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Citations for Novus Biologicals :
901 - 950 of 954 citations for DEFA1 DEFA3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154; anti-POLR1A, Novus Biologicals, NBP2-56122 ...
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed twice with PBS and incubated with a specific antibody (α-STX 17 (Protein-Tech) or α-LC3B(Novus Biologicals) in a blocking buffer containing 2 % BSA and 0.01% saponin for 3 hours at room temperature (RT ...
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bioRxiv - Developmental Biology 2021Quote: ... and non-specific staining was blocked using 10% fetal bovine serum/neonatal goat serum for 1 hr prior 90 min incubation with either biotinylated anti-laminin antibody (Novus Biologicals, Colorado, USA) or unconjugated primary antibody against IBA1 (FUJIFILM Wako Chemicals ...
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bioRxiv - Microbiology 2021Quote: ... monoclonal antibody (CDC Arbovirus Reference Collection) or envelope fusion-loop specific 4G2 (3.33 mg/ml) (BEI Cat# NR-50327, Novus Biologicals Cat# NBP2-52709FR). Secondary antibody goat anti-Mouse HRP (200 ng/ml ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were identified for expression of mesenchymal markers such as CD29 and CD90 and the hematopoietic markers CD34 and CD45 (all antibodies obtained from Novus Biologicals company, US). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: anti-CD11b (1:2000; Novus Biologicals, Littleton, CO), anti-TNFα (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... primary antibody at a concentration of 1 in 200 in blocking buffer at 4°C overnight or a rabbit anti-53BP1 primary antibody (Novus Biologicals NB100-304) at 1 in 500 in blocking buffer at 4°C overnight ...
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bioRxiv - Biophysics 2022Quote: ... Immunofluorescence images of DMSO and dTAG-13 treated WT Hela cells and TCOF1 degron cells stained using TCOF1 antibody (Novus Biologicals, NBP1-86909) and HA antibody (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: Collected blood extracted from each animal was processed into plasma and levels of rhA1AT or pdA1AT were measured via sandwich ELISA using a matched antibody pair specific for human A1AT (Novus Biologicals NBP2-79565) and following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... A second set of ovaries was imaged using N-terminal eIF4ENIF1 rabbit antibody for comparison (2 μg/ml, NBP1-89389, Novus Biologicals, Littleton, CO). Slides were incubated in anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and blocking (1 h; 1% BSA, 5% horse serum) sections were incubated with primary antibodies for Megalin/LRP2 (1:200, Novus Biologicals NB110-96417), Calbindin D-28K (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 µL of smFISH probes (from 100 ng/µL smFISH probe stock) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Triton X-100 and 1% bovine serum albumin for 1 hour and then stained overnight at 4°C with primary antibodies sheep anti- TH (1:1000, Novus biologicals, NB300-109), rabbit anti-Vamp2 (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... Proteins were eluted using Laemmli buffer and analyzed by western blot using Goat anti-Human IgG Fc fragment Secondary Antibody [HRP] (Novus biological NBP1-75006) or anti-BMP6 antibody (R&D systems ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in a primary antibody (anti-ATF4, Cell Signaling Technologies D4B8, 1:500; anti-MAP2, Novus Biologicals NB200-213, 1:5000) diluted in 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000, NB100-56576, Novus Biological, CO, USA) overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Sections were then incubated with primary antibodies (anti-NeuN: 1:50, MAB377X, Millipore, Billerica, Massachusetts, United States; anti-RBPMS:1:1000, Novus Biologicals NBP2-20112) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... then overnight with primary antibodies (1:500 RBPMS, PhosphoSolutions #1832-RBPMS; 1:400 CHX10, Novus Biologicals #NBP1-84476; 1:50 AP2A, DSHB #3B5) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were permeabilized using PBS containing 0.1% saponin for 30 min and blocked using PBS with 1% BSA and 1% goat serum for 1 h before labeling with 1:100 dilution of rabbit polyclonal anti-TGN-46 antibody (Novus biologicals, NBP1-49643). After primary antibody labeling ...
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bioRxiv - Cell Biology 2024Quote: ... After 1 h blocking with 3% BSA in tween-tris buffered solution (TTBS) the proteins were detected with the antibody anti-CXCR7/RDC-1 (1:500) (Novus Biologicals, Centennial, USA) labeled with IRDyes and was incubated for 90min at room temperature in 3% BSA in TTBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 250ug of lysate was precleared with 20 μl of packed Protein A Sepharose CL-4B resin (Cytivia, 17078001) for 2 hours at 4 °C and then incubated overnight with 3ug of anti-NPM1 antibody (Novus Biologicals, NB600-1030) at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed with TBS-T for 10 min before incubating for 1 h at room temperature with a secondary antibody conjugated to goat anti-mouse HRP (NB7539; Novus Biologicals, Centennial, CO, USA) with a titre of 1:10,000 in BLOTTO ...
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bioRxiv - Immunology 2020Quote: ... The EVs pellet suspended in RIPA was used to perform western blot to check the expression of ESAT-6 (anti-ESAT-6 antibody (#ab26246, AbcamInc, USA) and CD63 (#NBP2-42225SS, Novus Biologicals, Centennial, CO, USA).
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively). Cells were incubated with the primary antibody solution overnight at 4 °C then washed with PBSx3 ...
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bioRxiv - Biophysics 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-UBTF, Novus Biologicals, NBP1-82545; anti-POLR1A, Novus Biologicals, NBP2-56122; anti-Fibrillarin, EMD Millipore, MABE1154; anti-MPP10, Novus Biologicals, NBP1-84341 ...
