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Citations for Mirus Bio :
351 - 400 of 510 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... cells were transfected with 25mM EP300 siRNA or scramble control siRNA via the TransIT-X2 Dynamic Delivery System (Mirus Bio), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells in 6 well plates were transfected with 500 ng/well of each protein-coding plasmid by using TransIT (Mirus). After 48 h ...
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bioRxiv - Microbiology 2019Quote: ... NL4-3 and NL4-3(AD8) HIV viral stocks were produced by transfection of HEK293 cells with the molecular clone plasmids using TransIT-LT1 (Mirus) transfection reagent ...
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bioRxiv - Microbiology 2019Quote: ... MHV68 was produced by first making p0 virus by transfecting NIH 3T3 cells in 6-well plates with 2.5 μg BAC DNA using TransIT-X2 (Mirus Bio) for 24h ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (55) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Biochemistry 2021Quote: ... lentiviral particles were generated by transient transfection of the lentiviral packaging Lenti-X 293T cells (Takahara) with the TCR encoding plasmids and packaging plasmids (VSVg and PSPAX2) 25 using Transit LT-1 (Mirus). Lentiviral supernatant was harvested 2 days later ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells were maintained in DMEM supplemented with FBS and penicillin/streptomycin and transfected using TransIT-LT1 (Mirus Bio. LLC) with the plasmid containing the sequence of NL-1 or CD4 and incubated overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcribed full-length CHIKV mRNA and mRNA encoding single viral proteins was transfected into target cells with the TransIT mRNA kit (Mirus). MAYV (strain TRVL15537 ...
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bioRxiv - Neuroscience 2021Quote: TDP-43 inducible knockdown SH-SY5Y cells were electroporated with 2 μg of DNA with the Ingenio electroporation kit (Mirus) using the A-023 setting on an Amaxa II nucleofector (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: Lentivirus were generated by transfecting HEK293T cells with standard packaging vectors using TransIT-LT1 Transfection Reagent (Mirus Bio, Madison, WI) or Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were grown in 10-cm dishes and allowed to reach ~70% confluency before transient transfection using TransIT-2020 (Mirus), according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... the above-described PiggyBac-derived plasmid was transfected into KO Fhl1 HEK 293T cell line using TransIT-X2 reagent (Mirus). Next day ...
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bioRxiv - Biophysics 2020Quote: Lentivirus was generated by transfecting HEK39T cells with standard 4th generation packaging vectors using TransIT-LT1 Transfection Reagent (Mirus Bio). Media was changed 10 hours post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Bait and prey plasmids containing fused fragments were then individually transfected together with VSVG and gag/pol packaging vectors in a ratio of 3:1:1 into HEK293T cells using Trans-IT Transfection reagent (Mirus) to produce retroviruses ...
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bioRxiv - Cancer Biology 2021Quote: ... and RSV-REV in the ratio of (3:1:1:1) into HEK293T cells using Trans-IT transfection reagent (Mirus). Virus-rich supernatant was collected at 72 hr post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral particles were produced by co-transfection of shRNA plasmids with psPAX and pMDG.2 in 293T cells using the Trans-IT LT-1 transfection reagent (Mirus). DM cells were infected by shRNA lentivirus twice via spinoculation in the presence of 5 μg/mL polybrene (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... 1 x 106 Jurkat cells expressing Strep-tagged ADAP1 or GFP were resuspended in 0.1 mL Mirusbio ingenio (Mirus, MIR50115) solution containing 2 μg of TF-responsive reporters driving Firefly luciferase (pGL3-3xAP1-luciferase (Addgene ...
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bioRxiv - Immunology 2022Quote: ... Cells were transiently transfected with 500 ng of total DNA and 1.5 µl of Transit X2 (Mirus Bio, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in RPE1 or HeLa cells for 24–48 h using TransIT®-LT1 Transfection Reagent (Mirus Bio) or Neon Electroporation (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Interactions with codon-optimized MECR- and cMECR-mCh fusions were tested by transfecting 293T cells with mammalian expression constructs using TransIT-X2 (Mirus) for 48 h ...
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bioRxiv - Molecular Biology 2020Quote: FLAG*-tagged 3A plasmid DNA were directly transfected in HeLa cells for performing immunofluorescent imaging and co-immunoaffinity purification assays using TransIT transfection reagent (Mirus) by following the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 10cm-plate cultures of indicated cell lines were transfected/infected with indicated constructs using TransIT-Insect transfection reagent (Mirus, #MIR6100) for S2 cells ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (19) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Genetics 2020Quote: WT or mutant mini-genes were transfected in triplicates into COS-7 and ARPE-19 cells using TransIT-LT1 Transfection Reagent (Mirus). Cells were harvested 36h after transfection and total RNA was extracted using Quick-RNA MiniPrep Plus kit (ZYMO Research) ...
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bioRxiv - Cancer Biology 2020Quote: ... and pMD2.G (VSVG envelope vector) vectors were transfected into HEK293T cells in 94 mm2 dish using TransIT-LT1 Transfection Reagent (Mirus). The supernatants containing virus particles was collected at 72 hours after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Retrovirus was produced by transfection of Lenti-X 293T cells with packaging constructs and viral expression vector using TransIT-293 (Mirus). Viral supernatant was harvested 48hr later transfection and filtered through a 0.45 μM filter ...
