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Citations for Mirus Bio :
201 - 250 of 294 citations for Cortisone Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Following 1 week of incubation with a selection media containing G418 (Mirus Bio LLC) at 1mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... AMO1 shIRE1 cl.1 cells were transfected using TransIT-X2 Dynamic Delivery system (Mirus), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug plasmid DNA and 3 uL TransIT-LT1 transfection reagent (Mirus, Cat#2300) were diluted in 100 uL Opti-MEM Reduced-Serum Medium (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... primary cortical neurons were transfected by nucleofection using Nucleofector™ II (Amaxa) and the Ingenio® Electroporation kit (Mirus Bio LLC). For one well of a 6-well plate ...
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bioRxiv - Biochemistry 2022Quote: ... in 12-well plates and were transfected with 3 μg capped in vitro transcribed DENV replicon RNA using the TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... scDNAs were Cy5-labeled using the Label IT® Nucleic Acid Labeling Kit (Mirus Bio LLC, Madison, WI, USA; WI 53719), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed with 2 × SSC three times and then the nucleic acids in the samples were amine-modified using a Label IT Amine modifying kit (Mirus Bio) for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cy5-labeled probes were generated from RP11-440N18 BAC DNA sonicated to 150 bp and labeled using a DNA labeling kit (Mirus Bio). Slides were denatured with 0.5 M NaOH for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transfection of cells seeded in a T25 flask at a confluency of ≥80% was performed using TransIT®-CHO Transfection Kit (MIR 2174, Mirus Bio), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the packaging cell line HEK293-FT was transfected with pTCP-MPsfGFP together with pSAX2 (12260 Adgene) and pCMV-VSV-G (8454 Adgene) plasmids using the kit TransIT®-293 Transfection Reagent (Mirus Bio) and following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... culture medium was replaced with 345 µL fresh DMEM per well and mammalian expression plasmids were transfected into cells using the TransIT-HeLaMONSTER Transfection Kit (Mirus Bio #MIR2904) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... or non-Targeting shRNA vectors pLKO.1 using Mirus Trans-IT TKO Transfection reagent (Mirus). 621-101 cells were transduced with lentiviruses for 48 hours and then selected against puromycin ...
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bioRxiv - Molecular Biology 2020Quote: ... Transient transfection with synthetic mRNA of Cas9 and sgRNAs was performed in either MEF or 293K cells using the TransIT®-mRNA Transfection Kit (Mirus Bio LLC) in OptiMEM medium (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... or control XRNA (RNA segment derived from the hepatitis C virus genome adjacent to and equivalent in length to polyU/UC that cannot induce RIG-I-dependent innate immune signaling8) using the TransIT®-mRNA Transfection kit (Mirus Bio LLC). Cells were collected in RLT buffer (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 μg of PiggyBac transposase expression vector (total = 3 μg) using TransIT-LT1 (Mirus Bio) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and a Venus-tagged N-terminal β-arrestin2 using 3:1 ratio of TransiT-2020 (Mirus). 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs) ...
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bioRxiv - Immunology 2022Quote: ... plasmid was fluorescently labeled with the Cy5 fluorophores using the Mirus Label IT® tracker intracellular nucleic acid localization kit (MIR 7021, Mirus Bio, Madison, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... The ctDNA was fluorescently labeled with either Cx-rhodamine or CY5 using the Label IT® Nucleic Acid Labeling kit (Mirus Bio LLC, WI).
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bioRxiv - Synthetic Biology 2023Quote: ... 2.5x105 wildtype K562 cells and 2.5x105 reporter K562 cells were mixed for a total of 5.0x105 cells per well and transfected with various amounts of the purified mRNA using the TransIT®-mRNA Transfection Kit (Mirus Bio, catalog# MIR2250). To induce the reporter cell lines ...
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bioRxiv - Molecular Biology 2023Quote: The Cas9-sgRNA and Cas12a-crRNA were labeled with CX-rhodamine (Label IT® Nucleic Acid Labeling Reagents Rhodamine Kit, Mirus Bio, Madison, WI) (75 ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3:1 volume:mass ratio of Mirus TransIT®-LT1 transfection reagent (Mirus Bio LLC, Madison WI) to DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... and VSV-G vectors at a 4:2:1 ratio with Mirus TransIT LT1 (Mirus Bio, LLC). Virus-containing supernatant was collected 48 and 72h after transfection and filtered through 0.45mm filters before concentration by ultracentrifugation (25,000 RPM for 2 hours with low decel) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were co-transfected with a lentiviral sgRNA expression plasmid and the packaging vectors pCMVΔ8.91 and pMD VSV-G (1:0.7:0.3 mix) using TransIT-293 (Mirus Bio). HEK293T media was collected 48 h post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... The CPER products were then directly transfected into HEK293-C34 cells using Trans IT LT-1 (Mirus). At 6 hours post-transfection ...
