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Citations for Mirus Bio :
101 - 150 of 163 citations for 6 methyl 3 oxo 2 3 dihydropyridazine 4 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: HeLa cells inducibly expressing GFP and Flag-HA-tagged UNKWT were seeded in 6-well dishes and transfected 24 hours later at about 40% confluence using the TransIT-X2 Dynamic Delivery System (Mirus, MIR6003) with a pool of siRNAs targeting CNOT7 (Horizon ...
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bioRxiv - Systems Biology 2020Quote: ... followed by incubation with 10fold diluted Label IT Amine Modifying Reagent in 1× Labeling Buffer A from Label IT Nucleic Acid Modifying Reagent (Mirus Bio MIR 3900) at room temperature for 45 minutes ...
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bioRxiv - Systems Biology 2021Quote: ... followed by incubation with tenfold diluted Label IT amine modifying reagent in 1× labelling buffer A from Label IT nucleic acid modifying reagent (Mirus Bio MIR 3900) at room temperature for 45 minutes ...
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bioRxiv - Cell Biology 2022Quote: siRNA was delivered to differentiated adipocytes (6-7 days post-differentiation) using the TransIT-X2® dynamic delivery system (MIR 6004; Mirus Bio). Opti-MEM I (31985062 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach ~70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: 5400), or siRNA treatment (50 nM ...
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bioRxiv - Molecular Biology 2020Quote: ... MEF cells were grown in 6-well plates to 80% confluency before transfection with RP-sgRNA11204G (1.5ug/well) using TransIT®-mRNA Transfection Kit (Mirus Bio LLC). After 24h post transfection ...
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bioRxiv - Microbiology 2020Quote: ... Monolayers of BHK-T7 cells at ~50% confluency in 6-well plates were co-transfected using TransIT-LT1 transfection reagent (Mirus Bio LLC) with 0.8 μg of each of ten plasmid constructs representing the T1L or T3DI reovirus genome ...
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bioRxiv - Microbiology 2023Quote: 3 x 105 cells were seeded per well in 6-well plates and transfected one day after seeding with 3CLpro plasmids using TransIT-PRO (Mirus Bio LLC) and incubated 8-9 hours for Mpro-On and 10 hours for Mpro-Off assays ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 x 105 293T cells per well were seeded in 6-well plates and transfected one day later with GFP-Mpro-L plasmids using TransIT-pro (Mirus Bio LLC). After 10 hours ...
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bioRxiv - Biophysics 2019Quote: ... constructs containing an amber stop codon (TAG) at amino acid position 1353 or 1397 (1353stop or 1397stop) using TransIT transfection reagent (Mirus Bio LLC; Madison, WI) in a ratio of 3 µL of TransIT per µg of total DNA ...
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bioRxiv - Immunology 2022Quote: ... plasmid was fluorescently labeled with the Cy5 fluorophores using the Mirus Label IT® tracker intracellular nucleic acid localization kit (MIR 7021, Mirus Bio, Madison, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... The ctDNA was fluorescently labeled with either Cx-rhodamine or CY5 using the Label IT® Nucleic Acid Labeling kit (Mirus Bio LLC, WI).
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bioRxiv - Molecular Biology 2023Quote: The Cas9-sgRNA and Cas12a-crRNA were labeled with CX-rhodamine (Label IT® Nucleic Acid Labeling Reagents Rhodamine Kit, Mirus Bio, Madison, WI) (75 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded in 10-cm dishes or 6-well plates and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... using 2 μL of TransIT-Insect transfection reagent (Mirus Bio Cat #6104). Medium was replaced with serum-containing medium 5 hours after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... were seeded in 6-well dishes and transfected with siRNA (50 nM) after 24 hours using the TransIT-X2 lipid reagent (Mirus Bio cat. no. MIR6000) or RNAiMAX™ ...
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bioRxiv - Cell Biology 2023Quote: ... was then transfected into SH-SY5Y cells (50-60% confluent) in a 6-well plate using TransIT LT-1 reagent (Mirus Bio, Cat# MIR-2300). After 48 hr ...
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bioRxiv - Microbiology 2023Quote: ... were grown in 6-well plates and transfected the next day with 2.5 μg DNA with TransIT-LT1 (Mirus Bio catalog number MIR-2304) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... with ∼ 600ng DNA and 2 µL TransIT-LT1 transfection reagents (Mirus Bio Corporation) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... 2 µg of plasmid DNA was transfected per dish using Transit 293T (Mirus) following the manufacturer’s instructions and measured at least 48 h later.
