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Citations for Mirus Bio :
51 - 100 of 236 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and 305 µL of TransIT-LT1 Transfection Reagent (Mirus Bio, MIR 2300) per T175 flask ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transfection of cells seeded in a T25 flask at a confluency of ≥80% was performed using TransIT®-CHO Transfection Kit (MIR 2174, Mirus Bio), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with siRNA using the TransIT-X2 System (Mirus, #MIR 600) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with FAM-labeled miR-214-3p using TransIT-X2 (Mirus). Live-cell images of mCherry-PrPC (red)-FAM-labeled miR-214-3p (green ...
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bioRxiv - Molecular Biology 2023Quote: ... by using Mirus TransIT-LT1 transfection reagent (Mirus Bio LLC, cat # MIR 2305). 24 h post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and standard packaging vectors using the TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). Supernatant was collected 48 hours post transfection ...
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bioRxiv - Microbiology 2023Quote: ... and 6 μl of TransIT-X2 Dynamic Delivery System (Mirus Bio; #MIR 6000). Following incubation at room temperature for 25 min ...
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bioRxiv - Genetics 2023Quote: ... using the TransIT Lentivirus transfection reagent (MIR 6600, Mirus Bio, Madison, WI, USA). gRNA libraries were designed from Brunello gRNAs in seven sub-pools with 4-gRNAs per gene and 5% Non-Target (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... 48 uL TransIT-293 transfection reagent (Mirus Bio LLC, catalog no. MIR 2704) diluted with 1300 uL serum-free media (SFM ...
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bioRxiv - Immunology 2022Quote: ... plasmid was fluorescently labeled with the Cy5 fluorophores using the Mirus Label IT® tracker intracellular nucleic acid localization kit (MIR 7021, Mirus Bio, Madison, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... Transfection of 293T and 293 cells was conducted using TransIT-2020 (Mirus, MIR 5400).
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bioRxiv - Cell Biology 2022Quote: ... Transfection were performed with the TransIT-X2 Transfection Reagent (Cat. MIR-6000, Mirus Bio). For CRISPR/Cas9-based knockdown of v-ATPase subunits ...
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bioRxiv - Neuroscience 2023Quote: ... using 25 µL TransIT®-LT1 Transfection Reagent (Mirus Bio, Cat. No. MIR 2300) in a final volume of 1mL DMEM medium supplemented with 10% tetracycline-free FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells expressing shRNA were transiently transfected using TransIT-LT1 reagent (Mirus, Cat# MIR-2300) in Opti-MEM (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.4μg TOB1-AS1 overexpression vector and 22.8μl TransIT-LT1 Transfection Reagent (Mirus, MIR 2306) were mixed and incubated at room temperature for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection solution contained 34.5 μl TransIT®-LT1 Transfection Reagent (Mirus, cat #MIR-2300), 1 μg transfer plasmid (psPAX.2) ...
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bioRxiv - Microbiology 2020Quote: ... or UL148HA were transfected using 9 μL of TransIT-2020 reagent (Mirus, Inc, #MIR 5404) into 6 × 105 HEK-293T cells that had been seeded into wells of a six-well cluster plate ...
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bioRxiv - Developmental Biology 2019Quote: All transfections for E14tg2a cells were performed using Trans IT-X2 reagent (Mirus, MIR 6003) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: All transfections were performed using 293T cells and TransIT-LT1 transfection reagent (Mirus, MIR 2305) according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... n=8 wells) complexed with 6 μL TransIT-2020 transfection reagent (Mirus Bio; MIR 5404) at a final DNA concentration of 8 ng/uL in Advanced DMEM (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... transfected with 3μg of total plasmid using TransIT-LT1 transfection reagent (Mirus, Cat: MIR 2306) following the manufacturer’s protocol with a TransIT-LT1 (μl) ...
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bioRxiv - Cell Biology 2023Quote: ... transfection of plasmid constructs was performed using Trans-IT 2020 reagent (Mirus Bio, #MIR 5400) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2024Quote: ... as described previously,17 using TransIT Lenti Transfection Reagent (Mirus Bio; Cat. No. MIR 6600) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected 24 hours after plating using the transfection reagent TransIT-LT1 (Mirus, MIR 2305) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The siRNA molecules were transfected using a TransIT-X2 Dynamic Delivery System (Mirus cat. MIR 6000).
