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Citations for Mirus Bio :
1 - 50 of 106 citations for DNA Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and standard packaging vectors using the TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). Supernatant was collected 48 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Lentivirus was made by transfecting Lx293T with standard packaging vectors and TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). Viral supernatant was harvested two days after transfection ...
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bioRxiv - Genetics 2023Quote: ... lentiviral particles were produced by transfecting standard packaging vectors into HEK293T using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). At <24 hours post-transfection culture media with exchanged with fresh media supplemented with ViralBoost (Alstem Bio ...
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bioRxiv - Cancer Biology 2020Quote: Lentivirus was generated by transfecting HEK39T cells with standard packaging vectors using TransIT®-LT1 Transfection Reagent (Mirus Bio). Viral supernatant was harvested 2-3 days after transfection and filtered through 0.44 µm PVDF filters and/or frozen prior to transduction.
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bioRxiv - Cell Biology 2021Quote: Lentivirus were generated by transfecting HEK293T cells with standard packaging vectors using TransIT-LT1 Transfection Reagent (Mirus Bio, Madison, WI) or Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: Lentivirus was generated by transfecting HEK39T cells with standard 4th generation packaging vectors using TransIT-LT1 Transfection Reagent (Mirus Bio). Media was changed 10 hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-Lenti Trasfection Reagent (6605, Mirus). CH157-MN cells were stably transduced with lentiviral particles to generate CH-157MN NOTCH3ICD or empty pLVX vector (EV ...
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bioRxiv - Cell Biology 2024Quote: ... Generation of lentivirus was performed by transfecting HEK293T cells with standard delta VPR and VSVG packaging vectors paired with TransIT-LTI Transfection Reagent (MIR2305, Mirus). Lentivirus was harvested 72 hrs following transfection and frozen at -80°C.
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced by transfecting HEK293T with standard packaging vectors using the TransIT-LT1 Transfection Reagent (MIR 2306; Mirus Bio LLC). Viral supernatant was collected at 48h and 72 h after transfection ...
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bioRxiv - Microbiology 2020Quote: Infectious vaccine viruses were generated from plasmid constructs by transfection into BHK-21J cells using standard protocols (TransIT-LT1, Mirus Bio). The supernatant was harvested four days post-transfection when most of the cells showed signs of CPE ...
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bioRxiv - Biophysics 2024Quote: ... Lentiviral particles containing each plasmid were produced by transfecting standard packaging vectors along with the plasmids into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). Media was changed at 24 hours post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transfected with sgRNA library lentivirus vectors and standard packaging vectors (VSVG and psPAX2) using TransIT-LTI transfection reagent (Mirus, Cat# 2306) following previously published protocols.17 Supernatant was harvested 72 h after transfection and filtered through a 0.45 μm SFCA filter ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-LT1 Transfection Reagent (MIR 2306; Mirus Bio LLC). Viral supernatant was collected 48–72 h after transfection ...
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bioRxiv - Microbiology 2022Quote: ... using Mirus 2020 DNA transfection reagent (Mirus). The infectious virions were harvested in the culture supernatant 48 h post-transfection ...
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bioRxiv - Bioengineering 2022Quote: ... was labelled with Label It DNA kit (Mirus), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... The plasmid DNA and transfection reagent TranSIT (Mirus) were mixed in 100 μL Opti-MEM without phenol red (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA was transfected using TransIT (Mirus Bio) by adding 1 µl of the reagent and 1 µg of DNA per 48-plate well ...
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bioRxiv - Cancer Biology 2024Quote: ... Cy5-labeled probes were generated from RP11-440N18 BAC DNA sonicated to 150 bp and labeled using a DNA labeling kit (Mirus Bio). Slides were denatured with 0.5 M NaOH for 30 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Two samples each were treated with Opti-MEM with 2 µg of transfection DNA (either pJG01, or negative control without DNA) along with TransIT-X2 transfection reagent (Mirus Bio) premixed and allowed to incubate 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... allowed to grow overnight and then transfected with either ShhN or mSMO plasmid DNA (6 µg DNA per 6 cm dish) using TransIT transfection reagent (Mirus Bio LLC) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA mixtures were mixed with TransIT-X2 (Mirus) at a ratio of 0.5 µL of TransIT-X2 per 100 ng of total DNA ...
