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Citations for Mirus Bio :
1 - 50 of 156 citations for Recombinant Human Interleukin 1 Beta since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... XGal staining was performed using the beta-galactosidase staining kit (Mirus #MIR 2600), following the provided protocol ...
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bioRxiv - Microbiology 2022Quote: Recombinant HIV-1 variants were prepared by transfection of the pNL4.3 mutants into HEK293T cells using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... recombinant replication-incompetent lentiviruses were produced by co-transfecting HEK293T cells using transIT-LT1 (Mirus) with pMD2.G and psPAX2 helper plasmids together with lentiCas9-Blast (Addgene ...
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bioRxiv - Immunology 2022Quote: ... Positive recombinant plasmid was transfected into Sf21 cells using the Flashbac Ultra Baculovirus Expression System (Mirus Bio). Viral Titers were determined with the BacPak qpcr Titration kit (Takara Bio ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculovirus encoding POLA1 (or its truncation mutant) were produced using the flashBAC ultra system (Mirus Bio). The baculoviruses encoding PRIM1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus). Viruses were then used to infect suspension High Five cells grown in Express5 media and supernatants harvested ∼60–72 hours post-infection ...
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bioRxiv - Immunology 2021Quote: ... The obtained recombinant vector was transfected into CHO-S cells using CHOgro High Yield Expression System (Mirus Bio, USA). The culture supernatant containing the RBD protein was harvested after 10 days ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Baculoviruses were produced by transfecting Sf9 cells with recombinant bacmids using TransIT®-Insect Transfection Reagent (Mirus, cat no. MIR 6100), grown for 7 days at 28ºC ...
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bioRxiv - Immunology 2021Quote: Human Alveolus Chips were transfected with plasmid DNA using the TransIT-X2 reagent (Mirus Bio). 3 ug of plasmid DNA and 6 ul TransIT-X2 reagent were constituted in 300 ul OPT-MEM before added to 3 ml chip flow medium ...
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bioRxiv - Biochemistry 2021Quote: Human POLA1 (NP_058633.2) and POLA1Δ1-337 was cloned into the pOET1 transfer vector (Mirus Bio). Human PRIM1 (NP_000937.1) ...
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bioRxiv - Physiology 2023Quote: We cloned the coding sequences of human TSHB and CGA into pLIVE vectors (Mirus Bio, Madison, WI) using a polymerase chain reaction (PCR ...
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bioRxiv - Bioengineering 2022Quote: Transfection of human pancreatic MIA PaCa-2 and BxPC-3 cells was performed with TransIT-mRNA (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... in HK-2 human proximal tubule cells at approximately 70% confluency in 96-well plates by using TransIT-2020 Reagent (Mirus, #5404), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2019Quote: ... VSV-G pseudotyped lentiviral vectors were produced by transfection of human embryonic kidney cells (HEK293FT) with third-generation lentivirus plasmids using lipofection (Mirus TransIT®-293). Supernatant was collected 48 h after transfection and concentrated using ultrafiltration (Centricon Plus-20 PLGC centrifuge filter units).
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bioRxiv - Cell Biology 2023Quote: ... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Microbiology 2021Quote: ... and RdRp-N462 or RdRp-D462 were transfected into 293T cells at a 1:1:1 ratio using TransIT LT-1 (Mirus, Madison, WI). To normalize transfection efficiency ...
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bioRxiv - Cell Biology 2024Quote: ... The human AD293 and iPSC cell lines were transfected using the Mirus TransIT®-LT1 Transfection Reagent (Cat # MIR 2300, Mirus Bio LLC, Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentivirus expressing RIPK3 shRNA was generated by transfecting HEK-293T cells in 6 well plate with a 1: 0.1: 1 ratio of pMDG2: pVSVG: pLKO.1 with TransIT-LT1 transfection reagent (Mirus). After filtering through 0.45 μm of cellulose acetate membrane (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... and TRUPATH plasmids at 1:1:1 DNA ratio (Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Biophysics 2021Quote: ... δ and ε subunits in the ratio 2:1:1:1 (TransIT® 293 transfection reagent; Mirus Bio, Madison, WI). Electrophysiological experiments started ~48 hours post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... and RSV-REV in the ratio of (3:1:1:1) into HEK293T cells using Trans-IT transfection reagent (Mirus). Virus-rich supernatant was collected at 72 hr post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µg of each sgRNA plasmid and 3 µg of dCas9-KRAB-MeCP2 plasmid were co-nucleofected into 1 × 106 mHypoA cells resuspended in a 1:1 mixture of Ingenio Electroporation reagent (Mirus Bio 50111) and OptiMEM (Gibco 31985062) ...
