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Citations for Mirus Bio :
1 - 50 of 83 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... using TransIT reagent (0.25 µl/well, 96-well plate, and 1 µl/well, 48-well plate, Mirus Bio). The transfection mix was replaced with fresh medium after 4 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... reporter plasmid delivery (3 µg/plate for cFSHr and 0.3 µg/plate for cLHr) was carried out with TransIT-X2® System (Mirus). The cells were serum-starved for 16 h ...
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bioRxiv - Cancer Biology 2022Quote: ... in 6-well plates by using TransIT-293 transfection reagent (Mirus #MIR2705) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: U2OS cells were plated on the glass-based dish (Matsunami) and transfected with the EKAREV plasmid using TransIT-LT1 reagent (Mirus) according to the manufacture’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 106 HEK293T cells seeded in 100mm plates were cotransfected (TransIT, Mirus BIO 2700) with 6.5 µg of psPAX2 (Addgene 12260) ...
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bioRxiv - Biochemistry 2024Quote: ... and transfections were performed in 6-well plates with TransIT-293 (Mirus Bio) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: Transfection of constructs was performed in 12-well plates using TransIT-LT1 reagent (Mirus) with 1 μg DNA split evenly among plasmids used in each transfection ...
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bioRxiv - Immunology 2021Quote: ... Retroviruses were packaged in PlatE cells by transient transfection using TransIT 293 (Mirus Bio) or Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: Cells were plated on 96 well plates and transfected using the TransIT-X2 (Mirus) transfection reagent with 0.075 µg DNA per well according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and PEX19 sgRNAs were transfected in 96-well plates by lipofectamine (TransIT LT-1, Mirus). A normalized 55 ng of total plasmid DNA was used at a ratio of 50:5 TOPFlash/FOPFlash:Renilla ...
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bioRxiv - Biochemistry 2023Quote: Ptch1−/− MEFs were seeded in 24-well plates and transfected using TransIT-X2® (Mirus) and Opti-MEM reduced serum media (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded into 96-well plates the day before transfecting with TransIT Pro (Mirus). Transfections were performed using 10ng/well of firefly expression plasmid and 5ng/well of PUb-RL Renilla luciferase normalization control plasmid (17) ...
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bioRxiv - Immunology 2021Quote: ... U2OS-STING cells plated in 12-well plates were transiently transfected using TransIT®-2020 (Mirus) with 500 ng of ATAD3A WT ...
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bioRxiv - Genomics 2023Quote: ... on Matrigel (Corning)-coated plates using a reverse transfection method and Mirus TransIT-LT1 reagent (Mirus Bio, Madison, WI) and transfected cells were transiently selected for puromycin resistance ...
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bioRxiv - Bioengineering 2022Quote: ... The vectors were transfected into A549 cells in 96-well microtiter plate using TransLT transfection reagent (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 0.08 million cells/well were seeded in a 24 well plate and transfected with TransIT-293 (Mirus) with 0.5µg/well of either pTRIP-SFFV-Hygro-2A-mScarlet ...
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bioRxiv - Microbiology 2024Quote: Huh7.NTCP cells seeded in a 48-well plates were transfected using TransIT-LT1 transfection reagent (Mirus) and lysed 72 hours later using the Bright-Glo Luciferase reagent (Promega) ...
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bioRxiv - Immunology 2021Quote: HEK 293T cells (106) were transfected in 6-well plates with corresponding plasmids with Transit LT1 transfection reagent (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Preadipocytes were transfected by seeding 30 000 cells per well of a 24 well plate in growth medium and adding 1.5 μl TransIT-X2 (Mirus) and 125 ng plasmid DNA or 1.4 μl LNA (10 μM ...
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bioRxiv - Microbiology 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Biophysics 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Microbiology 2022Quote: ... monolayers of BHK-T7 cells (4×105) cultured in 12-well plates were co-transfected using 2.5 μl of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Microbiology 2022Quote: ... purified replicon RNA was transfected into HeLa cells grown on 96 well plates using TransIT mRNA transfection reagent (Mirus), and the cells were incubated in the growth medium supplemented with 5µM of cell-permeable Renilla luciferase substrate EnduRen (Promega ...
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bioRxiv - Microbiology 2020Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Immunology 2022Quote: Viral particles were produced by transfection of HEK293FT cells in 6-well plates with 3μg DNA and 8μL TransIT-293 (Mirus Bio) per well ...
