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Citations for Mirus Bio :
101 - 150 of 236 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were transfected using the Mirus TransIT®-LT1 Transfection Reagent (Cat# MIR-2304, Mirus Bio LLC, Madison, WI, USA) with the pSpCas9(BB)-2A-GFP plasmid (Cat# 48138 ...
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bioRxiv - Neuroscience 2023Quote: ... and 20.5 µg pcDNA3.1 VSV-G in 4.9 ml DMEM with 345 µl TransIT-Lenti Transfection Reagent (Mirus Bio, MIR 6600) added to 4.8 ml DMEM ...
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bioRxiv - Immunology 2023Quote: Retrovirus was generated by transfecting the Plat-E packaging cell line with 2.5 μg DNA and 7.5 μl TransIT-X2 Reagent (Mirus, MIR 600). B cells were isolated and cultured in complate 1640 RPMI medium before transduction ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 25,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx using TransIT-X2 (MIR 6003, Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... plus 10 µg of the vector of interest using Mirus TransIT-LT1 transfection reagent (Mirus Bio LLC, cat # MIR 2305). Medium was refreshed 16 h post-transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... we transfected S2 cells with pAC5.1-mcd8-CIBN-NTEV-NICD-GFP and pAC5.1-Cry2-mCherry-CTEV using the TransIT-Insect Reagent (Mirus, MIR #6104) according to manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293T-GFP cells were transiently transfected with wild-type and mutant spike plasmids using TransIT-X2 transfection reagent (Mirus #MIR 6000) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced by transfecting HEK293T with standard packaging vectors using the TransIT-LT1 Transfection Reagent (MIR 2306; Mirus Bio LLC). Viral supernatant was collected at 48h and 72 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... px459 CRISPR/Cas9 plasmids with or without gRNA sequence were transfected into HEK293T using TransIT-LT1 transfection reagent (Mirus, MIR 2306). Puromycin (1 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected the following day with pcDNA3.1/Paxillin-mApple or mEmerald-Lifeact at 20% confluency using TransIT-LT1 (Mirus MIR 2304) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: CHO-GFP-CCR5 cell lines were generated by transfecting CHO-K1 with a pCDNA3.1 Hygro-GFP-CCR5 construct using the TransIT-X2 transfection reagent from Mirus (MIR 6003). CCR5 expression was maintained by culturing cells in the presence of 400 µg/ml hygromycin and GFP-CCR5 expressing cells were isolated for single-cell cloning through the process of serial dilution cloning ...
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bioRxiv - Immunology 2020Quote: ... The cells were transfected with the sgRNA transfer plasmids (MSCV-U6-sgRNA-IRES-Thy1.1) using TransIT-293 transfection reagent per the manufacturer’s protocol (Mirus, Cat# MIR 2700). The following morning ...
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bioRxiv - Cell Biology 2021Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio, MIR 2705). Following transfection ...
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bioRxiv - Cell Biology 2020Quote: ... GFP-tagged E-cadherin or with constructs encoding E-cadherin and TNFα RUSH system using TransIT-LT1 Reagent (Mirus, MIR 2305) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... a gag-pol packaging construct (0.65 μg/well) and the relevant vector construct (1 μg/well) were transfected using the TransIT®-LT1 Transfection Reagent (MIR 2306, Mirus). Two days after transfection the soups containing the viruses were collected and filtered.
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bioRxiv - Cancer Biology 2022Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... Neuro2a cells were seeded in 24-well plates at 80,000 cells per well and on the next day were transfected with TransIT-LT1 Transfection Reagent (Mirus, MIR 2300), using 150 ng luciferase reporter ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Baculoviruses were produced by transfecting Sf9 cells with recombinant bacmids using TransIT®-Insect Transfection Reagent (Mirus, cat no. MIR 6100), grown for 7 days at 28ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... and rev genes and pMD2.G encoding the VSV-G envelope protein using the TransIT-Lenti transfection reagent (Mirus, MIR 6650). The virus was harvested after 48 hours and filtered through a 0.45 μm filter ...
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bioRxiv - Cancer Biology 2023Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... and maintained in the media described above supplemented with either 1.5ug/mL (HEK-293T and HeLa) or 10µg/mL puromycin (MDCK) (Mirus Bio, MIR 5940). A clonal MDCK-ALPL line with high transcytosis activity (MDCK-ALPL clone 21 ...
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bioRxiv - Cell Biology 2019Quote: ... plasmid vectors coding the glycoproteins were transfected into BSR-T7 cells using the TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR 2300). At 16 h post-transfection ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T-EGFP cells (donor cell) were transiently transfected with wild-type and mutant spike plasmids using TransIT-X2 transfection reagent (Mirus #MIR 6000). HEK293T-ACE2 and HEK293T (as a negative control ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 1 µg of DDX60 wt or mutant constructs using TransIT®-LT1 Transfection Reagent (Mirus Bio MIR 2300) at a 1:3 (µg to µl ...
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bioRxiv - Cell Biology 2022Quote: siRNA was delivered to differentiated adipocytes (6-7 days post-differentiation) using the TransIT-X2® dynamic delivery system (MIR 6004; Mirus Bio). Opti-MEM I (31985062 ...
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bioRxiv - Genetics 2021Quote: Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral vectors were incubated with 3rd generation viral packaging vectors (pCMV-VSV-G, pMDLg/pRRE, pRSV-Rev) and LT-1 (Mirus, MIR-2305) in OPTI-MEM media for 30 minutes as described by the provider’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-LT1 Transfection Reagent (MIR 2306; Mirus Bio LLC). Viral supernatant was collected 48–72 h after transfection ...