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10 cm dishes and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus Bio, Madison, WI, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Biophysics 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Microbiology 2022Quote: ... monolayers of BHK-T7 cells (4×105) cultured in 12-well plates were co-transfected using 2.5 μl of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Microbiology 2019Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Microbiology 2020Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Biophysics 2022Quote: ... with 600 – 1000 ng DNA and 2 μL TransIT-LT1 transfection reagents (Mirus Bio Corporation) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 250 μl of OptiMEM media and supplemented with 2 μl TransIT-2020 Transfection Reagent (Mirus, MIR5400). The transfection mix was incubated 20 min at room temperature and then added to the cell culture for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2 cells were transfected with lentiviral DNA following the Mirus-LT1 (Mirus MIR 2300) transfection protocol ...
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bioRxiv - Microbiology 2019Quote: ... and varying input (50 ng for 5-site screen and 15 ng for 4-site screen) of MxA plasmids were co-transfected into HEK 293T cells using the reagent TransIT-LT1 (Mirus Bio) in a 96-well plate format ...
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bioRxiv - Cell Biology 2022Quote: ... Szczesny) and 0.8 μg EGFP-CHIP plasmid using Mirus reagents: 2 ul TransIT-293 (MIR 2700, Mirus) for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900 ...
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bioRxiv - Genetics 2023Quote: ... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25% ...
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bioRxiv - Genetics 2023Quote: ... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v3 lentiviral sgRNA library at a target infection rate of 10% ...
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bioRxiv - Cell Biology 2020Quote: ... transfected at confluence (Day -2) using Mirus TransIT-X2 transfection reagent (3ul per well; Mirus Bio LLC, US) and 250 ng plasmid DNA (either empty vector or FABP4 sgRNA) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 2 μg of DNA 24 - 48 hours before measurement using TransIT transfection reagents (Mirus). DRG neurons from adult (postnatal weeks 8–12 ...
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bioRxiv - Cell Biology 2022Quote: ... for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900, Mirus) for HeLa Flp-In T-REx cells ...
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bioRxiv - Neuroscience 2020Quote: ... and mRFP + Nuak 1 and 2 kinases was performed via chemical transfection using TransIT-X2 (Mirus Bio, Madison, WI) 48 hours following plating ...
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bioRxiv - Microbiology 2020Quote: BHK-21 cells were seeded in 48-well plates at 5×104/well in DMEM-10% one day prior to transfection with 500ng of different species ACE2-expression constructs or empty vector (pDISPLAY) (Sup.Table.2) in OptiMEM and TransIT-X2 (Mirus) transfection reagent according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid encoding for SARS-CoV-2 S-glycoprotein (YP_009724390.1) harboring a C-terminal 19 aa truncation using TransIT-Lenti (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Caco-2 cells were plated (10·106 cells) in 500μl of the Ingenio electroporation solution (Mirus Vio, Wisconsin, USA). The transfected cells were selected using the antibiotic geneticin/G418 (Gibco-Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Control and BCAT1-KO U251 cells were reverse-transfected with 1µg/ml of TC3-R9P-3NLS pDNA using 1:2 of Trans-iT (Mirus). 48h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... were transfected with 2·5 µg serine protease expression plasmid or empty vector using TransIT-X2 Dynamic Delivery System (Mirus). One day later ...
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bioRxiv - Biophysics 2021Quote: ... δ and ε subunits in the ratio 2:1:1:1 (TransIT® 293 transfection reagent; Mirus Bio, Madison, WI). Electrophysiological experiments started ~48 hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: TDP-43 inducible knockdown SH-SY5Y cells were electroporated with 2 μg of DNA with the Ingenio electroporation kit (Mirus) using the A-023 setting on an Amaxa II nucleofector (Lonza) ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral particles were produced by co-transfection of shRNA plasmids with psPAX and pMDG.2 in 293T cells using the Trans-IT LT-1 transfection reagent (Mirus). DM cells were infected by shRNA lentivirus twice via spinoculation in the presence of 5 μg/mL polybrene (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... and pZIP-mCMV-ZsGreen-shRNA NT Control or mTOR plasmids (2 µg; Transomic) using the TransIT-LT1 transfection reagent (Mirus) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... purified using a Nucleospin kit (Machery-Nagel) and transfected into 2 mL of 293F cells (0.5 mL/mL) using TransIT-Lenti (Mirus bio). The virus was amplified ...
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bioRxiv - Genomics 2023Quote: ... 4ug of packing plasmids psPAX.2 and 2ug of the envelope vector pMD2.G diluted in OptiMEM medium and Trans-Ltl transfection reagent (Mirus). For the generation of gRNA lentivirus ...