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Citations for Mirus Bio :
1 - 50 of 159 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: or from Sigma for universal negative control #1 (SIC001) and were transfected using TransIT-X2 (Mirus) in Opti-MEM media following manufacturer’s recommendation ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected overnight with either a control plasmid (PLX) or TMPRSS2 (PLX-TMPRSS2) via TransIT LT-1 transfection reagent (Mirus Bio) in 100 µL of OptiMEM per well ...
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bioRxiv - Molecular Biology 2021Quote: ... or control siRNAs (siGENOME Non-Targeting siRNA Pool #1, D-001206-13-20) using the TransIT-X2 Dynamic Delivery System (Mirus Bio, MIR6003) for 72 h.
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bioRxiv - Immunology 2021Quote: ... FLAG-p46ATIL or an EV control using TransIT-X2 (Mirus Bio). At 24h post transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... and varying concentrations of inhibitor (or control) oligos using TransIT-LT1 (Mirus Bio) as the transfection reagent ...
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bioRxiv - Immunology 2021Quote: ... and scrambled control RNA were transfected into each well using the Transit X2 delivery system (MIRUS; MIR6003) in OptiMEM (Gibco 31985-070) ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected with OAS1 gRNA or a Cas9-expressing control vector using TransIT-X2 (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Candidate and control vectors were tested for activity by transient transfection with Trans-IT LT1 transfection reagent (Mirus) into murine neuron-like N2A cells followed by Trizol RNA isolation (Thermo ...
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bioRxiv - Genomics 2019Quote: ... HEK293 cells were transfected with either 2.5 or 8 ug of the pCMV-Aire-Flag plasmid (or control plasmid) using 7.5 or 32 ul of the TransIT-293 transfection reagent (Mirus). After 48h ...
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bioRxiv - Cancer Biology 2020Quote: ... APEH (cat# L-005785-00-0010) and control (cat# D-001810-10-05) using TransITX2 (Mirus Bio; cat#MIR6000). UCHL1 knockdown was also carried out by transducing HGSOC cells with control or UCHL1 shRNA lentiviral virus particles (Santacruz Biotechnology ...
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bioRxiv - Molecular Biology 2019Quote: ... plus pMK1221-control or −GR shRNA (2.88 ug shCtrl or shGR, respectively) using TransIT-293 Transfection Reagent (Mirus Bio) as instructed by the manufacturer ...
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bioRxiv - Physiology 2022Quote: ... cells were transfected with 25mM EP300 siRNA or scramble control siRNA via the TransIT-X2 Dynamic Delivery System (Mirus Bio), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were transfected with 40 nM siRNA targeting IFT88 or with a scrambled sequence (negative control siRNA) for 72h using TransIT-X2 Dynamic (Mirus) transfection kit ...
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bioRxiv - Immunology 2019Quote: ... and pZIP-mCMV-ZsGreen-shRNA NT Control or mTOR plasmids (2 µg; Transomic) using the TransIT-LT1 transfection reagent (Mirus) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and the gRNA-expressing plasmid (pC016-gPsp-Control or pC016-gPsp-EGFP start codon, 12 μg) using TransIT-293 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... TK-Renilla luciferase reporter plasmid (as an internal control) and the desired expression vectors or empty vector (EV) using TransIT-LT1 transfection reagent (Mirus Bio), according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: Mt1-/-Mt2-/- BMDMϕ were transfected with pCMV6-Ac-GFP control vector or pCMV6-Ac-MT3-GFP vector using the LT1 transfection reagent (Mirus Bio) in RPMI media containing 10% serum without antibiotics as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... a plasmid containing a cAMP response element binding site driving firefly luciferase was transfected with a control plasmid containing a CMV promotor driving renilla luciferase expression using the TransiT-X2 transfection reagent (Mirus Bio) 48 hours after the siRNA transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... we co-transfected the cells with 50 ng of pMIR-REPORT constructs containing the 3’UTR sequences together with 50 ng of a Renilla housekeeping control plasmid and 100 nM of miR-424 or miR-503 mimics or a miRNA negative control (Dharmacon #C-300717-05, #C-300841-05, or #CN-001000-01-05, respectively) using the TransIT-X2 reagent (Mirus 6003). 24 hours after transfection ...
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bioRxiv - Bioengineering 2022Quote: Cells cultured in a 24- or 96-well plate were transiently transfected with STF luciferase reporter and the internal control pRL-SV40 Renilla luciferase expression plasmid using TransIT-2020 (Mirus Bio), with or without Wnt1- or Wnt3a-expression construct as indicated in Results ...
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bioRxiv - Neuroscience 2022Quote: ... and Fc (a negative control) were generated using HEK293T cells transfected with the corresponding expression vectors using TransIT-PRO Transfection Reagent (Cat# MIR5740, Mirus Bio) and maintained in serum-free AIM V synthetic medium (Cat# 12055083 ...
