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Citations for Mirus Bio :
151 - 180 of 180 citations for EphA7 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg DNA and 8 μl TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Cell Biology 2021Quote: ... C-terminal GFP-2x Strep-tagged full length and truncation versions of CEP90 were cloned into pLVX-EF1α-IRES-Puro backbone and transfected into 293T cells using TransIT-293 transfection reagent (Mirus Bio) according to manufacturer guidelines.
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral vector was produced by transforming the produced LARRY plasmid into HEK293T cells using the Trans-IT 293 transfection reagent (Mirus #MIR2700). For each of eighteen 10 cm dishes of HEK293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... carrying the sequence of the gene to be over expressed and resistance to puromycin into 0.8×106 HEK293 cells in a 60 mm culture dish with 6 µl of TrasnIT (Cat. Nº: Mirus Bio. 293) containing 3 ml of complete media ...
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bioRxiv - Microbiology 2023Quote: ... were cotransfected with 1 μg psPAX2-IN/HiBiT,23 1 μg pWPI-Luc2,23 and 500 ng plasmids expressing parental S or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 7 and 10 in this background were obtained by transfecting the adherent parental cell line with PX459 encoding the respective sgRNA using TransIT-293 transfection reagent (Mirus, USA). 48 h post transfection ...
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bioRxiv - Neuroscience 2024Quote: ... cells in one 35 mm dish were either transiently transfected with the construct to be imaged using TransIT-293 lipofection reagent (Mirus Bio) or were virally transduced with a lentivirus ...
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bioRxiv - Immunology 2022Quote: ... and transfer plasmids were co-transfected into the packaging cell line HEK-293T (clone 17; ATCC) using TransIT-LT1 (Mirus, MIR2305). Media containing the lentivirus was collected after transfection ...
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bioRxiv - Microbiology 2019Quote: ... ZIKV was rescued by transfection of the infectious clone in semi-confluent HEK-293T cells using TransIT-LT1 transfection reagent (Mirus Bio). For both viruses ...
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bioRxiv - Microbiology 2019Quote: ... and varying input (50 ng for 5-site screen and 15 ng for 4-site screen) of MxA plasmids were co-transfected into HEK 293T cells using the reagent TransIT-LT1 (Mirus Bio) in a 96-well plate format ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 μg pVSVG per 1 reaction in a 6-well plate well) into HEK 293T cells with TransIT-LT1 transfection reagent (Mirus #2304) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the generated vectors and the PIK packaging plasmid were delivered into HEK 293T cells using the TransIT-LT1 transfection reagent (Mirus Bio). Stable MEF cell lines expressing either wild-type (WT ...
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bioRxiv - Neuroscience 2024Quote: ... and maintained in the media described above supplemented with either 1.5ug/mL (HEK-293T and HeLa) or 10µg/mL puromycin (MDCK) (Mirus Bio, MIR 5940). A clonal MDCK-ALPL line with high transcytosis activity (MDCK-ALPL clone 21 ...
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bioRxiv - Microbiology 2022Quote: Recombinant HIV-1 variants were prepared by transfection of the pNL4.3 mutants into HEK293T cells using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... the packaging cell line HEK293-FT was transfected with pTCP-MPsfGFP together with pSAX2 (12260 Adgene) and pCMV-VSV-G (8454 Adgene) plasmids using the kit TransIT®-293 Transfection Reagent (Mirus Bio) and following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg pWPI-Luc2 (a reporter plasmid encoding a firefly luciferase gene14) and 500 ng plasmids expressing parental S protein or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) or TransIT-LT1 (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... TANGO1ShortΔPRD-mCh-ILID-pHRSIN and EGFP-SspB-Sec23A-pHRSIN were separately added to 8× 105 HEK−293T cells with a packaging vector pool (comprising of pCMV 8.91 and pMDG) using TransIT®-293 transfection reagent (Mirus Bio LLC). Post transfection cells were incubated ...
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bioRxiv - Genetics 2019Quote: ... Y15F and Y15F mutant cDNA were generated as described above and transfected into HEK-293T cells (ATCC; CRL-3216) using TransIT®-LT1 Transfection Reagent (Mirus; MIR2305) following user instructions ...
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bioRxiv - Genetics 2020Quote: ... shUBR5 and shScrambled-pLKO.1-puro were co-transfected with pCMV-VSV-G and pCMV-dR8.2 dvpr plasmids into HEK 293T cells using TransIT293 reagent (Mirus Bio LLC, USA). To generate stable silenced shUBR5 cells ...
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bioRxiv - Systems Biology 2020Quote: ... 293T ACE2 cells (gift of Michael Farzan) were transduced with pTRIP-SFFV-Hygro-TMPRSS2 using TransIT®-293 Transfection Reagent (Mirus Bio, MIR 2700) to obtain 293T ACE2/TMPRSS2 cells ...
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bioRxiv - Immunology 2023Quote: All transfections were performed on HEK 293T/17 cells in the presence of serum-free DMEM and Trans-IT transfection reagent (Mirus Bio, Madison, WI, #MIR 2305). All transductions were performed by spinoculation at 1100 x g for 30 min at 30°C.
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bioRxiv - Immunology 2021Quote: Human Alveolus Chips were transfected with plasmid DNA using the TransIT-X2 reagent (Mirus Bio). 3 ug of plasmid DNA and 6 ul TransIT-X2 reagent were constituted in 300 ul OPT-MEM before added to 3 ml chip flow medium ...
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bioRxiv - Biochemistry 2021Quote: Human POLA1 (NP_058633.2) and POLA1Δ1-337 was cloned into the pOET1 transfer vector (Mirus Bio). Human PRIM1 (NP_000937.1) ...
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bioRxiv - Physiology 2023Quote: We cloned the coding sequences of human TSHB and CGA into pLIVE vectors (Mirus Bio, Madison, WI) using a polymerase chain reaction (PCR ...
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bioRxiv - Bioengineering 2022Quote: Transfection of human pancreatic MIA PaCa-2 and BxPC-3 cells was performed with TransIT-mRNA (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... in HK-2 human proximal tubule cells at approximately 70% confluency in 96-well plates by using TransIT-2020 Reagent (Mirus, #5404), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... The human AD293 and iPSC cell lines were transfected using the Mirus TransIT®-LT1 Transfection Reagent (Cat # MIR 2300, Mirus Bio LLC, Madison, USA) according to the manufacturer’s instructions ...