL), mouse monoclonal anti-dsRNA (SCICONS ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were extensively washed up to eight times in blocking solution and the following antibodies were applied in blocking buffer for immunolabeling: Chicken polyclonal MAP2 (Novus Biologicals NB300-213, diluted 1:1000), Rabbit polyclonal Munc13-1 (Synaptic Systems AB_126103 ...