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Citations for Mirus Bio :
101 - 150 of 166 citations for PD 1 Human 147a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: A549 ZAP−/−/− and DF-1 cells were cultured in 10 cm dish and transfected using LT1 reagent (Mirus lab) with 5 μg of huZAP-GFP DNA for 24 h ...
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bioRxiv - Neuroscience 2020Quote: ... and mRFP + Nuak 1 and 2 kinases was performed via chemical transfection using TransIT-X2 (Mirus Bio, Madison, WI) 48 hours following plating ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 Mϕ were transfected with a transfection complex (50 μl) of 0.3% TransIT™-LT1 (Mirus Bio™) transfection reagent (vehicle ...
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bioRxiv - Cell Biology 2022Quote: ... Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus), and Opti-MEM Reduced Serum Medium (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... cultured in Schneider’s Drosophila Medium containing 10% heat-inactivated FBS and 1X penicillin-streptomycin with the TransIT-LT 1 reagent (Mirus). Flag-CG30403 only (no bait ...
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bioRxiv - Cell Biology 2020Quote: ... adherent Sf21 cells were transfected with plasmids expressing ARIF-1 using TransIT-Insect transfection reagent (Mirus Bio, Madison, WI). At 3 d post-transfection ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep) were transfected with 25 μg of plasmid encoding for the corresponding S glycoprotein using TransIT-Lenti (Mirus) as transfectant reagent ...
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bioRxiv - Biophysics 2022Quote: ... and 100 ug ml-1 Streptomycin were passed to 10 cm dishes and co-transfected using TransIT (Mirus Bio) in an approximate 1:2.5 ratio with NTSR1 containing C-terminal Renilla luciferase (RLuc ...
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bioRxiv - Cancer Biology 2023Quote: ... Control and BCAT1-KO U251 cells were reverse-transfected with 1µg/ml of TC3-R9P-3NLS pDNA using 1:2 of Trans-iT (Mirus). 48h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected in duplicate into HEK293T cells in a 48-well plate using LT-1 transfection reagent (Mirus) with the indicated plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... pTRIPz or pLKO.1 plasmid was co-transfected with lentiviral Packaging vectors (pMD2.G and psPAX2) into HEK293T using TransIT-LT1 (Mirus) transfection reagent following the manufacturer instruction ...
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bioRxiv - Genomics 2021Quote: ... and pMD2.G at a ratio of 9:8:1 by mass using TransIT-LT1 transfection reagent (Mirus MIR 2306) at a ratio of 3 μL transfection reagent per 1 μg plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... lentiviral particles were generated by transient transfection of the lentiviral packaging Lenti-X 293T cells (Takahara) with the TCR encoding plasmids and packaging plasmids (VSVg and PSPAX2) 25 using Transit LT-1 (Mirus). Lentiviral supernatant was harvested 2 days later ...
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bioRxiv - Biophysics 2020Quote: Plasmids for expression of WT or mutant rat KIF1A(339)-LZ motors tagged with monomeric NeonGreen and an FRB domain were cotransfected into COS-7 cells with a plasmid for expression of PEX3-mRFP-FKBP at a ratio of 6:1 with TransITLT1 transfection reagent (Mirus). Eight hours after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral particles were produced by co-transfection of shRNA plasmids with psPAX and pMDG.2 in 293T cells using the Trans-IT LT-1 transfection reagent (Mirus). DM cells were infected by shRNA lentivirus twice via spinoculation in the presence of 5 μg/mL polybrene (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... 1 x 106 Jurkat cells expressing Strep-tagged ADAP1 or GFP were resuspended in 0.1 mL Mirusbio ingenio (Mirus, MIR50115) solution containing 2 μg of TF-responsive reporters driving Firefly luciferase (pGL3-3xAP1-luciferase (Addgene ...