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bioRxiv - Cancer Biology 2021Quote: ... with ATRX gRNA or vector control were transfected into newly derived primary soft tissue sarcoma cell lines (KP) using the TransIT-LT1 transfection reagent (MIR2304, Mirus). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... and the corresponding cargo plasmid were transfected into 293T cells with the TransIT-LT1 transfection reagent (Mirus, Cat No. MIR230). 48 hours following transfection ...
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bioRxiv - Immunology 2021Quote: ... purified using a Nucleospin kit (Machery-Nagel) and transfected into 2 mL of 293F cells (0.5 mL/mL) using TransIT-Lenti (Mirus bio). The virus was amplified ...
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bioRxiv - Immunology 2020Quote: ... pMigR1-IRES-Thy1.1 or pMigR1-Cre-IRES-Thy1.1 plasmid (Feng et al., 2015) and pCl-Eco were cotransfected into these cells with TransIT-293 Transfection Reagent (Mirus); 2-3 days after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral vectors were co-transfected with packaging vectors VSVG and gag/pol in a ratio of 3:1:2 into HEK293T cells using Trans-IT Transfection reagent (Mirus). Viral supernatants were collected at 48 and 72 hr post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... A7r5 cells were transfected with two different combinations of mixed siRNA oligonucleotides against CLASP1 and CLASP2 using TransIT-X2 (Mirus). After 72 h ...
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bioRxiv - Genomics 2022Quote: ... Three px330-mCherry-sgRNAs vectors were co-transfected into H3.1 and H3.3 sensor cell lines using TransIT-X2 Transfection Reagent (Mirus Bio, MIR6003) as specified by provider’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 250 ng of DNA (split evenly by mass between the plasmids indicated) using TransIT-LT1 (Mirus). Cells were either incubated for 24 h (untreated) ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK-293 TREx FlpIn EMX1-EGFP reporter cells were cultured in DMEM supplemented with 10% (v/v) FBS and 50 μg/mL hygromycin (Mirus).
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bioRxiv - Immunology 2022Quote: ... were seeded in 15-cm2 dishes at a density of 10L×L106 cells per dish and the following day were transfected with 25Lµg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The mix of 47 RTU plasmids was transiently cotransfected in a suspension of assay cells using TransIT-LT1 reagent (Mirus). The transfected cells were plated into 12-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... KB2P tumor-derived cells were transduced with the cloned lentiCRISPR v2 constructs and KB1P cells were transfected with the generated pX330.puro plasmids using a transfection reagent (TransIT-LT1 from Mirus) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... COS-7 cells cultured on coverslips were transfected with the indicated expression vectors using TransIT-LT1 Transfection Reagent (Mirus bio) and maintained for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligation reaction (10 μL) was mixed with 106 BHK-21 cells in 100 μL of Ingenio® Electroporation Solution (Mirus) and transferred to a 0.2 cm cuvette (Mirus) ...
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bioRxiv - Molecular Biology 2023Quote: ... expression in the indicated cell lines was induced with 1 μg/ml doxycycline and cells were transfected with a pcDNA3-SCAPER-FLAG construct using TransIT293 (Mirus) in 10 cm dishes ...
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bioRxiv - Molecular Biology 2023Quote: ... pAC5.1 vectors of CLIP-CRYΔ and TIM- SNAP-HA-ALFA were delivered into S2 cells using the TransIT-Insect Transfection Reagent (Cat# MIR6100, Mirus) following a modified version of the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-Lenti Trasfection Reagent (6605, Mirus). CH157-MN cells were stably transduced with lentiviral particles to generate CH-157MN NOTCH3ICD or empty pLVX vector (EV ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with Renilla and Firefly fusion constructs (WT, D120N, E145Q) using TransIT®- LT1 Transfection Reagent (Mirus Bio) according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2023Quote: Retrovirus was generated by transfecting the Plat-E packaging cell line with 2.5 μg DNA and 7.5 μl TransIT-X2 Reagent (Mirus, MIR 600). B cells were isolated and cultured in complate 1640 RPMI medium before transduction ...
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bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Sanger sequencing-verified pX330 plasmids containing the correct sequences of gRNAs were transfected into KB1P-G3 cells using the TransIT-LT1 transfection reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... The cells were transfected at a confluency of ∼80% with 600-1000 ng of plasmid and 6 uL of TransIT293T (Mirus) transfection reagent ...
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bioRxiv - Biochemistry 2023Quote: ... The pCS2 NLS-mCherry-PRIMPOL WT or S255D vectors were used for transfection in U2OS PRIMPOL KO cells using TransIT (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Lentiviruses for transduction of LTR-mCherry reporter and expression of HIV receptors (CD4, CXCR4, and CCR5) was generated by transfection of HEK293 cells using the Mirus TransIT transfection kit (Mirus). The resulting supernatant was cleared of debris by low-speed centrifugation (300g for 5 minutes ...