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Citations for Mirus Bio :
101 - 150 of 195 citations for Corticosterone ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Monoclonal and polyclonal hIPSC-dCas9 lines were transfected with pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep or pCS2-iREP-GFP-PGK-BFP-U6-gRNA-mock vectors as replicates in 12 well plates using TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR2300) according to manufacturer’s instructions and as detailed above ...
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bioRxiv - Microbiology 2022Quote: 3×105 cells were seeded in 6-well plates and transfected with 3CLpro plasmids via TransIT-PRO (Mirus Bio LLC, Madison, WI, USA) and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded in 10-cm dishes or 6-well plates and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... were transfected with 2·5 µg serine protease expression plasmid or empty vector using TransIT-X2 Dynamic Delivery System (Mirus). One day later ...
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bioRxiv - Cell Biology 2022Quote: Plasmids for expression of kinesin-1 or any of the kinesin-6 family motors tagged with monomeric NeonGreen and an FRB domain were cotransfected into COS-7 cells with a plasmid for expression of PEX3-mRFP-FKBP or GMAP210p-mRFP-2xFKBP at a ratio of 5:1 with TransIT-LT1 transfection reagent (Mirus). Eighteen hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were transfected with 10 ug of the TRE library ± 5 ug of pcDNA3.1 containing a GPCR expression cassette using TransIT-2020 (Mirus Bio) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Medium was changed 24 h later to 10 ml of 5 % FBS/DMEM and changed again 48 h later to 10 ml of 5 % FBS/DMEM containing 1 µg/ml of puromycin (Mirus). Cells were incubated in selection medium for 72 h ...
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bioRxiv - Pathology 2021Quote: ... Ad293 cells were cultured in 96 well plates with DMEM containing 10% FB Essence (Avantor Seradigm, USA) and transfected using Transit-LT1 (Mirus Bio, Madison, WI, USA) with plasmid encoding 4 LXR response elements fused with luciferase ...
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bioRxiv - Cell Biology 2023Quote: ... was then transfected into SH-SY5Y cells (50-60% confluent) in a 6-well plate using TransIT LT-1 reagent (Mirus Bio, Cat# MIR-2300). After 48 hr ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach 50%-70% confluency before transient transfection using TransIT-2020 (Mirus Bio, catalog #: MIR 5400) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2023Quote: ... were grown in 6-well plates and transfected the next day with 2.5 μg DNA with TransIT-LT1 (Mirus Bio catalog number MIR-2304) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Using the TransIT-mRNA Transfection Kit (Mirus, Madison, WI), 1 × 107 293T cells were transiently transfected with mRNA (20 μg) ...
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bioRxiv - Cell Biology 2021Quote: Biotin was covalently coupled to pDNA (pGL3 plasmid) by using Nucleic Acid Labeling Kit according to manufacturers’ protocol (Label IT® Nucleic Acid Labeling Kit, Biotin, Mirus Bio, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 293T cells were seeded in 24 well plates at 2×104 cells per plate and then transfected the next day with pYX014 or pYX017 (100 ng) using TransIT-LT1 transfection reagent (Mirus Bio LLC, Madison, WI53711, USA). All transfections were performed using equal number of tested plasmid DNA molecules ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... The VSV-G pseudotyped lentivirus was packaged by 5 plasmid co-transfection of HEK293T cells using TansIT Transfection Reagent (Mirus Bio) in 15 cm culture dishes (The protocol describing production of lentiviral particles can be found at http://www.www.bu.edu/dbin/stemcells/protocols) ...
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bioRxiv - Microbiology 2020Quote: ... were seeded in 10-cm dishes at a density of 1e5 cells/cm2 and the following day transfected with 5 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were seeded in 10-cm dishes at a density of 1e5 cells/cm2 and the following day transfected with 5 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... were labeled with Cy3 using a Label IT kit (Mirus). B16F10 cells were transfected with labeled sgRNAs and Cas9 protein (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10 cm dishes and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus Bio, Madison, WI, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected with 25 nM scramble or PLIN 5 siRNA (Dharmacon, Lafayette, CO, USA) using TransIT-TKO (Mirus Bio LLC) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... RNA transfections were performed with TransIT-mRNA Transfection Kit from Mirus Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... using TransIT-HeLaMONSTER transfection kit according to the manufacturer’s protocol (Mirus). Cells were subsequently incubated in the presence of 50 ng/ml tetracycline to inhibit expression of the target mRNA ...
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bioRxiv - Immunology 2020Quote: ... and then co-transfected using TransIT-mRNA Transfection Kit (Mirus Bio) with a GFP-expressing mRNA plasmid (Miltenyi Biotec ...
