Labshake search
Citations for Mirus Bio :
1 - 50 of 77 citations for Recombinant Human CD22 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
-
bioRxiv - Genetics 2021Quote: Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... transfected with 9 μg HA-tagged and 9 μg Flag-tagged constructs using TransIT-LT1 transfection reagents (Mirus). The transfected cells were cultured for another two days ...
-
bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... was labeled with Cy5 (Mirus) and premixed with 5×106 CHEVs and resuspended in up to 100 µL hypo-osmolar buffer ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... or labeled with DNP (Mirus, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... and DNP-labeled RNA probe (Mirus Bio), and signals were detected using a Tyramide Signal Amplification (TSA ...
-
bioRxiv - Cancer Biology 2022Quote: ... were labeled with Cy3 using a Label IT kit (Mirus). B16F10 cells were transfected with labeled sgRNAs and Cas9 protein (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... and a Venus-tagged N-terminal β-arrestin2 using 3:1 ratio of TransiT-2020 (Mirus). 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs) ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 250 ng of plasmid expressing HA-tagged M polyprotein using TransIT-LT1 (Mirus) and after 6 h cells were treated with 1 µM Pon A or DMSO as a control ...
-
bioRxiv - Cancer Biology 2020Quote: ... Branson sonifier 450) and labeled with TM-rhodamine Label-IT (Mirus). Labeled BAC probes were suspended in commercial chromosome paint probe for chromosome 11 (Metasystems) ...
-
bioRxiv - Biophysics 2021Quote: ... RNA was Cy3 labeled using a Label IT kit (Mirus Bio), and RNA purity was verified using a 0.8% agarose gel ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 7.7 µg of a plasmid expressing HA-tagged M polyprotein using TransIT-LT1 (Mirus) and after 6 h the cells were treated with either DMSO or 1 µM ponasterone A (Pon A) ...
-
bioRxiv - Molecular Biology 2022Quote: ... samples were transfected with 5 μg of the indicated FLAG-tagged TTP wild-type or mutant expression plasmids using TransIT 293 reagent according to the manufacturer’s protocol (Mirus). 48 hours after transfection ...
-
bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with the N-terminal FLAG-tagged CDC42 expression vector using TransIT 293 (Mirus Bio LLC) in 6-well culture plates ...
-
bioRxiv - Cell Biology 2023Quote: ... and the plasmid was transfected into the cells expressing tagged AP1G1 using a TransIT-HeLaMONSTER kit (Mirus Bio LLC), according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with FAM-labeled miR-214-3p using TransIT-X2 (Mirus). Live-cell images of mCherry-PrPC (red)-FAM-labeled miR-214-3p (green ...
-
bioRxiv - Genetics 2021Quote: ... Transfection of 6xMyc-tagged RBPJ constructs (pCS2-RBPJ-6xMyc, WT and AOS variants) was carried out with TransIT-293 transfection reagent (Mirus) following the manufacture’s instruction ...
-
bioRxiv - Microbiology 2021Quote: ... 1 x 106 Jurkat cells expressing Strep-tagged ADAP1 or GFP were resuspended in 0.1 mL Mirusbio ingenio (Mirus, MIR50115) solution containing 2 μg of TF-responsive reporters driving Firefly luciferase (pGL3-3xAP1-luciferase (Addgene ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were plated in 6-well dishes and transfected with mEGFP-tagged DNAJC17 or deletion mutants using TransIt-LT1 reagent at a 3:1 ratio (Mirus). Cells were then trypsinized and replated on 4-well chambered coverglass (LabTek ...
-
bioRxiv - Immunology 2020Quote: ... recombinant replication-incompetent lentiviruses were produced by co-transfecting HEK293T cells using transIT-LT1 (Mirus) with pMD2.G and psPAX2 helper plasmids together with lentiCas9-Blast (Addgene ...
-
bioRxiv - Plant Biology 2022Quote: ... the purified miRNAs were labeled with Cy5 fluorophore (Cy5 Labeling Kit; Mirus Bio LLC) and then subjected to hybridization with the customized microarray for 4 hours in a 42°C oven ...
-
bioRxiv - Cell Biology 2020Quote: ... GFP-tagged E-cadherin or with constructs encoding E-cadherin and TNFα RUSH system using TransIT-LT1 Reagent (Mirus, MIR 2305) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... and 5 µg of the transfer plasmid (pLV-TetO-hNGN2-eGFP-puro, FudeltaGW-rtTA, myc-tagged SYNJ2BP WT, S21A or S21E) using TransIT-Lenti reagent (Mirus Bio). After 48 h (day 2) ...
-
bioRxiv - Cell Biology 2022Quote: HeLa cells were transiently transfected with pLVX-EF1alpha-nCoV2019 2xStrep- tagged SARS-CoV2 ORF constructs [47] using TransIT-HeLa Monster (Mirus Bio). After 48 hours transfection ...