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bioRxiv - Immunology 2020Quote: ... pMigR1-IRES-Thy1.1 or pMigR1-Cre-IRES-Thy1.1 plasmid (Feng et al., 2015) and pCl-Eco were cotransfected into these cells with TransIT-293 Transfection Reagent (Mirus); 2-3 days after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral vectors were co-transfected with packaging vectors VSVG and gag/pol in a ratio of 3:1:2 into HEK293T cells using Trans-IT Transfection reagent (Mirus). Viral supernatants were collected at 48 and 72 hr post-transfection ...
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bioRxiv - Cell Biology 2022Quote: Plasmids for expression of kinesin-1 or any of the kinesin-6 family motors tagged with monomeric NeonGreen and an FRB domain were cotransfected into COS-7 cells with a plasmid for expression of PEX3-mRFP-FKBP or GMAP210p-mRFP-2xFKBP at a ratio of 5:1 with TransIT-LT1 transfection reagent (Mirus). Eighteen hours after transfection ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 6 well plates (2.0×105 per well) and incubated overnight before transfection with pCAGGS-VP40 using lipofectamin LT-1 (MIR2304; Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... expression in the indicated cell lines was induced with 1 μg/ml doxycycline and cells were transfected with a pcDNA3-SCAPER-FLAG construct using TransIT293 (Mirus) in 10 cm dishes ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1.5 µg of a pLKO.1-Hygro or lentiCas9-Blast plasmid using the TransIT-LT1 Transfection Reagent (Mirus Bio) as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The dCas9-KRAB-mCherry plasmid was mixed with the pCMV_ΔR8.91 and pMD2.G packaging vectors at a ratio of 9:8:1 with TransIT®-Lenti Transfection Reagent (Mirus) in optiMEM (Gibco ...
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Nucleoprotein reassortment enhanced transmissibility of H3 1990.4.a clade influenza A virus in swinebioRxiv - Microbiology 2023Quote: ... 1 µg of each plasmid was mixed with 18 µl of TransIT-LT1 transfection reagent (Mirus Bio LLC, Madison, WI). The mixture was incubated for 45 min and then used to overlay the 293T/MDCK cells overnight ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 6-well dishes and transfected with mEGFP-tagged DNAJC17 or deletion mutants using TransIt-LT1 reagent at a 3:1 ratio (Mirus). Cells were then trypsinized and replated on 4-well chambered coverglass (LabTek ...
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bioRxiv - Microbiology 2023Quote: ... Medium was changed 24 h later to 10 ml of 5 % FBS/DMEM and changed again 48 h later to 10 ml of 5 % FBS/DMEM containing 1 µg/ml of puromycin (Mirus). Cells were incubated in selection medium for 72 h ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 ΔENV pseudotyped with VSV-G were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). For HIV-1 ΔENV ΔVpr with Vpr WT packaged in trans (HIV-1ΔVpr +Vpr WT) ...
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bioRxiv - Cancer Biology 2021Quote: Retrovirus MSCV and MSCV-NKX2-1 and were produced by transfection of HEK293T cells with TransIT-293 (Mirus Bio, Madison, WI) with the retrovirus backbone as well as packaging vectors gag/pol and CMV-VSV-G ...
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bioRxiv - Bioengineering 2021Quote: ... 1×105 MC38 cells were transfected with gRNA-ligated eSpCas9 plasmids for 48 hours using TransIT-LT1 transfection reagent (Mirus Bio) in Opti-MEM (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... were transfected at a 4:3:1 ratio using TransIT293 reagent into T225 flasks as indicated by the manufacturer (Mirus Bio). For individual sgRNA preparations ...
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bioRxiv - Molecular Biology 2020Quote: ... to be integrated into the genomic FRT site was cloned into the pcDNA5/FRT/TO vector backbone and transfected together with pOG44 Flp-In recombinase in a 9:1 ratio using either Trans-IT 293 transfection reagent (Mirus, USA) or Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Lentivirus transfer vectors were packaged by co-transfection with psPAX2 and pMD2.G (4:3:1) using TransIT-2020 (Mirus Bio) in HEK293T cells ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected overnight with either a control plasmid (PLX) or TMPRSS2 (PLX-TMPRSS2) via TransIT LT-1 transfection reagent (Mirus Bio) in 100 µL of OptiMEM per well ...
