Labshake search
Citations for Novus Biologicals :
901 - 950 of 967 citations for Gamma secretase subunit APH 1B APH1B Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Then the cells were labeled with antibodies against cleaved caspase-3 (1:20, CST #9602S) or Myogenin (1:20, Novus Biologicals #NBP2-33056). The following isotype controls were used ...
-
bioRxiv - Genetics 2020Quote: ... Membranes were blocked with 5% w/v nonfat dried milk and incubated with the following primary antibodies: anti-CHD8 (Novus Biologicals NB-10060417) (1:1000) ...
-
bioRxiv - Bioengineering 2021Quote: ... Microcarriers were incubated overnight at 4°C in blocking buffer with rabbit anti-human type VI collagen and rabbit anti-human type XII collagen primary antibodies (each 1:200, Novus Biologicals, Littleton, CO). Microcarriers were washed with PBS and incubated in goat anti-rabbit Fluorescein-conjugated secondary antibody (1:500 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The SARS-CoV-2 and SARS-CoV viral lysates were immuno-detected with a SARS spike protein antibody (NB-100-56578, Novus Biological, Littleton, CO), followed by an anti-rabbit-IgG HRP linked whole antibody (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... IHC was conducted using rabbit polyclonal antibodies raised against the SARS-CoV nucleocapsid protein (clone NB100-56576 [1:100], Novus Biologicals, Littleton, CO) and biotinylated anti-rabbit IgG ...
-
bioRxiv - Neuroscience 2020Quote: ... Free-floating 30 μm sections were blocked with 4% goat or horse serum/PBS plus 0.2% Triton X-100 and incubated with an antibody against TH (Novus Biologicals, Littleton, CO, USA) followed by incubation with biotin-conjugated anti-rabbit antibody or biotin-conjugated anti-mouse antibody (Vectastain Elite ABC Kit ...
-
bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry (IHC) was conducted on consecutive paraffin-embedded sections using the primary antibodies against HIF1α (rabbit polyclonal, Novus Biological, NB100-479, 1:500), SF3B1 (rabbit monoclonal ...
-
Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Sections were then blocked with normal goat serum and incubated at room temperature with a primary antibody against CA-IX (1:500, Novus Biologicals, Cambridge, UK) or HIF1α (1:500 ...
-
bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154; anti-POLR1A, Novus Biologicals, NBP2-56122 ...
-
bioRxiv - Microbiology 2022Quote: ... Then cells were washed twice with PBS and incubated with a specific antibody (α-STX 17 (Protein-Tech) or α-LC3B(Novus Biologicals) in a blocking buffer containing 2 % BSA and 0.01% saponin for 3 hours at room temperature (RT ...
-
bioRxiv - Developmental Biology 2021Quote: ... and non-specific staining was blocked using 10% fetal bovine serum/neonatal goat serum for 1 hr prior 90 min incubation with either biotinylated anti-laminin antibody (Novus Biologicals, Colorado, USA) or unconjugated primary antibody against IBA1 (FUJIFILM Wako Chemicals ...
-
bioRxiv - Microbiology 2021Quote: ... monoclonal antibody (CDC Arbovirus Reference Collection) or envelope fusion-loop specific 4G2 (3.33 mg/ml) (BEI Cat# NR-50327, Novus Biologicals Cat# NBP2-52709FR). Secondary antibody goat anti-Mouse HRP (200 ng/ml ...
-
bioRxiv - Bioengineering 2020Quote: ... The cells were identified for expression of mesenchymal markers such as CD29 and CD90 and the hematopoietic markers CD34 and CD45 (all antibodies obtained from Novus Biologicals company, US). Briefly ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: anti-CD11b (1:2000; Novus Biologicals, Littleton, CO), anti-TNFα (1:2000 ...
-
bioRxiv - Cell Biology 2021Quote: ... primary antibody at a concentration of 1 in 200 in blocking buffer at 4°C overnight or a rabbit anti-53BP1 primary antibody (Novus Biologicals NB100-304) at 1 in 500 in blocking buffer at 4°C overnight ...
-
bioRxiv - Physiology 2023Quote: ... Proteins were eluted using Laemmli buffer and analyzed by western blot using Goat anti-Human IgG Fc fragment Secondary Antibody [HRP] (Novus biological NBP1-75006) or anti-BMP6 antibody (R&D systems ...