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bioRxiv - Developmental Biology 2023Quote: ... This RNA was then introduced to Vero cells using TransIT-mRNA transfection (Mirus Bio, MIR 2250). At 72 hours post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2 cells were transfected with lentiviral DNA following the Mirus-LT1 (Mirus MIR 2300) transfection protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Cell Biology 2021Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Genomics 2021Quote: ... The cells were then transfected with TransIT Lenti-transfection reagent (MIR 6600, Mirus Bio, Madison, WI, USA) using an envelope plasmid (pVSVg ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral supernatants were generated by co-transfection of HEK-293T cells using Transit-X2 (Mirus; #MIR 6004) with a 3-vector lentiviral system ...
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bioRxiv - Cell Biology 2022Quote: ... Szczesny) and 0.8 μg EGFP-CHIP plasmid using Mirus reagents: 2 ul TransIT-293 (MIR 2700, Mirus) for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900 ...
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bioRxiv - Bioengineering 2020Quote: ... Transient transfection of HEK293T cells was performed using TransIT®-293 Transfection Reagent (Mirus Bio, MIR 2700).
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bioRxiv - Synthetic Biology 2023Quote: ... and target luciferase using hydrodynamic tail vein injection with TransIT-EE Delivery Solution (Mirus Bio MIR 5340). Mice were under anesthesia during injections ...
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bioRxiv - Cancer Biology 2023Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Genetics 2023Quote: ... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25% ...
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bioRxiv - Genetics 2023Quote: ... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v3 lentiviral sgRNA library at a target infection rate of 10% ...
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bioRxiv - Cancer Biology 2020Quote: ... 7.5ug PsPax2 and 2.5ug VSV-G using TransIT-LT1 transfection reagent via manufacturer instructions (Mirus; Cat#: MIR 2305). FUGW (Addgene ...
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bioRxiv - Microbiology 2020Quote: ... a 10x mix was prepared in OptiMEM containing siRNA(s) and TransIT-X2 transfection reagent (MIR 600x, Mirus) in a 1:2 stoichiometry ...
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bioRxiv - Cell Biology 2022Quote: ... Lentivirus was made by transfecting Lx293T with standard packaging vectors and TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). Viral supernatant was harvested two days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Electroporation was performed using a Gene Pulser XCell in 600 µL Ingenio electroporation buffer (Mirus Bio; MIR 50114) with the settings of 260V & 500 μF using an exponential decay in a 0.4 cm cuvette (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... in combination with 3μg psPAX2 (packaging plasmid) and 2μg pMD2.G (envelope plasmid) with 30μL TransIT-LT1 transfection reagent (Mirus, MIR 2300). For T-ALL cell infection ...
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bioRxiv - Cell Biology 2020Quote: ... 150 μL pre-warmed OPTI-MEM was placed in 1.5 mL tubes with 6 μL TransIT-LT1 transfection reagent (MIR 2300, Mirus), vortexed briefly ...
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bioRxiv - Cell Biology 2022Quote: ... for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900, Mirus) for HeLa Flp-In T-REx cells ...
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bioRxiv - Biophysics 2023Quote: ... STARD11-GFP WT or mutant plasmids were transfected using the TransIT-X2® Transfection Reagent (Mirus Bio, MIR 6000). Cells were fixed in 3% paraformaldehyde for 20 min before staining with antibodies diluted in a PBS solution of 1% BSA and 0.05% saponin ...
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bioRxiv - Cell Biology 2023Quote: ... and gRNA plasmids for targeting PRKAA1 and PRKAA2 were co-transfected into cells with TransIT-LT1 (Mirus, MIR 2305) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and pMD2.G at a ratio of 9:8:1 by mass using TransIT-LT1 transfection reagent (Mirus MIR 2306) at a ratio of 3 μL transfection reagent per 1 μg plasmid ...
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bioRxiv - Microbiology 2021Quote: ... a 10x mix was prepared in OptiMEM containing the appropriate siRNA(s) and TransIT-X2 transfection reagent (MIR 600x, Mirus) in a 1:2 stoichiometry ...