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Citations for Mirus Bio :
201 - 238 of 238 citations for ssc mir 17 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Cells were seeding onto 48-well plates at a density of 7.5×104 per well and then transfected with 0.3 μg in-vitro-transcribed RNA using a TransIT-mRNA transfection kit (Mirus) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: Cells were seeded onto 48-well plates at a density of 7.5×104 per well and then transfected with 0.3 μg in-vitro transcribed RNA using a TransIT-mRNA transfection kit (Mirus) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... Bare mRNA encoding CH505 M5 gp160 Envs were transfected at 0.5 μg/million cells using TransIT-mRNA Transfection Kit (Mirus). Transfected cells were harvested 48 hr post transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and the plasmid was transfected into the cells expressing tagged AP1G1 using a TransIT-HeLaMONSTER kit (Mirus Bio LLC), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.02 µg of each mRNA reporter per well was transfected the next day using the TransIT-mRNA Transfection Kit (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (55) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcribed full-length CHIKV mRNA and mRNA encoding single viral proteins was transfected into target cells with the TransIT mRNA kit (Mirus). MAYV (strain TRVL15537 ...
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bioRxiv - Neuroscience 2021Quote: TDP-43 inducible knockdown SH-SY5Y cells were electroporated with 2 μg of DNA with the Ingenio electroporation kit (Mirus) using the A-023 setting on an Amaxa II nucleofector (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... Aag2 and U4.4 cells were transfected with 100 ng dsRNA and 250 ng RepDV2Rluc RNA per well using the TransIT-mRNA transfection kit (Mirus). As a positive control ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr ...
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bioRxiv - Immunology 2020Quote: ... Cells were infected with AD169r-GFP at an MOI of 1.0 or transfected with gB-encoding mRNA using the TransIT mRNA transfection kit (Mirus Biosciencies), then incubated a further 48 hours at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (19) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Immunology 2021Quote: ... purified using a Nucleospin kit (Machery-Nagel) and transfected into 2 mL of 293F cells (0.5 mL/mL) using TransIT-Lenti (Mirus bio). The virus was amplified ...
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bioRxiv - Immunology 2021Quote: ... 293T-ACE2 cells seeded in 24-well plates (100,000 cells/well) were transfected with 250ng of plasmid (Empty vector, p42, p46, and p46 ATIL) using the TransIT X2 kit (Mirus). A duplicate plate was transfected at the same time in order to confirm OAS1 expression by immunoblot ...
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bioRxiv - Microbiology 2022Quote: ... Control and KO cells were seeded at 40,000 per 48 well and transfected with 200 μg RNA in 25 μL OptiMEM using Trans-IT mRNA Transfection Kit (Mirus) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Two doses of unformulated mRNA (500 ng/ml and 100 ng/ml) were transfected in parallel using a TransIT-mRNA transfection kit (Mirus). For analysis of membrane-bound antigen expression ...
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bioRxiv - Microbiology 2023Quote: ... Lentiviruses for transduction of LTR-mCherry reporter and expression of HIV receptors (CD4, CXCR4, and CCR5) was generated by transfection of HEK293 cells using the Mirus TransIT transfection kit (Mirus). The resulting supernatant was cleared of debris by low-speed centrifugation (300g for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... viral RNA isolated from infected BHK cells was transfected into cells at a concentration of 500 µg/ml using TransIT-mRNA transfection kit (Mirus). RABVLe RNA (2 pmol/ml ...
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bioRxiv - Neuroscience 2021Quote: ... primary cortical neurons were transfected by nucleofection using Nucleofector™ II (Amaxa) and the Ingenio® Electroporation kit (Mirus Bio LLC). For one well of a 6-well plate ...