-
bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in a primary antibody (anti-ATF4, Cell Signaling Technologies D4B8, 1:500; anti-MAP2, Novus Biologicals NB200-213, 1:5000) diluted in 1% BSA ...
-
bioRxiv - Molecular Biology 2023Quote: ... The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000, NB100-56576, Novus Biological, CO, USA) overnight at 4°C ...
-
bioRxiv - Bioengineering 2024Quote: Collected blood extracted from each animal was processed into plasma and levels of rhA1AT or pdA1AT were measured via sandwich ELISA using a matched antibody pair specific for human A1AT (Novus Biologicals NBP2-79565) and following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... A second set of ovaries was imaged using N-terminal eIF4ENIF1 rabbit antibody for comparison (2 μg/ml, NBP1-89389, Novus Biologicals, Littleton, CO). Slides were incubated in anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 594 (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 µL of smFISH probes (from 100 ng/µL smFISH probe stock) ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.2% Triton X-100 and 1% bovine serum albumin for 1 hour and then stained overnight at 4°C with primary antibodies sheep anti- TH (1:1000, Novus biologicals, NB300-109), rabbit anti-Vamp2 (1:1000 ...
-
bioRxiv - Biophysics 2022Quote: ... Immunofluorescence images of DMSO and dTAG-13 treated WT Hela cells and TCOF1 degron cells stained using TCOF1 antibody (Novus Biologicals, NBP1-86909) and HA antibody (Invitrogen ...
-
bioRxiv - Genomics 2022Quote: ... Sections were then incubated with primary antibodies (anti-NeuN: 1:50, MAB377X, Millipore, Billerica, Massachusetts, United States; anti-RBPMS:1:1000, Novus Biologicals NBP2-20112) diluted in blocking buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... then overnight with primary antibodies (1:500 RBPMS, PhosphoSolutions #1832-RBPMS; 1:400 CHX10, Novus Biologicals #NBP1-84476; 1:50 AP2A, DSHB #3B5) at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... After 1 h blocking with 3% BSA in tween-tris buffered solution (TTBS) the proteins were detected with the antibody anti-CXCR7/RDC-1 (1:500) (Novus Biologicals, Centennial, USA) labeled with IRDyes and was incubated for 90min at room temperature in 3% BSA in TTBS ...
-
bioRxiv - Bioengineering 2024Quote: ... All transcripts were purified by silica-column chromatography followed by cellulose purification to remove dsRNA impurities.44 The presence of dsRNA impurities was determined by dot blot (500 ng/dot) using J2 dsRNA-specific antibody (Novus Biologicals, NBP3-11395). The length and integrity of mRNA were validated on an Agilent TapeStation system (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... 250ug of lysate was precleared with 20 μl of packed Protein A Sepharose CL-4B resin (Cytivia, 17078001) for 2 hours at 4 °C and then incubated overnight with 3ug of anti-NPM1 antibody (Novus Biologicals, NB600-1030) at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... The antibody against K11-linked ubiquitin chains (Cat. No. NBP3-05681, kindly provided by A. Buchberger,) was purchased from Novus Biologicals (Littleton, USA). The antibody against Sam50 was custom- generated against the full-length protein (Gramsch laboratories ...
-
bioRxiv - Cell Biology 2024Quote: ... then washed with PBS and incubated for 1 h with a secondary donkey-anti rabbit antibodies conjugated to DyLight 488nm (Novus Biologicals, Toronto, ON). Cells were then washed three times with PBS and mounted using DAKO fluorescence mounting media (Agilent ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331; anti-SRRM2, Thermo Fisher PA5-59559; anti-SC35, Novus Biologicals NB100-1774 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed with TBS-T for 10 min before incubating for 1 h at room temperature with a secondary antibody conjugated to goat anti-mouse HRP (NB7539; Novus Biologicals, Centennial, CO, USA) with a titre of 1:10,000 in BLOTTO ...
-
bioRxiv - Immunology 2020Quote: ... The EVs pellet suspended in RIPA was used to perform western blot to check the expression of ESAT-6 (anti-ESAT-6 antibody (#ab26246, AbcamInc, USA) and CD63 (#NBP2-42225SS, Novus Biologicals, Centennial, CO, USA).
-
Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively). Cells were incubated with the primary antibody solution overnight at 4 °C then washed with PBSx3 ...
