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Citations for Mirus Bio :
151 - 200 of 315 citations for Mouse GRK4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 1μg each of the eight plasmids were mixed well with 16 μL of TransIT-LT1 (Mirus Bio) and transfected into the hMDCK-HEK293 cocultured cells at 70% confluence ...
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bioRxiv - Genetics 2023Quote: ... Transfections of wild-type and Gly1170Ser COL2A1-encoding plasmids were performed using Transit-2020 transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... and 15 ng each of two sgRNA expression plasmids mixed with 0.3 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... pSPAX2 and Nb80-GFP and SNAP-β1AR plasmids using as transfection reagent TransIT-Lenti (mir6600, Mirus bio). Lentivirus was produced in DMEM containing 10% FBS and 1% BSA and then concentrated using the Lenti-X concentrator (Takera Bio).
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bioRxiv - Cancer Biology 2024Quote: ... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... HepG2-NTCP cells at 80-90 % confluency were transfected with the pMC-HBV plasmids using TransIT-2020 (Mirus) according to the manufacturer’s protocol in DMEM supplemented with 5 % FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... MmAtxn10::EGFP-N1 plasmids were transfected into cells using TransIT®-2020 DNA per manufacturer guidelines (Mirus, MIR5404).
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bioRxiv - Immunology 2019Quote: ... 20ug of pT3-19BBz and 1ug SB100x plasmids into a 0.2 cm cuvette (Mirus Biotech®, Madison, WI) and cells were electroporated with the U-14 program ...
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bioRxiv - Immunology 2022Quote: ... The plasmid cocktail was then gently mixed with 15 μL of TransIT LT1 transfection reagent (Mirus, GA, USA). The growth media was replaced with Opti-MEM during transfection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with the chemicals for 30 min before a transfection mixture containing 0.2 μg mCherry-expressing plasmid and 0.6 μl TransIT293 (Mirus) was added to the cells ...
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bioRxiv - Microbiology 2022Quote: ... plasmids encoding all ten reovirus genome segments were transfected into BHK-T7 cells using TransIT-LT1 (Mirus Bio). After the first signs of cytopathic effects ...
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bioRxiv - Cancer Biology 2022Quote: ... Phoenix Ecotropic (pECO) cells (ATCC CRL-3214) were transfected with plasmids using TransIT-293 transfection reagent (MIR2705, Mirus). Briefly ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected with 750 ng plasmid and selected for with 250 µg/mL hygromycin B (Mirus Bio) for 14 days ...
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bioRxiv - Cell Biology 2022Quote: ... 10g of plasmid DNA was transfected into 293T lentiviral packaging cells using LT1 transfection reagent (Mirus Bio LLC), and viral supernatants were collected ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected with 7.5 µg (total amount) of indicated plasmid(s) using TransIT-2020 transfection reagent (per the manufacturer’s recommendation, Mirus). Following 24–36 h incubation period ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 7.7 µg of a plasmid expressing HA-tagged M polyprotein using TransIT-LT1 (Mirus) and after 6 h the cells were treated with either DMSO or 1 µM ponasterone A (Pon A) ...
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bioRxiv - Molecular Biology 2023Quote: ... A transfection mix was prepared by adding 2.5 µg plasmid and 7.5 µl transfection reagent TransIT-LT1 (Mirus) in 250 µl OptiMEM medium ...
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bioRxiv - Biophysics 2023Quote: ... and 100 ng of the plasmid of interest were transiently co-transfected using 1.875 µL TransIT (Mirus Bio), and Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the barcoded viral genomes were transfected into 293T cells using TransIT-LT1 (Mirus Bio, Madison, WI), and incubated for 48 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... together with the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at a 10:7.5:5 ratio using TransIT-293 reagent (Mirus). The supernatant containing the viral particles was collected 48 hrs after transfection ...
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bioRxiv - Immunology 2021Quote: HEK 293T cells (106) were transfected in 6-well plates with corresponding plasmids with Transit LT1 transfection reagent (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
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bioRxiv - Biophysics 2022Quote: ... and 1.5 μg of the plasmid containing the gene of interest were cotransfected by using TransIT transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells (150 cm2 dish) were transfected with 6 μg of each protein-coding plasmid by using TransIT (Mirus). Cell lysis and elution were performed as described above ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids containing each of the 10 viral cDNA’s and pCAG FAST P10 plasmid were transfected into BHK-T7 cells using TransIT-LT1 (Mirus)29 ...
