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Citations for Mirus Bio :
51 - 100 of 154 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 48 uL TransIT-293 transfection reagent (Mirus Bio LLC, catalog no. MIR 2704) diluted with 1300 uL serum-free media (SFM ...
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bioRxiv - Neuroscience 2023Quote: ... XGal staining was performed using the beta-galactosidase staining kit (Mirus #MIR 2600), following the provided protocol ...
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bioRxiv - Genomics 2019Quote: ... The plasmids were electroporated into K562 cells with Ingenio Electroporation Kit (Mirus, MIR 50115). For each electroporation ...
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bioRxiv - Microbiology 2019Quote: ... Transfection of 293T and 293 cells was conducted using TransIT-2020 (Mirus, MIR 5400).
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bioRxiv - Cell Biology 2022Quote: ... Transfection were performed with the TransIT-X2 Transfection Reagent (Cat. MIR-6000, Mirus Bio). For CRISPR/Cas9-based knockdown of v-ATPase subunits ...
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bioRxiv - Neuroscience 2023Quote: ... using 25 µL TransIT®-LT1 Transfection Reagent (Mirus Bio, Cat. No. MIR 2300) in a final volume of 1mL DMEM medium supplemented with 10% tetracycline-free FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells expressing shRNA were transiently transfected using TransIT-LT1 reagent (Mirus, Cat# MIR-2300) in Opti-MEM (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.4μg TOB1-AS1 overexpression vector and 22.8μl TransIT-LT1 Transfection Reagent (Mirus, MIR 2306) were mixed and incubated at room temperature for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection solution contained 34.5 μl TransIT®-LT1 Transfection Reagent (Mirus, cat #MIR-2300), 1 μg transfer plasmid (psPAX.2) ...
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bioRxiv - Microbiology 2020Quote: ... or UL148HA were transfected using 9 μL of TransIT-2020 reagent (Mirus, Inc, #MIR 5404) into 6 × 105 HEK-293T cells that had been seeded into wells of a six-well cluster plate ...
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bioRxiv - Developmental Biology 2019Quote: All transfections for E14tg2a cells were performed using Trans IT-X2 reagent (Mirus, MIR 6003) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: All transfections were performed using 293T cells and TransIT-LT1 transfection reagent (Mirus, MIR 2305) according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... n=8 wells) complexed with 6 μL TransIT-2020 transfection reagent (Mirus Bio; MIR 5404) at a final DNA concentration of 8 ng/uL in Advanced DMEM (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... transfected with 3μg of total plasmid using TransIT-LT1 transfection reagent (Mirus, Cat: MIR 2306) following the manufacturer’s protocol with a TransIT-LT1 (μl) ...
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bioRxiv - Cell Biology 2023Quote: ... transfection of plasmid constructs was performed using Trans-IT 2020 reagent (Mirus Bio, #MIR 5400) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2024Quote: ... as described previously,17 using TransIT Lenti Transfection Reagent (Mirus Bio; Cat. No. MIR 6600) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected 24 hours after plating using the transfection reagent TransIT-LT1 (Mirus, MIR 2305) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA-RNA was covalently labeled with Cy3 using a LabelT-Cy3 kit (Mirus Bio MIR 3625) following the manufacturer instructions.
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bioRxiv - Neuroscience 2022Quote: ... was transfected into BHK cells with TransIT-mRNA Transfection Kit (Mirus Bio, Cat. No. MIR 2225). Virus containing supernatant was concentrated ...
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bioRxiv - Immunology 2020Quote: ... The siRNA molecules were transfected using a TransIT-X2 Dynamic Delivery System (Mirus cat. MIR 6000).
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bioRxiv - Cell Biology 2021Quote: ... 5 µg pre-miR-181a-1 were labeled with cy3 using Nucleic Acid Labeling Kit (Mirus) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... This RNA was then introduced to Vero cells using TransIT-mRNA transfection (Mirus Bio, MIR 2250). At 72 hours post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2 cells were transfected with lentiviral DNA following the Mirus-LT1 (Mirus MIR 2300) transfection protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Cell Biology 2021Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Genomics 2021Quote: ... The cells were then transfected with TransIT Lenti-transfection reagent (MIR 6600, Mirus Bio, Madison, WI, USA) using an envelope plasmid (pVSVg ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral supernatants were generated by co-transfection of HEK-293T cells using Transit-X2 (Mirus; #MIR 6004) with a 3-vector lentiviral system ...