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bioRxiv - Cell Biology 2024Quote: ... 60 µm thick vibratome sections were then immunostained with antibodies against Choline Acetyl-Transferase (ChAT) (anti-ChAT, Novus Biologicals, goat, NBP1-30052, 1:10) and HA (anti-HA ...
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bioRxiv - Immunology 2024Quote: ... and then stained with anti-Rb antibodies and their corresponding secondary IgG (#MA5-11387 Thermo Fisher Scientific, Waltham, MA, #NB100-56598 Novus Biologicals, Centennial, CO).
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bioRxiv - Molecular Biology 2024Quote: ... Several clones were isolated and examined for the expression of Tet-R protein by western hybridization using an antibody against Tet-R (Novus Biologicals, cat# NB600-234). The clone expressing the highest amount of the repressor was transfected with construct pcDNA4-TO-bio-myc K294A generated in an earlier study (Mukherjee et al ...
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bioRxiv - Genetics 2019Quote: ... The membrane was then incubated with the NPC1 primary antibody (Abcam: ab106534 in main body figures or Novus Biologicals H00004864-M02 in Supplemental Fig. S4) at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... permeabilized gonads were subsequently incubated with primary antibodies overnight at 4°C [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), rabbit anti-V5 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 5% powdered milk + PBS-T with primary antibodies [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), mouse anti-V5 (1:3000 ...
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bioRxiv - Neuroscience 2020Quote: ... before incubation with primary antibodies (anti-NLGN3 C-terminus, Synaptic Systems, 1:1000, RRID: AB_2619816.; anti-NLGN3 N-terminus, Novus Biologicals, 1:1000, RRID: AB_11027178) in blocking buffer with 0.01% sodium azide for 10 minutes at room temperature ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617, Novus Biologicals, Centennial, CO, USA; 1:1,000 dilution); mouse anti-α-actinin antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10 μg of total proteins were loaded for each sample on the Invitrogen 4-12% Bis-Tris gel and the following antibodies were used for immunoblot detection: rabbit anti-Synj1 (Novus Biologicals, NBP1-87842, 1:1000), rabbit anti-GFAP (Abclonal ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked with 5% milk in 1xTBS-Tween (10mM Tris, 150 mM NaCL, 0.1% Tween20) for 60 minutes then blotted with primary antibodies (Set8: Novus Biologicals 44710002; β-tubulin: Abcam ab6046) in 5% milk in 1xTBS-Tween overnight ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at room temperature and then incubated overnight at 4°C in a humid chamber with primary antibodies (SARS-CoV-2-N, GeneTex GTX635679, at 1:100; CD163,Novus Biologicals NBP2-48846, 1:250) diluted in 1% BSA/0.1% Triton-X PBS (PBST) ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was incubated overnight at 4°C with primary antibody directed to ionized calcium-binding adaptor molecule 1 (IBA1) (Novus Biologicals NB100-1028, 1:500) diluted in CytoQ containing 0.05% Tween-20 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for 1 hour and incubated overnight at 4°C with the NPC1 primary antibody (rabbit polyclonal, Novus Biologicals, UK, NB 400-148).
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bioRxiv - Physiology 2023Quote: ... Incubation with primary antibody was followed by 3 washes and membranes were incubated 2 hours with goat anti-Human IgG (Novus biological, NBP1-75006, 1/10000), goat anti-rabbit IgG (Cell signaling ...
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bioRxiv - Neuroscience 2023Quote: ... Sections (50 µm thickness) were cut via freezing microtome and stained with anti-c-fos primary antibody (Cell Signaling Technology 2250T or Novus Biologicals NBP2-50037SS), and nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were blocked with nonfat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: sulfatase 1 (1:200 dilution; Novus Biologicals, Cat# NBP231584, RRID: AB_2916043) and sulfatase 2 (1:500 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were blocked with 5% non-fat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: heparanase (1:200 dilution; Novus Biologicals, Cat# NBP303846, RRID: AB_2927437) diluted in 5% non-fat milk ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were probed with the following antibodies: SLC7A11 (Cell Signalling Technologies, Cat. 12691, 1:25; Abcam, Cat. ab37185, 1:2000; Novus Biologicals, Cat. NB300-318, 1:2000), biotinylated anti-rabbit secondary antibody (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... cells are incubated with the primary antibody anti-myosin heavy chain (MF20, mouse monoclonal, DHSB, diluted 1:2) or anti-ki67 (rabbit polyclonal, Novus Biologicals #NB110-89717, diluted 1:200) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154; anti-POLR1A, Novus Biologicals, NBP2-56122; anti-UBTF, Novus Biologicals, NBP1-82545) in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were incubated in 5% normal donkey serum in PBST overnight at 4°C: IBA1 (Novus Biologicals #NB100-1028 in goat, 1:1000); 6E10 (BioLegend #803001 in mouse ...
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bioRxiv - Developmental Biology 2019Quote: ... then incubated overnight at 4°C in combined primary antibodies (chicken anti-green fluorescent protein [GFP]; Aves Labs, Tigard, OR; 1:500 and goat anti-c-Myc; Novus Biologicals, Littleton, CO; 1:200) diluted in TBS-BSA-Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... a consecutive heart section was treated similarly except that the primary MC1-R antibody was replaced by purified normal rabbit IgG (Novus Biologicals, Littleton, CO, USA, # NB810-56910). Sections were counterstained with hematoxylin (CarlRoth) ...
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bioRxiv - Cell Biology 2024Quote: ... 800 - 1000 µg protein in cytoplasmic subcellular fractions of SH-SY5Y cells were incubated with either 10 µg anti-ATM antibody (Novus Biologicals, Littleton, Colorado, USA, NB100-220) or 10 µg of mouse IgG1 isotype control antibody (Cell Signalling Technology ...