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Citations for Mirus Bio :
51 - 93 of 93 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... All transfections used 2.49 µg plasmid DNA with 6.25 µL TransIT LT1 transfection reagent (Mirus, Madison, WI) in 250 µL Opti-MEM (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells containing RNP and donor DNA were electroporated at 150V using the Ingenio EZporator Electroporation System (Mirus). Cells were incubated at ambient temperature for 5 min and transferred to a six-well plate containing 2 mL growth medium with or without combinations of 1 μM AZD7648 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5 million MEFs and 5µg total DNA were diluted in 100µL of Ingenio Solution in a 0.2cm cuvette (Mirus). MEFs were nucleofected using AMAXA program A-023 (MEF) ...
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bioRxiv - Immunology 2023Quote: ... These DNA mixes were incubated with 18 mL OptiMEM and 1 mL of TransIT-LT1 reagent (Mirus) for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... MmAtxn10::EGFP-N1 plasmids were transfected into cells using TransIT®-2020 DNA per manufacturer guidelines (Mirus, MIR5404).
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bioRxiv - Microbiology 2020Quote: ... Cells were transiently transfected with 500ng of total DNA and 1.5μl of Transit X2 (Mirus Bio, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 10g of plasmid DNA was transfected into 293T lentiviral packaging cells using LT1 transfection reagent (Mirus Bio LLC), and viral supernatants were collected ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 2 μg of DNA 24 - 48 hours before measurement using TransIT transfection reagents (Mirus). DRG neurons from adult (postnatal weeks 8–12 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 10 µg of DNA at 70-80% cell density using TransIT-LT1 (Mirus, 2300) for 293T cells and at 50-60% cell density with Lipofectamine 3000 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: Viral particles were produced by transfection of HEK293FT cells in 6-well plates with 3µg DNA and 8µl TransIT-293 (Mirus Bio) per well ...
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bioRxiv - Immunology 2022Quote: Viral particles were produced by transfection of HEK293FT cells in 6-well plates with 3μg DNA and 8μL TransIT-293 (Mirus Bio) per well ...
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bioRxiv - Cell Biology 2020Quote: Transient DNA transfections of wild-type AP4B1 were carried out using a TransIT-HeLaMONSTER® kit (Mirus Bio LLC), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and transfected with 1.5 µg of the indicated plasmid DNA and 0.5 µg of the PiggyBac transposase construct using TransIT-X2 (MIR6004, Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... the cells were plated in a 12.5 cm2 dish and were transfected at 60% confluency with 0.8 µg DNA using TransIT-293 (Mirus, MIR2700). We used a GFP-tagged and C-terminally truncated human HCN1 construct which was cloned into a pEG plasmid as previously reported by Lou & MacKinnon (2017) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of total plasmid DNA and 1.5 μL of TransIT ®-2020 Transfection Reagent (Mirus Bio, WI, USA) were mixed by gentle pipetting ...
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bioRxiv - Cancer Biology 2023Quote: ... were done by mixing 2.5 µg of plasmid DNA with 7.5 µl of TransIT-X2 reagent (Mirus, Madison, WI). The mixture was added to 105 CAF or PDAC cells cultured in serum-free and antibiotic-free media overnight.
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bioRxiv - Biochemistry 2024Quote: ... and TRUPATH plasmids at 1:1:1 DNA ratio (Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at ∼60% confluency with 2.5 µg of the appropriate DNA construct using the TransIT-LT1 Transfection Reagent (Mirus; MIR2300) and cultured for an additional 2 days ...
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bioRxiv - Microbiology 2019Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
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bioRxiv - Microbiology 2019Quote: ... MHV68 was produced by first making p0 virus by transfecting NIH 3T3 cells in 6-well plates with 2.5 μg BAC DNA using TransIT-X2 (Mirus Bio) for 24h ...
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bioRxiv - Neuroscience 2021Quote: TDP-43 inducible knockdown SH-SY5Y cells were electroporated with 2 μg of DNA with the Ingenio electroporation kit (Mirus) using the A-023 setting on an Amaxa II nucleofector (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were transiently transfected with 500 ng of total DNA and 1.5 µl of Transit X2 (Mirus Bio, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: H1 control AC16 cardiomyocytes and cGAS KO AC16s were seeded in 12-well plates and transfected with 5 mg of CT-DNA using Transit X2 (Mirus) at a 2:1 ratio for 8 h prior to harvest ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 250 ng of DNA (split evenly by mass between the plasmids indicated) using TransIT-LT1 (Mirus). Cells were either incubated for 24 h (untreated) ...
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bioRxiv - Microbiology 2022Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Retrovirus was generated by transfecting the Plat-E packaging cell line with 2.5 μg DNA and 7.5 μl TransIT-X2 Reagent (Mirus, MIR 600). B cells were isolated and cultured in complate 1640 RPMI medium before transduction ...
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bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Microbiology 2023Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg of total DNA and 8 mL TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Immunology 2022Quote: Monolayers of BSC-40 cells were infected with parental IHD-J at an MOI of 0.01 PFU/cell and transfected 1 hour later with 10 μg of DNA of plasmid pCyA-ISG15GG (pCyA was kindly provided by Mariano Esteban) using LT-1 (Mirus Bio) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg DNA and 8 μl TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Synthetic Biology 2022Quote: ... and transfected with a total of 2.5 μg of SSG-encoding DNA/well using TransIT-2020 Transfection Reagent (Mirus Bio, #MIR5400) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... 80% confluent monolayers of Vero E6 cells in 12-well plates were transfected with 1.0 μg per well of infectious SARS-CoV-2 BAC DNA or its mutated derivatives using TransIT®-LT1 transfection reagent according to manufacturer’s instructions (Mirus Bio). At 48 h post transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were passaged to 12-well tissue culture plates at a density of 1.5×105 cells/well (3.9×104 cells/cm2) and transfected with 500 ng plasmid DNA using 2 uL of TransIT-X2 transfection reagent (Mirus Bio, #MIR6005) and 100 uL Opti-MEM I reduced serum medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: HIV particles were produced by transfection of HEK293T cells with viral plasmid DNA using Mirus LT1 reagent (Mirus Bio LLC, Madison WI). At 24h ...
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bioRxiv - Cell Biology 2021Quote: ... Sleeping Beauty system was applied as described in the plasmid DNA transfection protocol from TransIT-X2® Dynamic Delivery System (Mirus Biotech®) with modifications ...
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bioRxiv - Microbiology 2021Quote: ... cells were transiently transfected with 19 μg pNL4-3 or the respective proviral DNA using TransIT®-LT1 transfection reagent (Mirus Bio LLC) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1 µg pSINrep21/YFV NS1 plasmid DNA was transfected into BHK-21 clone 15 cells by using 8 µl TransIT LT1 reagent (Mirus Bio, Wisconsin, MD) and low-serum OptiMem (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... were grown in 6-well plates and transfected the next day with 2.5 μg DNA with TransIT-LT1 (Mirus Bio catalog number MIR-2304) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were seeded into 14.5 cm dishes and transfected with 40 µg pTK-SM-N100-GFP-V5 expression vector using TransIT-2020 (Mirus Bio; 1 µg DNA: 2 µL reagent), delivered in Opti-MEM ...