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Citations for Mirus Bio :
251 - 300 of 315 citations for Mouse ERP44 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were seeded in 15-cm2 dishes at a density of 10e6 cells per dish and the following day transfected with 25 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Infectious vaccine viruses were generated from plasmid constructs by transfection into BHK-21J cells using standard protocols (TransIT-LT1, Mirus Bio). The supernatant was harvested four days post-transfection when most of the cells showed signs of CPE ...
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bioRxiv - Immunology 2020Quote: ... The cells were transfected with the sgRNA transfer plasmids (MSCV-U6-sgRNA-IRES-Thy1.1) using TransIT-293 transfection reagent per the manufacturer’s protocol (Mirus, Cat# MIR 2700). The following morning ...
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bioRxiv - Immunology 2022Quote: Monolayers of BSC-40 cells were infected with parental IHD-J at an MOI of 0.01 PFU/cell and transfected 1 hour later with 10 μg of DNA of plasmid pCyA-ISG15GG (pCyA was kindly provided by Mariano Esteban) using LT-1 (Mirus Bio) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... and varying input (50 ng for 5-site screen and 15 ng for 4-site screen) of MxA plasmids were co-transfected into HEK 293T cells using the reagent TransIT-LT1 (Mirus Bio) in a 96-well plate format ...
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bioRxiv - Molecular Biology 2021Quote: psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL and psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL plasmids were transfected into cells with TransIT-293 Reagent (Mirus, MIR2704). After 24 h of incubation ...
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bioRxiv - Cell Biology 2020Quote: ... a plasmid containing a cAMP response element binding site driving firefly luciferase was transfected with a control plasmid containing a CMV promotor driving renilla luciferase expression using the TransiT-X2 transfection reagent (Mirus Bio) 48 hours after the siRNA transfection ...
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bioRxiv - Immunology 2021Quote: ... were seeded in 10-cm2 dishes at a density of 5e6 cells per dish c and the following day transfected with 10 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Maxiprep purified plasmids were transfected into HEK293FT cells using PEI or into ACH2 / U1 leukocytes via TransIT-Jurkat reagent (Mirus #2120). Supernatants were collected 72 hours after media replacement (PEI ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 μg of lentiviral plasmid were added into a microcentrifuge tube with 7.5 μL of Mirus TransIT-Lenti transfection reagent (Mirus Bio, 6604). Once all plasmids were pooled together with the transfection reagent ...
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bioRxiv - Bioengineering 2022Quote: Cells cultured in a 24- or 96-well plate were transiently transfected with STF luciferase reporter and the internal control pRL-SV40 Renilla luciferase expression plasmid using TransIT-2020 (Mirus Bio), with or without Wnt1- or Wnt3a-expression construct as indicated in Results ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral vector was produced by transforming the produced LARRY plasmid into HEK293T cells using the Trans-IT 293 transfection reagent (Mirus #MIR2700). For each of eighteen 10 cm dishes of HEK293T cells ...
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bioRxiv - Microbiology 2022Quote: ... 6-well plates seeded with HEK293T cells were transfected with the m145 containing expression plasmids using TransIT-X2® dynamic delivery transfection system (Mirus) and cells were harvested at 48 hours’ post transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 µg of the transfer plasmid (pLV-TetO-hNGN2-eGFP-puro, FudeltaGW-rtTA, myc-tagged SYNJ2BP WT, S21A or S21E) using TransIT-Lenti reagent (Mirus Bio). After 48 h (day 2) ...
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bioRxiv - Genomics 2022Quote: Lentivirus was generated by transfecting HEK239T cells with the transfer plasmid and four packaging plasmids (for expression of VSV-G, Gag/Pol, Rev, and Tat) using TransIT-LT1 Transfection Reagent (Mirus Bio). Viral supernatant was harvested two days after transfection and filtered through 0.44 µm PES filters and/or frozen at –80 °C prior to transduction.
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bioRxiv - Cell Biology 2022Quote: ... cells in each well were transfected with a plasmid encoding GFP-2A-POI (protein of interest) using TransIT-2020 transfection reagent (Mirus #5404) as follows ...
