Labshake search
Citations for Mirus Bio :
201 - 250 of 315 citations for Mouse ATPAF2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... pTRIPz or pLKO.1 plasmid was co-transfected with lentiviral Packaging vectors (pMD2.G and psPAX2) into HEK293T using TransIT-LT1 (Mirus) transfection reagent following the manufacturer instruction ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Viral stocks of NL43 were produced by transfection of HEK293 cells with the molecular clone plasmid using TransIT-LT1 (Mirus) transfection reagent ...
-
bioRxiv - Microbiology 2019Quote: ... HEK293 cells in 6 well plates were transfected with 500 ng/well of each protein-coding plasmid by using TransIT (Mirus). After 48 h ...
-
bioRxiv - Microbiology 2019Quote: ... NL4-3 and NL4-3(AD8) HIV viral stocks were produced by transfection of HEK293 cells with the molecular clone plasmids using TransIT-LT1 (Mirus) transfection reagent ...
-
bioRxiv - Microbiology 2019Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: ... were transfected with 2·5 µg serine protease expression plasmid or empty vector using TransIT-X2 Dynamic Delivery System (Mirus). One day later ...
-
bioRxiv - Synthetic Biology 2021Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
-
bioRxiv - Biophysics 2020Quote: Plasmids for expression of WT or mutant rat KIF1A(339)-LZ motors tagged with monomeric NeonGreen and an FRB domain were cotransfected into COS-7 cells with a plasmid for expression of PEX3-mRFP-FKBP at a ratio of 6:1 with TransITLT1 transfection reagent (Mirus). Eight hours after transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... and the packaging plasmids psPAX2 and pVsVg (all three kindly provided by Prof. Dieter Edbauer) using TransIT-Lenti transfection reagent (Mirus). The transfection mix was incubated for 20 min at room temperature (RT ...
-
Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
-
bioRxiv - Immunology 2022Quote: 30,000 Vero-TMPRSS2 cells were reverse transfected with 100 ng of pCAGGS-S plasmids in a black 96-wellplate using TransIT-LT1 Transfection reagent (Mirus) in complete growth medium (containing 10% FBS) ...
-
bioRxiv - Microbiology 2020Quote: ... the above-described PiggyBac-derived plasmid was transfected into KO Fhl1 HEK 293T cell line using TransIT-X2 reagent (Mirus). Next day ...
-
bioRxiv - Microbiology 2021Quote: ... pcDNA6-SARS-CoV-2-ORF3a-FLAG and pcDNA6-SARS-CoV-2-ORF8-FLAG plasmids (kind gifts of Prof Peihui Wang, Shandong University, China) using a commercial liposome method (TransIT-LT1, Mirus). Transfection mixtures containing plasmid DNA ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transfected using Grem1 sgRNA-containing plasmid or the cloning backbone (empty vector) using the TransIT-X2 Dynamic Delivery System (Mirus). After 24 hrs ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transiently transfected with 3 µg pE-hABCA3-eGFP plasmid (hABCA3-eGFP expression controlled by CMV promoter) in Trans-IT LT1 transfection reagent (Mirus). 48 h post-transfection the medium was removed ...
-
bioRxiv - Developmental Biology 2021Quote: ... E14 ESCs were nucleofected with 4ug linearized 2C-GFP plasmid and plated at low density in 10cm2 plates then selected with 250 μg/mLG418 (Mirus) for 8 days ...
-
bioRxiv - Cell Biology 2020Quote: ... and the corresponding cargo plasmid were transfected into 293T cells with the TransIT-LT1 transfection reagent (Mirus, Cat No. MIR230). 48 hours following transfection ...
-
bioRxiv - Immunology 2021Quote: ... transfected with 44 μg plasmid encoding SARS-CoV-2 Spike (pCG1-SARS-2-S, Wuhan Hu-1) using Transit LT-1 (Mirus). The next day ...
-
bioRxiv - Immunology 2021Quote: ... 293T-ACE2 cells seeded in 24-well plates (100,000 cells/well) were transfected with 250ng of plasmid (Empty vector, p42, p46, and p46 ATIL) using the TransIT X2 kit (Mirus). A duplicate plate was transfected at the same time in order to confirm OAS1 expression by immunoblot ...
-
bioRxiv - Immunology 2020Quote: ... pMigR1-IRES-Thy1.1 or pMigR1-Cre-IRES-Thy1.1 plasmid (Feng et al., 2015) and pCl-Eco were cotransfected into these cells with TransIT-293 Transfection Reagent (Mirus); 2-3 days after transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... Neuron progenitors were cultured on PO/LA coated plate and transfected with the RFP-LEPR carrying plasmid using the TransIT-Neural transfection reagent (Mirus) for 48 hours before leptin treatment ...
-
bioRxiv - Microbiology 2022Quote: ... a T25 flask of BHK-21 cells at 75% confluency was transfected with 10 μg of MAYV CMV-promoter driven plasmid using TransIT-LT1 Transfection Reagent (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 250 ng of DNA (split evenly by mass between the plasmids indicated) using TransIT-LT1 (Mirus). Cells were either incubated for 24 h (untreated) ...
-
bioRxiv - Immunology 2022Quote: ... were seeded in 15-cm2 dishes at a density of 10L×L106 cells per dish and the following day were transfected with 25Lµg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The mix of 47 RTU plasmids was transiently cotransfected in a suspension of assay cells using TransIT-LT1 reagent (Mirus). The transfected cells were plated into 12-well plates ...