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bioRxiv - Biochemistry 2022Quote: ... in 12-well plates and were transfected with 3 μg capped in vitro transcribed DENV replicon RNA using the TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... BHK-21 cells cultured in 12-well plates were transfected with 3 μg capped in vitro transcribed DENV2 RNAs using the TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... scDNAs were Cy5-labeled using the Label IT® Nucleic Acid Labeling Kit (Mirus Bio LLC, Madison, WI, USA; WI 53719), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Cells were seeded in 96-well plates one day before transfection with TransIT-PRO transfection kit (Mirus Bio, Madison, WI, US) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... MRC-5 cell monolayers were mock-transfected or transfected with sHCoV genomic RNA (1 μg/ml) using TransIT-mRNA Transfection Kit (Mirus Bio) at 33°C ...
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bioRxiv - Microbiology 2023Quote: ... Huh7 cells in 6- well plates were transfected with 3 μg of each in vitro transcribed RNA using the TransIT mRNA transfection kit (Mirus Bio) as previously described (33) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cy5-labeled probes were generated from RP11-440N18 BAC DNA sonicated to 150 bp and labeled using a DNA labeling kit (Mirus Bio). Slides were denatured with 0.5 M NaOH for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... MEF cells were grown in 6-well plates to 80% confluency before transfection with RP-sgRNA11204G (1.5ug/well) using TransIT®-mRNA Transfection Kit (Mirus Bio LLC). After 24h post transfection ...
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bioRxiv - Microbiology 2020Quote: ... the cells were transfected with 1 μg of in vitro-transcribed J6/JFH-1 Rluc RNA or H77S.3/GLuc RNA using the TransIT mRNA transfection kit (Mirus Bio LLC) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... were used to transfect 1 × 106 Huh-7 cells using the TransIT® mRNA transfection kit according to the manufacturer’s instructions (Mirus Bio LLC). At 72 h post transfection (p.t.) ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of viral genomic RNA was transfected per well in 12-well plates through the TransIT®-mRNA Transfection Kit (Mirus Bio) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... the packaging cell line HEK293-FT was transfected with pTCP-MPsfGFP together with pSAX2 (12260 Adgene) and pCMV-VSV-G (8454 Adgene) plasmids using the kit TransIT®-293 Transfection Reagent (Mirus Bio) and following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... culture medium was replaced with 345 µL fresh DMEM per well and mammalian expression plasmids were transfected into cells using the TransIT-HeLaMONSTER Transfection Kit (Mirus Bio #MIR2904) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Transient transfection with synthetic mRNA of Cas9 and sgRNAs was performed in either MEF or 293K cells using the TransIT®-mRNA Transfection Kit (Mirus Bio LLC) in OptiMEM medium (Invitrogen) ...
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bioRxiv - Immunology 2019Quote: 100 ug of pCMV6 plasmid was labeled with 55 ul of Cy5 Label-IT kit for 1 hour at 37°C (Mirus Bio, Madison, WI). The labeled plasmid was purified by ethanol precipitation ...
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bioRxiv - Immunology 2022Quote: ... or control XRNA (RNA segment derived from the hepatitis C virus genome adjacent to and equivalent in length to polyU/UC that cannot induce RIG-I-dependent innate immune signaling8) using the TransIT®-mRNA Transfection kit (Mirus Bio LLC). Cells were collected in RLT buffer (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... The ctDNA was fluorescently labeled with either Cx-rhodamine or CY5 using the Label IT® Nucleic Acid Labeling kit (Mirus Bio LLC, WI).
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bioRxiv - Synthetic Biology 2023Quote: ... 2.5x105 wildtype K562 cells and 2.5x105 reporter K562 cells were mixed for a total of 5.0x105 cells per well and transfected with various amounts of the purified mRNA using the TransIT®-mRNA Transfection Kit (Mirus Bio, catalog# MIR2250). To induce the reporter cell lines ...
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bioRxiv - Molecular Biology 2023Quote: The Cas9-sgRNA and Cas12a-crRNA were labeled with CX-rhodamine (Label IT® Nucleic Acid Labeling Reagents Rhodamine Kit, Mirus Bio, Madison, WI) (75 ...