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bioRxiv - Microbiology 2023Quote: ... 2 x 105 cells were seeded in 12-well plates and transiently transfected with 2 μg of a plasmid encoding HCoV-OC43 N or eGFP with TransIT-LT1 Transfection Reagent (Mirus Bio). At indicated time post transfection ...
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bioRxiv - Microbiology 2023Quote: ... were cotransfected with 1 μg psPAX2-IN/HiBiT,23 1 μg pWPI-Luc2,23 and 500 ng plasmids expressing parental S or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transfected with pCAG-Cas9-EGFP (gift from Randy Platt) and sgRNAs plasmids with TransIT-LT1 Transfection Reagent (Mirus, MIR2305) and after 48 h were sorted with BD FACSAria III Cell Sorter (BD FACSDiva Software ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral vector was co-transfected with pSPAX2 and pMD2.G plasmids (gifts from Dr. Julius lab) to HEK293T by TransIT-Lenti transfection reagent (Mirus Bio). The lentivirus was produced in DMEM containing 10% FBS and 1% BSA and then concentrated by the Lenti-X concentrator (Takara Bio).
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bioRxiv - Immunology 2023Quote: ... were seeded in 15-cm2 dishes at a density of 10 × 106 cells per dish and the following day were transfected with 25 µg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: RBP-overexpressing Huh7 cells were generated by transfection of RBP expression plasmids using TransIT LT-1 transfection reagent (Mirus, WI, USA). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors were produced in HEK-293T cells by co-transfection of the previously sequenced vector constructs with psPAX and pMDG.2 packaging plasmids using Trans-IT LT-1 (Mirus, MIR2700). HEK293T cells were incubated overnight at 37°C and the next day medium was changed for IMDM 10% HI-FBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell lines stably expressing dCas9-KRAB or dCas9-P300 were transfected with plasmids encoding gRNAs or lentiGuide empty vector using TransIT-X2 (Mirus Bio). RNA was extracted 2 days after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... the generated vectors and the PIK packaging plasmid were delivered into HEK 293T cells using the TransIT-LT1 transfection reagent (Mirus Bio). Stable MEF cell lines expressing either wild-type (WT ...
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bioRxiv - Biophysics 2024Quote: ... Lentiviral particles containing each plasmid were produced by transfecting standard packaging vectors along with the plasmids into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). Media was changed at 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1.5 μg of lentiviral plasmid were added into a microcentrifuge tube with 7.5 μL of Mirus TransIT-Lenti transfection reagent (Mirus Bio, 6604). Once all plasmids were pooled together with the transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... plasmid vectors coding the glycoproteins were transfected into BSR-T7 cells using the TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR 2300). At 16 h post-transfection ...
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bioRxiv - Genomics 2020Quote: ... cells had reached 80% confluence and were transfected with plasmids for lentivirus production using TransIT-LT1 transfection reagent (Mirus Bio, Madison, WI). 3 μl transfection reagent was added to a total volume of 15 μL Opti-MEM (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T-EGFP cells (donor cell) were transiently transfected with wild-type and mutant spike plasmids using TransIT-X2 transfection reagent (Mirus #MIR 6000). HEK293T-ACE2 and HEK293T (as a negative control ...
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bioRxiv - Cancer Biology 2019Quote: ... allowed to grow overnight and then transfected with either ShhN or mSMO plasmid DNA (6 µg DNA per 6 cm dish) using TransIT transfection reagent (Mirus Bio LLC) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the RP172 plasmids containing the CHST genes were co-transfected with RP18 and RP19 helper plasmids into HEK293T cells after incubating with TransIT®-LT1 transfection reagent (Mirus Bio). After 3 days ...
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bioRxiv - Microbiology 2021Quote: ... and a full-length plasmid containing the chimeric LLOV genome (1 μg) using TransIT®-LT1 per manufacturer’s recommendations (Mirus Bio LLC). Media was changed approximately 18 hours post-transfection and cells were monitored for cytopathic effect (CPE ...