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bioRxiv - Microbiology 2022Quote: ... or BVDV IRES-driven Fluc mRNA reporters using TransIT®-mRNA Transfection Kit (Mirus Bio MIR 2250) at a 1:2 (µg to µl ...
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bioRxiv - Cell Biology 2022Quote: ... Szczesny) and 0.8 μg EGFP-CHIP plasmid using Mirus reagents: 2 ul TransIT-293 (MIR 2700, Mirus) for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900 ...
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bioRxiv - Bioengineering 2020Quote: ... Transient transfection of HEK293T cells was performed using TransIT®-293 Transfection Reagent (Mirus Bio, MIR 2700).
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bioRxiv - Synthetic Biology 2023Quote: ... and target luciferase using hydrodynamic tail vein injection with TransIT-EE Delivery Solution (Mirus Bio MIR 5340). Mice were under anesthesia during injections ...
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bioRxiv - Cancer Biology 2023Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Genetics 2023Quote: ... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25% ...
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bioRxiv - Genetics 2023Quote: ... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v3 lentiviral sgRNA library at a target infection rate of 10% ...
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bioRxiv - Cancer Biology 2020Quote: ... 7.5ug PsPax2 and 2.5ug VSV-G using TransIT-LT1 transfection reagent via manufacturer instructions (Mirus; Cat#: MIR 2305). FUGW (Addgene ...
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bioRxiv - Microbiology 2020Quote: ... a 10x mix was prepared in OptiMEM containing siRNA(s) and TransIT-X2 transfection reagent (MIR 600x, Mirus) in a 1:2 stoichiometry ...
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bioRxiv - Cell Biology 2022Quote: ... Lentivirus was made by transfecting Lx293T with standard packaging vectors and TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). Viral supernatant was harvested two days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Electroporation was performed using a Gene Pulser XCell in 600 µL Ingenio electroporation buffer (Mirus Bio; MIR 50114) with the settings of 260V & 500 μF using an exponential decay in a 0.4 cm cuvette (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... in combination with 3μg psPAX2 (packaging plasmid) and 2μg pMD2.G (envelope plasmid) with 30μL TransIT-LT1 transfection reagent (Mirus, MIR 2300). For T-ALL cell infection ...
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bioRxiv - Cell Biology 2020Quote: ... 150 μL pre-warmed OPTI-MEM was placed in 1.5 mL tubes with 6 μL TransIT-LT1 transfection reagent (MIR 2300, Mirus), vortexed briefly ...
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bioRxiv - Cell Biology 2022Quote: ... for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900, Mirus) for HeLa Flp-In T-REx cells ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... T7 synthesized mRNA was transfected in cells at 70-80% confluency with TransIT-mRNA Transfection Kit (Mirus, MIR 2256) following the manufacturer’s recommended protocol ...
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bioRxiv - Biophysics 2023Quote: ... STARD11-GFP WT or mutant plasmids were transfected using the TransIT-X2® Transfection Reagent (Mirus Bio, MIR 6000). Cells were fixed in 3% paraformaldehyde for 20 min before staining with antibodies diluted in a PBS solution of 1% BSA and 0.05% saponin ...
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bioRxiv - Cell Biology 2023Quote: ... and gRNA plasmids for targeting PRKAA1 and PRKAA2 were co-transfected into cells with TransIT-LT1 (Mirus, MIR 2305) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and pMD2.G at a ratio of 9:8:1 by mass using TransIT-LT1 transfection reagent (Mirus MIR 2306) at a ratio of 3 μL transfection reagent per 1 μg plasmid ...
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bioRxiv - Microbiology 2021Quote: ... a 10x mix was prepared in OptiMEM containing the appropriate siRNA(s) and TransIT-X2 transfection reagent (MIR 600x, Mirus) in a 1:2 stoichiometry ...
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were transfected using the Mirus TransIT®-LT1 Transfection Reagent (Cat# MIR-2304, Mirus Bio LLC, Madison, WI, USA) with the pSpCas9(BB)-2A-GFP plasmid (Cat# 48138 ...
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bioRxiv - Neuroscience 2023Quote: ... and 20.5 µg pcDNA3.1 VSV-G in 4.9 ml DMEM with 345 µl TransIT-Lenti Transfection Reagent (Mirus Bio, MIR 6600) added to 4.8 ml DMEM ...