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Citations for Mirus Bio :
151 - 200 of 299 citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL and psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL plasmids were transfected into cells with TransIT-293 Reagent (Mirus, MIR2704). After 24 h of incubation ...
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bioRxiv - Cell Biology 2021Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio, MIR 2705). Following transfection ...
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bioRxiv - Microbiology 2020Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg DNA and 8 μl TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Cell Biology 2021Quote: ... C-terminal GFP-2x Strep-tagged full length and truncation versions of CEP90 were cloned into pLVX-EF1α-IRES-Puro backbone and transfected into 293T cells using TransIT-293 transfection reagent (Mirus Bio) according to manufacturer guidelines.
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral vector was produced by transforming the produced LARRY plasmid into HEK293T cells using the Trans-IT 293 transfection reagent (Mirus #MIR2700). For each of eighteen 10 cm dishes of HEK293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... carrying the sequence of the gene to be over expressed and resistance to puromycin into 0.8×106 HEK293 cells in a 60 mm culture dish with 6 µl of TrasnIT (Cat. Nº: Mirus Bio. 293) containing 3 ml of complete media ...
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bioRxiv - Microbiology 2023Quote: ... were cotransfected with 1 μg psPAX2-IN/HiBiT,23 1 μg pWPI-Luc2,23 and 500 ng plasmids expressing parental S or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 7 and 10 in this background were obtained by transfecting the adherent parental cell line with PX459 encoding the respective sgRNA using TransIT-293 transfection reagent (Mirus, USA). 48 h post transfection ...
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bioRxiv - Neuroscience 2024Quote: ... cells in one 35 mm dish were either transiently transfected with the construct to be imaged using TransIT-293 lipofection reagent (Mirus Bio) or were virally transduced with a lentivirus ...
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bioRxiv - Immunology 2022Quote: ... and transfer plasmids were co-transfected into the packaging cell line HEK-293T (clone 17; ATCC) using TransIT-LT1 (Mirus, MIR2305). Media containing the lentivirus was collected after transfection ...
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bioRxiv - Microbiology 2019Quote: ... ZIKV was rescued by transfection of the infectious clone in semi-confluent HEK-293T cells using TransIT-LT1 transfection reagent (Mirus Bio). For both viruses ...
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bioRxiv - Microbiology 2019Quote: ... and varying input (50 ng for 5-site screen and 15 ng for 4-site screen) of MxA plasmids were co-transfected into HEK 293T cells using the reagent TransIT-LT1 (Mirus Bio) in a 96-well plate format ...
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bioRxiv - Neuroscience 2024Quote: ... and maintained in the media described above supplemented with either 1.5ug/mL (HEK-293T and HeLa) or 10µg/mL puromycin (MDCK) (Mirus Bio, MIR 5940). A clonal MDCK-ALPL line with high transcytosis activity (MDCK-ALPL clone 21 ...
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bioRxiv - Molecular Biology 2023Quote: ... the generated vectors and the PIK packaging plasmid were delivered into HEK 293T cells using the TransIT-LT1 transfection reagent (Mirus Bio). Stable MEF cell lines expressing either wild-type (WT ...
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bioRxiv - Cell Biology 2023Quote: ... the packaging cell line HEK293-FT was transfected with pTCP-MPsfGFP together with pSAX2 (12260 Adgene) and pCMV-VSV-G (8454 Adgene) plasmids using the kit TransIT®-293 Transfection Reagent (Mirus Bio) and following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg pWPI-Luc2 (a reporter plasmid encoding a firefly luciferase gene14) and 500 ng plasmids expressing parental S protein or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) or TransIT-LT1 (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... TANGO1ShortΔPRD-mCh-ILID-pHRSIN and EGFP-SspB-Sec23A-pHRSIN were separately added to 8× 105 HEK−293T cells with a packaging vector pool (comprising of pCMV 8.91 and pMDG) using TransIT®-293 transfection reagent (Mirus Bio LLC). Post transfection cells were incubated ...
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bioRxiv - Genetics 2019Quote: ... Y15F and Y15F mutant cDNA were generated as described above and transfected into HEK-293T cells (ATCC; CRL-3216) using TransIT®-LT1 Transfection Reagent (Mirus; MIR2305) following user instructions ...
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bioRxiv - Genetics 2020Quote: ... shUBR5 and shScrambled-pLKO.1-puro were co-transfected with pCMV-VSV-G and pCMV-dR8.2 dvpr plasmids into HEK 293T cells using TransIT293 reagent (Mirus Bio LLC, USA). To generate stable silenced shUBR5 cells ...
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bioRxiv - Neuroscience 2019Quote: ... VSV-G pseudotyped lentiviral vectors were produced by transfection of human embryonic kidney cells (HEK293FT) with third-generation lentivirus plasmids using lipofection (Mirus TransIT®-293). Supernatant was collected 48 h after transfection and concentrated using ultrafiltration (Centricon Plus-20 PLGC centrifuge filter units).
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bioRxiv - Systems Biology 2020Quote: ... 293T ACE2 cells (gift of Michael Farzan) were transduced with pTRIP-SFFV-Hygro-TMPRSS2 using TransIT®-293 Transfection Reagent (Mirus Bio, MIR 2700) to obtain 293T ACE2/TMPRSS2 cells ...