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bioRxiv - Microbiology 2023Quote: ... HeLa cells were co-transfected on a six-well plate with a 3:1 mass ratio mix of pM1-2C and pArf1-EGFP plasmids (or a plasmid expressing EGFP instead of Arf-EGFP in controls) using Mirus 2020 transfection reagent (Mirus Bio). Infected cells were processed at 6 ...
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bioRxiv - Biochemistry 2024Quote: ... Two samples each were treated with Opti-MEM with 2 µg of transfection DNA (either pJG01, or negative control without DNA) along with TransIT-X2 transfection reagent (Mirus Bio) premixed and allowed to incubate 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 5 μM siRNA targeting FAK (Horizon Discovery, L-003164-00-0005) or control siRNA (Horizon Discovery, D-001206-13-20) using TransIT-X2 Reagent (Mirus Bio, MIR6003) in 10-cm dishes and incubated for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Microbiology 2021Quote: ... and RdRp-N462 or RdRp-D462 were transfected into 293T cells at a 1:1:1 ratio using TransIT LT-1 (Mirus, Madison, WI). To normalize transfection efficiency ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentivirus expressing RIPK3 shRNA was generated by transfecting HEK-293T cells in 6 well plate with a 1: 0.1: 1 ratio of pMDG2: pVSVG: pLKO.1 with TransIT-LT1 transfection reagent (Mirus). After filtering through 0.45 μm of cellulose acetate membrane (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... and TRUPATH plasmids at 1:1:1 DNA ratio (Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Biophysics 2021Quote: ... δ and ε subunits in the ratio 2:1:1:1 (TransIT® 293 transfection reagent; Mirus Bio, Madison, WI). Electrophysiological experiments started ~48 hours post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... and RSV-REV in the ratio of (3:1:1:1) into HEK293T cells using Trans-IT transfection reagent (Mirus). Virus-rich supernatant was collected at 72 hr post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µg of each sgRNA plasmid and 3 µg of dCas9-KRAB-MeCP2 plasmid were co-nucleofected into 1 × 106 mHypoA cells resuspended in a 1:1 mixture of Ingenio Electroporation reagent (Mirus Bio 50111) and OptiMEM (Gibco 31985062) ...
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bioRxiv - Microbiology 2024Quote: ... pVSV-G and either pLRGatewayIRESeFYP-Vpr/Vpx or pLRGatewayIRESmApple-Vpr/Vpx at a ratio 1:1:1 with TransIT-lenti (Mirus, MIR 6603). To generate HIV vectors to make stable cell lines ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 μl TransIT (Mirus Bio TransIT-mRNA transfection kit ...
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bioRxiv - Microbiology 2020Quote: ... or Transit LT-1 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... or Transit LT-1 (Mirus).
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bioRxiv - Microbiology 2022Quote: ... 1 µl of mRNA Boost Reagent and 1 µl of TransIT-mRNA Reagent (Mirus Bio) and incubated for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 at a ratio of 1:1:0.1 using TransIT-LT1 transfection reagent (Mirus). The resulting blend was then filtered through a cellulose acetate membrane (0.45 µm ...
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bioRxiv - Neuroscience 2023Quote: ... and pHR: mU6-sgRNA-EF1A-puro-P2A-BFP (sgRNA-BFP) (1:1:1) using 24 μL TransIT®-LT1 Transfection Reagent (Mirus Bio, Cat. No. MIR 2300) in a final volume of 1000uL Opti-MEM medium (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Bait and prey plasmids containing fused fragments were then individually transfected together with VSVG and gag/pol packaging vectors in a ratio of 3:1:1 into HEK293T cells using Trans-IT Transfection reagent (Mirus) to produce retroviruses ...
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bioRxiv - Immunology 2021Quote: ... transfected with 44 μg plasmid encoding SARS-CoV-2 Spike (pCG1-SARS-2-S, Wuhan Hu-1) using Transit LT-1 (Mirus). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using TransIT-LT-1 (Mirus) transfection reagent and 2500ng plasmid/flask ...
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bioRxiv - Cell Biology 2023Quote: ... and pMDLg/pRRE) at a 1:1 ratio and transfected into HEK293T cells using TransIT LT1 transfection reagent (Mirus Bio LLC). After 72 h virus-containing supernatants were collected ...
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bioRxiv - Cell Biology 2023Quote: Differentiated N2a cells were transfected with mRFP or syn-p2a-mRFP (1 μg) and mito GcaMP6f (1 μg) using TransIT-X2® Dynamic Delivery System (Mirus Bio) according to manufacturer’s instructions before reculturing in normal medium for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... Transit LT-1 reagent (cat. # MIR 2300) from Mirus Bio ...
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bioRxiv - Microbiology 2021Quote: ... using 3:1 TransIT-LT1 Transfection Reagent (Mirus Bio). Twenty-four hours later ...
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bioRxiv - Microbiology 2022Quote: ... by Trans IT LT-1 (Mirus Bio, Madison, WI). Single cell clones were established by the limit dilution method ...
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bioRxiv - Genomics 2023Quote: ... Transit LT-1 reagent (cat. # MIR 2300) from Mirus Bio ...