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bioRxiv - Biophysics 2021Quote: ... RNA was Cy3 labeled using a Label IT kit (Mirus Bio), and RNA purity was verified using a 0.8% agarose gel ...
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bioRxiv - Immunology 2023Quote: ... mtDNA was biotinylated with Label IT Nucleic Acid Labeling Kits (Mirus) and fragmented with HaeIII (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... siRNA transfections were performed with TransIT-X2 Transfection Kit (Mirus Bio) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... In vitro transcribed RNAs were transfected using TransIT-mRNA transfection kit (Mirus). siRNAs directed against CMTR1 (Dharmacon ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected using the TransIT-CHO Transfection Kit (Mirus Bio LLC) (CHO-K1 ...
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bioRxiv - Neuroscience 2023Quote: ... H1 plasmid library with the top 5 sgRNA per gene21 were transfected into HEK293s using the TransIT®-Lenti Transfection Reagent (Mirus, Cat. No. MIR 6606) along with third generation lentiviral packaging mix ...
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bioRxiv - Immunology 2022Quote: ... RNA PAMP transfections were done using the TransIT-mRNA Transfection kit (Mirus Bio). The siRNA transfections were done using Lipofectamine RNAiMax (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... PMA-differentiated U937 cells were then transfected with TransIT mRNA transfection kits (Mirus) according to the manufacturer protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Transfection was performed using the TransIT-PRO® Transfection Reagent & Kit (Mirus Bio). Cells were incubated at 28°C following transfection for 48h ...
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bioRxiv - Neuroscience 2023Quote: ... XGal staining was performed using the beta-galactosidase staining kit (Mirus #MIR 2600), following the provided protocol ...
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bioRxiv - Genomics 2019Quote: ... The plasmids were electroporated into K562 cells with Ingenio Electroporation Kit (Mirus, MIR 50115). For each electroporation ...
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bioRxiv - Immunology 2019Quote: ... RIG-I agonist transfections were done using the TransIT-mRNA Transfection kit (Mirus Bio). The siRNA transfections were done using Lipofectamine RNAiMax (Invitrogen) ...
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bioRxiv - Plant Biology 2022Quote: ... the purified miRNAs were labeled with Cy5 fluorophore (Cy5 Labeling Kit; Mirus Bio LLC) and then subjected to hybridization with the customized microarray for 4 hours in a 42°C oven ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with Tat mRNA using the Mirus TransIT-mRNA transfection kit (Mirus). Cells that produced mCherry signal after transfection were isolated from the array and isolated as monoclonal populations into 96-well plates for expansion.
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bioRxiv - Immunology 2021Quote: ... Baculovirus was generated using the flashBac™ kit according to the manufacturer’s instructions (Mirus Bio). The protein was expressed in 2 L of Sf9 cells at 2×106 cells/mL maintained in ESF921 media (Expression Systems ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: Transient DNA transfections were carried out using a TransIT-HeLaMONSTER® kit (Mirus Bio LLC), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of cells with mRNAs was achieved using a TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase reporter mRNAs were transfected into these cells using the TransIT-mRNA Transfection Kit (Mirus), with the following protocol modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA-RNA was covalently labeled with Cy3 using a LabelT-Cy3 kit (Mirus Bio MIR 3625) following the manufacturer instructions.
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bioRxiv - Neuroscience 2022Quote: ... was transfected into BHK cells with TransIT-mRNA Transfection Kit (Mirus Bio, Cat. No. MIR 2225). Virus containing supernatant was concentrated ...
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bioRxiv - Immunology 2020Quote: ... self-RNA was labeled using a Label IT® Nucleic Acid Labeling Kit (Mirus cat. MIR7125), and Spermidine was labeled with 2-(methylamino ...
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bioRxiv - Microbiology 2021Quote: ... was labelled with Cy5 by following manufacture’s protocol (Mirus Label IT Nucleic Acid Labeling Kit Cy5), diluted to 80 μg mL-1 in TE buffer ...
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bioRxiv - Immunology 2020Quote: ... NK-92 cells were transfected with mRNA via TransIT®-mRNA Transfection Kit (Mirus Bio LLC) according to the manufacturer’s recommended protocols ...
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bioRxiv - Immunology 2022Quote: ... and mRNA-1285 at various concentrations using a TransIT mRNA transfection kit (Mirus Bio, Madison, WI). At 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... or BVDV IRES-driven Fluc mRNA reporters using TransIT®-mRNA Transfection Kit (Mirus Bio MIR 2250) at a 1:2 (µg to µl ...