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bioRxiv - Immunology 2022Quote: Monolayers of BSC-40 cells were infected with parental IHD-J at an MOI of 0.01 PFU/cell and transfected 1 hour later with 10 μg of DNA of plasmid pCyA-ISG15GG (pCyA was kindly provided by Mariano Esteban) using LT-1 (Mirus Bio) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... a 10 cm dish of cells was transfected with 9 µg of Flp-Recombinase (plasmid pOG44) and 1 µg of a specific pcDNA5/FRT plasmid using TransIT-293 transfection reagent (Mirus, MIR2705). 48 hours after transfection ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was co-transfected into S2 insect cells with 10:1 molar excess of pCoBlast using TransIT-Insect transfection reagent (Mirus Bio). Stable cell lines were selected with 100 µg/mL blasticidin (Cambridge Bioscience) ...
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bioRxiv - Immunology 2021Quote: ... a gag-pol packaging construct (0.65 μg/well) and the relevant vector construct (1 μg/well) were transfected using the TransIT®-LT1 Transfection Reagent (MIR 2306, Mirus). Two days after transfection the soups containing the viruses were collected and filtered.
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bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
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bioRxiv - Microbiology 2021Quote: ... with Benin 97/1 (Figure 1A) and then transfection with pΔEP402R-VP72GUS using the TransIT-LT1 transfection reagent (Mirus Bio, USA). In the presence of X-Gluc ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 μg pVSVG per 1 reaction in a 6-well plate well) into HEK 293T cells with TransIT-LT1 transfection reagent (Mirus #2304) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... MRC-5 cell monolayers were mock-transfected or transfected with sHCoV genomic RNA (1 μg/ml) using TransIT-mRNA Transfection Kit (Mirus Bio) at 33°C ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors were produced in HEK-293T cells by co-transfection of the previously sequenced vector constructs with psPAX and pMDG.2 packaging plasmids using Trans-IT LT-1 (Mirus, MIR2700). HEK293T cells were incubated overnight at 37°C and the next day medium was changed for IMDM 10% HI-FBS ...
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bioRxiv - Microbiology 2023Quote: RBP-overexpressing Huh7 cells were generated by transfection of RBP expression plasmids using TransIT LT-1 transfection reagent (Mirus, WI, USA). Briefly ...
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bioRxiv - Immunology 2021Quote: ... and replication-competent HIV-1 were produced by transfection into 293T cells with the TransIT-Lenti transfection reagent (Mirus Bio, Madison, WI) at a ratio of 1:3 (DNA ...
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bioRxiv - Microbiology 2021Quote: ... and a full-length plasmid containing the chimeric LLOV genome (1 μg) using TransIT®-LT1 per manufacturer’s recommendations (Mirus Bio LLC). Media was changed approximately 18 hours post-transfection and cells were monitored for cytopathic effect (CPE ...
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bioRxiv - Molecular Biology 2021Quote: ... or control siRNAs (siGENOME Non-Targeting siRNA Pool #1, D-001206-13-20) using the TransIT-X2 Dynamic Delivery System (Mirus Bio, MIR6003) for 72 h.
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 1 µg of DDX60 wt or mutant constructs using TransIT®-LT1 Transfection Reagent (Mirus Bio MIR 2300) at a 1:3 (µg to µl ...
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bioRxiv - Microbiology 2020Quote: ... the cells were transfected with 1 μg of in vitro-transcribed J6/JFH-1 Rluc RNA or H77S.3/GLuc RNA using the TransIT mRNA transfection kit (Mirus Bio LLC) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 250 ng lentiviral vector was co-transfected with a mix of third-generation lentivirus packaging vectors (250 ng total) using Mirus LT-1 transfection reagent (Mirus Bio LLC). Three days post-transfection ...
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bioRxiv - Genomics 2019Quote: ... were used to transfect 1 × 106 Huh-7 cells using the TransIT® mRNA transfection kit according to the manufacturer’s instructions (Mirus Bio LLC). At 72 h post transfection (p.t.) ...