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bioRxiv - Microbiology 2021Quote: ... The pTM plasmids were transfected into H7-T7-IZ cells using TransIT®-LT1 Transfection Reagent (Mirus Bio LLC) following the manufacturer’s recommendations with a ratio ADN to transfection reagent of 1 to 3.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng of pcDNA3-PLPro-flipGFP-T2A-mCherry plasmid and 50 ng of protease expression plasmid pcDNA3.1-PLpro were added to each well in the presence of transfection reagent TransIT-293 (Mirus) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were transfected with 5 μg of the indicated FLAG-tagged TTP wild-type or mutant expression plasmids using TransIT 293 reagent according to the manufacturer’s protocol (Mirus). 48 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus), and Opti-MEM Reduced Serum Medium (Gibco) ...
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bioRxiv - Genomics 2020Quote: ... or with 1μg of plasmid expressing TTFI-GFP under the full CMV promoter using Transit-X2 transfection reagent (Mirus) in Opti-MEM GlutaMAX reduced-serum medium (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... adherent Sf21 cells were transfected with plasmids expressing ARIF-1 using TransIT-Insect transfection reagent (Mirus Bio, Madison, WI). At 3 d post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng pcDNA3-flipGFP-T2A-mCherry plasmid and 50 ng protease expression plasmid pcDNA3.1 were used each well in the presence of transfection reagent TransIT-293 (Mirus). 3 hrs after transfection ...
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bioRxiv - Microbiology 2021Quote: ... were seeded in 10-cm2 dishes at a density of 5e6 cells per dish and the following day transfected with 10 μg of WT or B.1.617.2 spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep) were transfected with 25 μg of plasmid encoding for the corresponding S glycoprotein using TransIT-Lenti (Mirus) as transfectant reagent ...
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bioRxiv - Zoology 2022Quote: ... Four µg of either plasmid were transfected into 6 × 105 C6/36 cells using TransIT-2020 transfection reagent (Mirus) for 4 h ...
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bioRxiv - Cell Biology 2023Quote: ... and transfected with 1.5 µg of the indicated plasmid DNA and 0.5 µg of the PiggyBac transposase construct using TransIT-X2 (MIR6004, Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of total plasmid DNA and 1.5 μL of TransIT ®-2020 Transfection Reagent (Mirus Bio, WI, USA) were mixed by gentle pipetting ...
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bioRxiv - Cell Biology 2022Quote: pQCXIH-SARS-CoV-1-ORF7a-FLAG and pQCXIH-SARS-CoV-2-ORF7a-FLAG were transfected to HEK293T cells with the packaging plasmids pMD.GagPol and pMD.VSVG using Trans-IT293 (Mirus Bio). Viral supernatants were collected 48 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: HEK293T cells were transfected with lentiviral vectors (pLVX / pLVX-TetOne) plus packaging plasmids pCMVR8.91 and pMD.VSVG using TransIT-293 (Mirus Bio). Viral supernatants were collected 48 hours after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with TCR expression vector and lentiviral transfer plasmids using TransIT-VirusGen Transfection Reagent (Mirus #MIR6700) following the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... in a 12-well plate overnight and plasmids (1.5 μg) were transfected using 3 μl of TransIT-LT1 (Mirus). For IP experiments ...
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bioRxiv - Biophysics 2023Quote: ... STARD11-GFP WT or mutant plasmids were transfected using the TransIT-X2® Transfection Reagent (Mirus Bio, MIR 6000). Cells were fixed in 3% paraformaldehyde for 20 min before staining with antibodies diluted in a PBS solution of 1% BSA and 0.05% saponin ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected in duplicate into HEK293T cells in a 48-well plate using LT-1 transfection reagent (Mirus) with the indicated plasmids ...
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bioRxiv - Microbiology 2023Quote: ... followed by transfection 6 hours post seeding using different EBOV plasmids and TransIT-LT1 transfection reagent (Mirus Bio LLC) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and gRNA plasmids for targeting PRKAA1 and PRKAA2 were co-transfected into cells with TransIT-LT1 (Mirus, MIR 2305) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and the plasmid was transfected into the cells expressing tagged AP1G1 using a TransIT-HeLaMONSTER kit (Mirus Bio LLC), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... were done by mixing 2.5 µg of plasmid DNA with 7.5 µl of TransIT-X2 reagent (Mirus, Madison, WI). The mixture was added to 105 CAF or PDAC cells cultured in serum-free and antibiotic-free media overnight.
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bioRxiv - Biochemistry 2024Quote: ... and TRUPATH plasmids at 1:1:1 DNA ratio (Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Cell Biology 2024Quote: ... the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at 10:7.5:5 ratio using TransIT-293 Transfection Reagent (MIR2704, Mirus) according to the manufacturer’s instructions ...