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bioRxiv - Microbiology 2024Quote: ... pVSV-G and either pLRGatewayIRESeFYP-Vpr/Vpx or pLRGatewayIRESmApple-Vpr/Vpx at a ratio 1:1:1 with TransIT-lenti (Mirus, MIR 6603). To generate HIV vectors to make stable cell lines ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 μl TransIT (Mirus Bio TransIT-mRNA transfection kit ...
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bioRxiv - Microbiology 2020Quote: ... or Transit LT-1 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... or Transit LT-1 (Mirus).
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bioRxiv - Microbiology 2022Quote: ... 1 µl of mRNA Boost Reagent and 1 µl of TransIT-mRNA Reagent (Mirus Bio) and incubated for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 at a ratio of 1:1:0.1 using TransIT-LT1 transfection reagent (Mirus). The resulting blend was then filtered through a cellulose acetate membrane (0.45 µm ...
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bioRxiv - Neuroscience 2023Quote: ... and pHR: mU6-sgRNA-EF1A-puro-P2A-BFP (sgRNA-BFP) (1:1:1) using 24 μL TransIT®-LT1 Transfection Reagent (Mirus Bio, Cat. No. MIR 2300) in a final volume of 1000uL Opti-MEM medium (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Bait and prey plasmids containing fused fragments were then individually transfected together with VSVG and gag/pol packaging vectors in a ratio of 3:1:1 into HEK293T cells using Trans-IT Transfection reagent (Mirus) to produce retroviruses ...
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bioRxiv - Immunology 2021Quote: ... transfected with 44 μg plasmid encoding SARS-CoV-2 Spike (pCG1-SARS-2-S, Wuhan Hu-1) using Transit LT-1 (Mirus). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using TransIT-LT-1 (Mirus) transfection reagent and 2500ng plasmid/flask ...
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bioRxiv - Cell Biology 2023Quote: ... and pMDLg/pRRE) at a 1:1 ratio and transfected into HEK293T cells using TransIT LT1 transfection reagent (Mirus Bio LLC). After 72 h virus-containing supernatants were collected ...
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bioRxiv - Cell Biology 2023Quote: Differentiated N2a cells were transfected with mRFP or syn-p2a-mRFP (1 μg) and mito GcaMP6f (1 μg) using TransIT-X2® Dynamic Delivery System (Mirus Bio) according to manufacturer’s instructions before reculturing in normal medium for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... Transit LT-1 reagent (cat. # MIR 2300) from Mirus Bio ...
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bioRxiv - Microbiology 2021Quote: ... using 3:1 TransIT-LT1 Transfection Reagent (Mirus Bio). Twenty-four hours later ...
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bioRxiv - Microbiology 2022Quote: ... by Trans IT LT-1 (Mirus Bio, Madison, WI). Single cell clones were established by the limit dilution method ...
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bioRxiv - Genomics 2023Quote: ... Transit LT-1 reagent (cat. # MIR 2300) from Mirus Bio ...
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bioRxiv - Microbiology 2022Quote: ... indicated plasmids were transfected using LT-1 Reagent (Mirus) and cells were then fixed with 5% methanol-free formaldehyde or treated with IFN or TNF-alpha before fixation ...
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bioRxiv - Molecular Biology 2019Quote: ... RRE and REV using TransIT LT-1 transfection reagent (Mirus). Viral transductions were performed in the presence of 4-8 µg/mL polybrene (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... 3 µL LT-1 transfection reagent (#MIR2020, Mirus Bio LLC), and 1 µL brain lysate (1ug/uL stock determined by BCA (5000001 ...
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bioRxiv - Microbiology 2024Quote: ... pTM-P and 1 pTM-L using TransIT-LT1 (Mirus) and incubated at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: Transfections were performed using Trans-IT LT1 (TLT-1) (Mirus Bio) according to the manufacturer’s instruction.