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bioRxiv - Bioengineering 2022Quote: ... DNA plasmid was labelled with Label IT® (Mirus, USA). Reagents used for immunofluorescence are listed in Supp ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with plasmid DNA and TransIT-Insect Reagent (Mirus), followed by selection with 2 μg/ml puromycin (Life technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA were transfected into cells using TransIT-LT1 (Mirus Bio) with the 1:3 DNA-to reagent ratio ...
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bioRxiv - Biophysics 2023Quote: ... The DNA is labelled with covalent dye Mirus-488 (Mirus Bio) at a dye:basepair ratio 1:5 using the Mirus Label IT Nucleic Acid Labeling Kit (Mirus Bio).
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bioRxiv - Biophysics 2021Quote: ... 200–400 ng of plasmid DNA was transfected using Transit 293T (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Both plasmid DNA and RNA mimics were transfected using Transit-X2 (Mirus), following manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plated HEK293 cells were transfected using TransIT DNA transfection reagent (Mirus) following the instructions supplied by the manufacturer and incubated until use ...
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bioRxiv - Cancer Biology 2022Quote: ... as the DNA diluent and either TransIT-LT1 (Mirus, cat. MIR 2300), TransfeX (ATCC ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was transfected using either the TransIT-LT1 Transfection Reagent (Mirus) or Polyethylenimine (PEI ...
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bioRxiv - Microbiology 2023Quote: ... at a ratio of 3:1 PEI:total DNA or TransIT-LT1 (Mirus) at a ratio of 3:1 TransIT:DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA and siRNA transfections were performed with TransIT-LT1 (Mirus Bio) and Lipofectamine RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmid DNA and siRNA transfections were performed with TransIT-LT1 (Mirus Bio) and Lipofectamine RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... with ∼ 600ng DNA and 2 µL TransIT-LT1 transfection reagents (Mirus Bio Corporation) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and pC-L using TansIT-LT1 DNA transfection reagent (Mirus Bio, Madison, WI). The total amount of DNA transfected ranged from 0.8 μg to 2.6 μg depending on the volume of pC-NP and pC-L added ...
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bioRxiv - Immunology 2020Quote: ... The DNA was combined with 430 µL of TransIT-LT1 reagent (Mirus, MIR2305) in a mix made up to 8 mL with OptiMEM (Gibco ...
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bioRxiv - Biophysics 2023Quote: ... 2 µg of plasmid DNA was transfected per dish using Transit 293T (Mirus) following the manufacturer’s instructions and measured at least 48 h later.
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bioRxiv - Immunology 2023Quote: ... All plasmid DNA transfections were conducted using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA transfection into cells was performed with TransIT®-LT1 Transfection Reagent (Mirus Bio) by following the manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of viral genomic DNA with TransIT-293 (Mirus) and left O/N ...
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bioRxiv - Bioengineering 2023Quote: ... MCF7 cells were transfected with 2ug of DNA constructs using TransIT-LT1 reagent (Mirus), following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... DNAs were transfected into 293T cells using TransIT®-Lenti Transfection Reagent (Mirus, MIR2304). Viral supernatants were collected ...
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bioRxiv - Microbiology 2023Quote: ... Total of 2.49 μg plasmid DNA with 6.25 μL TransIT LT1 transfection reagent (Mirus) in 250 μL Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293 cells were transfected using TransIT LT1 DNA transfection reagent (Mirus, Madison, WI) as described previously (Ladefoged ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug plasmid DNA and 3 uL TransIT-LT1 transfection reagent (Mirus, Cat#2300) were diluted in 100 uL Opti-MEM Reduced-Serum Medium (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... A ratio of 1ug DNA to 3ul TransIT LTI (Mirus Bio, Madison, WI, USA) was used to transfect cells with 20.8ng VP35 ...
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bioRxiv - Immunology 2021Quote: Human Alveolus Chips were transfected with plasmid DNA using the TransIT-X2 reagent (Mirus Bio). 3 ug of plasmid DNA and 6 ul TransIT-X2 reagent were constituted in 300 ul OPT-MEM before added to 3 ml chip flow medium ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: Transient DNA transfections were carried out using a TransIT-HeLaMONSTER® kit (Mirus Bio LLC), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... to a final concentration of 10 μg DNA/well using Mirus TransIT-lnsect (Mirus Bio). 48 h post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila constructs were transfected with plasmid DNA using TransIT®-Insect transfection reagent (Mirus Bio).