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Citations for Novus Biologicals :
951 - 991 of 991 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... monoclonal antibody (CDC Arbovirus Reference Collection) or envelope fusion-loop specific 4G2 (3.33 mg/ml) (BEI Cat# NR-50327, Novus Biologicals Cat# NBP2-52709FR). Secondary antibody goat anti-Mouse HRP (200 ng/ml ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were identified for expression of mesenchymal markers such as CD29 and CD90 and the hematopoietic markers CD34 and CD45 (all antibodies obtained from Novus Biologicals company, US). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... Immunofluorescence images of DMSO and dTAG-13 treated WT Hela cells and TCOF1 degron cells stained using TCOF1 antibody (Novus Biologicals, NBP1-86909) and HA antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... A second set of ovaries was imaged using N-terminal eIF4ENIF1 rabbit antibody for comparison (2 μg/ml, NBP1-89389, Novus Biologicals, Littleton, CO). Slides were incubated in anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 µL of smFISH probes (from 100 ng/µL smFISH probe stock) ...
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bioRxiv - Bioengineering 2024Quote: Collected blood extracted from each animal was processed into plasma and levels of rhA1AT or pdA1AT were measured via sandwich ELISA using a matched antibody pair specific for human A1AT (Novus Biologicals NBP2-79565) and following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Proteins were eluted using Laemmli buffer and analyzed by western blot using Goat anti-Human IgG Fc fragment Secondary Antibody [HRP] (Novus biological NBP1-75006) or anti-BMP6 antibody (R&D systems ...
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bioRxiv - Molecular Biology 2023Quote: ... The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000, NB100-56576, Novus Biological, CO, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in a primary antibody (anti-ATF4, Cell Signaling Technologies D4B8, 1:500; anti-MAP2, Novus Biologicals NB200-213, 1:5000) diluted in 1% BSA ...
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bioRxiv - Genomics 2022Quote: ... Sections were then incubated with primary antibodies (anti-NeuN: 1:50, MAB377X, Millipore, Billerica, Massachusetts, United States; anti-RBPMS:1:1000, Novus Biologicals NBP2-20112) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... then overnight with primary antibodies (1:500 RBPMS, PhosphoSolutions #1832-RBPMS; 1:400 CHX10, Novus Biologicals #NBP1-84476; 1:50 AP2A, DSHB #3B5) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... After 1 h blocking with 3% BSA in tween-tris buffered solution (TTBS) the proteins were detected with the antibody anti-CXCR7/RDC-1 (1:500) (Novus Biologicals, Centennial, USA) labeled with IRDyes and was incubated for 90min at room temperature in 3% BSA in TTBS ...
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bioRxiv - Bioengineering 2024Quote: ... All transcripts were purified by silica-column chromatography followed by cellulose purification to remove dsRNA impurities.44 The presence of dsRNA impurities was determined by dot blot (500 ng/dot) using J2 dsRNA-specific antibody (Novus Biologicals, NBP3-11395). The length and integrity of mRNA were validated on an Agilent TapeStation system (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... The antibody against K11-linked ubiquitin chains (Cat. No. NBP3-05681, kindly provided by A. Buchberger,) was purchased from Novus Biologicals (Littleton, USA). The antibody against Sam50 was custom- generated against the full-length protein (Gramsch laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331; anti-SRRM2, Thermo Fisher PA5-59559; anti-SC35, Novus Biologicals NB100-1774 ...
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bioRxiv - Cell Biology 2024Quote: ... then washed with PBS and incubated for 1 h with a secondary donkey-anti rabbit antibodies conjugated to DyLight 488nm (Novus Biologicals, Toronto, ON). Cells were then washed three times with PBS and mounted using DAKO fluorescence mounting media (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed with TBS-T for 10 min before incubating for 1 h at room temperature with a secondary antibody conjugated to goat anti-mouse HRP (NB7539; Novus Biologicals, Centennial, CO, USA) with a titre of 1:10,000 in BLOTTO ...
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bioRxiv - Immunology 2020Quote: ... The EVs pellet suspended in RIPA was used to perform western blot to check the expression of ESAT-6 (anti-ESAT-6 antibody (#ab26246, AbcamInc, USA) and CD63 (#NBP2-42225SS, Novus Biologicals, Centennial, CO, USA).
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively). Cells were incubated with the primary antibody solution overnight at 4 °C then washed with PBSx3 ...
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bioRxiv - Biophysics 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-UBTF, Novus Biologicals, NBP1-82545; anti-POLR1A, Novus Biologicals, NBP2-56122; anti-Fibrillarin, EMD Millipore, MABE1154; anti-MPP10, Novus Biologicals, NBP1-84341 ...
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bioRxiv - Cell Biology 2024Quote: ... 60 µm thick vibratome sections were then immunostained with antibodies against Choline Acetyl-Transferase (ChAT) (anti-ChAT, Novus Biologicals, goat, NBP1-30052, 1:10) and HA (anti-HA ...