-
bioRxiv - Cancer Biology 2022Quote: ... KB2P tumor-derived cells were transduced with the cloned lentiCRISPR v2 constructs and KB1P cells were transfected with the generated pX330.puro plasmids using a transfection reagent (TransIT-LT1 from Mirus) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 1.5 µg of a pLKO.1-Hygro or lentiCas9-Blast plasmid using the TransIT-LT1 Transfection Reagent (Mirus Bio) as per the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: HEK293T cells were transfected with retroviral packaging plasmids (pVSV-G, pBS-CMV-gagpol) and pMRX-IP-HaloTag7-LC3 using TransIT-LT1 (Mirus). The viral supernatants were collected 72 hr after transfection followed by virus concentration using Lenti-X concentrator (TaKaRa) ...
-
bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 25,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx using TransIT-X2 (MIR 6003, Mirus) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the gRNA-expressing plasmid (pC016-gPsp-Control or pC016-gPsp-EGFP start codon, 12 μg) using TransIT-293 (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... were cultured in a 100 mm plate overnight and plasmids (15 μg) were transfected using 45 μl of TransIT-LT1 (Mirus). The cells were cultured for 48 h ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sanger sequencing-verified pX330 plasmids containing the correct sequences of gRNAs were transfected into KB1P-G3 cells using the TransIT-LT1 transfection reagent (Mirus) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... The cells were transfected at a confluency of ∼80% with 600-1000 ng of plasmid and 6 uL of TransIT293T (Mirus) transfection reagent ...
-
bioRxiv - Microbiology 2023Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
-
bioRxiv - Cell Biology 2023Quote: WT cells were transfected with the sgRNA-containing plasmids by electroporation with a Nucleofector device (Lonza; in Mirus transfection solution). Cells were plated in culture dishes and maintained for three days ...
-
Nucleoprotein reassortment enhanced transmissibility of H3 1990.4.a clade influenza A virus in swinebioRxiv - Microbiology 2023Quote: ... 1 µg of each plasmid was mixed with 18 µl of TransIT-LT1 transfection reagent (Mirus Bio LLC, Madison, WI). The mixture was incubated for 45 min and then used to overlay the 293T/MDCK cells overnight ...
-
bioRxiv - Developmental Biology 2023Quote: ... was designed to include the nucleotide insertion according to published methods(Liang et al. 2017.) The iPSCs were reverse transfected with plasmid and donor oligos using TranIT-LT1 (Mirus). Green fluorescence-activated cell sorting and replated in Matrigel ...
-
bioRxiv - Immunology 2024Quote: ... Immortalized Hoil1f/f MEFs were transiently transfected with a plasmid expressing Cre-recombinase (pTrip-CMV-Cre-Puro-T2A-RFP) using TransIT-LT1 (Mirus), and Hoil1-/- and Hoil1f/f single-cell clones isolated and identified by PCR ...
-
Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... The VSV-G pseudotyped lentivirus was packaged by 5 plasmid co-transfection of HEK293T cells using TansIT Transfection Reagent (Mirus Bio) in 15 cm culture dishes (The protocol describing production of lentiviral particles can be found at http://www.www.bu.edu/dbin/stemcells/protocols) ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEK293T-GFP cells were transiently transfected with wild-type and mutant spike plasmids using TransIT-X2 transfection reagent (Mirus #MIR 6000) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... px459 CRISPR/Cas9 plasmids with or without gRNA sequence were transfected into HEK293T using TransIT-LT1 transfection reagent (Mirus, MIR 2306). Puromycin (1 μg/mL ...
-
bioRxiv - Microbiology 2020Quote: ... were seeded in 10-cm dishes at a density of 1e5 cells/cm2 and the following day transfected with 5 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... 1×105 MC38 cells were transfected with gRNA-ligated eSpCas9 plasmids for 48 hours using TransIT-LT1 transfection reagent (Mirus Bio) in Opti-MEM (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... TK-Renilla luciferase reporter plasmid (as an internal control) and the desired expression vectors or empty vector (EV) using TransIT-LT1 transfection reagent (Mirus Bio), according to the manufacturer’s instruction ...
-
bioRxiv - Immunology 2022Quote: ... and transfer plasmids were co-transfected into the packaging cell line HEK-293T (clone 17; ATCC) using TransIT-LT1 (Mirus, MIR2305). Media containing the lentivirus was collected after transfection ...
-
bioRxiv - Cancer Biology 2022Quote: ... Iso-2 and Cdk1 and its mutant plasmids were transfected into the cells using TransIT-X2 transfection reagent (Mirus, cat# MIR6000). siRNA oligonucleotides specific for Rad 51 were purchased from Sigma (ESIRNA HUMAN RAD51 EHU045521) ...
-
bioRxiv - Microbiology 2020Quote: ... cells were transfected overnight with either a control plasmid (PLX) or TMPRSS2 (PLX-TMPRSS2) via TransIT LT-1 transfection reagent (Mirus Bio) in 100 µL of OptiMEM per well ...
-
bioRxiv - Microbiology 2021Quote: ... were seeded in 10-cm dishes at a density of 1e5 cells/cm2 and the following day transfected with 5 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...