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bioRxiv - Microbiology 2024Quote: ... pVSV-G and either pLRGatewayIRESeFYP-Vpr/Vpx or pLRGatewayIRESmApple-Vpr/Vpx at a ratio 1:1:1 with TransIT-lenti (Mirus, MIR 6603). To generate HIV vectors to make stable cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed after 1 h and cells were transfected with a mixture of plasmids using TransIT-LT1 (Mirus Bio # MIR 2300), including pVSV-eGFP-deltaG_SARS-CoV-2 Spike (5 µg) ...
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bioRxiv - Genomics 2022Quote: Transection was performed in a 10-cm dish at 70%-80% confluence with 30 ul of TransIT 2020 transfection reagent (Mirus Bio, MIR 5400A) and 10μg of total DNA (1µg of pMT-ROWx2FLAG and 9 µg of bluescript plasmids) ...
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bioRxiv - Systems Biology 2020Quote: ... followed by incubation with 10fold diluted Label IT Amine Modifying Reagent in 1× Labeling Buffer A from Label IT Nucleic Acid Modifying Reagent (Mirus Bio MIR 3900) at room temperature for 45 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... SEAP expressing plasmid pSEAP was generated by substitution of eGFP coding sequence in pEGFP-C1 with sequence coding for SEAP in pLIVE® In Vivo Expression/Reporter Vectors (Mirus MIR 5620). SEAP integration into pSEAP plasmid sequence was checked by sequencing ...
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bioRxiv - Cell Biology 2019Quote: ... H3 and H424 were transfected into HEK293T cells together with lentiviral plasmid packaging mix using TransIT®-Lenti Transfection Reagent (Mirus #MIR 6600). The dual reporter cell line (cXG330 ...
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bioRxiv - Systems Biology 2021Quote: ... followed by incubation with tenfold diluted Label IT amine modifying reagent in 1× labelling buffer A from Label IT nucleic acid modifying reagent (Mirus Bio MIR 3900) at room temperature for 45 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 3×106 293T cells were plated in a 10cm dish and transfected 24h later with 2µg of the corresponding Src-Mpro-Tat-fLuc plasmid using TransIT-LT1(Mirus catalog number MIR 2304) transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded in 10-cm dishes or 6-well plates and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with EcoPack packaging plasmid and the MSCV-IRES-GFP (Empty backbone or MycWT or MycMUT) retroviral plasmids using TransIT®-LT1 (Mirus, MIR 2300) according to manufacturer instructions.
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bioRxiv - Cell Biology 2021Quote: ... the renilla luciferase reporter at 0,4 ng/mL and miRNA mimics at 10 nM using TransIT-X2 (Mirus Bio, Madison, WI, USA, #MIR 6000) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 293T ACE2 cells (gift of Michael Farzan) were transduced with pTRIP-SFFV-Hygro-TMPRSS2 using TransIT®-293 Transfection Reagent (Mirus Bio, MIR 2700) to obtain 293T ACE2/TMPRSS2 cells ...
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bioRxiv - Cell Biology 2023Quote: ... was then transfected into SH-SY5Y cells (50-60% confluent) in a 6-well plate using TransIT LT-1 reagent (Mirus Bio, Cat# MIR-2300). After 48 hr ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach 50%-70% confluency before transient transfection using TransIT-2020 (Mirus Bio, catalog #: MIR 5400) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2023Quote: ... were grown in 6-well plates and transfected the next day with 2.5 μg DNA with TransIT-LT1 (Mirus Bio catalog number MIR-2304) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the transfer plasmid and helper plasmids VSV-G and PSP (generously provided by David Sanders) were transfected into the Lenti-X cells using Transit293 transfection reagent (Mirus, Cat. No. MIR 2700) and incubated in OptiMEM (ThermoFisher Cat No ...
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bioRxiv - Biochemistry 2024Quote: ... they were transfected with 1800 ng of plasmid containing either N- or C-terminal tagged GFP-AMPKγ3 using TransITx2 transfection reagent (Mirus Bio, cat no. MIR 6000) and then lysed 48 hours later (lysis buffer – 50 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: Individual or pooled sgRNAs were introduced into CRISPRi- or CRISPRa-iPSCs via lentiviral delivery using TransIT Lenti Reagent (Mirus Bio LLC; Cat. No. MIR 6600) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: All transfections were performed on HEK 293T/17 cells in the presence of serum-free DMEM and Trans-IT transfection reagent (Mirus Bio, Madison, WI, #MIR 2305). All transductions were performed by spinoculation at 1100 x g for 30 min at 30°C.
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bioRxiv - Neuroscience 2023Quote: ... H1 plasmid library with the top 5 sgRNA per gene21 were transfected into HEK293s using the TransIT®-Lenti Transfection Reagent (Mirus, Cat. No. MIR 6606) along with third generation lentiviral packaging mix ...
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bioRxiv - Cell Biology 2024Quote: ... The human AD293 and iPSC cell lines were transfected using the Mirus TransIT®-LT1 Transfection Reagent (Cat # MIR 2300, Mirus Bio LLC, Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed with 2 × SSC three times and then the nucleic acids in the samples were amine-modified using a Label IT Amine modifying kit (Mirus Bio) for 30 min at 37 °C ...