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Citations for Mirus Bio :
101 - 150 of 180 citations for CD86 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 0.08 million cells/well were seeded in a 24 well plate and transfected with TransIT-293 (Mirus) with 0.5µg/well of either pTRIP-SFFV-Hygro-2A-mScarlet ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cell line was used for retroviral and lentiviral system amplification with TransIT-293 Transfection Reagent (Mirus).
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bioRxiv - Microbiology 2023Quote: HEK293 were transfected with pMTBS and pCS2-ST at ~80% confluence using TransIT-293 transfection reagent (Mirus). Cells were harvested for downstream applications 48 hours post-transfection.
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral supernatants were generated by co-transfection of HEK-293T cells using Transit-X2 (Mirus; #MIR 6004) with a 3-vector lentiviral system ...
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bioRxiv - Cell Biology 2019Quote: ... 293T cells were co-transfected in a 1:1 ratio with a lentiviral expression vector (pHRSIN/pHRSiren/pKLV) and the packaging vectors pMD.G and pCMVR8.91 using TransIT-293 (Mirus). Supernatant was harvested at 48h post-transfection and transferred onto target cells ...
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bioRxiv - Microbiology 2020Quote: ... 13 μg psPAX2 and 20 μg pTRIP-SFFV-eGFP-NLS and 114 ul of TransIT-293 (Mirus Bio). One day after transfection ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated at ~20-50% confluence and transfected the following day using TransIT-293 transfection reagent (Mirus) and OPTI-MEM (1105802 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 1.5 µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167 µg of pMD2.G and 1.3 µg of p8.91) with TransIT-293 (Mirus Bio). After 2–3 d of transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Phoenix Ecotropic (pECO) cells (ATCC CRL-3214) were transfected with plasmids using TransIT-293 transfection reagent (MIR2705, Mirus). Briefly ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells seeded in a 10 cm dish were transfected using TransIT-293 (Mirus Bio, Madison, WI, USA) with 670 ng pCAGGS-GP ...
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bioRxiv - Cancer Biology 2021Quote: ... and pMIG-luciferase-IRES-GFP was produced by transfection of 293T cells with TransIT-293 (Mirus Bio, Madison, WI), lentiviral backbone as well as packaging vectors Δ8.9 (gag/pol ...
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bioRxiv - Cell Biology 2020Quote: ... together with the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at a 10:7.5:5 ratio using TransIT-293 reagent (Mirus). The supernatant containing the viral particles was collected 48 hrs after transfection ...
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bioRxiv - Genomics 2019Quote: ... HEK293 cells were transfected with either 2.5 or 8 ug of the pCMV-Aire-Flag plasmid (or control plasmid) using 7.5 or 32 ul of the TransIT-293 transfection reagent (Mirus). After 48h ...
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bioRxiv - Cell Biology 2019Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng of pcDNA3-PLPro-flipGFP-T2A-mCherry plasmid and 50 ng of protease expression plasmid pcDNA3.1-PLpro were added to each well in the presence of transfection reagent TransIT-293 (Mirus) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were transfected with 5 μg of the indicated FLAG-tagged TTP wild-type or mutant expression plasmids using TransIT 293 reagent according to the manufacturer’s protocol (Mirus). 48 hours after transfection ...
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bioRxiv - Immunology 2019Quote: Viral particles were produced by transfection of HEK293FT cells in 6-well plates with 3µg DNA and 8µl TransIT-293 (Mirus Bio) per well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng pcDNA3-flipGFP-T2A-mCherry plasmid and 50 ng protease expression plasmid pcDNA3.1 were used each well in the presence of transfection reagent TransIT-293 (Mirus). 3 hrs after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... plus pMK1221-control or −GR shRNA (2.88 ug shCtrl or shGR, respectively) using TransIT-293 Transfection Reagent (Mirus Bio) as instructed by the manufacturer ...
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bioRxiv - Immunology 2022Quote: Viral particles were produced by transfection of HEK293FT cells in 6-well plates with 3μg DNA and 8μL TransIT-293 (Mirus Bio) per well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng of pcDNA3-flipGFP-PLpro-T2A-mCherry plasmid and 50 ng of pcDNA3.1-SARS2-PLpro were used each well in the presence of transfection reagent TransIT-293 (Mirus) per manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with the N-terminal FLAG-tagged CDC42 expression vector using TransIT 293 (Mirus Bio LLC) in 6-well culture plates ...
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bioRxiv - Cell Biology 2022Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio; Madison, WI). Three days post transduction virus-containing supernatants were harvested and concentrated approximately 40-fold using Lenti-X Concentrator (Clontech ...
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bioRxiv - Biophysics 2023Quote: ... the cells were plated in a 12.5 cm2 dish and were transfected at 60% confluency with 0.8 µg DNA using TransIT-293 (Mirus, MIR2700). We used a GFP-tagged and C-terminally truncated human HCN1 construct which was cloned into a pEG plasmid as previously reported by Lou & MacKinnon (2017) ...