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bioRxiv - Cell Biology 2019Quote: ... 8 µg pCMV-dR8.91 and 1 µg pMD2.G packaging plasmids using 48 µl TransIT-LT1 (Mirus). Plasmid DNA was mixed with transfection reagent in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected with the plasmids by using Trans IT LT-1 transfection reagent (Mirus, Madison, WI) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... or 2:2:1 (33) with TransIT®-LT1 transfection reagent (Mirus Bio LLC, Madison, WI, USA) and the plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... seeded on day 0 in a T75 culture flasks and transfected on day 1 with TransIT (Mirus) using 3rd generation packing plasmids (pHDMG·G VSV ENV ...
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bioRxiv - Immunology 2023Quote: ... These DNA mixes were incubated with 18 mL OptiMEM and 1 mL of TransIT-LT1 reagent (Mirus) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... Using either the DharmaFECT 1 Transfection Reagent (T-2001-02, Dharmacon) or the Mirus TransIT-X2® (Mirus) system as per the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 μg mL−1 Streptomycin were passed to 10 cm dishes and co-transfected using TransIT (Mirus Bio) in an approximate 1:2.5 ratio with DRD1 containing C-terminal Renilla luciferase (RLuc ...
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bioRxiv - Genomics 2019Quote: ... 4 to 8-week-old mice were injected intravenously with 1 μg of mRNA formulated with TransIT transfection reagent following the manufacturer’s suggestions (Mirus). The procedure for one mouse was as follows ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 μg of PiggyBac transposase expression vector (total = 3 μg) using TransIT-LT1 (Mirus Bio, Madison, WI, USA) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: A549 ZAP−/−/− and DF-1 cells were cultured in 10 cm dish and transfected using LT1 reagent (Mirus lab) with 5 μg of huZAP-GFP DNA for 24 h ...
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bioRxiv - Neuroscience 2020Quote: ... and mRFP + Nuak 1 and 2 kinases was performed via chemical transfection using TransIT-X2 (Mirus Bio, Madison, WI) 48 hours following plating ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 Mϕ were transfected with a transfection complex (50 μl) of 0.3% TransIT™-LT1 (Mirus Bio™) transfection reagent (vehicle ...
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bioRxiv - Cell Biology 2022Quote: ... Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus), and Opti-MEM Reduced Serum Medium (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... cultured in Schneider’s Drosophila Medium containing 10% heat-inactivated FBS and 1X penicillin-streptomycin with the TransIT-LT 1 reagent (Mirus). Flag-CG30403 only (no bait ...
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bioRxiv - Cell Biology 2020Quote: ... adherent Sf21 cells were transfected with plasmids expressing ARIF-1 using TransIT-Insect transfection reagent (Mirus Bio, Madison, WI). At 3 d post-transfection ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep) were transfected with 25 μg of plasmid encoding for the corresponding S glycoprotein using TransIT-Lenti (Mirus) as transfectant reagent ...
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bioRxiv - Biophysics 2022Quote: ... and 100 ug ml-1 Streptomycin were passed to 10 cm dishes and co-transfected using TransIT (Mirus Bio) in an approximate 1:2.5 ratio with NTSR1 containing C-terminal Renilla luciferase (RLuc ...
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bioRxiv - Cancer Biology 2023Quote: ... Control and BCAT1-KO U251 cells were reverse-transfected with 1µg/ml of TC3-R9P-3NLS pDNA using 1:2 of Trans-iT (Mirus). 48h after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... pTRIPz or pLKO.1 plasmid was co-transfected with lentiviral Packaging vectors (pMD2.G and psPAX2) into HEK293T using TransIT-LT1 (Mirus) transfection reagent following the manufacturer instruction ...
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bioRxiv - Genomics 2021Quote: ... and pMD2.G at a ratio of 9:8:1 by mass using TransIT-LT1 transfection reagent (Mirus MIR 2306) at a ratio of 3 μL transfection reagent per 1 μg plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... lentiviral particles were generated by transient transfection of the lentiviral packaging Lenti-X 293T cells (Takahara) with the TCR encoding plasmids and packaging plasmids (VSVg and PSPAX2) 25 using Transit LT-1 (Mirus). Lentiviral supernatant was harvested 2 days later ...
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bioRxiv - Biophysics 2020Quote: Plasmids for expression of WT or mutant rat KIF1A(339)-LZ motors tagged with monomeric NeonGreen and an FRB domain were cotransfected into COS-7 cells with a plasmid for expression of PEX3-mRFP-FKBP at a ratio of 6:1 with TransITLT1 transfection reagent (Mirus). Eight hours after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral particles were produced by co-transfection of shRNA plasmids with psPAX and pMDG.2 in 293T cells using the Trans-IT LT-1 transfection reagent (Mirus). DM cells were infected by shRNA lentivirus twice via spinoculation in the presence of 5 μg/mL polybrene (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... 1 x 106 Jurkat cells expressing Strep-tagged ADAP1 or GFP were resuspended in 0.1 mL Mirusbio ingenio (Mirus, MIR50115) solution containing 2 μg of TF-responsive reporters driving Firefly luciferase (pGL3-3xAP1-luciferase (Addgene ...