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Citations for Mirus Bio :
101 - 150 of 315 citations for Mouse ONECUT3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Total of 2.49 μg plasmid DNA with 6.25 μL TransIT LT1 transfection reagent (Mirus) in 250 μL Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... and indicated plasmids were delivery into cells by using TransITR-LT1 transfection reagent (Mirus). The culture medium was refreshed at 16 hr and harvested at 48 hr and 72hr post-transfection ...
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bioRxiv - Immunology 2021Quote: Human Alveolus Chips were transfected with plasmid DNA using the TransIT-X2 reagent (Mirus Bio). 3 ug of plasmid DNA and 6 ul TransIT-X2 reagent were constituted in 300 ul OPT-MEM before added to 3 ml chip flow medium ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were transfected into WT or DKO cells using TransIT-LT1 Transfection Reagent (Mirus Bio), and transfected cells were plated in 96-wells the next day for HAV infection ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of plasmids was performed using calcium phosphate reagent or TransIT 2020 reagent from Mirus Bio LLC while siRNA was transfected using the TransIT siquest reagent from Mirus Bio LLC.
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bioRxiv - Molecular Biology 2022Quote: ... luciferase reporter plasmids were transfected into cells by TransIT 2020 transfection reagent (Mirus Bio, MIR5400). 24 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila constructs were transfected with plasmid DNA using TransIT®-Insect transfection reagent (Mirus Bio).
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bioRxiv - Cell Biology 2019Quote: ... and the following day plasmids were transfected using Trans-IT 2020® reagent (Mirus Bio) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: Cells were transfected with the plasmids or siRNAs (40 nM at final concentration) by Mirus TransIT-LT1 (Mirus Bio LLC ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... cells were transfected with0.5 μg of NS4B-encoding plasmids using TransIT-LT1 Transfection Reagent (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and the lentiCRISPR v2 plasmid with the guide of interest using TransIT-Lenti (Mirus Bio). Lentivirus-containing supernatant was collected 48 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... transfected with 3μg of total plasmid using TransIT-LT1 transfection reagent (Mirus, Cat: MIR 2306) following the manufacturer’s protocol with a TransIT-LT1 (μl) ...
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bioRxiv - Cancer Biology 2022Quote: ... Transient plasmid DNA transfection was performed using TransIT®-LT1 Transfection Reagent (Mirus, Madison, WI) 6 ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng pEZY_AR plasmid and either 100 ng pEZY_eGFP or pEZY_Flag_HOXB13 using TransIT-2020 (Mirus) and then plated in DMEM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... transfection of plasmid constructs was performed using Trans-IT 2020 reagent (Mirus Bio, #MIR 5400) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid cocktail was then gently mixed with 30 μL of TransIT LT1 transfection reagent (Mirus). The mixture was incubated at room temperature (RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were transfected into HEK 293T cells using Mirus TransIT 293 (Mirus Bio LLC, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... or Gqi5 reporter plasmid (3 μg/plate) was carried out with TransIT-X2® System (Mirus). The cells were serum-starved for 16 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The transient plasmid DNA transfection was performed using TransIT®-LT1 Transfection Reagent (Mirus, Madisom, WI). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... pCMVΔR8.91 and pMD2VSV-G envelope plasmids into HEK293T cells using TransIT-LTI transfection reagent (Mirus Bio). Virus particles collected at 48 hours post-transfection were passed through a 0.45 μm filter and added to SVOG3e cells at a M.O.I ...
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bioRxiv - Cell Biology 2022Quote: ... and vector plasmids were cotransfected into HEK293T cells using TransIT®-293 (Mirus Bio, Madison, WI). Lentiviral supernatants were harvested at 48-72 hrs ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with plasmids encoding CD4-SIV Env chimeras (22) using TransIT-HeLaMONSTER (Mirus) and stable transfectants were selected with 400 μg/ml G418 Sulphate (Calbiochem) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 675 ng of plasmid expressing gRNA were transfected using 7.5 µL of TransIT-X2 (Mirus) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and 110 ng of anti-CRISPR expression plasmids with 1.25 µl of TransIT-X2 (Mirus Bio) in 20 µl Opti-MEM ...
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bioRxiv - Immunology 2021Quote: ... The plasmid cocktail was then gently mixed with 15 μL of TransIT LT1 transfection reagent (Mirus). The growth media were replaced with opti-MEM during transfection ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transfected with the ISARL expression plasmid (pEZT-ISARL) using TransIT 2020 (Mirus, #MIR5404). After 40 h post transfection ...
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bioRxiv - Immunology 2021Quote: ... Cultures were then transfected with various S expression plasmids using TransIT-Lenti transfection reagent (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Infected cells were transfected with the transfer plasmid using TransIT-LT1 (Mirus Bio, Madison, WI, USA). Recombinant viruses BeninΔA179L expressing the β-GUS gene were identified by incubation with 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid and purified by limiting dilution ...
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bioRxiv - Cell Biology 2023Quote: ... individual wells were transfected with plasmids encoding Venus fused BH3-proteins using TransIT-X2 reagent (Mirus). Non-transfected wells were treated with transfection reagent alone (no DNA added) ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid transfections to generate stable cell lines were performed with TransIT®-LT1 transfection reagent (Mirus). 2×105 cells per well were seeded in 6-well plates the day before transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Kc cells were transiently transfected with 400ng of each plasmid using the TransIT-2020 reagent (Mirus). Live imaging was performed 72 hours after transfection.
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: The lentiviral constructs were co-transfected with pCMV-dR8.91 and pMD2.D plasmids using TransIT-Lenti (Mirus) into 293T cells ...
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bioRxiv - Genetics 2021Quote: ... 1ug of plasmid was transfected per 1ml of cells using TransIT®-Insect Transfection Reagent (Mirus, #6100) as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... protein expression (15 ng/well) and target (4.5 ng/well) plasmids using TransIT-2020 Transfection Reagent (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Lentivirus was generated through the triple transfection of HEK 293T cells with the lentiviral transfer vector plus the two packaging plasmids pCMVΔR8.91 and pMD.G using TransIT-293 transfection reagent (Mirus) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Confirmed plasmids were transformed into S2 cells using TransIT Insect Transfection Reagent (Mirus Bio, Madison, WI, USA). Transformed cells were grown for 48 h before use in experiments.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids of K14-rtTA and pTRE2-Arhgef11CA were co-transfected into keratinocytes using Mirus transfection reagents (Mirus). 12 hours after infection ...
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bioRxiv - Cancer Biology 2020Quote: ... LNCaP cells were co-transfected with 500ng reporter plasmid and 5ng of Renilla using TransIT-2020 (Mirus) and plated in phenol red-free RPMI (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Szczesny) and 0.8 μg EGFP-CHIP plasmid using Mirus reagents: 2 ul TransIT-293 (MIR 2700, Mirus) for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900 ...
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bioRxiv - Cell Biology 2022Quote: ... 1ug of spike plasmid for each well was transfected into cells using transfection reagent (Mirus, TransIT-LT1) with adding DMSO or antiviral compounds for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Positive recombinant plasmid was transfected into Sf21 cells using the Flashbac Ultra Baculovirus Expression System (Mirus Bio). Viral Titers were determined with the BacPak qpcr Titration kit (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... All transfections used 2.49 μg plasmid DNA with 6.25 μL TransIT LT1 transfection reagent (Mirus, Madison, WI) in 250 μL Opti-MEM (Gibco).
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bioRxiv - Microbiology 2021Quote: ... and the indicated plasmids were then delivered into the cells using TransIT®-LT1 transfection reagent (Mirus). The culture medium was refreshed at 16 h and then harvested at 48 h and 72 h post-transfection ...
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bioRxiv - Cell Biology 2019Quote: ... 8 µg pCMV-dR8.91 and 1 µg pMD2.G packaging plasmids using 48 µl TransIT-LT1 (Mirus). Plasmid DNA was mixed with transfection reagent in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... All transfections used 2.49 μg plasmid DNA with 6.25 μL TransIT LT1 transfection reagent (Mirus, Madison, WI), in 250 μL Opti-MEM (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected with the plasmids by using Trans IT LT-1 transfection reagent (Mirus, Madison, WI) according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2019Quote: ... The LCLs were transfected with a plasmid using Trans-IT XP transfection reagent (Mirus Bio, Madison, WI) and treated with a demethylating agent ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 250 ng of plasmid expressing HA-tagged M polyprotein using TransIT-LT1 (Mirus) and after 6 h cells were treated with 1 µM Pon A or DMSO as a control ...
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bioRxiv - Systems Biology 2023Quote: The DNA library was co-transfected with pCMV-dR8.91 and pMD2.G plasmids using TransIT-Lenti (Mirus) into HEK293 cells ...
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bioRxiv - Microbiology 2023Quote: ... All transfections used 2.49 µg plasmid DNA with 6.25 µL TransIT LT1 transfection reagent (Mirus, Madison, WI) in 250 µL Opti-MEM (Gibco) ...