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bioRxiv - Immunology 2023Quote: All transfections were performed on HEK 293T/17 cells in the presence of serum-free DMEM and Trans-IT transfection reagent (Mirus Bio, Madison, WI, #MIR 2305). All transductions were performed by spinoculation at 1100 x g for 30 min at 30°C.
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bioRxiv - Cell Biology 2023Quote: ... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Microbiology 2021Quote: ... and RdRp-N462 or RdRp-D462 were transfected into 293T cells at a 1:1:1 ratio using TransIT LT-1 (Mirus, Madison, WI). To normalize transfection efficiency ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentivirus expressing RIPK3 shRNA was generated by transfecting HEK-293T cells in 6 well plate with a 1: 0.1: 1 ratio of pMDG2: pVSVG: pLKO.1 with TransIT-LT1 transfection reagent (Mirus). After filtering through 0.45 μm of cellulose acetate membrane (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... and TRUPATH plasmids at 1:1:1 DNA ratio (Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Cancer Biology 2021Quote: ... and RSV-REV in the ratio of (3:1:1:1) into HEK293T cells using Trans-IT transfection reagent (Mirus). Virus-rich supernatant was collected at 72 hr post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µg of each sgRNA plasmid and 3 µg of dCas9-KRAB-MeCP2 plasmid were co-nucleofected into 1 × 106 mHypoA cells resuspended in a 1:1 mixture of Ingenio Electroporation reagent (Mirus Bio 50111) and OptiMEM (Gibco 31985062) ...
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bioRxiv - Microbiology 2024Quote: ... pVSV-G and either pLRGatewayIRESeFYP-Vpr/Vpx or pLRGatewayIRESmApple-Vpr/Vpx at a ratio 1:1:1 with TransIT-lenti (Mirus, MIR 6603). To generate HIV vectors to make stable cell lines ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 μl TransIT (Mirus Bio TransIT-mRNA transfection kit ...
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bioRxiv - Microbiology 2020Quote: ... or Transit LT-1 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... or Transit LT-1 (Mirus).
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bioRxiv - Microbiology 2022Quote: ... 1 µl of mRNA Boost Reagent and 1 µl of TransIT-mRNA Reagent (Mirus Bio) and incubated for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 at a ratio of 1:1:0.1 using TransIT-LT1 transfection reagent (Mirus). The resulting blend was then filtered through a cellulose acetate membrane (0.45 µm ...
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bioRxiv - Neuroscience 2023Quote: ... and pHR: mU6-sgRNA-EF1A-puro-P2A-BFP (sgRNA-BFP) (1:1:1) using 24 μL TransIT®-LT1 Transfection Reagent (Mirus Bio, Cat. No. MIR 2300) in a final volume of 1000uL Opti-MEM medium (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Bait and prey plasmids containing fused fragments were then individually transfected together with VSVG and gag/pol packaging vectors in a ratio of 3:1:1 into HEK293T cells using Trans-IT Transfection reagent (Mirus) to produce retroviruses ...
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bioRxiv - Immunology 2021Quote: ... transfected with 44 μg plasmid encoding SARS-CoV-2 Spike (pCG1-SARS-2-S, Wuhan Hu-1) using Transit LT-1 (Mirus). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using TransIT-LT-1 (Mirus) transfection reagent and 2500ng plasmid/flask ...
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bioRxiv - Cell Biology 2023Quote: ... and pMDLg/pRRE) at a 1:1 ratio and transfected into HEK293T cells using TransIT LT1 transfection reagent (Mirus Bio LLC). After 72 h virus-containing supernatants were collected ...
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bioRxiv - Cell Biology 2023Quote: Differentiated N2a cells were transfected with mRFP or syn-p2a-mRFP (1 μg) and mito GcaMP6f (1 μg) using TransIT-X2® Dynamic Delivery System (Mirus Bio) according to manufacturer’s instructions before reculturing in normal medium for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... Transit LT-1 reagent (cat. # MIR 2300) from Mirus Bio ...
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bioRxiv - Microbiology 2021Quote: ... using 3:1 TransIT-LT1 Transfection Reagent (Mirus Bio). Twenty-four hours later ...
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bioRxiv - Microbiology 2022Quote: ... by Trans IT LT-1 (Mirus Bio, Madison, WI). Single cell clones were established by the limit dilution method ...
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bioRxiv - Genomics 2023Quote: ... Transit LT-1 reagent (cat. # MIR 2300) from Mirus Bio ...
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bioRxiv - Microbiology 2022Quote: ... indicated plasmids were transfected using LT-1 Reagent (Mirus) and cells were then fixed with 5% methanol-free formaldehyde or treated with IFN or TNF-alpha before fixation ...
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bioRxiv - Molecular Biology 2019Quote: ... RRE and REV using TransIT LT-1 transfection reagent (Mirus). Viral transductions were performed in the presence of 4-8 µg/mL polybrene (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... 3 µL LT-1 transfection reagent (#MIR2020, Mirus Bio LLC), and 1 µL brain lysate (1ug/uL stock determined by BCA (5000001 ...
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bioRxiv - Microbiology 2024Quote: ... pTM-P and 1 pTM-L using TransIT-LT1 (Mirus) and incubated at 37°C ...