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Citations for Mirus Bio :
51 - 100 of 118 citations for Tonsoku Like DNA Repair Protein TONSL Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 7 μg of DNA was mixed with 21 μL of Trans-IT LT1 transfection reagent (Mirus) or LipoD transfection reagent and 1750 μL DMEM and incubated at RT for 20 minutes to allow formation of DNA/transfection reagent complexes before addition to the cells ...
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bioRxiv - Cancer Biology 2022Quote: ... The transient plasmid DNA transfection was performed using TransIT®-LT1 Transfection Reagent (Mirus, Madisom, WI). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... we performed forward transfection by incubating DNA with TransIT-LT1 Transfection Reagent (Mirus Bio, Madison, WI) in a 1:3 DNA:reagent ratio in Opti-MEM for 15–20 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2 cells were transfected with lentiviral DNA following the Mirus-LT1 (Mirus MIR 2300) transfection protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 1.5 µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167 µg of pMD2.G and 1.3 µg of p8.91) with TransIT-293 (Mirus Bio). After 2–3 d of transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Molecular Biology 2022Quote: HDRT and xHDRT DNA were Cy5-labeled using the Label IT® Nucleic Acid Labeling Reagents (Mirus) and used in a standard nucleofection protocol (see Cas9 ...
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bioRxiv - Microbiology 2020Quote: ... All transfections used 2.49 μg plasmid DNA with 6.25 μL TransIT LT1 transfection reagent (Mirus, Madison, WI) in 250 μL Opti-MEM (Gibco).
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bioRxiv - Microbiology 2019Quote: ... All transfections used 2.49 μg plasmid DNA with 6.25 μL TransIT LT1 transfection reagent (Mirus, Madison, WI), in 250 μL Opti-MEM (Gibco) ...
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bioRxiv - Systems Biology 2023Quote: The DNA library was co-transfected with pCMV-dR8.91 and pMD2.G plasmids using TransIT-Lenti (Mirus) into HEK293 cells ...
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bioRxiv - Microbiology 2023Quote: ... All transfections used 2.49 µg plasmid DNA with 6.25 µL TransIT LT1 transfection reagent (Mirus, Madison, WI) in 250 µL Opti-MEM (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells containing RNP and donor DNA were electroporated at 150V using the Ingenio EZporator Electroporation System (Mirus). Cells were incubated at ambient temperature for 5 min and transferred to a six-well plate containing 2 mL growth medium with or without combinations of 1 μM AZD7648 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5 million MEFs and 5µg total DNA were diluted in 100µL of Ingenio Solution in a 0.2cm cuvette (Mirus). MEFs were nucleofected using AMAXA program A-023 (MEF) ...
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bioRxiv - Immunology 2023Quote: ... These DNA mixes were incubated with 18 mL OptiMEM and 1 mL of TransIT-LT1 reagent (Mirus) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells (150 cm2 dish) were transfected with 6 μg of each protein-coding plasmid by using TransIT (Mirus). Cell lysis and elution were performed as described above ...
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bioRxiv - Developmental Biology 2021Quote: ... MmAtxn10::EGFP-N1 plasmids were transfected into cells using TransIT®-2020 DNA per manufacturer guidelines (Mirus, MIR5404).
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bioRxiv - Microbiology 2020Quote: ... Cells were transiently transfected with 500ng of total DNA and 1.5μl of Transit X2 (Mirus Bio, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 10g of plasmid DNA was transfected into 293T lentiviral packaging cells using LT1 transfection reagent (Mirus Bio LLC), and viral supernatants were collected ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 2 μg of DNA 24 - 48 hours before measurement using TransIT transfection reagents (Mirus). DRG neurons from adult (postnatal weeks 8–12 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 10 µg of DNA at 70-80% cell density using TransIT-LT1 (Mirus, 2300) for 293T cells and at 50-60% cell density with Lipofectamine 3000 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells in 6 well plates were transfected with 500 ng/well of each protein-coding plasmid by using TransIT (Mirus). After 48 h ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... Constructs were mixed with 0.5μg helper (encoding gag/pol) and 0.1μg encoding coat protein (pMD2) along with 6μL TransIT-Lenti transfection reagent (MIR6603; Mirus Bio; Madison, WI) in 200uL Opti-MEM (31985062 ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcribed full-length CHIKV mRNA and mRNA encoding single viral proteins was transfected into target cells with the TransIT mRNA kit (Mirus). MAYV (strain TRVL15537 ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr ...
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bioRxiv - Systems Biology 2021Quote: ... and grown in complete media for 1 day before co-transfection of the mammalian expression vectors encoding GFP and viral protein of interest using TransIT-2020 transfection reagent (#MIR5404, Mirus). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in RPE1 or HeLa cells for 24–48 h using TransIT®-LT1 Transfection Reagent (Mirus Bio) or Neon Electroporation (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... Virus-like particles (VLPs) packaging Vpr WT or mutant proteins were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). VLPs were harvested 48 hrs post transfection ...
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bioRxiv - Immunology 2019Quote: Viral particles were produced by transfection of HEK293FT cells in 6-well plates with 3µg DNA and 8µl TransIT-293 (Mirus Bio) per well ...
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bioRxiv - Immunology 2022Quote: Viral particles were produced by transfection of HEK293FT cells in 6-well plates with 3μg DNA and 8μL TransIT-293 (Mirus Bio) per well ...
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bioRxiv - Cell Biology 2020Quote: Transient DNA transfections of wild-type AP4B1 were carried out using a TransIT-HeLaMONSTER® kit (Mirus Bio LLC), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and transfected with 1.5 µg of the indicated plasmid DNA and 0.5 µg of the PiggyBac transposase construct using TransIT-X2 (MIR6004, Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... the cells were plated in a 12.5 cm2 dish and were transfected at 60% confluency with 0.8 µg DNA using TransIT-293 (Mirus, MIR2700). We used a GFP-tagged and C-terminally truncated human HCN1 construct which was cloned into a pEG plasmid as previously reported by Lou & MacKinnon (2017) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of total plasmid DNA and 1.5 μL of TransIT ®-2020 Transfection Reagent (Mirus Bio, WI, USA) were mixed by gentle pipetting ...
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bioRxiv - Cancer Biology 2023Quote: ... were done by mixing 2.5 µg of plasmid DNA with 7.5 µl of TransIT-X2 reagent (Mirus, Madison, WI). The mixture was added to 105 CAF or PDAC cells cultured in serum-free and antibiotic-free media overnight.
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bioRxiv - Biochemistry 2024Quote: ... and TRUPATH plasmids at 1:1:1 DNA ratio (Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Immunology 2022Quote: ... and NP proteins (23) were transfected into 50% confluent HEK293T cells in 6-well plates with TransIT-LT1 transfection reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with 250 ng of a pCDNA3.1(+) vector encoding for the SARS-CoV-2 spike protein using the TransIT®-LT1 Transfection Reagent (Mirus Bio). Six hours post transfection (p.t.) ...
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bioRxiv - Cell Biology 2022Quote: ... cells in each well were transfected with a plasmid encoding GFP-2A-POI (protein of interest) using TransIT-2020 transfection reagent (Mirus #5404) as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... and rev genes and pMD2.G encoding the VSV-G envelope protein using the TransIT-Lenti transfection reagent (Mirus, MIR 6650). The virus was harvested after 48 hours and filtered through a 0.45 μm filter ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at ∼60% confluency with 2.5 µg of the appropriate DNA construct using the TransIT-LT1 Transfection Reagent (Mirus; MIR2300) and cultured for an additional 2 days ...
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bioRxiv - Microbiology 2019Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
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bioRxiv - Microbiology 2019Quote: ... MHV68 was produced by first making p0 virus by transfecting NIH 3T3 cells in 6-well plates with 2.5 μg BAC DNA using TransIT-X2 (Mirus Bio) for 24h ...
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bioRxiv - Neuroscience 2021Quote: TDP-43 inducible knockdown SH-SY5Y cells were electroporated with 2 μg of DNA with the Ingenio electroporation kit (Mirus) using the A-023 setting on an Amaxa II nucleofector (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were transiently transfected with 500 ng of total DNA and 1.5 µl of Transit X2 (Mirus Bio, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: H1 control AC16 cardiomyocytes and cGAS KO AC16s were seeded in 12-well plates and transfected with 5 mg of CT-DNA using Transit X2 (Mirus) at a 2:1 ratio for 8 h prior to harvest ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 250 ng of DNA (split evenly by mass between the plasmids indicated) using TransIT-LT1 (Mirus). Cells were either incubated for 24 h (untreated) ...