-
bioRxiv - Cell Biology 2024Quote: ... 60 µm thick vibratome sections were then immunostained with antibodies against Choline Acetyl-Transferase (ChAT) (anti-ChAT, Novus Biologicals, goat, NBP1-30052, 1:10) and HA (anti-HA ...
-
bioRxiv - Biophysics 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-UBTF, Novus Biologicals, NBP1-82545; anti-POLR1A, Novus Biologicals, NBP2-56122; anti-Fibrillarin, EMD Millipore, MABE1154; anti-MPP10, Novus Biologicals, NBP1-84341 ...
-
bioRxiv - Molecular Biology 2024Quote: ... IdU/CldU fibers were stained using standard immunostaining with antibodies against IdU (anti-BrdU, clone B44, Beckton Dickinson #347580, 1:50) and CldU (anti-BrdU BU1/75, Novus Biological ab6326, 1:100) followed by sequential labelling with secondary antibodies (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... The membrane was then probed overnight with mouse anti-PNMT primary antibody (1:1000 dilution in 2% w/V BSA in TBS-T; cat. no. NBP2-00688, Novus Biologicals, Centennial, CO, USA), or rabbit glucocorticoid receptor beta (GR-β)-A antibody (1:1000 dilution in 5% w/v BSA in TBS-T ...
-
bioRxiv - Immunology 2024Quote: ... and then stained with anti-Rb antibodies and their corresponding secondary IgG (#MA5-11387 Thermo Fisher Scientific, Waltham, MA, #NB100-56598 Novus Biologicals, Centennial, CO).
-
bioRxiv - Molecular Biology 2024Quote: ... Several clones were isolated and examined for the expression of Tet-R protein by western hybridization using an antibody against Tet-R (Novus Biologicals, cat# NB600-234). The clone expressing the highest amount of the repressor was transfected with construct pcDNA4-TO-bio-myc K294A generated in an earlier study (Mukherjee et al ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently incubated for 48 hours at 4°C with primary antibodies: rabbit-anti neurokinin B (1:1000, Novus Biologicals, catalog #NB300-201), guinea pig anti-cFos (1:1000 ...
-
bioRxiv - Cancer Biology 2024Quote: ... For experimental IHC and mIHC we stained the following antibodies for 1 hour at room temperature at the described concentrations: ADPH (1:1000, Novus Biologicals Cat: NB110-40878), CD45 (1:50 ...
-
bioRxiv - Developmental Biology 2021Quote: ... permeabilized gonads were subsequently incubated with primary antibodies overnight at 4°C [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), rabbit anti-V5 (1:1000 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 5% powdered milk + PBS-T with primary antibodies [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), mouse anti-V5 (1:3000 ...
-
bioRxiv - Neuroscience 2020Quote: ... before incubation with primary antibodies (anti-NLGN3 C-terminus, Synaptic Systems, 1:1000, RRID: AB_2619816.; anti-NLGN3 N-terminus, Novus Biologicals, 1:1000, RRID: AB_11027178) in blocking buffer with 0.01% sodium azide for 10 minutes at room temperature ...
-
Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617, Novus Biologicals, Centennial, CO, USA; 1:1,000 dilution); mouse anti-α-actinin antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Cell Biology 2021Quote: ... 5-10 μg of total proteins were loaded for each sample on the Invitrogen 4-12% Bis-Tris gel and the following antibodies were used for immunoblot detection: rabbit anti-Synj1 (Novus Biologicals, NBP1-87842, 1:1000), rabbit anti-GFAP (Abclonal ...
-
bioRxiv - Genetics 2022Quote: ... Membranes were blocked with 5% milk in 1xTBS-Tween (10mM Tris, 150 mM NaCL, 0.1% Tween20) for 60 minutes then blotted with primary antibodies (Set8: Novus Biologicals 44710002; β-tubulin: Abcam ab6046) in 5% milk in 1xTBS-Tween overnight ...
-
bioRxiv - Cell Biology 2021Quote: ... for 1 hour at room temperature and then incubated overnight at 4°C in a humid chamber with primary antibodies (SARS-CoV-2-N, GeneTex GTX635679, at 1:100; CD163,Novus Biologicals NBP2-48846, 1:250) diluted in 1% BSA/0.1% Triton-X PBS (PBST) ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for 1 hour and incubated overnight at 4°C with the NPC1 primary antibody (rabbit polyclonal, Novus Biologicals, UK, NB 400-148).