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bioRxiv - Immunology 2023Quote: 293T cells were seeded in 6-well plates at 0.2M cells/ml and were transfected with TransIT-LT1 (Mirus) 24h later with 1500 ng of empty plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... in a 12-well plate overnight and plasmids (1.5 μg) were transfected using 3 μl of TransIT-LT1 (Mirus). For IP experiments ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected in duplicate into HEK293T cells in a 48-well plate using LT-1 transfection reagent (Mirus) with the indicated plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... cells were split 1:2 onto pre-coated 12-well plates and 4 h later were transfected with 500 ng ERGFP_NS using TransIT-293 Transfection Reagent (Mirus). Media was changed after 24 h ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 ESCs were nucleofected with 4ug linearized 2C-GFP plasmid and plated at low density in 10cm2 plates then selected with 250 μg/mLG418 (Mirus) for 8 days ...
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bioRxiv - Cell Biology 2020Quote: ... Neuron progenitors were cultured on PO/LA coated plate and transfected with the RFP-LEPR carrying plasmid using the TransIT-Neural transfection reagent (Mirus) for 48 hours before leptin treatment ...
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bioRxiv - Microbiology 2022Quote: HEK293T cells were seeded at 1.5-2 × 105 cells/well in 24-well plates the day before transfection using TransIT-LT1 reagent at 1.5μL transfection reagent/well (Mirus Bio LLC). 100 ng of indicated constructs encoding a N-terminal mCherry tagged CARD8 were co-transfected into HEK293T cells with either 400 ng of pcDNA3.1 empty vector (‘–‘) ...
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bioRxiv - Immunology 2022Quote: H1 control AC16 cardiomyocytes and cGAS KO AC16s were seeded in 12-well plates and transfected with 5 mg of CT-DNA using Transit X2 (Mirus) at a 2:1 ratio for 8 h prior to harvest ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 6 well plates (2.0×105 per well) and incubated overnight before transfection with pCAGGS-VP40 using lipofectamin LT-1 (MIR2304; Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 25,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx using TransIT-X2 (MIR 6003, Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in a 100 mm plate overnight and plasmids (15 μg) were transfected using 45 μl of TransIT-LT1 (Mirus). The cells were cultured for 48 h ...
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bioRxiv - Microbiology 2023Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... virus was produced using Lenti-X 293T cells in 24-well plates using TransIT LT1 (Mirus Bio LLC #MIR 2305) and second-generation packaging vectors pMD2.G and psPAX2 according to standard lentiviral production protocols.
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bioRxiv - Genomics 2024Quote: ... mRNA transfections were performed using 0.5 µg of mRNA in 6-well plates using TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells in 6-well plates were transiently transfected with 1 µg of PSMB-3xFLAG constructs using TransIT-293 transfection reagent (Mirus). To analyze interactions between KCMF1-3xFLAG mutants and endogenous proteins ...
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bioRxiv - Cell Biology 2022Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg of total DNA and 8 mL TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Genomics 2022Quote: ... in HK-2 human proximal tubule cells at approximately 70% confluency in 96-well plates by using TransIT-2020 Reagent (Mirus, #5404), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and NP proteins (23) were transfected into 50% confluent HEK293T cells in 6-well plates with TransIT-LT1 transfection reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg DNA and 8 μl TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Microbiology 2020Quote: ... Confluent monolayers of BHK-T7 cells in 12-well plates were co-transfected using TransIT-LT1 transfection reagent (Mirus Bio LLC) with 0.27 μg of each of eleven plasmid constructs representing the SA11 rotavirus genome and of each of two plasmids encoding vaccinia virus mRNA capping enzymes ...
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bioRxiv - Microbiology 2022Quote: ... were seeded into 6-well plates and transfected the next day with 200 ng pcDNA4/TO-RNR-3×FLAG using TransIT-LT1 (Mirus #2304). A3B-EGFP or A3A-EGFP expression was induced 6 hours after transfection with 50 ng/mL doxycycline ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 μg pVSVG per 1 reaction in a 6-well plate well) into HEK 293T cells with TransIT-LT1 transfection reagent (Mirus #2304) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... BHK-21 cells cultured in 12-well plates were transfected with 3 μg capped in vitro transcribed DENV2 RNAs using the TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions ...