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Citations for Mirus Bio :
201 - 250 of 270 citations for 7 CHLOROTHIENO 2 3 F 1 3 BENZODIOXOLE 6 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... These plasmids (1 µg each) were transfected by the TransIT system (Mirus) into 2×106 S2 cells that were plated in 6-well plates one day before analysis ...
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bioRxiv - Neuroscience 2020Quote: ... and 1 μg each of Gβ1 and Gγ2-GFP2 using TransIT-2020 (Mirus) as transfection reagent ...
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bioRxiv - Cell Biology 2021Quote: or from Sigma for universal negative control #1 (SIC001) and were transfected using TransIT-X2 (Mirus) in Opti-MEM media following manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2019Quote: ... in a 40:1 ratio for 48 h using TransIT 2020 (Mirus Bio) or Dharmafect Duo (Dharmacon) ...
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bioRxiv - Immunology 2019Quote: ... for HeLa and THP-1 cells or TransIT-293 (Mirus Bio LLC, Madison, WI) for 293T cells ...
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bioRxiv - Systems Biology 2020Quote: ... Following 1 week of incubation with a selection media containing G418 (Mirus Bio LLC) at 1 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... Following 1 week of incubation with a selection media containing G418 (Mirus Bio LLC) at 1mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... AMO1 shIRE1 cl.1 cells were transfected using TransIT-X2 Dynamic Delivery system (Mirus), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... or non-Targeting shRNA vectors pLKO.1 using Mirus Trans-IT TKO Transfection reagent (Mirus). 621-101 cells were transduced with lentiviruses for 48 hours and then selected against puromycin ...
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bioRxiv - Cell Biology 2024Quote: ... and PEX19 sgRNAs were transfected in 96-well plates by lipofectamine (TransIT LT-1, Mirus). A normalized 55 ng of total plasmid DNA was used at a ratio of 50:5 TOPFlash/FOPFlash:Renilla ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were co-transfected with a lentiviral sgRNA expression plasmid and the packaging vectors pCMVΔ8.91 and pMD VSV-G (1:0.7:0.3 mix) using TransIT-293 (Mirus Bio). HEK293T media was collected 48 h post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... The CPER products were then directly transfected into HEK293-C34 cells using Trans IT LT-1 (Mirus). At 6 hours post-transfection ...
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bioRxiv - Cell Biology 2019Quote: ... 8 µg pCMV-dR8.91 and 1 µg pMD2.G packaging plasmids using 48 µl TransIT-LT1 (Mirus). Plasmid DNA was mixed with transfection reagent in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected with the plasmids by using Trans IT LT-1 transfection reagent (Mirus, Madison, WI) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... seeded on day 0 in a T75 culture flasks and transfected on day 1 with TransIT (Mirus) using 3rd generation packing plasmids (pHDMG·G VSV ENV ...
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bioRxiv - Immunology 2023Quote: ... These DNA mixes were incubated with 18 mL OptiMEM and 1 mL of TransIT-LT1 reagent (Mirus) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... Using either the DharmaFECT 1 Transfection Reagent (T-2001-02, Dharmacon) or the Mirus TransIT-X2® (Mirus) system as per the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2023Quote: ... using TransIT reagent (0.25 µl/well, 96-well plate, and 1 µl/well, 48-well plate, Mirus Bio). The transfection mix was replaced with fresh medium after 4 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 μg mL−1 Streptomycin were passed to 10 cm dishes and co-transfected using TransIT (Mirus Bio) in an approximate 1:2.5 ratio with DRD1 containing C-terminal Renilla luciferase (RLuc ...
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bioRxiv - Genomics 2019Quote: ... 4 to 8-week-old mice were injected intravenously with 1 μg of mRNA formulated with TransIT transfection reagent following the manufacturer’s suggestions (Mirus). The procedure for one mouse was as follows ...
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bioRxiv - Microbiology 2021Quote: A549 ZAP−/−/− and DF-1 cells were cultured in 10 cm dish and transfected using LT1 reagent (Mirus lab) with 5 μg of huZAP-GFP DNA for 24 h ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 Mϕ were transfected with a transfection complex (50 μl) of 0.3% TransIT™-LT1 (Mirus Bio™) transfection reagent (vehicle ...
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bioRxiv - Cell Biology 2022Quote: ... Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus), and Opti-MEM Reduced Serum Medium (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... cultured in Schneider’s Drosophila Medium containing 10% heat-inactivated FBS and 1X penicillin-streptomycin with the TransIT-LT 1 reagent (Mirus). Flag-CG30403 only (no bait ...
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bioRxiv - Cell Biology 2020Quote: ... adherent Sf21 cells were transfected with plasmids expressing ARIF-1 using TransIT-Insect transfection reagent (Mirus Bio, Madison, WI). At 3 d post-transfection ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep) were transfected with 25 μg of plasmid encoding for the corresponding S glycoprotein using TransIT-Lenti (Mirus) as transfectant reagent ...
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bioRxiv - Biophysics 2022Quote: ... and 100 ug ml-1 Streptomycin were passed to 10 cm dishes and co-transfected using TransIT (Mirus Bio) in an approximate 1:2.5 ratio with NTSR1 containing C-terminal Renilla luciferase (RLuc ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected in duplicate into HEK293T cells in a 48-well plate using LT-1 transfection reagent (Mirus) with the indicated plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... pTRIPz or pLKO.1 plasmid was co-transfected with lentiviral Packaging vectors (pMD2.G and psPAX2) into HEK293T using TransIT-LT1 (Mirus) transfection reagent following the manufacturer instruction ...
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bioRxiv - Genomics 2021Quote: ... and pMD2.G at a ratio of 9:8:1 by mass using TransIT-LT1 transfection reagent (Mirus MIR 2306) at a ratio of 3 μL transfection reagent per 1 μg plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... lentiviral particles were generated by transient transfection of the lentiviral packaging Lenti-X 293T cells (Takahara) with the TCR encoding plasmids and packaging plasmids (VSVg and PSPAX2) 25 using Transit LT-1 (Mirus). Lentiviral supernatant was harvested 2 days later ...
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bioRxiv - Microbiology 2021Quote: ... 1 x 106 Jurkat cells expressing Strep-tagged ADAP1 or GFP were resuspended in 0.1 mL Mirusbio ingenio (Mirus, MIR50115) solution containing 2 μg of TF-responsive reporters driving Firefly luciferase (pGL3-3xAP1-luciferase (Addgene ...
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bioRxiv - Microbiology 2020Quote: The CPER products (25 μl out of a 50 μl reaction volume) were transfected into HEK293-3P6C33 cells or BHK-21 cells with Trans IT LT-1 (Mirus), following the manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... pMigR1-IRES-Thy1.1 or pMigR1-Cre-IRES-Thy1.1 plasmid (Feng et al., 2015) and pCl-Eco were cotransfected into these cells with TransIT-293 Transfection Reagent (Mirus); 2-3 days after transfection ...
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bioRxiv - Cell Biology 2022Quote: Plasmids for expression of kinesin-1 or any of the kinesin-6 family motors tagged with monomeric NeonGreen and an FRB domain were cotransfected into COS-7 cells with a plasmid for expression of PEX3-mRFP-FKBP or GMAP210p-mRFP-2xFKBP at a ratio of 5:1 with TransIT-LT1 transfection reagent (Mirus). Eighteen hours after transfection ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 6 well plates (2.0×105 per well) and incubated overnight before transfection with pCAGGS-VP40 using lipofectamin LT-1 (MIR2304; Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Medium was changed 24 h later to 10 ml of 5 % FBS/DMEM and changed again 48 h later to 10 ml of 5 % FBS/DMEM containing 1 µg/ml of puromycin (Mirus). Cells were incubated in selection medium for 72 h ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 ΔENV pseudotyped with VSV-G were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). For HIV-1 ΔENV ΔVpr with Vpr WT packaged in trans (HIV-1ΔVpr +Vpr WT) ...
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bioRxiv - Molecular Biology 2023Quote: ... expression in the indicated cell lines was induced with 1 μg/ml doxycycline and cells were transfected with a pcDNA3-SCAPER-FLAG construct using TransIT293 (Mirus) in 10 cm dishes ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1.5 µg of a pLKO.1-Hygro or lentiCas9-Blast plasmid using the TransIT-LT1 Transfection Reagent (Mirus Bio) as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The dCas9-KRAB-mCherry plasmid was mixed with the pCMV_ΔR8.91 and pMD2.G packaging vectors at a ratio of 9:8:1 with TransIT®-Lenti Transfection Reagent (Mirus) in optiMEM (Gibco ...
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Nucleoprotein reassortment enhanced transmissibility of H3 1990.4.a clade influenza A virus in swinebioRxiv - Microbiology 2023Quote: ... 1 µg of each plasmid was mixed with 18 µl of TransIT-LT1 transfection reagent (Mirus Bio LLC, Madison, WI). The mixture was incubated for 45 min and then used to overlay the 293T/MDCK cells overnight ...
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bioRxiv - Cancer Biology 2021Quote: Retrovirus MSCV and MSCV-NKX2-1 and were produced by transfection of HEK293T cells with TransIT-293 (Mirus Bio, Madison, WI) with the retrovirus backbone as well as packaging vectors gag/pol and CMV-VSV-G ...
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bioRxiv - Bioengineering 2021Quote: ... 1×105 MC38 cells were transfected with gRNA-ligated eSpCas9 plasmids for 48 hours using TransIT-LT1 transfection reagent (Mirus Bio) in Opti-MEM (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... to be integrated into the genomic FRT site was cloned into the pcDNA5/FRT/TO vector backbone and transfected together with pOG44 Flp-In recombinase in a 9:1 ratio using either Trans-IT 293 transfection reagent (Mirus, USA) or Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected overnight with either a control plasmid (PLX) or TMPRSS2 (PLX-TMPRSS2) via TransIT LT-1 transfection reagent (Mirus Bio) in 100 µL of OptiMEM per well ...
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bioRxiv - Immunology 2022Quote: Monolayers of BSC-40 cells were infected with parental IHD-J at an MOI of 0.01 PFU/cell and transfected 1 hour later with 10 μg of DNA of plasmid pCyA-ISG15GG (pCyA was kindly provided by Mariano Esteban) using LT-1 (Mirus Bio) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... a 10 cm dish of cells was transfected with 9 µg of Flp-Recombinase (plasmid pOG44) and 1 µg of a specific pcDNA5/FRT plasmid using TransIT-293 transfection reagent (Mirus, MIR2705). 48 hours after transfection ...