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bioRxiv - Immunology 2024Quote: ... and then stained with anti-Rb antibodies and their corresponding secondary IgG (#MA5-11387 Thermo Fisher Scientific, Waltham, MA, #NB100-56598 Novus Biologicals, Centennial, CO).
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bioRxiv - Molecular Biology 2024Quote: ... Several clones were isolated and examined for the expression of Tet-R protein by western hybridization using an antibody against Tet-R (Novus Biologicals, cat# NB600-234). The clone expressing the highest amount of the repressor was transfected with construct pcDNA4-TO-bio-myc K294A generated in an earlier study (Mukherjee et al ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then probed overnight with mouse anti-PNMT primary antibody (1:1000 dilution in 2% w/V BSA in TBS-T; cat. no. NBP2-00688, Novus Biologicals, Centennial, CO, USA), or rabbit glucocorticoid receptor beta (GR-β)-A antibody (1:1000 dilution in 5% w/v BSA in TBS-T ...
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bioRxiv - Molecular Biology 2024Quote: ... IdU/CldU fibers were stained using standard immunostaining with antibodies against IdU (anti-BrdU, clone B44, Beckton Dickinson #347580, 1:50) and CldU (anti-BrdU BU1/75, Novus Biological ab6326, 1:100) followed by sequential labelling with secondary antibodies (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... For experimental IHC and mIHC we stained the following antibodies for 1 hour at room temperature at the described concentrations: ADPH (1:1000, Novus Biologicals Cat: NB110-40878), CD45 (1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 5% powdered milk + PBS-T with primary antibodies [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), mouse anti-V5 (1:3000 ...
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bioRxiv - Neuroscience 2020Quote: ... before incubation with primary antibodies (anti-NLGN3 C-terminus, Synaptic Systems, 1:1000, RRID: AB_2619816.; anti-NLGN3 N-terminus, Novus Biologicals, 1:1000, RRID: AB_11027178) in blocking buffer with 0.01% sodium azide for 10 minutes at room temperature ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617, Novus Biologicals, Centennial, CO, USA; 1:1,000 dilution); mouse anti-α-actinin antibody (Santa Cruz Biotechnology ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked with 5% milk in 1xTBS-Tween (10mM Tris, 150 mM NaCL, 0.1% Tween20) for 60 minutes then blotted with primary antibodies (Set8: Novus Biologicals 44710002; β-tubulin: Abcam ab6046) in 5% milk in 1xTBS-Tween overnight ...
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bioRxiv - Physiology 2023Quote: ... Incubation with primary antibody was followed by 3 washes and membranes were incubated 2 hours with goat anti-Human IgG (Novus biological, NBP1-75006, 1/10000), goat anti-rabbit IgG (Cell signaling ...
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bioRxiv - Neuroscience 2023Quote: ... Sections (50 µm thickness) were cut via freezing microtome and stained with anti-c-fos primary antibody (Cell Signaling Technology 2250T or Novus Biologicals NBP2-50037SS), and nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were probed with the following antibodies: SLC7A11 (Cell Signalling Technologies, Cat. 12691, 1:25; Abcam, Cat. ab37185, 1:2000; Novus Biologicals, Cat. NB300-318, 1:2000), biotinylated anti-rabbit secondary antibody (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... cells are incubated with the primary antibody anti-myosin heavy chain (MF20, mouse monoclonal, DHSB, diluted 1:2) or anti-ki67 (rabbit polyclonal, Novus Biologicals #NB110-89717, diluted 1:200) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154; anti-POLR1A, Novus Biologicals, NBP2-56122; anti-UBTF, Novus Biologicals, NBP1-82545) in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... a consecutive heart section was treated similarly except that the primary MC1-R antibody was replaced by purified normal rabbit IgG (Novus Biologicals, Littleton, CO, USA, # NB810-56910). Sections were counterstained with hematoxylin (CarlRoth) ...
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bioRxiv - Cell Biology 2024Quote: ... 800 - 1000 µg protein in cytoplasmic subcellular fractions of SH-SY5Y cells were incubated with either 10 µg anti-ATM antibody (Novus Biologicals, Littleton, Colorado, USA, NB100-220) or 10 µg of mouse IgG1 isotype control antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: Primary antibodies and the optimal dilutions and concentrations used are as follows: goat polyclonal anti-spike S2 (Novus Biologicals, AF10774-SP, 1:20, 10 µg/mL), mouse monoclonal anti-dsRNA (SCICONS ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were extensively washed up to eight times in blocking solution and the following antibodies were applied in blocking buffer for immunolabeling: Chicken polyclonal MAP2 (Novus Biologicals NB300-213, diluted 1:1000), Rabbit polyclonal Munc13-1 (Synaptic Systems AB_126103 ...
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bioRxiv - Physiology 2024Quote: ... chromatin extracts were prepared from FXR floxed and FXR-LKO mouse livers after treatment of the mice with GW4064 which was followed by preclearing and immunoprecipitation using control IgG or FXR antibody (Novus Biologicals, NBP2-16550; Santa Cruz, sc-25309), RXRα antibody (Proteintech ...