-
bioRxiv - Immunology 2022Quote: ... Positive recombinant plasmid was transfected into Sf21 cells using the Flashbac Ultra Baculovirus Expression System (Mirus Bio). Viral Titers were determined with the BacPak qpcr Titration kit (Takara Bio ...
-
bioRxiv - Biochemistry 2021Quote: Recombinant baculovirus encoding POLA1 (or its truncation mutant) were produced using the flashBAC ultra system (Mirus Bio). The baculoviruses encoding PRIM1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... PolyA-RNA was covalently labeled with Cy3 using a LabelT-Cy3 kit (Mirus Bio MIR 3625) following the manufacturer instructions.
-
bioRxiv - Immunology 2020Quote: ... self-RNA was labeled using a Label IT® Nucleic Acid Labeling Kit (Mirus cat. MIR7125), and Spermidine was labeled with 2-(methylamino ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 µg pre-miR-181a-1 were labeled with cy3 using Nucleic Acid Labeling Kit (Mirus) following the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus). Viruses were then used to infect suspension High Five cells grown in Express5 media and supernatants harvested ∼60–72 hours post-infection ...
-
bioRxiv - Biochemistry 2024Quote: ... they were transfected with 1800 ng of plasmid containing either N- or C-terminal tagged GFP-AMPKγ3 using TransITx2 transfection reagent (Mirus Bio, cat no. MIR 6000) and then lysed 48 hours later (lysis buffer – 50 mM Tris ...
-
bioRxiv - Molecular Biology 2022Quote: HDRT and xHDRT DNA were Cy5-labeled using the Label IT® Nucleic Acid Labeling Reagents (Mirus) and used in a standard nucleofection protocol (see Cas9 ...
-
bioRxiv - Immunology 2021Quote: ... The obtained recombinant vector was transfected into CHO-S cells using CHOgro High Yield Expression System (Mirus Bio, USA). The culture supernatant containing the RBD protein was harvested after 10 days ...
-
bioRxiv - Microbiology 2021Quote: A ~7 kb PCR product was fluorescently labeled as described previously11 using the Cy3 LabelIT kit (Mirus Biosciences) as per manufacturer recommendations ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µg of PCR product was fluorescently labeled using the Label IT-Cy5 Nucleic Acid Labeling Kit (Mirus) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transiently transfected with 3 µg pE-hABCA3-eGFP plasmid (hABCA3-eGFP expression controlled by CMV promoter) in Trans-IT LT1 transfection reagent (Mirus). 48 h post-transfection the medium was removed ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Baculoviruses were produced by transfecting Sf9 cells with recombinant bacmids using TransIT®-Insect Transfection Reagent (Mirus, cat no. MIR 6100), grown for 7 days at 28ºC ...
-
bioRxiv - Microbiology 2022Quote: Recombinant HIV-1 variants were prepared by transfection of the pNL4.3 mutants into HEK293T cells using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... scDNAs were Cy5-labeled using the Label IT® Nucleic Acid Labeling Kit (Mirus Bio LLC, Madison, WI, USA; WI 53719), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cy5-labeled probes were generated from RP11-440N18 BAC DNA sonicated to 150 bp and labeled using a DNA labeling kit (Mirus Bio). Slides were denatured with 0.5 M NaOH for 30 min at room temperature ...
-
bioRxiv - Immunology 2019Quote: 100 ug of pCMV6 plasmid was labeled with 55 ul of Cy5 Label-IT kit for 1 hour at 37°C (Mirus Bio, Madison, WI). The labeled plasmid was purified by ethanol precipitation ...
-
bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: Human Alveolus Chips were transfected with plasmid DNA using the TransIT-X2 reagent (Mirus Bio). 3 ug of plasmid DNA and 6 ul TransIT-X2 reagent were constituted in 300 ul OPT-MEM before added to 3 ml chip flow medium ...
-
bioRxiv - Biochemistry 2021Quote: Human POLA1 (NP_058633.2) and POLA1Δ1-337 was cloned into the pOET1 transfer vector (Mirus Bio). Human PRIM1 (NP_000937.1) ...
-
bioRxiv - Immunology 2022Quote: ... plasmid was fluorescently labeled with the Cy5 fluorophores using the Mirus Label IT® tracker intracellular nucleic acid localization kit (MIR 7021, Mirus Bio, Madison, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2022Quote: ... The ctDNA was fluorescently labeled with either Cx-rhodamine or CY5 using the Label IT® Nucleic Acid Labeling kit (Mirus Bio LLC, WI).
-
bioRxiv - Molecular Biology 2023Quote: The Cas9-sgRNA and Cas12a-crRNA were labeled with CX-rhodamine (Label IT® Nucleic Acid Labeling Reagents Rhodamine Kit, Mirus Bio, Madison, WI) (75 ...