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bioRxiv - Genetics 2020Quote: ... shUBR5 and shScrambled-pLKO.1-puro were co-transfected with pCMV-VSV-G and pCMV-dR8.2 dvpr plasmids into HEK 293T cells using TransIT293 reagent (Mirus Bio LLC, USA). To generate stable silenced shUBR5 cells ...
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bioRxiv - Genetics 2021Quote: Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were passaged to 12-well tissue culture plates at a density of 1.5×105 cells/well (3.9×104 cells/cm2) and transfected with 500 ng plasmid DNA using 2 uL of TransIT-X2 transfection reagent (Mirus Bio, #MIR6005) and 100 uL Opti-MEM I reduced serum medium (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and eIF2αS/S or eIF2αA/A MEF cells were transiently transduced with TauRD(P301S)-YFP plasmid using TransIT LT-1 Transfection reagent (#MIR2020, Mirus Bio LLC) and virally transduced by lab-prepared lentiviral infection following manufacturer’s instruction and incubated for 2 days ...
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bioRxiv - Microbiology 2022Quote: 3×105cells were seeded in 6-well plates and transfected with 3CLpro plasmids via TransIT-PRO (Mirus Bio LLC, Madison, WI, USA) and incubated overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µg of each sgRNA plasmid and 3 µg of dCas9-KRAB-MeCP2 plasmid were co-nucleofected into 1 × 106 mHypoA cells resuspended in a 1:1 mixture of Ingenio Electroporation reagent (Mirus Bio 50111) and OptiMEM (Gibco 31985062) ...
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bioRxiv - Microbiology 2023Quote: ... For CPV infections, MEF cells was transfected with feline TfR1 plasmid (pCTfR, 2.5 μg)25 by using TransIT-LT1transfection reagent (Mirus Bio, WI, USA). To generate MEFDyn1,2,3 TKO cells stably expressing hACE2 ...
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bioRxiv - Cell Biology 2023Quote: ... the packaging cell line HEK293-FT was transfected with pTCP-MPsfGFP together with pSAX2 (12260 Adgene) and pCMV-VSV-G (8454 Adgene) plasmids using the kit TransIT®-293 Transfection Reagent (Mirus Bio) and following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: HIV particles were produced by transfection of HEK293T cells with viral plasmid DNA using Mirus LT1 reagent (Mirus Bio LLC, Madison WI). At 24h ...
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bioRxiv - Microbiology 2023Quote: 3 x 105 cells were seeded per well in 6-well plates and transfected one day after seeding with 3CLpro plasmids using TransIT-PRO (Mirus Bio LLC) and incubated 8-9 hours for Mpro-On and 10 hours for Mpro-Off assays ...
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bioRxiv - Biochemistry 2023Quote: ... culture medium was replaced with 345 µL fresh DMEM per well and mammalian expression plasmids were transfected into cells using the TransIT-HeLaMONSTER Transfection Kit (Mirus Bio #MIR2904) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Flp-In 293 T-Rex cells were transfected with the different pcDNA5-FRT-TO plasmid constructs and the pOG44 vector using the TransIT2020 cationic lipid (Mirus Bio LLC), according to the manufacturer’s instructions (ThemoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg pWPI-Luc2 (a reporter plasmid encoding a firefly luciferase gene14) and 500 ng plasmids expressing parental S protein or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) or TransIT-LT1 (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 x 105 293T cells per well were seeded in 6-well plates and transfected one day later with GFP-Mpro-L plasmids using TransIT-pro (Mirus Bio LLC). After 10 hours ...
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bioRxiv - Neuroscience 2019Quote: ... VSV-G pseudotyped lentiviral vectors were produced by transfection of human embryonic kidney cells (HEK293FT) with third-generation lentivirus plasmids using lipofection (Mirus TransIT®-293). Supernatant was collected 48 h after transfection and concentrated using ultrafiltration (Centricon Plus-20 PLGC centrifuge filter units).
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with 3 µg of either each GPC expression plasmid or pCAGGS empty vector using TransIT LT-1 reagent (Mirus, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed after 1 h and cells were transfected with a mixture of plasmids using TransIT-LT1 (Mirus Bio # MIR 2300), including pVSV-eGFP-deltaG_SARS-CoV-2 Spike (5 µg) ...