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bioRxiv - Cell Biology 2022Quote: HEK293T cells were transfected with lentiviral vectors (pLVX / pLVX-TetOne) plus packaging plasmids pCMVR8.91 and pMD.VSVG using TransIT-293 (Mirus Bio). Viral supernatants were collected 48 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.2 µg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 transfection reagent (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at 10:7.5:5 ratio using TransIT-293 Transfection Reagent (MIR2704, Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: HEK 293T cells (106) were transfected in 6-well plates with corresponding plasmids with Transit LT1 transfection reagent (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with TCR expression vector and lentiviral transfer plasmids using TransIT-VirusGen Transfection Reagent (Mirus #MIR6700) following the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... were packaged in HEK 293T cells as previously described [15] using Turbofect (Fisher, UK) or trans-IT (Mirus. USA) as lipofection reagents ...
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bioRxiv - Genetics 2021Quote: ... Transfection of 6xMyc-tagged RBPJ constructs (pCS2-RBPJ-6xMyc, WT and AOS variants) was carried out with TransIT-293 transfection reagent (Mirus) following the manufacture’s instruction ...
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bioRxiv - Biophysics 2021Quote: ... δ and ε subunits in the ratio 2:1:1:1 (TransIT® 293 transfection reagent; Mirus Bio, Madison, WI). Electrophysiological experiments started ~48 hours post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... The following day they were transfected with 6 μg Gag plasmid + 6 μg vector plasmid + 3 μg pCMV-Rev (to support nuclear export of vector RNA) using Transit-293 (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Retrovirus was produced by transfection of Lenti-X 293T cells with packaging constructs and viral expression vector using TransIT-293 (Mirus). Viral supernatant was harvested 48hr later transfection and filtered through a 0.45 μM filter ...
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bioRxiv - Immunology 2020Quote: ... pMigR1-IRES-Thy1.1 or pMigR1-Cre-IRES-Thy1.1 plasmid (Feng et al., 2015) and pCl-Eco were cotransfected into these cells with TransIT-293 Transfection Reagent (Mirus); 2-3 days after transfection ...
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bioRxiv - Immunology 2022Quote: ... Cells were then plated at 0.8 million cells/well in a 6 well plate and transfected with either pTRIP-SFFV-Hygro-2A-mScarlet-HGS or pTRIP-SFFV-Hygro-2A-mScarlet-VPS37A with TransIT-293 (Mirus) (3µg DNA/well) ...
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bioRxiv - Immunology 2022Quote: ... and pCMV-RD8.9 (a kind gift from Donald B. Kohn) were transfected into HEK293T cells using TransIT-293 (Mirus Bio) and OPTI-MEM (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK T-REx wild type or SCAPER KO cells were seeded in 12-well plates and transfected the next day with pcDNA5/FRT/TO rescue plasmids and a puromycin-resistance conferring plasmid (MXS-CMV-PuroR) using TransIT-293 (Mirus). 24 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: Lentivirus was generated by co-transfection of HEK293T cells with a desired transfer plasmid and two packaging plasmids (psPAX2 and Δ8.9) using the TransIT-293 transfection reagent (Mirus, USA). 48 h post transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... and the gRNA-expressing plasmid (pC016-gPsp-Control or pC016-gPsp-EGFP start codon, 12 μg) using TransIT-293 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the above-described PiggyBac-derived plasmid was transfected into KO Fhl1 HEK 293T cell line using TransIT-X2 reagent (Mirus). Next day ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 ΔENV pseudotyped with VSV-G were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). For HIV-1 ΔENV ΔVpr with Vpr WT packaged in trans (HIV-1ΔVpr +Vpr WT) ...
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bioRxiv - Microbiology 2024Quote: ... Virus-like particles (VLPs) packaging Vpr WT or mutant proteins were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). VLPs were harvested 48 hrs post transfection ...
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bioRxiv - Cancer Biology 2021Quote: Retrovirus MSCV and MSCV-NKX2-1 and were produced by transfection of HEK293T cells with TransIT-293 (Mirus Bio, Madison, WI) with the retrovirus backbone as well as packaging vectors gag/pol and CMV-VSV-G ...
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bioRxiv - Cell Biology 2022Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg of total DNA and 8 mL TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Molecular Biology 2020Quote: ... to be integrated into the genomic FRT site was cloned into the pcDNA5/FRT/TO vector backbone and transfected together with pOG44 Flp-In recombinase in a 9:1 ratio using either Trans-IT 293 transfection reagent (Mirus, USA) or Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... The cells were transfected with the sgRNA transfer plasmids (MSCV-U6-sgRNA-IRES-Thy1.1) using TransIT-293 transfection reagent per the manufacturer’s protocol (Mirus, Cat# MIR 2700). The following morning ...
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bioRxiv - Molecular Biology 2019Quote: ... a 10 cm dish of cells was transfected with 9 µg of Flp-Recombinase (plasmid pOG44) and 1 µg of a specific pcDNA5/FRT plasmid using TransIT-293 transfection reagent (Mirus, MIR2705). 48 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL and psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL plasmids were transfected into cells with TransIT-293 Reagent (Mirus, MIR2704). After 24 h of incubation ...
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bioRxiv - Cell Biology 2021Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio, MIR 2705). Following transfection ...